Restriction map for pF1KA0610
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BcgI        BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BsrFI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     KasI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SnaBI       SpeI        SwaI        
Tth111I     XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        737  GGTACC               G^GTACC
Alw44I        719  GTGCAC               G^TGCAC
AocI          638  CCTTAGG              CC^T[ACGT]AGG
ApaLI         719  GTGCAC               G^TGCAC
Asp700I       563  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       737  GGTACC               G^GTACC
AvaI          796  CCCGGG               C^[CT]CG[GA]G
BanII         961  GAGCTC               G[GA]GC[CT]^C
BbvCI         406  CCTCAGC              CCTCAGC(-5/-2)
BglII         844  AGATCT               A^GATCT
BpmI         1003  CTCCAG               CTGGAG(16/14)
BsaI          634  GAGACC               GGTCTC(1/5)
BsaWI         849  TCCGGT               [AT]^CCGG[AT]
BsmAI         634  GAGAC                GTCTC(1/5)
BsmFI        1425  GGGAC                GGGAC(10/14)
BsoBI         796  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1713  TGTACA               T^GTACA
BsrBI        1372  GAGCGG               CCGCTC(-3/-3)
BsrDI        1831  GCAATG               GCAATG(2/0)
BsrGI        1713  TGTACA               T^GTACA
BstAPI        454  GCAGTTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         743  TTCGAA               TT^CGAA
BstXI        1044  CCAAATCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        638  CCTTAGG              CC^T[ACGT]AGG
DraII         201  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI         1397  CCGTGG               C^C[GA][CT]GG
Eco81I        638  CCTTAGG              CC^T[ACGT]AGG
EcoO109I      201  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1039  GAATTC               G^AATTC
EcoT22I      1683  ATGCAT               ATGCA^T
FauI          868  GCGGG                CCCGC(4/6)
KpnI          737  GGTACC               GGTAC^C
MslI         1098  CACTGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1412  CAGCTG               C[AC]G^C[GT]G
NsiI         1683  ATGCAT               ATGCA^T
NspV          743  TTCGAA               TT^CGAA
PpuMI         201  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII        1412  CAGCTG               CAG^CTG
SacI          961  GAGCTC               GAGCT^C
SbfI          703  CCTGCAGG             CCTGCA^GG
SfuI          743  TTCGAA               TT^CGAA
SmaI          796  CCCGGG               CCC^GGG
SphI          411  GCATGC               GCATG^C
Sse8387I      703  CCTGCAGG             CCTGCA^GG
SspBI        1713  TGTACA               T^GTACA
SspI         1807  AATATT               AAT^ATT
StuI           65  AGGCCT               AGG^CCT
XbaI          282  TCTAGA               T^CTAGA
XmaCI         796  CCCGGG               C^CCGGG
XmaI          796  CCCGGG               C^CCGGG
XmnI          563  GAACAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI          167  GGATC                GGATC(4/5)
              972  GGATC               
AspHI         719  GTGCAC               G[AT]GC[AT]^C
              961  GAGCTC              
AvaII         202  GGTCC                G^G[AT]CC
             1116  GGACC               
BanI          327  GGTGCC               G^G[CT][GA]CC
              737  GGTACC              
BciVI         553  GTATCC               GTATCC(6/5)
              729  GTATCC              
BcnI          796  CCCGG                CC^[GC]GG
              797  CCGGG               
BsiHKAI       719  GTGCAC               G[AT]GC[AT]^C
              961  GAGCTC              
BstYI         844  AGATCT               [GA]^GATC[CT]
              972  GGATCT              
Cac8I         411  GCATGC               GC[ACGT]^[ACGT]GC
             1443  GCAAGC              
Cfr13I        202  GGTCC                G^G[ACGT]CC
             1116  GGACC               
EcoT14I      1119  CCAAGG               C^C[AT][AT]GG
             1421  CCAAGG              
HaeIII         66  GGCC                 GG^CC
             1008  GGCC                
HapII         797  CCGG                 C^CGG
              850  CCGG                
HpaII         797  CCGG                 C^CGG
              850  CCGG                
MflI          844  AGATCT               [GA]^GATC[CT]
              972  GGATCT              
MspI          797  CCGG                 C^CGG
              850  CCGG                
NciI          796  CCCGG                CC^[GC]GG
              797  CCGGG               
PflMI          13  CCACAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1764  CCATGCGGTGG         
PleI          187  GAGTC                GAGTC(4/5)
             1197  GAGTC               
PstI          307  CTGCAG               CTGCA^G
              704  CTGCAG              
Sau96I        202  GGTCC                G^G[ACGT]CC
             1116  GGACC               
StyI         1119  CCAAGG               C^C[AT][AT]GG
             1421  CCAAGG              
TaqI          744  TCGA                 T^CGA
              773  TCGA                
TthHB8I       744  TCGA                 T^CGA
              773  TCGA                
Van91I         13  CCACAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1764  CCATGCGGTGG         
XhoII         844  AGATCT               [GA]^GATC[CT]
              972  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwNI       ApoI        BbvI        BfaI        BmyI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
Bsp1286I    BspLU11I    BspMI       BsrI        BssKI       
BstF5I      BstNI       DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HincII      HindII      HinfI       
HpaI        HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      ScrFI       SfaNI       
SfcI        SmlI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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