Restriction map for pF1KA0622
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        Alw44I      
AlwNI       ApaLI       AscI        AseI        AsnI        
Asp718I     AviII       BbeI        BbrPI       BcgI        
BciVI       BglI        BlpI        Bpu1102I    BsaWI       
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       Bst1107I    BstZ17I     CelII       
ClaI        CpoI        DraIII      DrdI        EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
KasI        KpnI        MfeI        MluI        MroI        
MunI        NarI        NheI        NotI        NruI        
NsiI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        RcaI        RsrII       
SacI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SpeI        Sse8387I    SspBI       StuI        
SwaI        XcmI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         4162  GTCGAC               GT^[AC][GT]AC
AclI          805  AACGTT               AA^CGTT
AflII        2722  CTTAAG               C^TTAAG
AocI         1907  CCTTAGG              CC^T[ACGT]AGG
Aor51HI      2402  AGCGCT               AGC^GCT
ApaI         4334  GGGCCC               GGGCC^C
Asp700I      2512  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        1327  CCTAGG               C^CTAGG
BbvCI        3994  GCTGAGG              CCTCAGC(-5/-2)
BclI         2808  TGATCA               T^GATCA
BfrI         2722  CTTAAG               C^TTAAG
BlnI         1327  CCTAGG               C^CTAGG
BsaBI        2018  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         3121  CTGCAC               GTGCAG(16/14)
BsiEI        4073  CGGCCG               CG[GA][CT]^CG
BsmBI        3656  GAGACG               CGTCTC(1/5)
BspLU11I      588  ACATGT               A^CATGT
BsrDI        4145  CATTGC               GCAATG(2/0)
BssHII       3534  GCGCGC               G^CGCGC
BssSI        2114  CACGAG               CACGAG(-5/-1)
BstBI        3356  TTCGAA               TT^CGAA
BstEII        231  GGTGACC              G^GT[ACGT]ACC
BstPI         231  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1907  CCTTAGG              CC^T[ACGT]AGG
EagI         4073  CGGCCG               C^GGCCG
EclXI        4073  CGGCCG               C^GGCCG
Eco47III     2402  AGCGCT               AGC^GCT
Eco52I       4073  CGGCCG               C^GGCCG
Eco81I       1907  CCTTAGG              CC^T[ACGT]AGG
EcoO65I       231  GGTGACC              G^GT[ACGT]ACC
EcoRI        3138  GAATTC               G^AATTC
FbaI         2808  TGATCA               T^GATCA
KspI         3550  CCGCGG               CCGC^GG
MamI         2018  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          827  GCCGGC               GCC^GGC
NdeI         2408  CATATG               CA^TATG
NgoMIV        827  GCCGGC               G^CCGGC
NspV         3356  TTCGAA               TT^CGAA
PaeR7I        428  CTCGAG               C^TCGAG
PflMI        1575  CCACACCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4113  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      805  AACGTT               AA^CGTT
PvuII         863  CAGCTG               CAG^CTG
SacII        3550  CCGCGG               CCGC^GG
SalI         4162  GTCGAC               G^TCGAC
SapI         2541  GAAGAGC              GCTCTTC(1/4)
SfuI         3356  TTCGAA               TT^CGAA
SmaI         3543  CCCGGG               CCC^GGG
SnaBI         379  TACGTA               TAC^GTA
SphI         3266  GCATGC               GCATG^C
SspI          987  AATATT               AAT^ATT
Van91I       1575  CCACACCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          428  CTCGAG               C^TCGAG
XmaCI        3543  CCCGGG               C^CCGGG
XmaI         3543  CCCGGG               C^CCGGG
XmnI         2512  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI         1860  GACGCC               G[GA]^CG[CT]C
             3186  GACGCC              
AflIII        588  ACATGT               A^C[GA][CT]GT
              600  ACGTGT              
AhdI          214  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1135  GACCTGCGGTC         
AspEI         214  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1135  GACCTGCGGTC         
AspI         1773  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2130  GACACCGTC           
AvaI          428  CTCGAG               C^[CT]CG[GA]G
             3543  CCCGGG              
BanI         3079  GGTGCC               G^G[CT][GA]CC
             3106  GGTGCC              
BsaAI         379  TACGTA               [CT]AC^GT[GA]
             3320  TACGTG              
BsaHI        1860  GACGCC               G[GA]^CG[CT]C
             3186  GACGCC              
BsoBI         428  CTCGAG               C^[CT]CG[GA]G
             3543  CCCGGG              
BsrBI        1659  CCGCTC               CCGCTC(-3/-3)
             3989  GAGCGG              
BsrFI         827  GCCGGC               [GA]^CCGG[CT]
             2051  GCCGGT              
BstAPI         30  GCAGGTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1819  GCAGCCAGTGC         
Cfr10I        827  GCCGGC               [GA]^CCGG[CT]
             2051  GCCGGT              
DraI         1117  TTTAAA               TTT^AAA
             3916  TTTAAA              
Eam1105I      214  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1135  GACCTGCGGTC         
EarI          895  GAAGAG               CTCTTC(1/4)
             2541  GAAGAG              
EcoNI          24  CCTGGCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3615  CCTGAAAGAGG         
Hin1I        1860  GACGCC               G[GA]^CG[CT]C
             3186  GACGCC              
HincII       1767  GTCAAC               GT[CT]^[GA]AC
             4162  GTCGAC              
HindII       1767  GTCAAC               GT[CT]^[GA]AC
             4162  GTCGAC              
Ksp632I       895  GAAGAG               CTCTTC(1/4)
             2541  GAAGAG              
NcoI         1212  CCATGG               C^CATGG
             3246  CCATGG              
NspI          588  ACATGT               [GA]CATG^[CT]
             3266  GCATGC              
PflFI        1773  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2130  GACACCGTC           
PpuMI        1133  AGGACCT              [GA]G^G[AT]CC[CT]
             1777  GGGTCCC             
SexAI        2244  ACCTGGT              A^CC[AT]GGT
             3680  ACCTGGT             
Tth111I      1773  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2130  GACACCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BalI        
BamHI       BanII       BbsI        BbvI        BcnI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XbaI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com