Restriction map for pF1KA0560
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        AhdI        Aor51HI     AscI        AspEI       
AvrII       BbeI        BbrPI       BbvCI       BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BseAI       BsiEI       BsmBI       
BspDI       BspEI       BsrBI       BssHII      BssSI       
CelII       ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      FauI        
FseI        Hin1I       HpaI        KasI        KspI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SwaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       2046  GGTACC               G^GTACC
Alw44I       2096  GTGCAC               G^TGCAC
ApaI         2466  GGGCCC               GGGCC^C
ApaLI        2096  GTGCAC               G^TGCAC
Asp718I      2046  GGTACC               G^GTACC
AspHI        2096  GTGCAC               G[AT]GC[AT]^C
AspI         3142  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII          12  TGCGCA               TGC^GCA
BanI         2046  GGTACC               G^G[CT][GA]CC
BclI          450  TGATCA               T^GATCA
BsaBI         880  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1733  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      2096  GTGCAC               G[AT]GC[AT]^C
BsiWI        1698  CGTACG               C^GTACG
BsmI         2438  GAATGC               GAATGC(1/-1)
BsrFI        4401  ACCGGC               [GA]^CCGG[CT]
Bst1107I     4093  GTATAC               GTA^TAC
BstEII       3652  GGTTACC              G^GT[ACGT]ACC
BstPI        3652  GGTTACC              G^GT[ACGT]ACC
BstXI        3833  CCAGGGCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      4093  GTATAC               GTA^TAC
Cfr10I       4401  ACCGGC               [GA]^CCGG[CT]
DraIII       1132  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1480  CCATGG               C^C[GA][CT]GG
EcoNI        2473  CCTGGTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3652  GGTTACC              G^GT[ACGT]ACC
EcoRI        3028  GAATTC               G^AATTC
EcoRV        2065  GATATC               GAT^ATC
EcoT22I      4108  ATGCAT               ATGCA^T
FbaI          450  TGATCA               T^GATCA
FspI           12  TGCGCA               TGC^GCA
HgaI         2631  GCGTC                GACGC(5/10)
HindIII      3423  AAGCTT               A^AGCTT
KpnI         2046  GGTACC               GGTAC^C
MamI          880  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         1480  CCATGG               C^CATGG
NruI         3711  TCGCGA               TCG^CGA
NsiI         4108  ATGCAT               ATGCA^T
PflFI        3142  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         2792  CTGCAG               CTGCA^G
SphI         1796  GCATGC               GCATG^C
SspI         1309  AATATT               AAT^ATT
StuI          263  AGGCCT               AGG^CCT
Tth111I      3142  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI          475  GTAGAC               GT^[AC][GT]AC
             4093  GTATAC              
AseI          937  ATTAAT               AT^TAAT
             3538  ATTAAT              
AsnI          937  ATTAAT               AT^TAAT
             3538  ATTAAT              
AvaI         1082  CTCGGG               C^[CT]CG[GA]G
             1598  CCCGAG              
BamHI         621  GGATCC               G^GATCC
             1887  GGATCC              
BcgI         2098  GCACATTATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3702  GCATCTTATTCG        
BciVI        1278  GTATCC               GTATCC(6/5)
             3919  GTATCC              
BglII        2953  AGATCT               A^GATCT
             3067  AGATCT              
BsoBI        1082  CTCGGG               C^[CT]CG[GA]G
             1598  CCCGAG              
Bsp1407I     3635  TGTACA               T^GTACA
             4103  TGTACA              
BspLU11I     3638  ACATGT               A^CATGT
             4100  ACATGT              
BsrDI         454  CATTGC               GCAATG(2/0)
             2870  CATTGC              
BsrGI        3635  TGTACA               T^GTACA
             4103  TGTACA              
BstAPI          4  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3480  GCAGCTCTTGC         
BstBI        2106  TTCGAA               TT^CGAA
             3050  TTCGAA              
EcoT14I      1091  CCTTGG               C^C[AT][AT]GG
             1480  CCATGG              
HaeII         800  GGCGCT               [GA]GCGC^[CT]
             2618  AGCGCC              
HapII        1734  CCGG                 C^CGG
             4402  CCGG                
HincII       3391  GTTGAC               GT[CT]^[GA]AC
             3794  GTCAAC              
HindII       3391  GTTGAC               GT[CT]^[GA]AC
             3794  GTCAAC              
HpaII        1734  CCGG                 C^CGG
             4402  CCGG                
MspA1I        535  CAGCTG               C[AC]G^C[GT]G
             4272  CAGCTG              
MspI         1734  CCGG                 C^CGG
             4402  CCGG                
NspV         2106  TTCGAA               TT^CGAA
             3050  TTCGAA              
PpuMI        2084  GGGACCC              [GA]G^G[AT]CC[CT]
             2361  GGGTCCT             
PshBI         937  ATTAAT               AT^TAAT
             3538  ATTAAT              
PvuII         535  CAGCTG               CAG^CTG
             4272  CAGCTG              
SapI         2650  GAAGAGC              GCTCTTC(1/4)
             1351  GCTCTTC             
ScaI         1325  AGTACT               AGT^ACT
             3332  AGTACT              
SfuI         2106  TTCGAA               TT^CGAA
             3050  TTCGAA              
SmlI         3022  CTTGAG               C^T[CT][GA]AG
             3404  CTCAAG              
SspBI        3635  TGTACA               T^GTACA
             4103  TGTACA              
StyI         1091  CCTTGG               C^C[AT][AT]GG
             1480  CCATGG              
XcmI           20  CCAAGAAGATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4370  CCACCCCTACTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AvaII       BalI        BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XhoII       XmnI        
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