Restriction map for pF1KA0622
 (Linear) (Six-base) MAPSORT of: KIAA0622.seq  Check: 2036  from: 1  to: 4389



KIAA0622.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      21    4389 
   Size:         21    4368
 
AccI GT'mk_AC

Cuts at:      0    4163    4389 
   Size:       4163     226
 
AclI AA'CG_TT

Cuts at:      0     806    4389 
   Size:        806    3583
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     845    1241    1533    1639    2123    2560    3320    4389 
   Size:        845     396     292     106     484     437     760    1069

  Fragments arranged by size: 

                1069     845     760     484     437     396     292     106
            
 
AfeI AGC'GCT

Cuts at:      0    2404    4389 
   Size:       2404    1985
 
AflII C'TTAA_G

Cuts at:      0    2722    4389 
   Size:       2722    1667
 
AflIII A'CryG_T

Cuts at:      0     588     600    4389 
   Size:        588      12    3789

  Fragments arranged by size: 

                3789     588      12
 
AhdI GACnn_n'nnGTC

Cuts at:      0     219    1140    4389 
   Size:        219     921    3249

  Fragments arranged by size: 

                3249     921     219
 
AleI CACnn'nnGTG

Cuts at:      0    3154    4389 
   Size:       3154    1235
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     292     520    4066    4389 
   Size:        292     228    3546     323

  Fragments arranged by size: 

                3546     323     292     228
 
ApaI G_GGCC'C

Cuts at:      0    4338    4389 
   Size:       4338      51
 
ApoI r'AATT_y

Cuts at:      0     417     578     664     717    1396    3061    3138    3890 
   Size:        417     161      86      53     679    1665      77     752

Cuts at:   3890    4389 
   Size:        499

  Fragments arranged by size: 

                1665     752     679     499     417     161      86      77
                  53
 
AvaI C'yCGr_G

Cuts at:      0     428    3543    4389 
   Size:        428    3115     846

  Fragments arranged by size: 

                3115     846     428
 
AvrII C'CTAG_G

Cuts at:      0    1327    4389 
   Size:       1327    3062
 
BamHI G'GATC_C

Cuts at:      0     850    1916    2919    4389 
   Size:        850    1066    1003    1470

  Fragments arranged by size: 

                1470    1066    1003     850
 
BanI G'GyrC_C

Cuts at:      0    3079    3106    4389 
   Size:       3079      27    1283

  Fragments arranged by size: 

                3079    1283      27
 
BanII G_rGCy'C

Cuts at:      0    2110    3163    3747    4338    4389 
   Size:       2110    1053     584     591      51

  Fragments arranged by size: 

                2110    1053     591     584      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     851    2129    3782    3952    4271    4389 
   Size:        851    1278    1653     170     319     118

  Fragments arranged by size: 

                1653    1278     851     319     170     118
 
BbvCI CC'TCA_GC

Cuts at:      0    3995    4389 
   Size:       3995     394
 
BclI T'GATC_A

Cuts at:      0    2808    4389 
   Size:       2808    1581
 
BglII A'GATC_T

Cuts at:      0     612    1045    2023    2286    2588    3369    4389 
   Size:        612     433     978     263     302     781    1020

  Fragments arranged by size: 

                1020     978     781     612     433     302     263
 
Bme1580I G_kGCm'C

Cuts at:      0    1858    3665    4338    4389 
   Size:       1858    1807     673      51

  Fragments arranged by size: 

                1858    1807     673      51
 
BmgBI CAC'GTC

Cuts at:      0    3006    3660    4389 
   Size:       3006     654     729

  Fragments arranged by size: 

                3006     729     654
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     369    1539    1784    2111    4389 
   Size:        369    1170     245     327    2278

  Fragments arranged by size: 

                2278    1170     369     327     245
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     494    1768    1874    2400    3772    3850    4389 
   Size:        494    1274     106     526    1372      78     539

  Fragments arranged by size: 

                1372    1274     539     526     494     106      78
 
Bpu10I CC'TnA_GC

Cuts at:      0    1130    3995    4389 
   Size:       1130    2865     394

  Fragments arranged by size: 

                2865    1130     394
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     979    1661    2370    2851    3118    3745    4389 
   Size:        139     840     682     709     481     267     627     644

  Fragments arranged by size: 

                 840     709     682     644     627     481     267     139
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     947    1148    3826    4389 
   Size:        947     201    2678     563

  Fragments arranged by size: 

                2678     947     563     201
 
BsaAI yAC'GTr

Cuts at:      0     381    3322    4389 
   Size:        381    2941    1067

  Fragments arranged by size: 

                2941    1067     381
 
BsaBI GATnn'nnATC

Cuts at:      0    2022    4389 
   Size:       2022    2367
 
BsaHI Gr'CG_yC

Cuts at:      0    1861    3187    4389 
   Size:       1861    1326    1202

  Fragments arranged by size: 

                1861    1326    1202
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     587     617    2841    2871    3513    3543    3952    3982 
   Size:        587      30    2224      30     642      30     409      30

Cuts at:   3982    3991    4021    4345    4375    4389 
   Size:          9      30     324      30      14

  Fragments arranged by size: 

                2224     642     587     409     324      30      30      30
                  30      30      30      14       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1833    2100    2343    2346    3811    4012    4353    4356 
   Size:       1833     267     243       3    1465     201     341       3

Cuts at:   4356    4389 
   Size:         33

  Fragments arranged by size: 

                1833    1465     341     267     243     201      33       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     204    2012    3524    4389 
   Size:        204    1808    1512     865

  Fragments arranged by size: 

                1808    1512     865     204
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3106    4389 
   Size:       3106    1283
 
BsiEI CG_ry'CG

Cuts at:      0    4076    4389 
   Size:       4076     313
 
BsiHKAI G_wGCw'C

Cuts at:      0     480    1568    2278    3803    3867    4055    4389 
   Size:        480    1088     710    1525      64     188     334

  Fragments arranged by size: 

                1525    1088     710     480     334     188      64
 
BsmI GAATG_Cn'

Cuts at:      0     398    2262    2429    4389 
   Size:        398    1864     167    1960

  Fragments arranged by size: 

                1960    1864     398     167
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3650    4389 
   Size:       3650     739
 
Bsp1286I G_dGCh'C

Cuts at:      0     480    1568    1858    2110    2278    3163    3665    3747 
   Size:        480    1088     290     252     168     885     502      82

Cuts at:   3747    3803    3867    4055    4338    4389 
   Size:         56      64     188     283      51

  Fragments arranged by size: 

                1088     885     502     480     290     283     252     188
                 168      82      64      56      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      21      54    1145    4389 
   Size:         21      33    1091    3244

  Fragments arranged by size: 

                3244    1091      33      21
 
BsrBI CCG'CTC

Cuts at:      0    1661    3991    4389 
   Size:       1661    2330     398

  Fragments arranged by size: 

                2330    1661     398
 
BsrDI GCAATG_nn'

Cuts at:      0    4144    4389 
   Size:       4144     245
 
BsrFI r'CCGG_y

Cuts at:      0     827    2051    4389 
   Size:        827    1224    2338

  Fragments arranged by size: 

                2338    1224     827
 
BssHII G'CGCG_C

Cuts at:      0    3534    4389 
   Size:       3534     855
 
BssSI C'ACGA_G

Cuts at:      0    2114    4389 
   Size:       2114    2275
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      36    1825    4389 
   Size:         36    1789    2564

  Fragments arranged by size: 

                2564    1789      36
 
BstBI TT'CG_AA

Cuts at:      0    3357    4389 
   Size:       3357    1032
 
BstEII G'GTnAC_C

Cuts at:      0     231    4389 
   Size:        231    4158
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     685    1272    2916    4389 
   Size:        685     587    1644    1473

  Fragments arranged by size: 

                1644    1473     685     587
 
BstYI r'GATC_y

Cuts at:      0     531     612     850    1045    1916    2023    2286    2588 
   Size:        531      81     238     195     871     107     263     302

Cuts at:   2588    2749    2919    3369    4389 
   Size:        161     170     450    1020

  Fragments arranged by size: 

                1020     871     531     450     302     263     238     195
                 170     161     107      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1908    4389 
   Size:       1908    2481
 
BtgI C'CryG_G

Cuts at:      0    1212    3246    3550    4389 
   Size:       1212    2034     304     839

  Fragments arranged by size: 

                2034    1212     839     304
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3440    4389 
   Size:       3440     949
 
BtsI GCAGTG_nn'

Cuts at:      0    4045    4389 
   Size:       4045     344
 
DraI TTT'AAA

Cuts at:      0    1119    3918    4389 
   Size:       1119    2799     471

  Fragments arranged by size: 

                2799    1119     471
 
EaeI y'GGCC_r

Cuts at:      0      69    1274    2226    2906    3180    4016    4073    4389 
   Size:         69    1205     952     680     274     836      57     316

  Fragments arranged by size: 

                1205     952     836     680     316     274      69      57
            
 
EagI C'GGCC_G

Cuts at:      0    4073    4389 
   Size:       4073     316
 
EarI CTCTTCn'nnn_

Cuts at:      0     890    2536    4389 
   Size:        890    1646    1853

  Fragments arranged by size: 

                1853    1646     890
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     213    4389 
   Size:        213    4176
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     494     845    1241    1533    1639    1768    1874    2123 
   Size:        494     351     396     292     106     129     106     249

Cuts at:   2123    2400    2560    3320    3772    3850    4389 
   Size:        277     160     760     452      78     539

  Fragments arranged by size: 

                 760     539     494     452     396     351     292     277
                 249     160     129     106     106      78
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      28    3619    4389 
   Size:         28    3591     770

  Fragments arranged by size: 

                3591     770      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1134    1778    4334    4389 
   Size:       1134     644    2556      55

  Fragments arranged by size: 

                2556    1134     644      55
 
EcoRI G'AATT_C

Cuts at:      0    3138    4389 
   Size:       3138    1251
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2533    2713    3799    4389 
   Size:       2533     180    1086     590

  Fragments arranged by size: 

                2533    1086     590     180
 
HaeII r_GCGC'y

Cuts at:      0    1389    2406    3437    4389 
   Size:       1389    1017    1031     952

  Fragments arranged by size: 

                1389    1031    1017     952
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     205     237     342     374     422     454 
   Size:         76      32      97      32     105      32      48      32

Cuts at:    454     597     629    1126    1158    1648    1674    1680    1706 
   Size:        143      32     497      32     490      26       6      26

Cuts at:   1706    2580    2612    3403    3435    3991    4023    4389 
   Size:        874      32     791      32     556      32     366

  Fragments arranged by size: 

                 874     791     556     497     490     366     143     105
                  97      76      48      32      32      32      32      32
                  32      32      32      32      26      26       6
 
HincII GTy'rAC

Cuts at:      0    1769    4164    4389 
   Size:       1769    2395     225

  Fragments arranged by size: 

                2395    1769     225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     789    1894    2947    3734    4389 
   Size:        789    1105    1053     787     655

  Fragments arranged by size: 

                1105    1053     789     787     655
 
MscI TGG'CCA

Cuts at:      0      71    1276    2228    2908    4018    4389 
   Size:         71    1205     952     680    1110     371

  Fragments arranged by size: 

                1205    1110     952     680     371      71
 
MslI CAynn'nnrTG

Cuts at:      0     513    2599    2678    2827    3154    4048    4105    4389 
   Size:        513    2086      79     149     327     894      57     284

  Fragments arranged by size: 

                2086     894     513     327     284     149      79      57
            
 
MspA1I CmG'CkG

Cuts at:      0     865    1698    3552    4389 
   Size:        865     833    1854     837

  Fragments arranged by size: 

                1854     865     837     833
 
NaeI GCC'GGC

Cuts at:      0     829    4389 
   Size:        829    3560
 
NcoI C'CATG_G

Cuts at:      0    1212    3246    4389 
   Size:       1212    2034    1143

  Fragments arranged by size: 

                2034    1212    1143
 
NdeI CA'TA_TG

Cuts at:      0    2409    4389 
   Size:       2409    1980
 
NgoMIV G'CCGG_C

Cuts at:      0     827    4389 
   Size:        827    3562
 
NspI r_CATG'y

Cuts at:      0     592    3270    4389 
   Size:        592    2678    1119

  Fragments arranged by size: 

                2678    1119     592
 
PciI A'CATG_T

Cuts at:      0     588    4389 
   Size:        588    3801
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1581    4389 
   Size:       1581    2808
 
PfoI T'CCnGG_A

Cuts at:      0    2416    3098    4389 
   Size:       2416     682    1291

  Fragments arranged by size: 

                2416    1291     682
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3280    3312    3613    3645    4389 
   Size:       3280      32     301      32     744

  Fragments arranged by size: 

                3280     744     301      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1134    1778    4389 
   Size:       1134     644    2611

  Fragments arranged by size: 

                2611    1134     644
 
PshAI GACnn'nnGTC

Cuts at:      0    4117    4389 
   Size:       4117     272
 
PsiI TTA'TAA

Cuts at:      0    2665    4389 
   Size:       2665    1724
 
PspOMI G'GGCC_C

Cuts at:      0    4334    4389 
   Size:       4334      55
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2453    2485    4389 
   Size:       2453      32    1904

  Fragments arranged by size: 

                2453    1904      32
 
PstI C_TGCA'G

Cuts at:      0     245    1610    1878    2531    4154    4187    4389 
   Size:        245    1365     268     653    1623      33     202

  Fragments arranged by size: 

                1623    1365     653     268     245     202      33
 
PvuII CAG'CTG

Cuts at:      0     865    4389 
   Size:        865    3524
 
SacII CC_GC'GG

Cuts at:      0    3553    4389 
   Size:       3553     836
 
SalI G'TCGA_C

Cuts at:      0    4162    4389 
   Size:       4162     227
 
SanDI GG'GwC_CC

Cuts at:      0    1778    4389 
   Size:       1778    2611
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2536    4389 
   Size:       2536    1853
 
SexAI A'CCwGG_T

Cuts at:      0    2244    3680    4389 
   Size:       2244    1436     709

  Fragments arranged by size: 

                2244    1436     709
 
SfcI C'TryA_G

Cuts at:      0     241     929    1606    1745    1874    2527    3309    4150 
   Size:        241     688     677     139     129     653     782     841

Cuts at:   4150    4183    4389 
   Size:         33     206

  Fragments arranged by size: 

                 841     782     688     677     653     241     206     139
                 129      33
 
SmaI CCC'GGG

Cuts at:      0    3545    4389 
   Size:       3545     844
 
SmlI C'TyrA_G

Cuts at:      0     118     428     958    1676    2385    2722    2866    3133 
   Size:        118     310     530     718     709     337     144     267

Cuts at:   3133    3760    4389 
   Size:        627     629

  Fragments arranged by size: 

                 718     709     629     627     530     337     310     267
                 144     118
 
SnaBI TAC'GTA

Cuts at:      0     381    4389 
   Size:        381    4008
 
SphI G_CATG'C

Cuts at:      0    3270    4389 
   Size:       3270    1119
 
SspI AAT'ATT

Cuts at:      0     989    4389 
   Size:        989    3400
 
StyI C'CwwG_G

Cuts at:      0     779     846    1212    1327    1580    3246    3783    3837 
   Size:        779      67     366     115     253    1666     537      54

Cuts at:   3837    4389 
   Size:        552

  Fragments arranged by size: 

                1666     779     552     537     366     253     115      67
                  54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     162    3246    3538    4389 
   Size:        162    3084     292     851

  Fragments arranged by size: 

                3084     851     292     162
 
TatI w'GTAC_w

Cuts at:      0     933    2281    4389 
   Size:        933    1348    2108

  Fragments arranged by size: 

                2108    1348     933
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     587     619    3513    3545    3952    3984    4389 
   Size:        587      32    2894      32     407      32     405

  Fragments arranged by size: 

                2894     587     407     405      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1776    2133    4389 
   Size:       1776     357    2256

  Fragments arranged by size: 

                2256    1776     357
 
XbaI T'CTAG_A

Cuts at:      0    1735    2778    2965    4389 
   Size:       1735    1043     187    1424

  Fragments arranged by size: 

                1735    1424    1043     187
 
XhoI C'TCGA_G

Cuts at:      0     428    4389 
   Size:        428    3961
 
XmaI C'CCGG_G

Cuts at:      0    3543    4389 
   Size:       3543     846
 
XmnI GAAnn'nnTTC

Cuts at:      0    2516    4389 
   Size:       2516    1873
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbsI    BbvCI     BclI    BglII Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI    PvuII
    SacII     SalI    SanDI     SapI    SexAI     SfcI     SmaI     SmlI
    SnaBI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AgeI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     BaeI     BbeI     BcgI    BciVI    BfrBI     BglI     BlpI
     BmtI     BplI    BsaWI    BsiWI    BspEI    BspHI    BsrGI  BstZ17I
     ClaI    CspCI   DraIII     DrdI  EcoICRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
    PspXI     PvuI    RsrII     SacI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SpeI     SrfI     StuI     SwaI     XcmI     ZraI
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