Restriction map for
pF1KA0584
(Linear) (Six-base) MAPSORT of: KIAA0584.seq Check: 4622 from: 1 to: 1878
KIAA0584.orf
With 182 enzymes: SgfI *
October 24, 2006 15:07 ..
Acc65I G'GTAC_C
Cuts at: 0 1700 1878
Size: 1700 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1177 1878
Size: 907 270 701
Fragments arranged by size:
907 701 270
AlwNI CAG_nnn'CTG
Cuts at: 0 271 903 1527 1878
Size: 271 632 624 351
Fragments arranged by size:
632 624 351 271
AvaI C'yCGr_G
Cuts at: 0 584 1878
Size: 584 1294
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1692 1725 1878
Size: 1692 33 153
Fragments arranged by size:
1692 153 33
BanI G'GyrC_C
Cuts at: 0 1700 1878
Size: 1700 178
BanII G_rGCy'C
Cuts at: 0 157 1878
Size: 157 1721
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1297 1313 1878
Size: 917 380 16 565
Fragments arranged by size:
917 565 380 16
BbvCI CC'TCA_GC
Cuts at: 0 51 1878
Size: 51 1827
BclI T'GATC_A
Cuts at: 0 273 1878
Size: 273 1605
BglI GCCn_nnn'nGGC
Cuts at: 0 262 1878
Size: 262 1616
BlpI GC'TnA_GC
Cuts at: 0 1376 1878
Size: 1376 502
Bme1580I G_kGCm'C
Cuts at: 0 1448 1878
Size: 1448 430
BmrI ACTGGGnnnn_n'
Cuts at: 0 972 1395 1767 1878
Size: 972 423 372 111
Fragments arranged by size:
972 423 372 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 117 1878
Size: 117 1761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 598 801 1191 1878
Size: 244 354 203 390 687
Fragments arranged by size:
687 390 354 244 203
Bpu10I CC'TnA_GC
Cuts at: 0 51 1878
Size: 51 1827
BsaI GGTCTCn'nnnn_
Cuts at: 0 1711 1878
Size: 1711 167
BsaBI GATnn'nnATC
Cuts at: 0 256 1878
Size: 256 1622
BsaWI w'CCGG_w
Cuts at: 0 1070 1878
Size: 1070 808
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 39 133 1878
Size: 34 5 94 1745
Fragments arranged by size:
1745 94 34 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1878
Size: 955 923
BsiHKAI G_wGCw'C
Cuts at: 0 167 863 1521 1878
Size: 167 696 658 357
Fragments arranged by size:
696 658 357 167
Bsp1286I G_dGCh'C
Cuts at: 0 157 167 863 1448 1521 1878
Size: 157 10 696 585 73 357
Fragments arranged by size:
696 585 357 157 73 10
BspHI T'CATG_A
Cuts at: 0 1034 1878
Size: 1034 844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 881 1878
Size: 881 997
BsrBI CCG'CTC
Cuts at: 0 96 228 1878
Size: 96 132 1650
Fragments arranged by size:
1650 132 96
BsrDI GCAATG_nn'
Cuts at: 0 953 1815 1878
Size: 953 862 63
Fragments arranged by size:
953 862 63
BsrFI r'CCGG_y
Cuts at: 0 123 1878
Size: 123 1755
BsrGI T'GTAC_A
Cuts at: 0 324 1586 1878
Size: 324 1262 292
Fragments arranged by size:
1262 324 292
BssHII G'CGCG_C
Cuts at: 0 7 79 1878
Size: 7 72 1799
Fragments arranged by size:
1799 72 7
BssSI C'ACGA_G
Cuts at: 0 166 1878
Size: 166 1712
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 1878
Size: 201 1677
BstBI TT'CG_AA
Cuts at: 0 456 1878
Size: 456 1422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 404 1878
Size: 404 1474
BstYI r'GATC_y
Cuts at: 0 1194 1878
Size: 1194 684
Bsu36I CC'TnA_GG
Cuts at: 0 623 727 1878
Size: 623 104 1151
Fragments arranged by size:
1151 623 104
BtgI C'CryG_G
Cuts at: 0 158 698 1878
Size: 158 540 1180
Fragments arranged by size:
1180 540 158
BtsI GCAGTG_nn'
Cuts at: 0 901 1448 1878
Size: 901 547 430
Fragments arranged by size:
901 547 430
DraIII CAC_nnn'GTG
Cuts at: 0 282 345 1878
Size: 282 63 1533
Fragments arranged by size:
1533 282 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 802 1878
Size: 802 1076
EaeI y'GGCC_r
Cuts at: 0 254 406 1748 1878
Size: 254 152 1342 130
Fragments arranged by size:
1342 254 152 130
EarI CTCTTCn'nnn_
Cuts at: 0 667 1405 1878
Size: 667 738 473
Fragments arranged by size:
738 667 473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 598 801 907 1177 1191 1878
Size: 244 354 203 106 270 14 687
Fragments arranged by size:
687 354 270 244 203 106 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 730 1878
Size: 730 1148
EcoO109I rG'GnC_Cy
Cuts at: 0 588 641 1635 1827 1878
Size: 588 53 994 192 51
Fragments arranged by size:
994 588 192 53 51
FspI TGC'GCA
Cuts at: 0 1081 1878
Size: 1081 797
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 321 353 519 551 942 974 1878
Size: 321 32 166 32 391 32 904
Fragments arranged by size:
904 391 321 166 32 32 32
HincII GTy'rAC
Cuts at: 0 504 1878
Size: 504 1374
KpnI G_GTAC'C
Cuts at: 0 1704 1878
Size: 1704 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 1878
Size: 1512 366
MscI TGG'CCA
Cuts at: 0 256 408 1750 1878
Size: 256 152 1342 128
Fragments arranged by size:
1342 256 152 128
MslI CAynn'nnrTG
Cuts at: 0 838 931 1878
Size: 838 93 947
Fragments arranged by size:
947 838 93
MspA1I CmG'CkG
Cuts at: 0 1155 1380 1878
Size: 1155 225 498
Fragments arranged by size:
1155 498 225
NcoI C'CATG_G
Cuts at: 0 698 1878
Size: 698 1180
NspI r_CATG'y
Cuts at: 0 1774 1878
Size: 1774 104
PasI CC'CwG_GG
Cuts at: 0 1491 1878
Size: 1491 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 1730 1757 1878
Size: 1730 27 121
Fragments arranged by size:
1730 121 27
PfoI T'CCnGG_A
Cuts at: 0 134 1783 1878
Size: 134 1649 95
Fragments arranged by size:
1649 134 95
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1649 1681 1878
Size: 1649 32 197
Fragments arranged by size:
1649 197 32
PpuMI rG'GwC_Cy
Cuts at: 0 641 1635 1878
Size: 641 994 243
Fragments arranged by size:
994 641 243
PstI C_TGCA'G
Cuts at: 0 152 908 1656 1878
Size: 152 756 748 222
Fragments arranged by size:
756 748 222 152
PvuII CAG'CTG
Cuts at: 0 1155 1380 1878
Size: 1155 225 498
Fragments arranged by size:
1155 498 225
SexAI A'CCwGG_T
Cuts at: 0 1460 1878
Size: 1460 418
SfcI C'TryA_G
Cuts at: 0 148 904 1652 1803 1878
Size: 148 756 748 151 75
Fragments arranged by size:
756 748 151 148 75
SspI AAT'ATT
Cuts at: 0 302 1170 1878
Size: 302 868 708
Fragments arranged by size:
868 708 302
StuI AGG'CCT
Cuts at: 0 357 739 1212 1878
Size: 357 382 473 666
Fragments arranged by size:
666 473 382 357
StyI C'CwwG_G
Cuts at: 0 698 1878
Size: 698 1180
TatI w'GTAC_w
Cuts at: 0 324 1586 1610 1878
Size: 324 1262 24 268
Fragments arranged by size:
1262 324 268 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 249 1878
Size: 165 84 1629
Fragments arranged by size:
1629 165 84
XmnI GAAnn'nnTTC
Cuts at: 0 1645 1878
Size: 1645 233
Enzymes that do cut:
Acc65I AcuI AlwNI AvaI BaeI BanI BanII BbsI
BbvCI BclI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsaBI BsaWI BseRI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I FspI Hin4I
HincII KpnI MmeI MscI MslI MspA1I NcoI NspI
PasI PflMI PfoI PpiI PpuMI PstI PvuII SexAI
SfcI SspI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BbeI BcgI BciVI BfrBI
BglII BmgBI BmtI BpuEI BsaAI BsaHI BsaXI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BstEII BstZ17I BtgZI
ClaI CspCI DraI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspAI HaeII HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI ZraI