Restriction map for pF1KA0471
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BlnI        
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        ClaI        CpoI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       StuI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcsI          953  AAATTC               [GA]^AATT[CT]
AflII         880  CTTAAG               C^TTAAG
AgeI         1080  ACCGGT               A^CCGGT
AlwI          495  GGATC                GGATC(4/5)
AlwNI         550  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          953  AAATTC               [GA]^AATT[CT]
Asp700I       178  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         843  GGACC                G^G[AT]CC
BfrI          880  CTTAAG               C^TTAAG
BglII         689  AGATCT               A^GATCT
BlpI           34  GCTAAGC              GC^T[ACGT]AGC
BpmI          715  CTGGAG               CTGGAG(16/14)
Bpu1102I       34  GCTAAGC              GC^T[ACGT]AGC
BsaWI        1080  ACCGGT               [AT]^CCGG[AT]
BsmFI          60  GTCCC                GGGAC(10/14)
BsrFI        1080  ACCGGT               [GA]^CCGG[CT]
BstAPI        779  GCAAACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1083  GGTCACC              G^GT[ACGT]ACC
BstF5I        131  GGATG                GGATG(2/0)
BstPI        1083  GGTCACC              G^GT[ACGT]ACC
BstYI         689  AGATCT               [GA]^GATC[CT]
CelII          34  GCTAAGC              GC^T[ACGT]AGC
Cfr10I       1080  ACCGGT               [GA]^CCGG[CT]
Cfr13I        843  GGACC                G^G[ACGT]CC
DraI          210  TTTAAA               TTT^AAA
DsaI         1052  CCACGG               C^C[GA][CT]GG
EaeI         1049  CGGCCA               [CT]^GGCC[GA]
EcoNI         183  CCTTCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1083  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1097  CCAAGG               C^C[AT][AT]GG
FokI          131  GGATG                GGATG(9/13)
HapII        1081  CCGG                 C^CGG
HpaII        1081  CCGG                 C^CGG
MflI          689  AGATCT               [GA]^GATC[CT]
MslI          784  CACTGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1081  CCGG                 C^CGG
PinAI        1080  ACCGGT               A^CCGGT
SacI          532  GAGCTC               GAGCT^C
Sau96I        843  GGACC                G^G[ACGT]CC
SfaNI         138  GCATC                GCATC(5/9)
SmlI          880  CTTAAG               C^T[CT][GA]AG
SpeI          459  ACTAGT               A^CTAGT
SphI          775  GCATGC               GCATG^C
SspI          289  AATATT               AAT^ATT
StyI         1097  CCAAGG               C^C[AT][AT]GG
TaqI          111  TCGA                 T^CGA
TthHB8I       111  TCGA                 T^CGA
XhoII         689  AGATCT               [GA]^GATC[CT]
XmnI          178  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          298  GTAC                 GT^AC
              707  GTAC                
AspHI         532  GAGCTC               G[AT]GC[AT]^C
             1104  GAGCAC              
BanI           12  GGTGCC               G^G[CT][GA]CC
             1057  GGCACC              
BanII         532  GAGCTC               G[GA]GC[CT]^C
              980  GAGCCC              
BsiHKAI       532  GAGCTC               G[AT]GC[AT]^C
             1104  GAGCAC              
BsmAI         562  GAGAC                GTCTC(1/5)
              595  GAGAC               
BsrDI         672  CATTGC               GCAATG(2/0)
             1067  CATTGC              
BsrI          906  ACTGG                ACTGG(1/-1)
              912  ACTGG               
DpnII         496  GATC                 ^GATC
              690  GATC                
HinfI         351  GAGTC                G^A[ACGT]TC
              874  GACTC               
HphI           93  GGTGA                GGTGA(8/7)
             1085  TCACC               
MboI          496  GATC                 ^GATC
              690  GATC                
NdeII         496  GATC                 ^GATC
              690  GATC                
NspI           68  ACATGC               [GA]CATG^[CT]
              775  GCATGC              
PleI          874  GACTC                GAGTC(4/5)
              351  GAGTC               
RsaI          298  GTAC                 GT^AC
              707  GTAC                
Sau3AI        496  GATC                 ^GATC
              690  GATC                
Tsp45I        791  GTGAC                ^GT[GC]AC
             1084  GTCAC               
TspRI         784  CACTG                CA[GC]TG[ACGT][ACGT]^
              789  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AluI        BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BssKI       BstNI       Cac8I       DdeI        
EarI        EcoRII      Fnu4HI      HaeIII      ItaI        
Ksp632I     MaeI        MaeIII      MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NlaIII      
NlaIV       PstI        PvuII       ScrFI       SfcI        
Tru9I       TseI        Tsp509I     XcmI        
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