Restriction map for pF1KA0471
 (Linear) (Six-base) MAPSORT of: KIAA0471.seq  Check: 3885  from: 1  to: 1110



KIAA0471.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     587     603    1110 
   Size:        334     253      16     507

  Fragments arranged by size: 

                 507     334     253      16
 
AflII C'TTAA_G

Cuts at:      0     880    1110 
   Size:        880     230
 
AgeI A'CCGG_T

Cuts at:      0    1080    1110 
   Size:       1080      30
 
AleI CACnn'nnGTG

Cuts at:      0     788    1110 
   Size:        788     322
 
AlwNI CAG_nnn'CTG

Cuts at:      0     555    1110 
   Size:        555     555
 
ApoI r'AATT_y

Cuts at:      0     953    1110 
   Size:        953     157
 
BanI G'GyrC_C

Cuts at:      0      12    1057    1110 
   Size:         12    1045      53

  Fragments arranged by size: 

                1045      53      12
 
BanII G_rGCy'C

Cuts at:      0     536     984    1110 
   Size:        536     448     126

  Fragments arranged by size: 

                 536     448     126
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     185     273     374     512     609     664    1110 
   Size:        185      88     101     138      97      55     446

  Fragments arranged by size: 

                 446     185     138     101      97      88      55
 
BglII A'GATC_T

Cuts at:      0     689    1110 
   Size:        689     421
 
BlpI GC'TnA_GC

Cuts at:      0      35    1110 
   Size:         35    1075
 
Bme1580I G_kGCm'C

Cuts at:      0      17    1060    1110 
   Size:         17    1043      50

  Fragments arranged by size: 

                1043      50      17
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     891    1110 
   Size:        891     219
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736    1110 
   Size:        736     374
 
BsaWI w'CCGG_w

Cuts at:      0    1080    1110 
   Size:       1080      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     931     961    1110 
   Size:        931      30     149

  Fragments arranged by size: 

                 931     149      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      96     586     757     992    1110 
   Size:         96     490     171     235     118

  Fragments arranged by size: 

                 490     235     171     118      96
 
BseYI C'CCAG_C

Cuts at:      0      21      27     602    1061    1088    1110 
   Size:         21       6     575     459      27      22

  Fragments arranged by size: 

                 575     459      27      22      21       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     536    1108    1110 
   Size:        536     572       2

  Fragments arranged by size: 

                 572     536       2
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     536     984    1060    1108    1110 
   Size:         17     519     448      76      48       2

  Fragments arranged by size: 

                 519     448      76      48      17       2
 
BsrDI GCAATG_nn'

Cuts at:      0     671    1066    1110 
   Size:        671     395      44

  Fragments arranged by size: 

                 671     395      44
 
BsrFI r'CCGG_y

Cuts at:      0    1080    1110 
   Size:       1080      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     785    1110 
   Size:        785     325
 
BstEII G'GTnAC_C

Cuts at:      0    1083    1110 
   Size:       1083      27
 
BstYI r'GATC_y

Cuts at:      0     689    1110 
   Size:        689     421
 
BtgI C'CryG_G

Cuts at:      0    1052    1110 
   Size:       1052      58
 
BtsI GCAGTG_nn'

Cuts at:      0     783     795    1110 
   Size:        783      12     315

  Fragments arranged by size: 

                 783     315      12
 
DraI TTT'AAA

Cuts at:      0     212    1110 
   Size:        212     898
 
EaeI y'GGCC_r

Cuts at:      0    1049    1110 
   Size:       1049      61
 
EarI CTCTTCn'nnn_

Cuts at:      0     563     571     587    1110 
   Size:        563       8      16     523

  Fragments arranged by size: 

                 563     523      16       8
 
EcoICRI GAG'CTC

Cuts at:      0     534    1110 
   Size:        534     576
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     587     603     736    1110 
   Size:        334     253      16     133     374

  Fragments arranged by size: 

                 374     334     253     133      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     187    1110 
   Size:        187     923
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     553    1110 
   Size:        553     557
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     123     155    1110 
   Size:        123      32     955

  Fragments arranged by size: 

                 955     123      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     407    1110 
   Size:        407     703
 
MslI CAynn'nnrTG

Cuts at:      0     788    1110 
   Size:        788     322
 
MspA1I CmG'CkG

Cuts at:      0      31     357     891    1110 
   Size:         31     326     534     219

  Fragments arranged by size: 

                 534     326     219      31
 
NspI r_CATG'y

Cuts at:      0      72     779    1110 
   Size:         72     707     331

  Fragments arranged by size: 

                 707     331      72
 
PstI C_TGCA'G

Cuts at:      0     191     790     932    1110 
   Size:        191     599     142     178

  Fragments arranged by size: 

                 599     191     178     142
 
PvuII CAG'CTG

Cuts at:      0      31     357     891    1110 
   Size:         31     326     534     219

  Fragments arranged by size: 

                 534     326     219      31
 
SacI G_AGCT'C

Cuts at:      0     536    1110 
   Size:        536     574
 
SfcI C'TryA_G

Cuts at:      0     187     662     786     928    1110 
   Size:        187     475     124     142     182

  Fragments arranged by size: 

                 475     187     182     142     124
 
SmlI C'TyrA_G

Cuts at:      0     880    1110 
   Size:        880     230
 
SpeI A'CTAG_T

Cuts at:      0     459    1110 
   Size:        459     651
 
SphI G_CATG'C

Cuts at:      0     779    1110 
   Size:        779     331
 
SspI AAT'ATT

Cuts at:      0     291    1110 
   Size:        291     819
 
StyI C'CwwG_G

Cuts at:      0    1097    1110 
   Size:       1097      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1075    1102    1110 
   Size:       1075      27       8

  Fragments arranged by size: 

                1075      27       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     931     963    1110 
   Size:        931      32     147

  Fragments arranged by size: 

                 931     147      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     423     552     930    1110 
   Size:        423     129     378     180

  Fragments arranged by size: 

                 423     378     180     129
 
XmnI GAAnn'nnTTC

Cuts at:      0     182    1110 
   Size:        182     928
 

 Enzymes that do cut:

     AcuI    AflII     AgeI     AleI    AlwNI     ApoI     BanI    BanII
     BbsI    BglII     BlpI Bme1580I     BplI     BpmI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI    BsrFI   BstAPI   BstEII
    BstYI     BtgI     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI     FalI    Hin4I     MmeI     MslI   MspA1I     NspI     PstI
    PvuII     SacI     SfcI     SmlI     SpeI     SphI     SspI     StyI
    TaqII     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI    BstBI
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI
     StuI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI
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