Restriction map for pF1KA0528
 (Linear) (Six-base) MAPSORT of: KIAA0528.seq  Check: 3493  from: 1  to: 3000



KIAA0528.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AccI GT'mk_AC

Cuts at:      0     383     903    3000 
   Size:        383     520    2097

  Fragments arranged by size: 

                2097     520     383
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34    1931    2087    2162    2360    2783    3000 
   Size:         34    1897     156      75     198     423     217

  Fragments arranged by size: 

                1897     423     217     198     156      75      34
 
AflII C'TTAA_G

Cuts at:      0     130    3000 
   Size:        130    2870
 
AflIII A'CryG_T

Cuts at:      0    1086    1378    2687    3000 
   Size:       1086     292    1309     313

  Fragments arranged by size: 

                1309    1086     313     292
 
AleI CACnn'nnGTG

Cuts at:      0    1747    3000 
   Size:       1747    1253
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1300    3000 
   Size:       1300    1700
 
ApaI G_GGCC'C

Cuts at:      0     989    3000 
   Size:        989    2011
 
ApaLI G'TGCA_C

Cuts at:      0    1106    3000 
   Size:       1106    1894
 
ApoI r'AATT_y

Cuts at:      0      94     169     433     542    1039    1368    1694    2256 
   Size:         94      75     264     109     497     329     326     562

Cuts at:   2256    2319    2571    2604    3000 
   Size:         63     252      33     396

  Fragments arranged by size: 

                 562     497     396     329     326     264     252     109
                  94      75      63      33
 
AseI AT'TA_AT

Cuts at:      0    2350    3000 
   Size:       2350     650
 
AvaI C'yCGr_G

Cuts at:      0    1160    3000 
   Size:       1160    1840
 
AvrII C'CTAG_G

Cuts at:      0    1942    3000 
   Size:       1942    1058
 
BanI G'GyrC_C

Cuts at:      0      35    1330    3000 
   Size:         35    1295    1670

  Fragments arranged by size: 

                1670    1295      35
 
BanII G_rGCy'C

Cuts at:      0     989    2787    3000 
   Size:        989    1798     213

  Fragments arranged by size: 

                1798     989     213
 
BbeI G_GCGC'C

Cuts at:      0      39    3000 
   Size:         39    2961
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     194     521    1804    2835    3000 
   Size:        194     327    1283    1031     165

  Fragments arranged by size: 

                1283    1031     327     194     165
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     361    1144    3000 
   Size:        361     783    1856

  Fragments arranged by size: 

                1856     783     361
 
BfrBI ATG'CAT

Cuts at:      0    1168    3000 
   Size:       1168    1832
 
BglII A'GATC_T

Cuts at:      0    1966    3000 
   Size:       1966    1034
 
BlpI GC'TnA_GC

Cuts at:      0    1731    3000 
   Size:       1731    1269
 
Bme1580I G_kGCm'C

Cuts at:      0     989    1110    3000 
   Size:        989     121    1890

  Fragments arranged by size: 

                1890     989     121
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1883    2672    3000 
   Size:        703    1180     789     328

  Fragments arranged by size: 

                1180     789     703     328
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1938    2248    3000 
   Size:       1938     310     752

  Fragments arranged by size: 

                1938     752     310
 
BsaBI GATnn'nnATC

Cuts at:      0     278     401     821    1971    3000 
   Size:        278     123     420    1150    1029

  Fragments arranged by size: 

                1150    1029     420     278     123
 
BsaHI Gr'CG_yC

Cuts at:      0      36    3000 
   Size:         36    2964
 
BsaWI w'CCGG_w

Cuts at:      0    2576    3000 
   Size:       2576     424
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     676     706    3000 
   Size:        676      30    2294

  Fragments arranged by size: 

                2294     676      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2087    2744    3000 
   Size:       2087     657     256

  Fragments arranged by size: 

                2087     657     256
 
BseYI C'CCAG_C

Cuts at:      0     750    1792    3000 
   Size:        750    1042    1208

  Fragments arranged by size: 

                1208    1042     750
 
BsiHKAI G_wGCw'C

Cuts at:      0    1110    2787    3000 
   Size:       1110    1677     213

  Fragments arranged by size: 

                1677    1110     213
 
BsmI GAATG_Cn'

Cuts at:      0     757    1680    2814    3000 
   Size:        757     923    1134     186

  Fragments arranged by size: 

                1134     923     757     186
 
Bsp1286I G_dGCh'C

Cuts at:      0     989    1110    2787    3000 
   Size:        989     121    1677     213

  Fragments arranged by size: 

                1677     989     213     121
 
BspEI T'CCGG_A

Cuts at:      0    2576    3000 
   Size:       2576     424
 
BsrDI GCAATG_nn'

Cuts at:      0    2434    2780    3000 
   Size:       2434     346     220

  Fragments arranged by size: 

                2434     346     220
 
BsrGI T'GTAC_A

Cuts at:      0     266    1083    3000 
   Size:        266     817    1917

  Fragments arranged by size: 

                1917     817     266
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     250    3000 
   Size:        250    2750
 
BstYI r'GATC_y

Cuts at:      0     816    1966    3000 
   Size:        816    1150    1034

  Fragments arranged by size: 

                1150    1034     816
 
BtgI C'CryG_G

Cuts at:      0     354    1334    2060    3000 
   Size:        354     980     726     940

  Fragments arranged by size: 

                 980     940     726     354
 
BtsI GCAGTG_nn'

Cuts at:      0    2842    2909    3000 
   Size:       2842      67      91

  Fragments arranged by size: 

                2842      91      67
 
ClaI AT'CG_AT

Cuts at:      0     405    3000 
   Size:        405    2595
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2540    2575    3000 
   Size:       2540      35     425

  Fragments arranged by size: 

                2540     425      35
 
DraI TTT'AAA

Cuts at:      0     114     168     401     993    2280    3000 
   Size:        114      54     233     592    1287     720

  Fragments arranged by size: 

                1287     720     592     233     114      54
 
DraIII CAC_nnn'GTG

Cuts at:      0    1384    2599    3000 
   Size:       1384    1215     401

  Fragments arranged by size: 

                1384    1215     401
 
EaeI y'GGCC_r

Cuts at:      0      29    3000 
   Size:         29    2971
 
EarI CTCTTCn'nnn_

Cuts at:      0     394     918    1259    1907    2720    3000 
   Size:        394     524     341     648     813     280

  Fragments arranged by size: 

                 813     648     524     394     341     280
 
EcoICRI GAG'CTC

Cuts at:      0    2785    3000 
   Size:       2785     215
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     703    1883    1931    2087    2162    2360    2672 
   Size:         34     669    1180      48     156      75     198     312

Cuts at:   2672    2783    3000 
   Size:        111     217

  Fragments arranged by size: 

                1180     669     312     217     198     156     111      75
                  48      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2102    3000 
   Size:       2102     898
 
EcoO109I rG'GnC_Cy

Cuts at:      0     844     985     986    3000 
   Size:        844     141       1    2014

  Fragments arranged by size: 

                2014     844     141       1
 
EcoRI G'AATT_C

Cuts at:      0     542    1694    3000 
   Size:        542    1152    1306

  Fragments arranged by size: 

                1306    1152     542
 
EcoRV GAT'ATC

Cuts at:      0    1907    3000 
   Size:       1907    1093
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     639    1552    3000 
   Size:        639     913    1448

  Fragments arranged by size: 

                1448     913     639
 
HaeII r_GCGC'y

Cuts at:      0      39    3000 
   Size:         39    2961
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     566     598     676     708    1916    1948    2279    2311 
   Size:        566      32      78      32    1208      32     331      32

Cuts at:   2311    2619    2651    2697    2729    3000 
   Size:        308      32      46      32     271

  Fragments arranged by size: 

                1208     566     331     308     271      78      46      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     904    3000 
   Size:        904    2096
 
HindIII A'AGCT_T

Cuts at:      0    1120    3000 
   Size:       1120    1880
 
KasI G'GCGC_C

Cuts at:      0      35    3000 
   Size:         35    2965
 
MfeI C'AATT_G

Cuts at:      0    1862    3000 
   Size:       1862    1138
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1007    3000 
   Size:       1007    1993
 
MslI CAynn'nnrTG

Cuts at:      0    1747    3000 
   Size:       1747    1253
 
MspA1I CmG'CkG

Cuts at:      0    1297    3000 
   Size:       1297    1703
 
NarI GG'CG_CC

Cuts at:      0      36    3000 
   Size:         36    2964
 
NcoI C'CATG_G

Cuts at:      0    2060    3000 
   Size:       2060     940
 
NdeI CA'TA_TG

Cuts at:      0    1404    1818    1838    3000 
   Size:       1404     414      20    1162

  Fragments arranged by size: 

                1404    1162     414      20
 
NsiI A_TGCA'T

Cuts at:      0    1170    3000 
   Size:       1170    1830
 
NspI r_CATG'y

Cuts at:      0     773    1207    2691    3000 
   Size:        773     434    1484     309

  Fragments arranged by size: 

                1484     773     434     309
 
PciI A'CATG_T

Cuts at:      0    2687    3000 
   Size:       2687     313
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1774    3000 
   Size:       1774    1226
 
PfoI T'CCnGG_A

Cuts at:      0    1071    2649    3000 
   Size:       1071    1578     351

  Fragments arranged by size: 

                1578    1071     351
 
PpuMI rG'GwC_Cy

Cuts at:      0     844    3000 
   Size:        844    2156
 
PsiI TTA'TAA

Cuts at:      0    2884    3000 
   Size:       2884     116
 
PspOMI G'GGCC_C

Cuts at:      0     985    3000 
   Size:        985    2015
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     711     743    3000 
   Size:        711      32    2257

  Fragments arranged by size: 

                2257     711      32
 
PstI C_TGCA'G

Cuts at:      0     611    1325    1459    3000 
   Size:        611     714     134    1541

  Fragments arranged by size: 

                1541     714     611     134
 
SacI G_AGCT'C

Cuts at:      0    2787    3000 
   Size:       2787     213
 
SalI G'TCGA_C

Cuts at:      0     902    3000 
   Size:        902    2098
 
ScaI AGT'ACT

Cuts at:      0     632    3000 
   Size:        632    2368
 
SfcI C'TryA_G

Cuts at:      0     465     607     897    1229    1239    1321    1455    1493 
   Size:        465     142     290     332      10      82     134      38

Cuts at:   1493    1592    3000 
   Size:         99    1408

  Fragments arranged by size: 

                1408     465     332     290     142     134      99      82
                  38      10
 
SfoI GGC'GCC

Cuts at:      0      37    3000 
   Size:         37    2963
 
SmlI C'TyrA_G

Cuts at:      0     130    1160    1953    2263    3000 
   Size:        130    1030     793     310     737

  Fragments arranged by size: 

                1030     793     737     310     130
 
StyI C'CwwG_G

Cuts at:      0     553    1942    2060    3000 
   Size:        553    1389     118     940

  Fragments arranged by size: 

                1389     940     553     118
 
SwaI ATTT'AAAT

Cuts at:      0     401    3000 
   Size:        401    2599
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     957    1699    1944    2551    3000 
   Size:        957     742     245     607     449

  Fragments arranged by size: 

                 957     742     607     449     245
 
TatI w'GTAC_w

Cuts at:      0     266     630     727    1083    1302    2273    2977    3000 
   Size:        266     364      97     356     219     971     704      23

  Fragments arranged by size: 

                 971     704     364     356     266     219      97      23
            
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     527     951    3000 
   Size:        527     424    2049

  Fragments arranged by size: 

                2049     527     424
 
XhoI C'TCGA_G

Cuts at:      0    1160    3000 
   Size:       1160    1840
 
XmnI GAAnn'nnTTC

Cuts at:      0    2351    3000 
   Size:       2351     649
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AleI    AlwNI     ApaI    ApaLI
     ApoI     AseI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BciVI    BfrBI    BglII     BlpI Bme1580I     BpmI    BpuEI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspEI    BsrDI    BsrGI   BstAPI    BstYI     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     KasI     MfeI     MmeI     MslI   MspA1I     NarI     NcoI     NdeI
     NsiI     NspI     PciI    PflMI     PfoI    PpuMI     PsiI   PspOMI
     PsrI     PstI     SacI     SalI     ScaI     SfcI     SfoI     SmlI
     StyI     SwaI    TaqII     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI
     AloI     AscI    AsiSI     BaeI    BamHI    BbvCI     BcgI     BclI
     BglI    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     DrdI     EagI     EciI     FseI     FspI    FspAI     HpaI     KpnI
     MluI     MscI     NaeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PmeI     PmlI     PpiI    PshAI    PspXI     PvuI    PvuII
    RsrII    SacII    SanDI     SapI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     TstI  Tth111I     XbaI     XmaI     ZraI
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