Restriction map for pF1KA0660
 (Linear) (Six-base) MAPSORT of: KIAA0660.seq  Check: 4683  from: 1  to: 1446



KIAA0660.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AclI AA'CG_TT

Cuts at:      0    1067    1446 
   Size:       1067     379
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     368     638     725    1446 
   Size:        192     176     270      87     721

  Fragments arranged by size: 

                 721     270     192     176      87
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     524    1446 
   Size:        524     922
 
AlwNI CAG_nnn'CTG

Cuts at:      0     604     697    1446 
   Size:        604      93     749

  Fragments arranged by size: 

                 749     604      93
 
ApoI r'AATT_y

Cuts at:      0     108     227     367     791    1045    1116    1446 
   Size:        108     119     140     424     254      71     330

  Fragments arranged by size: 

                 424     330     254     140     119     108      71
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      11    1446 
   Size:         11    1435
 
Bpu10I CC'TnA_GC

Cuts at:      0     593    1446 
   Size:        593     853
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     276    1446 
   Size:        276    1170
 
BsaAI yAC'GTr

Cuts at:      0    1312    1446 
   Size:       1312     134
 
BsaBI GATnn'nnATC

Cuts at:      0     989    1446 
   Size:        989     457
 
BsaWI w'CCGG_w

Cuts at:      0      78    1446 
   Size:         78    1368
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     257     287     666     669     696     699    1446 
   Size:        257      30     379       3      27       3     747

  Fragments arranged by size: 

                 747     379     257      30      27       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     571     655     671     674     679     947    1352    1446 
   Size:        571      84      16       3       5     268     405      94

  Fragments arranged by size: 

                 571     405     268      94      84      16       5       3
            
 
BspEI T'CCGG_A

Cuts at:      0      78    1446 
   Size:         78    1368
 
BspHI T'CATG_A

Cuts at:      0     579    1052    1446 
   Size:        579     473     394

  Fragments arranged by size: 

                 579     473     394
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     767    1446 
   Size:        767     679
 
BsrDI GCAATG_nn'

Cuts at:      0    1293    1446 
   Size:       1293     153
 
BssSI C'ACGA_G

Cuts at:      0     866    1446 
   Size:        866     580
 
BstYI r'GATC_y

Cuts at:      0     352    1446 
   Size:        352    1094
 
DraI TTT'AAA

Cuts at:      0    1205    1446 
   Size:       1205     241
 
DraIII CAC_nnn'GTG

Cuts at:      0     540     868    1446 
   Size:        540     328     578

  Fragments arranged by size: 

                 578     540     328
 
EaeI y'GGCC_r

Cuts at:      0     168    1446 
   Size:        168    1278
 
EarI CTCTTCn'nnn_

Cuts at:      0     455     614    1208    1347    1395    1446 
   Size:        455     159     594     139      48      51

  Fragments arranged by size: 

                 594     455     159     139      51      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     368     638     725    1446 
   Size:        192     176     270      87     721

  Fragments arranged by size: 

                 721     270     192     176      87
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     911    1446 
   Size:        911     535
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1297    1357    1446 
   Size:       1297      60      89

  Fragments arranged by size: 

                1297      89      60
 
EcoRI G'AATT_C

Cuts at:      0     108     791    1045    1446 
   Size:        108     683     254     401

  Fragments arranged by size: 

                 683     401     254     108
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1065    1446 
   Size:       1065     381
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     257     289     427     459     586     618     655     687 
   Size:        257      32     138      32     127      32      37      32

Cuts at:    687    1326    1358    1446 
   Size:        639      32      88

  Fragments arranged by size: 

                 639     257     138     127      88      37      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1108    1446 
   Size:       1108     338
 
MscI TGG'CCA

Cuts at:      0     170    1446 
   Size:        170    1276
 
MslI CAynn'nnrTG

Cuts at:      0     239    1446 
   Size:        239    1207
 
MspA1I CmG'CkG

Cuts at:      0      24    1446 
   Size:         24    1422
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     282    1120    1372    1446 
   Size:        282     838     252      74

  Fragments arranged by size: 

                 838     282     252      74
 
PmlI CAC'GTG

Cuts at:      0    1312    1446 
   Size:       1312     134
 
PpuMI rG'GwC_Cy

Cuts at:      0    1297    1357    1446 
   Size:       1297      60      89

  Fragments arranged by size: 

                1297      89      60
 
SapI GCTCTTCn'nnn_

Cuts at:      0     614    1446 
   Size:        614     832
 
SexAI A'CCwGG_T

Cuts at:      0     897    1446 
   Size:        897     549
 
SmlI C'TyrA_G

Cuts at:      0     255    1446 
   Size:        255    1191
 
SspI AAT'ATT

Cuts at:      0      55      85    1446 
   Size:         55      30    1361

  Fragments arranged by size: 

                1361      55      30
 
StyI C'CwwG_G

Cuts at:      0    1091    1366    1446 
   Size:       1091     275      80

  Fragments arranged by size: 

                1091     275      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1312    1446 
   Size:       1312     134
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1055    1087    1446 
   Size:       1055      32     359

  Fragments arranged by size: 

                1055     359      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1439    1446 
   Size:       1439       7
 
XmnI GAAnn'nnTTC

Cuts at:      0    1151    1446 
   Size:       1151     295
 

 Enzymes that do cut:

     AclI     AcuI     AlfI    AlwNI     ApoI     BmrI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BspEI    BspHI    BspMI
    BsrDI    BssSI    BstYI     DraI   DraIII     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    Hin4I  HindIII     MscI     MslI
   MspA1I    PflMI     PmlI    PpuMI     SapI    SexAI     SmlI     SspI
     StyI    TaqII     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI     BsaI    BsaHI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BsrBI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI     EagI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TatI     XbaI     XcmI     XhoI     XmaI     ZraI
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