Restriction map for pF1KA0481
 (Linear) (Six-base) MAPSORT of: KIAA0481.seq  Check: 5744  from: 1  to: 2127



KIAA0481.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      89     596     881     972    1869    2127 
   Size:         89     507     285      91     897     258

  Fragments arranged by size: 

                 897     507     285     258      91      89
 
AatII G_ACGT'C

Cuts at:      0     304    2127 
   Size:        304    1823
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     271     910    1102    1171    1354    1639    1768 
   Size:        128     143     639     192      69     183     285     129

Cuts at:   1768    2127 
   Size:        359

  Fragments arranged by size: 

                 639     359     285     192     183     143     129     128
                  69
 
AfeI AGC'GCT

Cuts at:      0     373    1675    2127 
   Size:        373    1302     452

  Fragments arranged by size: 

                1302     452     373
 
AflIII A'CryG_T

Cuts at:      0     704    2111    2127 
   Size:        704    1407      16

  Fragments arranged by size: 

                1407     704      16
 
AleI CACnn'nnGTG

Cuts at:      0    1258    2127 
   Size:       1258     869
 
AlwNI CAG_nnn'CTG

Cuts at:      0     219    1149    1662    1758    2127 
   Size:        219     930     513      96     369

  Fragments arranged by size: 

                 930     513     369     219      96
 
ApaI G_GGCC'C

Cuts at:      0    1106    1358    2127 
   Size:       1106     252     769

  Fragments arranged by size: 

                1106     769     252
 
AvaI C'yCGr_G

Cuts at:      0     281    1274    1373    1841    2127 
   Size:        281     993      99     468     286

  Fragments arranged by size: 

                 993     468     286     281      99
 
BanI G'GyrC_C

Cuts at:      0     165     490     834    1076    1162    2127 
   Size:        165     325     344     242      86     965

  Fragments arranged by size: 

                 965     344     325     242     165      86
 
BanII G_rGCy'C

Cuts at:      0     232     569    1106    1358    1510    2127 
   Size:        232     337     537     252     152     617

  Fragments arranged by size: 

                 617     537     337     252     232     152
 
BbeI G_GCGC'C

Cuts at:      0     169     494    1080    1166    2127 
   Size:        169     325     586      86     961

  Fragments arranged by size: 

                 961     586     325     169      86
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1619    1627    2023    2127 
   Size:       1619       8     396     104

  Fragments arranged by size: 

                1619     396     104       8
 
BbvCI CC'TCA_GC

Cuts at:      0     719    2127 
   Size:        719    1408
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     907     941    2127 
   Size:        907      34    1186

  Fragments arranged by size: 

                1186     907      34
 
BfrBI ATG'CAT

Cuts at:      0     387    2127 
   Size:        387    1740
 
BglI GCCn_nnn'nGGC

Cuts at:      0     429    1110    1232    2127 
   Size:        429     681     122     895

  Fragments arranged by size: 

                 895     681     429     122
 
BlpI GC'TnA_GC

Cuts at:      0    1455    2127 
   Size:       1455     672
 
Bme1580I G_kGCm'C

Cuts at:      0     827     837    1106    1358    1380    1806    2127 
   Size:        827      10     269     252      22     426     321

  Fragments arranged by size: 

                 827     426     321     269     252      22      10
 
BmgBI CAC'GTC

Cuts at:      0     822    1125    1158    2127 
   Size:        822     303      33     969

  Fragments arranged by size: 

                 969     822     303      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1650    2091    2127 
   Size:       1650     441      36

  Fragments arranged by size: 

                1650     441      36
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583    1081    1389    1422    1618    1714    1876    1912 
   Size:        583     498     308      33     196      96     162      36

Cuts at:   1912    2125    2127 
   Size:        213       2

  Fragments arranged by size: 

                 583     498     308     213     196     162      96      36
                  33       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     719     772    2127 
   Size:        719      53    1355

  Fragments arranged by size: 

                1355     719      53
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     103     284     752    2127 
   Size:        103     181     468    1375

  Fragments arranged by size: 

                1375     468     181     103
 
BsaAI yAC'GTr

Cuts at:      0    2112    2127 
   Size:       2112      15
 
BsaHI Gr'CG_yC

Cuts at:      0     149     166     301     491    1077    1163    1214    2127 
   Size:        149      17     135     190     586      86      51     913

  Fragments arranged by size: 

                 913     586     190     149     135      86      51      17
            
 
BsaWI w'CCGG_w

Cuts at:      0     378     837    2127 
   Size:        378     459    1290

  Fragments arranged by size: 

                1290     459     378
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     337     367     749     779    1573    1603    2071    2098 
   Size:        337      30     382      30     794      30     468      27

Cuts at:   2098    2101    2127 
   Size:          3      26

  Fragments arranged by size: 

                 794     468     382     337      30      30      30      27
                  26       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     736    1378    1684    1711    1786    2059    2127 
   Size:         58     678     642     306      27      75     273      68

  Fragments arranged by size: 

                 678     642     306     273      75      68      58      27
            
 
BseYI C'CCAG_C

Cuts at:      0      55     314     569     845    1043    1483    1500    1926 
   Size:         55     259     255     276     198     440      17     426

Cuts at:   1926    2122    2127 
   Size:        196       5

  Fragments arranged by size: 

                 440     426     276     259     255     198     196      55
                  17       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     397     484     616     859    1033    2127 
   Size:         30     367      87     132     243     174    1094

  Fragments arranged by size: 

                1094     367     243     174     132      87      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     296    1067    1438    1536    2111    2127 
   Size:        296     771     371      98     575      16

  Fragments arranged by size: 

                 771     575     371     296      98      16
 
BsmI GAATG_Cn'

Cuts at:      0    1911    2127 
   Size:       1911     216
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     308     399    1407    2127 
   Size:        308      91    1008     720

  Fragments arranged by size: 

                1008     720     308      91
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     296     569     827     837    1067    1106    1358 
   Size:        232      64     273     258      10     230      39     252

Cuts at:   1358    1380    1438    1510    1536    1806    2111    2127 
   Size:         22      58      72      26     270     305      16

  Fragments arranged by size: 

                 305     273     270     258     252     232     230      72
                  64      58      39      26      22      16      10
 
BspEI T'CCGG_A

Cuts at:      0     378    2127 
   Size:        378    1749
 
BspHI T'CATG_A

Cuts at:      0    2012    2127 
   Size:       2012     115
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      89     428     596     881     972    1869    2127 
   Size:         89     339     168     285      91     897     258

  Fragments arranged by size: 

                 897     339     285     258     168      91      89
 
BsrBI CCG'CTC

Cuts at:      0     467     656    1385    1689    2127 
   Size:        467     189     729     304     438

  Fragments arranged by size: 

                 729     467     438     304     189
 
BsrFI r'CCGG_y

Cuts at:      0     151     539     784    1073    2127 
   Size:        151     388     245     289    1054

  Fragments arranged by size: 

                1054     388     289     245     151
 
BssHII G'CGCG_C

Cuts at:      0    1173    2127 
   Size:       1173     954
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     420    2115    2127 
   Size:        420    1695      12

  Fragments arranged by size: 

                1695     420      12
 
BstEII G'GTnAC_C

Cuts at:      0    1845    2127 
   Size:       1845     282
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     816    2049    2127 
   Size:        816    1233      78

  Fragments arranged by size: 

                1233     816      78
 
BstYI r'GATC_y

Cuts at:      0     461     884    2127 
   Size:        461     423    1243

  Fragments arranged by size: 

                1243     461     423
 
Bsu36I CC'TnA_GG

Cuts at:      0    1361    2127 
   Size:       1361     766
 
BtgI C'CryG_G

Cuts at:      0     113     161     243     789    1259    1607    1823    2127 
   Size:        113      48      82     546     470     348     216     304

  Fragments arranged by size: 

                 546     470     348     304     216     113      82      48
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     373    1023    1308    1439    1974    2127 
   Size:        373     650     285     131     535     153

  Fragments arranged by size: 

                 650     535     373     285     153     131
 
BtsI GCAGTG_nn'

Cuts at:      0     516     674    1317    1395    2127 
   Size:        516     158     643      78     732

  Fragments arranged by size: 

                 732     643     516     158      78
 
DraI TTT'AAA

Cuts at:      0     203    2127 
   Size:        203    1924
 
EaeI y'GGCC_r

Cuts at:      0     240     620     689     786     959    1760    1961    2127 
   Size:        240     380      69      97     173     801     201     166

  Fragments arranged by size: 

                 801     380     240     201     173     166      97      69
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     663    1556    2127 
   Size:        663     893     571

  Fragments arranged by size: 

                 893     663     571
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     271     583     910    1081    1102    1171    1354 
   Size:        128     143     312     327     171      21      69     183

Cuts at:   1354    1389    1422    1618    1639    1714    1768    1876    1912 
   Size:         35      33     196      21      75      54     108      36

Cuts at:   1912    2125    2127 
   Size:        213       2

  Fragments arranged by size: 

                 327     312     213     196     183     171     143     128
                 108      75      69      54      36      35      33      21
                  21       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     718    1240    1666    1750    1797    2127 
   Size:        718     522     426      84      47     330

  Fragments arranged by size: 

                 718     522     426     330      84      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0     178     600    1103    1338    1355    2127 
   Size:        178     422     503     235      17     772

  Fragments arranged by size: 

                 772     503     422     235     178      17
 
FspI TGC'GCA

Cuts at:      0     595    2029    2127 
   Size:        595    1434      98

  Fragments arranged by size: 

                1434     595      98
 
HaeII r_GCGC'y

Cuts at:      0     158     169     375     494    1080    1166    1445    1451 
   Size:        158      11     206     119     586      86     279       6

Cuts at:   1451    1501    1677    1924    1957    2127 
   Size:         50     176     247      33     170

  Fragments arranged by size: 

                 586     279     247     206     176     170     158     119
                  86      50      33      11       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     337     369    1420    1452    1573    1605    2127 
   Size:        337      32    1051      32     121      32     522

  Fragments arranged by size: 

                1051     522     337     121      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     165     490    1076    1162    2127 
   Size:        165     325     586      86     965

  Fragments arranged by size: 

                 965     586     325     165      86
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368    2127 
   Size:        368    1759
 
MscI TGG'CCA

Cuts at:      0     242     961    1762    2127 
   Size:        242     719     801     365

  Fragments arranged by size: 

                 801     719     365     242
 
MslI CAynn'nnrTG

Cuts at:      0    1258    1648    1942    1963    2127 
   Size:       1258     390     294      21     164

  Fragments arranged by size: 

                1258     390     294     164      21
 
MspA1I CmG'CkG

Cuts at:      0     163     219     527     849    1047    1187    1629    1890 
   Size:        163      56     308     322     198     140     442     261

Cuts at:   1890    2127 
   Size:        237

  Fragments arranged by size: 

                 442     322     308     261     237     198     163     140
                  56
 
NaeI GCC'GGC

Cuts at:      0     153     541     786    1075    2127 
   Size:        153     388     245     289    1052

  Fragments arranged by size: 

                1052     388     289     245     153
 
NarI GG'CG_CC

Cuts at:      0     166     491    1077    1163    2127 
   Size:        166     325     586      86     964

  Fragments arranged by size: 

                 964     586     325     166      86
 
NcoI C'CATG_G

Cuts at:      0     789    1607    2127 
   Size:        789     818     520

  Fragments arranged by size: 

                 818     789     520
 
NgoMIV G'CCGG_C

Cuts at:      0     151     539     784    1073    2127 
   Size:        151     388     245     289    1054

  Fragments arranged by size: 

                1054     388     289     245     151
 
NruI TCG'CGA

Cuts at:      0     929    2127 
   Size:        929    1198
 
NsiI A_TGCA'T

Cuts at:      0     389    2127 
   Size:        389    1738
 
NspI r_CATG'y

Cuts at:      0    1140    2127 
   Size:       1140     987
 
PfoI T'CCnGG_A

Cuts at:      0    1536    1814    2102    2127 
   Size:       1536     278     288      25

  Fragments arranged by size: 

                1536     288     278      25
 
PmlI CAC'GTG

Cuts at:      0    2112    2127 
   Size:       2112      15
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1090    1122    2127 
   Size:       1090      32    1005

  Fragments arranged by size: 

                1090    1005      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     178    1338    2127 
   Size:        178    1160     789

  Fragments arranged by size: 

                1160     789     178
 
PshAI GACnn'nnGTC

Cuts at:      0    1843    2127 
   Size:       1843     284
 
PspOMI G'GGCC_C

Cuts at:      0    1102    1354    2127 
   Size:       1102     252     773

  Fragments arranged by size: 

                1102     773     252
 
PstI C_TGCA'G

Cuts at:      0      29     458    1634    1667    1865    2127 
   Size:         29     429    1176      33     198     262

  Fragments arranged by size: 

                1176     429     262     198      33      29
 
PvuII CAG'CTG

Cuts at:      0     219    1047    1629    1890    2127 
   Size:        219     828     582     261     237

  Fragments arranged by size: 

                 828     582     261     237     219
 
SacII CC_GC'GG

Cuts at:      0     164    2127 
   Size:        164    1963
 
SapI GCTCTTCn'nnn_

Cuts at:      0     663    1556    2127 
   Size:        663     893     571

  Fragments arranged by size: 

                 893     663     571
 
SbfI CC_TGCA'GG

Cuts at:      0    1667    2127 
   Size:       1667     460
 
SexAI A'CCwGG_T

Cuts at:      0     761    2127 
   Size:        761    1366
 
SfcI C'TryA_G

Cuts at:      0      25     454    1630    1663    1677    1861    2127 
   Size:         25     429    1176      33      14     184     266

  Fragments arranged by size: 

                1176     429     266     184      33      25      14
 
SfoI GGC'GCC

Cuts at:      0     167     492    1078    1164    2127 
   Size:        167     325     586      86     963

  Fragments arranged by size: 

                 963     586     325     167      86
 
SgrAI Cr'CCGG_yG

Cuts at:      0     151    1073    2127 
   Size:        151     922    1054

  Fragments arranged by size: 

                1054     922     151
 
SmaI CCC'GGG

Cuts at:      0    1276    1375    1843    2127 
   Size:       1276      99     468     284

  Fragments arranged by size: 

                1276     468     284      99
 
SrfI GCCC'GGGC

Cuts at:      0    1375    2127 
   Size:       1375     752
 
StyI C'CwwG_G

Cuts at:      0     789     854    1118    1607    2127 
   Size:        789      65     264     489     520

  Fragments arranged by size: 

                 789     520     489     264      65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     266    1264    2127 
   Size:        266     998     863

  Fragments arranged by size: 

                 998     863     266
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     749     781    1796    1828    2071    2103    2127 
   Size:        749      32    1015      32     243      32      24

  Fragments arranged by size: 

                1015     749     243      32      32      32      24
 
Tth111I GACn'n_nGTC

Cuts at:      0     745    2127 
   Size:        745    1382
 
XmaI C'CCGG_G

Cuts at:      0    1274    1373    1841    2127 
   Size:       1274      99     468     286

  Fragments arranged by size: 

                1274     468     286      99
 
ZraI GAC'GTC

Cuts at:      0     302    2127 
   Size:        302    1825
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FspI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NruI     NsiI     NspI     PfoI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII    SacII     SapI
     SbfI    SexAI     SfcI     SfoI    SgrAI     SmaI     SrfI     StyI
    TaqII     TstI  Tth111I     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BciVI     BclI    BglII     BmtI     BplI    BpuEI    BsaBI    BsiEI
    BsiWI    BsrDI    BsrGI    BssSI    BstBI  BstZ17I     ClaI    CspCI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     PacI     PasI     PciI    PflMI     PmeI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI     SalI    SanDI
     ScaI     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     SwaI     TatI     XbaI     XcmI     XhoI     XmnI
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