Restriction map for pF1KA0589
 (Linear) (Six-base) MAPSORT of: KIAA0589.seq  Check: 4395  from: 1  to: 2004



KIAA0589.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1722    1875    2004 
   Size:       1722     153     129

  Fragments arranged by size: 

                1722     153     129
 
AatII G_ACGT'C

Cuts at:      0    1630    2004 
   Size:       1630     374
 
Acc65I G'GTAC_C

Cuts at:      0      12    2004 
   Size:         12    1992
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     867    1369    2004 
   Size:        256     611     502     635

  Fragments arranged by size: 

                 635     611     502     256
 
AfeI AGC'GCT

Cuts at:      0      33    1300    2004 
   Size:         33    1267     704

  Fragments arranged by size: 

                1267     704      33
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1266    2004 
   Size:       1266     738
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     571     603    2004 
   Size:        571      32    1401

  Fragments arranged by size: 

                1401     571      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     600    2004 
   Size:        600    1404
 
ApaI G_GGCC'C

Cuts at:      0     154    1412    1482    2004 
   Size:        154    1258      70     522

  Fragments arranged by size: 

                1258     522     154      70
 
ApaLI G'TGCA_C

Cuts at:      0     967    2004 
   Size:        967    1037
 
AscI GG'CGCG_CC

Cuts at:      0     783    2004 
   Size:        783    1221
 
AvaI C'yCGr_G

Cuts at:      0     507     853    2004 
   Size:        507     346    1151

  Fragments arranged by size: 

                1151     507     346
 
BamHI G'GATC_C

Cuts at:      0     446    1136    2004 
   Size:        446     690     868

  Fragments arranged by size: 

                 868     690     446
 
BanI G'GyrC_C

Cuts at:      0      12     241     511    1009    1090    1807    2004 
   Size:         12     229     270     498      81     717     197

  Fragments arranged by size: 

                 717     498     270     229     197      81      12
 
BanII G_rGCy'C

Cuts at:      0     154     286     440     769     863    1412    1482    2004 
   Size:        154     132     154     329      94     549      70     522

  Fragments arranged by size: 

                 549     522     329     154     154     132      94      70
            
 
BbeI G_GCGC'C

Cuts at:      0     515    1013    1094    2004 
   Size:        515     498      81     910

  Fragments arranged by size: 

                 910     515     498      81
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     223    2004 
   Size:        223    1781
 
BbvCI CC'TCA_GC

Cuts at:      0      35    2004 
   Size:         35    1969
 
BglI GCCn_nnn'nGGC

Cuts at:      0     858    1426    1843    1876    1978    2004 
   Size:        858     568     417      33     102      26

  Fragments arranged by size: 

                 858     568     417     102      33      26
 
Bme1580I G_kGCm'C

Cuts at:      0      53     154     971    1412    1482    2004 
   Size:         53     101     817     441      70     522

  Fragments arranged by size: 

                 817     522     441     101      70      53
 
BmgBI CAC'GTC

Cuts at:      0     303    2004 
   Size:        303    1701
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1455    2004 
   Size:       1455     549
 
BmtI G_CTAG'C

Cuts at:      0    1393    2004 
   Size:       1393     611
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28    1246    1519    2004 
   Size:         28    1218     273     485

  Fragments arranged by size: 

                1218     485     273      28
 
Bpu10I CC'TnA_GC

Cuts at:      0      35     280    1607    2004 
   Size:         35     245    1327     397

  Fragments arranged by size: 

                1327     397     245      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     733     745    2004 
   Size:        733      12    1259

  Fragments arranged by size: 

                1259     733      12
 
BsaAI yAC'GTr

Cuts at:      0     324    2004 
   Size:        324    1680
 
BsaHI Gr'CG_yC

Cuts at:      0     512     872    1010    1091    1627    2004 
   Size:        512     360     138      81     536     377

  Fragments arranged by size: 

                 536     512     377     360     138      81
 
BsaWI w'CCGG_w

Cuts at:      0      15    2004 
   Size:         15    1989
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      49      79     268     298     454     484     571     601 
   Size:         49      30     189      30     156      30      87      30

Cuts at:    601    1447    1477    1729    1759    2004 
   Size:        846      30     252      30     245

  Fragments arranged by size: 

                 846     252     245     189     156      87      49      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     216    1332    1468    1705    1789    1870    1903    1947 
   Size:        216    1116     136     237      84      81      33      44

Cuts at:   1947    2004 
   Size:         57

  Fragments arranged by size: 

                1116     237     216     136      84      81      57      44
                  33
 
BseYI C'CCAG_C

Cuts at:      0     252     346     960    1282    1404    1937    2004 
   Size:        252      94     614     322     122     533      67

  Fragments arranged by size: 

                 614     533     322     252     122      94      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697    1159    1515    1750    1767    2004 
   Size:        697     462     356     235      17     237

  Fragments arranged by size: 

                 697     462     356     237     235      17
 
BsiEI CG_ry'CG

Cuts at:      0    1155    1805    2004 
   Size:       1155     650     199

  Fragments arranged by size: 

                1155     650     199
 
BsiHKAI G_wGCw'C

Cuts at:      0     286     308     440     971    1021    1232    2004 
   Size:        286      22     132     531      50     211     772

  Fragments arranged by size: 

                 772     531     286     211     132      50      22
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1618    2004 
   Size:       1618     386
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     154     286     308     440     769     863     971 
   Size:         53     101     132      22     132     329      94     108

Cuts at:    971    1021    1232    1412    1482    2004 
   Size:         50     211     180      70     522

  Fragments arranged by size: 

                 522     329     211     180     132     132     108     101
                  94      70      53      50      22
 
BspHI T'CATG_A

Cuts at:      0     710    1313    2004 
   Size:        710     603     691

  Fragments arranged by size: 

                 710     691     603
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      82    1478    1722    1875    2004 
   Size:         82    1396     244     153     129

  Fragments arranged by size: 

                1396     244     153     129      82
 
BsrBI CCG'CTC

Cuts at:      0      80     996    1164    1449    1611    1953    2004 
   Size:         80     916     168     285     162     342      51

  Fragments arranged by size: 

                 916     342     285     168     162      80      51
 
BsrDI GCAATG_nn'

Cuts at:      0     486    1568    2004 
   Size:        486    1082     436

  Fragments arranged by size: 

                1082     486     436
 
BsrFI r'CCGG_y

Cuts at:      0    1511    1804    2004 
   Size:       1511     293     200

  Fragments arranged by size: 

                1511     293     200
 
BssHII G'CGCG_C

Cuts at:      0     783    1096    2004 
   Size:        783     313     908

  Fragments arranged by size: 

                 908     783     313
 
BssSI C'ACGA_G

Cuts at:      0     985    2004 
   Size:        985    1019
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     600     849    2004 
   Size:        600     249    1155

  Fragments arranged by size: 

                1155     600     249
 
BstBI TT'CG_AA

Cuts at:      0    1547    2004 
   Size:       1547     457
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     252    1071    2004 
   Size:        252     819     933

  Fragments arranged by size: 

                 933     819     252
 
BstYI r'GATC_y

Cuts at:      0     446    1136    1436    1710    2004 
   Size:        446     690     300     274     294

  Fragments arranged by size: 

                 690     446     300     294     274
 
BtgI C'CryG_G

Cuts at:      0     159     269     637    1064    1070    1155    1639    1700 
   Size:        159     110     368     427       6      85     484      61

Cuts at:   1700    2004 
   Size:        304

  Fragments arranged by size: 

                 484     427     368     304     159     110      85      61
                   6
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     919    2004 
   Size:        919    1085
 
EaeI y'GGCC_r

Cuts at:      0     156     451    1067    1152    1427    1802    2004 
   Size:        156     295     616      85     275     375     202

  Fragments arranged by size: 

                 616     375     295     275     202     156      85
 
EagI C'GGCC_G

Cuts at:      0    1152    1802    2004 
   Size:       1152     650     202

  Fragments arranged by size: 

                1152     650     202
 
EarI CTCTTCn'nnn_

Cuts at:      0     532     802    1700    2004 
   Size:        532     270     898     304

  Fragments arranged by size: 

                 898     532     304     270
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      40    1394    2004 
   Size:         40    1354     610

  Fragments arranged by size: 

                1354     610      40
 
EcoICRI GAG'CTC

Cuts at:      0     284     438    2004 
   Size:        284     154    1566

  Fragments arranged by size: 

                1566     284     154
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     256     867    1246    1369    1519    2004 
   Size:         28     228     611     379     123     150     485

  Fragments arranged by size: 

                 611     485     379     228     150     123      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     151     828    1242    1408    1478    1971    2004 
   Size:        151     677     414     166      70     493      33

  Fragments arranged by size: 

                 677     493     414     166     151      70      33
 
FseI GG_CCGG'CC

Cuts at:      0    1515    2004 
   Size:       1515     489
 
HaeII r_GCGC'y

Cuts at:      0      35     515     890    1013    1057    1094    1302    1967 
   Size:         35     480     375     123      44      37     208     665

Cuts at:   1967    2004 
   Size:         37

  Fragments arranged by size: 

                 665     480     375     208     123      44      37      37
                  35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     147    1252    1284    2004 
   Size:        115      32    1105      32     720

  Fragments arranged by size: 

                1105     720     115      32      32
 
KasI G'GCGC_C

Cuts at:      0     511    1009    1090    2004 
   Size:        511     498      81     914

  Fragments arranged by size: 

                 914     511     498      81
 
KpnI G_GTAC'C

Cuts at:      0      16    2004 
   Size:         16    1988
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     527    1610    2004 
   Size:        527    1083     394

  Fragments arranged by size: 

                1083     527     394
 
MscI TGG'CCA

Cuts at:      0     158    2004 
   Size:        158    1846
 
MslI CAynn'nnrTG

Cuts at:      0     182    1069    2004 
   Size:        182     887     935

  Fragments arranged by size: 

                 935     887     182
 
MspA1I CmG'CkG

Cuts at:      0     252     489     639     909    1144    1157    1404    1417 
   Size:        252     237     150     270     235      13     247      13

Cuts at:   1417    1641    1702    2004 
   Size:        224      61     302

  Fragments arranged by size: 

                 302     270     252     247     237     235     224     150
                  61      13      13
 
NaeI GCC'GGC

Cuts at:      0    1513    2004 
   Size:       1513     491
 
NarI GG'CG_CC

Cuts at:      0     512    1010    1091    2004 
   Size:        512     498      81     913

  Fragments arranged by size: 

                 913     512     498      81
 
NcoI C'CATG_G

Cuts at:      0     159    1070    2004 
   Size:        159     911     934

  Fragments arranged by size: 

                 934     911     159
 
NgoMIV G'CCGG_C

Cuts at:      0    1511    2004 
   Size:       1511     493
 
NheI G'CTAG_C

Cuts at:      0    1389    2004 
   Size:       1389     615
 
NspI r_CATG'y

Cuts at:      0     852    2004 
   Size:        852    1152
 
PfoI T'CCnGG_A

Cuts at:      0     383     431     923    2004 
   Size:        383      48     492    1081

  Fragments arranged by size: 

                1081     492     383      48
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     571     603    2004 
   Size:        571      32    1401

  Fragments arranged by size: 

                1401     571      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     828    2004 
   Size:        828    1176
 
PspOMI G'GGCC_C

Cuts at:      0     150    1408    1478    2004 
   Size:        150    1258      70     526

  Fragments arranged by size: 

                1258     526     150      70
 
PstI C_TGCA'G

Cuts at:      0     596     908    1202    1718    2004 
   Size:        596     312     294     516     286

  Fragments arranged by size: 

                 596     516     312     294     286
 
PvuII CAG'CTG

Cuts at:      0     252    2004 
   Size:        252    1752
 
SacI G_AGCT'C

Cuts at:      0     286     440    2004 
   Size:        286     154    1564

  Fragments arranged by size: 

                1564     286     154
 
SacII CC_GC'GG

Cuts at:      0     640    1158    1642    1703    2004 
   Size:        640     518     484      61     301

  Fragments arranged by size: 

                 640     518     484     301      61
 
SapI GCTCTTCn'nnn_

Cuts at:      0     802    2004 
   Size:        802    1202
 
SfcI C'TryA_G

Cuts at:      0     592     904     948    1198    1206    1559    1714    1959 
   Size:        592     312      44     250       8     353     155     245

Cuts at:   1959    2004 
   Size:         45

  Fragments arranged by size: 

                 592     353     312     250     245     155      45      44
                   8
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1978    2004 
   Size:       1978      26
 
SfoI GGC'GCC

Cuts at:      0     513    1011    1092    2004 
   Size:        513     498      81     912

  Fragments arranged by size: 

                 912     513     498      81
 
SmaI CCC'GGG

Cuts at:      0     509    2004 
   Size:        509    1495
 
SmlI C'TyrA_G

Cuts at:      0     748     760    2004 
   Size:        748      12    1244

  Fragments arranged by size: 

                1244     748      12
 
StuI AGG'CCT

Cuts at:      0    1846    1879    2004 
   Size:       1846      33     125

  Fragments arranged by size: 

                1846     125      33
 
StyI C'CwwG_G

Cuts at:      0     159    1070    2004 
   Size:        159     911     934

  Fragments arranged by size: 

                 934     911     159
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1929    2004 
   Size:       1929      75
 
TatI w'GTAC_w

Cuts at:      0     467     668    1943    2004 
   Size:        467     201    1275      61

  Fragments arranged by size: 

                1275     467     201      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      49      81     268     300    1729    1761    2004 
   Size:         49      32     187      32    1429      32     243

  Fragments arranged by size: 

                1429     243     187      49      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1581    2004 
   Size:       1581     423
 
XmaI C'CCGG_G

Cuts at:      0     507    2004 
   Size:        507    1497
 
XmnI GAAnn'nnTTC

Cuts at:      0     811    2004 
   Size:        811    1193
 
ZraI GAC'GTC

Cuts at:      0    1628    2004 
   Size:       1628     376
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI     AhdI     AloI    AlwNI
     ApaI    ApaLI     AscI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BglI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
     BtgI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FseI    HaeII    Hin4I     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI
     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII
     SapI     SfcI     SfiI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII   AflIII     AgeI     AleI     AlfI     ApoI
     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BplI     BsaI    BsaBI    BsiWI     BsmI    BspEI
    BsrGI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII    EcoNI    EcoRI    EcoRV     FalI     FspI    FspAI
   HincII  HindIII     HpaI     MfeI     MluI     NdeI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SbfI
     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XcmI     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com