Restriction map for pF1KA0528
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AgeI        
AhdI        AocI        Aor51HI     AscI        Asp718I     
AspEI       AspI        AviII       BalI        BamHI       
BbrPI       BbvCI       BcgI        BclI        BglI        
BsaAI       BsaI        BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BspHI       BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cfr10I      CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     FauI        FbaI        FseI        FspI        
HpaI        KpnI        KspI        MluI        MluNI       
MscI        MvnI        NaeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        Tth111I     
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccIII       2576  TCCGGA               T^CCGGA
AciI         1297  GCGG                 CCGC(-3/-1)
AcyI           35  GGCGCC               G[GA]^CG[CT]C
AflII         130  CTTAAG               C^TTAAG
Alw44I       1106  GTGCAC               G^TGCAC
AlwNI        1295  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          985  GGGCCC               GGGCC^C
ApaLI        1106  GTGCAC               G^TGCAC
AseI         2349  ATTAAT               AT^TAAT
AsnI         2349  ATTAAT               AT^TAAT
Asp700I      2347  GAATTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1160  CTCGAG               C^[CT]CG[GA]G
AvrII        1942  CCTAGG               C^CTAGG
BbeI           35  GGCGCC               GGCGC^C
BfrI          130  CTTAAG               C^TTAAG
BglII        1966  AGATCT               A^GATCT
BlnI         1942  CCTAGG               C^CTAGG
BlpI         1730  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1730  GCTTAGC              GC^T[ACGT]AGC
BsaHI          35  GGCGCC               G[GA]^CG[CT]C
BsaWI        2576  TCCGGA               [AT]^CCGG[AT]
BseAI        2576  TCCGGA               T^CCGGA
BsoBI        1160  CTCGAG               C^[CT]CG[GA]G
BspDI         404  ATCGAT               AT^CGAT
BspEI        2576  TCCGGA               T^CCGGA
BspLU11I     2687  ACATGT               A^CATGT
BstAPI        244  GCAAATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         708  GCGAGC               GC[ACGT]^[ACGT]GC
CelII        1730  GCTTAGC              GC^T[ACGT]AGC
CfoI           36  GCGC                 GCG^C
ClaI          404  ATCGAT               AT^CGAT
EaeI           29  TGGCCG               [CT]^GGCC[GA]
EcoNI        2098  CCTCCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1905  GATATC               GAT^ATC
EcoT22I      1166  ATGCAT               ATGCA^T
HaeII          35  GGCGCC               [GA]GCGC^[CT]
HhaI           36  GCGC                 GCG^C
Hin1I          35  GGCGCC               G[GA]^CG[CT]C
HinP1I         36  GCGC                 G^CGC
HincII        902  GTCGAC               GT[CT]^[GA]AC
HindII        902  GTCGAC               GT[CT]^[GA]AC
HindIII      1120  AAGCTT               A^AGCTT
KasI           35  GGCGCC               G^GCGCC
MfeI         1862  CAATTG               C^AATTG
MroI         2576  TCCGGA               T^CCGGA
MslI         1743  CACAGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1295  CAGCGG               C[AC]G^C[GT]G
MunI         1862  CAATTG               C^AATTG
NarI           35  GGCGCC               GG^CGCC
NcoI         2060  CCATGG               C^CATGG
NsiI         1166  ATGCAT               ATGCA^T
PaeR7I       1160  CTCGAG               C^TCGAG
PflMI        1768  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         843  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2349  ATTAAT               AT^TAAT
SacI         2783  GAGCTC               GAGCT^C
SalI          902  GTCGAC               G^TCGAC
ScaI          630  AGTACT               AGT^ACT
SfoI           35  GGCGCC               GGC^GCC
SwaI          398  ATTTAAAT             ATTT^AAAT
Van91I       1768  CCAACTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1160  CTCGAG               C^TCGAG
XmnI         2347  GAATTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          382  GTAGAC               GT^[AC][GT]AC
              902  GTCGAC              
AspHI        1106  GTGCAC               G[AT]GC[AT]^C
             2783  GAGCTC              
BanI           35  GGCGCC               G^G[CT][GA]CC
             1330  GGCACC              
BanII         985  GGGCCC               G[GA]GC[CT]^C
             2783  GAGCTC              
BciVI         350  GTATCC               GTATCC(6/5)
             1133  GTATCC              
BcnI           32  CCGGG                CC^[GC]GG
             2137  CCCGG               
BseRI        2096  CTCCTC               GAGGAG(10/8)
             2729  GAGGAG              
BsiHKAI      1106  GTGCAC               G[AT]GC[AT]^C
             2783  GAGCTC              
Bsp1407I      266  TGTACA               T^GTACA
             1083  TGTACA              
BsrDI        2435  CATTGC               GCAATG(2/0)
             2773  GCAATG              
BsrGI         266  TGTACA               T^GTACA
             1083  TGTACA              
BstYI         816  AGATCC               [GA]^GATC[CT]
             1966  AGATCT              
DraIII       1379  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2594  CACCTTGTG           
EcoRI         542  GAATTC               G^AATTC
             1694  GAATTC              
HgaI         2899  GACGC                GACGC(5/10)
             1735  GCGTC               
MflI          816  AGATCC               [GA]^GATC[CT]
             1966  AGATCT              
NciI           32  CCGGG                CC^[GC]GG
             2137  CCCGG               
SspBI         266  TGTACA               T^GTACA
             1083  TGTACA              
XcmI          520  CCAGAATATCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              944  CCAAAACTGGAATGG     
XhoII         816  AGATCC               [GA]^GATC[CT]
             1966  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwI        
ApoI        AvaII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaBI       BsaJI       
BsiYI       BslI        BsmAI       BsmI        Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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