Restriction map for pF1KA0481
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvrII       BamHI       BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoRI       
EcoRV       FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        MaeI        MamI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacI        SalI        ScaI        SfiI        
SfuI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Tsp509I     
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII         300  GACGTC               GACGT^C
AccIII        378  TCCGGA               T^CCGGA
AocI         1360  CCTGAGG              CC^T[ACGT]AGG
AspI          742  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2110  CACGTG               CAC^GTG
BbvCI         718  GCTGAGG              CCTCAGC(-5/-2)
BcgI          918  GCAGGAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1454  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1454  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2110  CACGTG               [CT]AC^GT[GA]
BseAI         378  TCCGGA               T^CCGGA
BsmI         1905  GAATGC               GAATGC(1/-1)
BspEI         378  TCCGGA               T^CCGGA
BspHI        2012  TCATGA               T^CATGA
BssHII       1173  GCGCGC               G^CGCGC
BstEII       1845  GGTCACC              G^GT[ACGT]ACC
BstPI        1845  GGTCACC              G^GT[ACGT]ACC
Bsu36I       1360  CCTGAGG              CC^T[ACGT]AGG
CelII        1454  GCTCAGC              GC^T[ACGT]AGC
DraI          201  TTTAAA               TTT^AAA
Eco81I       1360  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1845  GGTCACC              G^GT[ACGT]ACC
EcoT22I       385  ATGCAT               ATGCA^T
KspI          161  CCGCGG               CCGC^GG
MroI          378  TCCGGA               T^CCGGA
MseI          202  TTAA                 T^TAA
NruI          927  TCGCGA               TCG^CGA
NsiI          385  ATGCAT               ATGCA^T
NspI         1136  ACATGC               [GA]CATG^[CT]
PflFI         742  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2110  CACGTG               CAC^GTG
PmlI         2110  CACGTG               CAC^GTG
PshAI        1839  GACCCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2012  TCATGA               T^CATGA
SacII         161  CCGCGG               CCGC^GG
SbfI         1662  CCTGCAGG             CCTGCA^GG
SexAI         761  ACCTGGT              A^CC[AT]GGT
Sse8387I     1662  CCTGCAGG             CCTGCA^GG
TfiI          310  GATTC                G^A[AT]TC
Tru9I         202  TTAA                 T^TAA
Tth111I       742  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AflIII        704  ACGTGT               A^C[GA][CT]GT
             2111  ACGTGT              
Aor51HI       371  AGCGCT               AGC^GCT
             1673  AGCGCT              
ApaI         1102  GGGCCC               GGGCC^C
             1354  GGGCCC              
AviII         593  TGCGCA               TGC^GCA
             2027  TGCGCA              
BsaWI         378  TCCGGA               [AT]^CCGG[AT]
              837  ACCGGA              
BstAPI        414  GCACGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2109  GCACGTGTTGC         
BstXI         809  CCATCAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2042  CCACCCTCCTGG        
BstYI         461  AGATCC               [GA]^GATC[CT]
              884  AGATCC              
Eco47III      371  AGCGCT               AGC^GCT
             1673  AGCGCT              
FspI          593  TGCGCA               TGC^GCA
             2027  TGCGCA              
MflI          461  AGATCC               [GA]^GATC[CT]
              884  AGATCC              
NcoI          789  CCATGG               C^CATGG
             1607  CCATGG              
PpuMI         177  AGGTCCC              [GA]G^G[AT]CC[CT]
             1337  AGGACCC             
SapI         1561  GAAGAGC              GCTCTTC(1/4)
              656  GCTCTTC             
SgrAI         150  CGCCGGCG             C[GA]^CCGG[CT]G
             1072  CGCCGGCG            
TaqI          749  TCGA                 T^CGA
             1007  TCGA                
TthHB8I       749  TCGA                 T^CGA
             1007  TCGA                
XhoII         461  AGATCC               [GA]^GATC[CT]
              884  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        StyI        TseI        
Tsp45I      TspRI       XmaCI       XmaI        
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