Restriction map for pF1KA0519
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AflIII      
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BbeI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BsaBI       BsaI        BseAI       
BseRI       BsiEI       BsiWI       BspDI       BspEI       
BspLU11I    BssHII      Bst1107I    BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflMI       PinAI       PmeI        PshAI       
PshBI       PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SnaBI       Sse8387I    SspI        StuI        SwaI        
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          586  GTCTAC               GT^[AC][GT]AC
AccII          33  CGCG                 CG^CG
AclI          673  AACGTT               AA^CGTT
AcyI         2134  GGCGTC               G[GA]^CG[CT]C
AocI         2587  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1956  AGCGCT               AGC^GCT
AspI          937  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         630  GGATCC               G^GATCC
BbsI          121  GTCTTC               GAAGAC(2/6)
BpuAI         121  GTCTTC               GAAGAC(2/6)
BsaHI        2134  GGCGTC               G[GA]^CG[CT]C
BsaWI        1130  ACCGGA               [AT]^CCGG[AT]
BsgI         1156  GTGCAG               GTGCAG(16/14)
BsmBI        1735  GAGACG               CGTCTC(1/5)
BsmI          417  GCATTC               GAATGC(1/-1)
Bsp1407I     2678  TGTACA               T^GTACA
BspHI        2601  TCATGA               T^CATGA
BsrGI        2678  TGTACA               T^GTACA
BstAPI        375  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2550  GGTGACC              G^GT[ACGT]ACC
BstPI        2550  GGTGACC              G^GT[ACGT]ACC
BstUI          33  CGCG                 CG^CG
Bsu36I       2587  CCTCAGG              CC^T[ACGT]AGG
DraIII       1238  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1956  AGCGCT               AGC^GCT
Eco81I       2587  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      2550  GGTGACC              G^GT[ACGT]ACC
HgaI         2135  GCGTC                GACGC(5/10)
Hin1I        2134  GGCGTC               G[GA]^CG[CT]C
HincII       2001  GTTGAC               GT[CT]^[GA]AC
HindII       2001  GTTGAC               GT[CT]^[GA]AC
MseI         1580  TTAA                 T^TAA
MvnI           33  CGCG                 CG^CG
PflFI         937  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      673  AACGTT               AA^CGTT
RcaI         2601  TCATGA               T^CATGA
ScaI          874  AGTACT               AGT^ACT
SmlI          391  CTCAAG               C^T[CT][GA]AG
SpeI          720  ACTAGT               A^CTAGT
SphI           56  GCATGC               GCATG^C
SspBI        2678  TGTACA               T^GTACA
Tru9I        1580  TTAA                 T^TAA
Tth111I       937  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

BbrPI          87  CACGTG               CAC^GTG
              232  CACGTG              
BfaI          721  CTAG                 C^TAG
             1064  CTAG                
BglI          789  GCCACACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              886  GCCATGGTGGC         
BlpI           93  GCTCAGC              GC^T[ACGT]AGC
              760  GCTGAGC             
Bpu1102I       93  GCTCAGC              GC^T[ACGT]AGC
              760  GCTGAGC             
BstBI        1087  TTCGAA               TT^CGAA
             1354  TTCGAA              
CelII          93  GCTCAGC              GC^T[ACGT]AGC
              760  GCTGAGC             
DrdI          604  GACCAGTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2128  GACATTGGCGTC        
DsaI          887  CCATGG               C^C[GA][CT]GG
             2387  CCATGG              
EaeI          879  TGGCCG               [CT]^GGCC[GA]
             2316  TGGCCG              
EcoNI        1068  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2064  CCTGAACAAGG         
EcoRI        1189  GAATTC               G^AATTC
             2088  GAATTC              
HaeII         296  AGCGCC               [GA]GCGC^[CT]
             1956  AGCGCT              
MaeI          721  CTAG                 C^TAG
             1064  CTAG                
NaeI          496  GCCGGC               GCC^GGC
              530  GCCGGC              
NcoI          887  CCATGG               C^CATGG
             2387  CCATGG              
NgoMIV        496  GCCGGC               G^CCGGC
              530  GCCGGC              
NspV         1087  TTCGAA               TT^CGAA
             1354  TTCGAA              
PmaCI          87  CACGTG               CAC^GTG
              232  CACGTG              
PmlI           87  CACGTG               CAC^GTG
              232  CACGTG              
PstI          280  CTGCAG               CTGCA^G
             1426  CTGCAG              
SacI          274  GAGCTC               GAGCT^C
              430  GAGCTC              
SfcI          280  CTGCAG               C^T[GA][CT]AG
             1426  CTGCAG              
SfuI         1087  TTCGAA               TT^CGAA
             1354  TTCGAA              
SmaI          200  CCCGGG               CCC^GGG
             1306  CCCGGG              
XmaCI         200  CCCGGG               C^CCGGG
             1306  CCCGGG              
XmaI          200  CCCGGG               C^CCGGG
             1306  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaAI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PvuII       RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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