Restriction map for pF1KA0418
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BbrPI       BciVI       
BclI        BfrI        BglII       BlnI        BsiEI       
BsiWI       Bsp1407I    BspHI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     DraI        DraIII      
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       EcoT22I     FbaI        FspI        HindIII     
HpaI        KpnI        MfeI        MluI        MseI        
MunI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        SacI        SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII        308  TCCGGA               T^CCGGA
AcsI          793  GAATTC               [GA]^AATT[CT]
ApoI          793  GAATTC               [GA]^AATT[CT]
Asp700I      2772  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         123  GAGCAC               G[AT]GC[AT]^C
BalI          647  TGGCCA               TGG^CCA
BbsI         1242  GTCTTC               GAAGAC(2/6)
BcgI         2520  CGAACGGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1865  CTAG                 C^TAG
BpuAI        1242  GTCTTC               GAAGAC(2/6)
BsaBI        1737  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         308  TCCGGA               [AT]^CCGG[AT]
BseAI         308  TCCGGA               T^CCGGA
BsiHKAI       123  GAGCAC               G[AT]GC[AT]^C
BsmBI        2652  CGTCTC               CGTCTC(1/5)
BspDI         925  ATCGAT               AT^CGAT
BspEI         308  TCCGGA               T^CCGGA
BstYI        2756  AGATCC               [GA]^GATC[CT]
ClaI          925  ATCGAT               AT^CGAT
CpoI         1685  CGGTCCG              CG^G[AT]CCG
DrdI          607  GACAGGACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         793  GAATTC               G^AATTC
FseI          423  GGCCGGCC             GGCCGG^CC
HincII       2236  GTCAAC               GT[CT]^[GA]AC
HindII       2236  GTCAAC               GT[CT]^[GA]AC
MaeI         1865  CTAG                 C^TAG
MamI         1737  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         2756  AGATCC               [GA]^GATC[CT]
MluNI         647  TGGCCA               TGG^CCA
MroI          308  TCCGGA               T^CCGGA
MscI          647  TGGCCA               TGG^CCA
MslI         2577  CATCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         2711  CCATGG               C^CATGG
NdeI         1301  CATATG               CA^TATG
NspI         1960  ACATGC               [GA]CATG^[CT]
PpuMI         146  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI         1213  CTGCAG               CTGCA^G
RsrII        1685  CGGTCCG              CG^G[AT]CCG
ScaI          776  AGTACT               AGT^ACT
SfcI         1213  CTGCAG               C^T[GA][CT]AG
Tsp509I       794  AATT                 ^AATT
XhoII        2756  AGATCC               [GA]^GATC[CT]
XmnI         2772  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AhdI         1312  GACACCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1659  GACCTCCTGTC         
AocI         1160  CCTCAGG              CC^T[ACGT]AGG
             2300  CCTCAGG             
AspEI        1312  GACACCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1659  GACCTCCTGTC         
BbeI          363  GGCGCC               GGCGC^C
             1757  GGCGCC              
BbvCI         661  CCTCAGC              CCTCAGC(-5/-2)
             1478  CCTCAGC             
BlpI           57  GCTGAGC              GC^T[ACGT]AGC
             1131  GCTGAGC             
Bpu1102I       57  GCTGAGC              GC^T[ACGT]AGC
             1131  GCTGAGC             
BsaAI          16  TACGTG               [CT]AC^GT[GA]
              880  TACGTG              
BsaI         1270  GAGACC               GGTCTC(1/5)
             1767  GAGACC              
BsmI         1446  GAATGC               GAATGC(1/-1)
             1105  GCATTC              
BstXI         155  CCACCTACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1779  CCAGCTCCGTGG        
Bsu36I       1160  CCTCAGG              CC^T[ACGT]AGG
             2300  CCTCAGG             
CelII          57  GCTGAGC              GC^T[ACGT]AGC
             1131  GCTGAGC             
Eam1105I     1312  GACACCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1659  GACCTCCTGTC         
Eco81I       1160  CCTCAGG              CC^T[ACGT]AGG
             2300  CCTCAGG             
EcoNI         378  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1260  CCTGGAAGAGG         
KasI          363  GGCGCC               G^GCGCC
             1757  GGCGCC              
NaeI          424  GCCGGC               GCC^GGC
             1200  GCCGGC              
NarI          363  GGCGCC               GG^CGCC
             1757  GGCGCC              
NgoMIV        424  GCCGGC               G^CCGGC
             1200  GCCGGC              
PflMI        2094  CCACTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2754  CCAGATCCTGG         
PvuII         641  CAGCTG               CAG^CTG
             1762  CAGCTG              
SfoI          363  GGCGCC               GGC^GCC
             1757  GGCGCC              
SmaI         1670  CCCGGG               CCC^GGG
             2281  CCCGGG              
Van91I       2094  CCACTATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2754  CCAGATCCTGG         
XmaCI        1670  CCCGGG               C^CCGGG
             2281  CCCGGG              
XmaI         1670  CCCGGG               C^CCGGG
             2281  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     KspI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XcmI        
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