Restriction map for pF1KA0491
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HapII       
Hin1I       HincII      HindII      HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MamI        
MflI        MluI        MroI        MslI        MspA1I      
MspI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII          61  CGCG                 CG^CG
AciI           34  GCGG                 CCGC(-3/-1)
AflII         901  CTTAAG               C^TTAAG
AlwNI         311  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         738  GGACC                G^G[AT]CC
BalI           90  TGGCCA               TGG^CCA
BbsI          102  GAAGAC               GAAGAC(2/6)
BclI          992  TGATCA               T^GATCA
BfrI          901  CTTAAG               C^TTAAG
BpmI          742  CTCCAG               CTGGAG(16/14)
BpuAI         102  GAAGAC               GAAGAC(2/6)
BsgI           67  GTGCAG               GTGCAG(16/14)
BsiEI          35  CGGCCG               CG[GA][CT]^CG
BsiYI         155  CCAAAATATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          155  CCAAAATATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BstAPI        544  GCAAAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          61  CGCG                 CG^CG
CfoI           62  GCGC                 GCG^C
DsaI          848  CCATGG               C^C[GA][CT]GG
EagI           35  CGGCCG               C^GGCCG
EclXI          35  CGGCCG               C^GGCCG
Eco52I         35  CGGCCG               C^GGCCG
EcoT14I       848  CCATGG               C^C[AT][AT]GG
FbaI          992  TGATCA               T^GATCA
HgaI           40  GACGC                GACGC(5/10)
HhaI           62  GCGC                 GCG^C
HinP1I         62  GCGC                 G^CGC
HindIII        85  AAGCTT               A^AGCTT
MfeI          470  CAATTG               C^AATTG
MluNI          90  TGGCCA               TGG^CCA
MscI           90  TGGCCA               TGG^CCA
MunI          470  CAATTG               C^AATTG
MvnI           61  CGCG                 CG^CG
NcoI          848  CCATGG               C^CATGG
NspI          657  ACATGC               [GA]CATG^[CT]
PflMI         155  CCAAAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          551  CTGCAG               CTGCA^G
ScaI          961  AGTACT               AGT^ACT
SfcI          551  CTGCAG               C^T[GA][CT]AG
SmlI          901  CTTAAG               C^T[CT][GA]AG
StyI          848  CCATGG               C^C[AT][AT]GG
Van91I        155  CCAAAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          372  CCAAAAAAGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

Asp700I       400  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1016  GAATGGATTC          
BanI           46  GGCACC               G^G[CT][GA]CC
             1065  GGTGCC              
BsmAI         504  GAGAC                GTCTC(1/5)
              531  GAGAC               
BsmFI         291  GGGAC                GGGAC(10/14)
              313  GGGAC               
Cac8I          30  GCTGGC               GC[ACGT]^[ACGT]GC
               43  GCAGGC              
Cfr13I        325  GGCCC                G^G[ACGT]CC
              738  GGACC               
DpnII         607  GATC                 ^GATC
              993  GATC                
EaeI           35  CGGCCG               [CT]^GGCC[GA]
               90  TGGCCA              
MaeII          20  ACGT                 A^CGT
              412  ACGT                
MboI          607  GATC                 ^GATC
              993  GATC                
MwoI           34  GCGGCCGACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              544  GCAAAAGCTGC         
NdeII         607  GATC                 ^GATC
              993  GATC                
NlaIV          46  GGCACC               GG[ACGT]^[ACGT]CC
             1065  GGTGCC              
Sau3AI        607  GATC                 ^GATC
              993  GATC                
Sau96I        325  GGCCC                G^G[ACGT]CC
              738  GGACC               
Tsp45I        802  GTGAC                ^GT[GC]AC
              896  GTGAC               
XmnI          400  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1016  GAATGGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BbvI        
BfaI        BsaJI       BsrI        BssKI       BstF5I      
BstNI       DdeI        EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboII       MnlI        MseI        MvaI        
NlaIII      RsaI        ScrFI       SfaNI       TfiI        
Tru9I       TseI        Tsp509I     TspRI       
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