Restriction map for pF1KA0418
 (Linear) (Six-base) MAPSORT of: KIAA0418.seq  Check: 1308  from: 1  to: 2820



KIAA0418.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2013    2331    2820 
   Size:       2013     318     489

  Fragments arranged by size: 

                2013     489     318
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400    1229    1594    2820 
   Size:        400     829     365    1226

  Fragments arranged by size: 

                1226     829     400     365
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1317    1664    2820 
   Size:       1317     347    1156

  Fragments arranged by size: 

                1317    1156     347
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1126    2028    2760    2820 
   Size:       1126     902     732      60

  Fragments arranged by size: 

                1126     902     732      60
 
ApaI G_GGCC'C

Cuts at:      0     672     912    1033    1224    2088    2820 
   Size:        672     240     121     191     864     732

  Fragments arranged by size: 

                 864     732     672     240     191     121
 
ApoI r'AATT_y

Cuts at:      0     793    2820 
   Size:        793    2027
 
AvaI C'yCGr_G

Cuts at:      0     182    1670    2281    2820 
   Size:        182    1488     611     539

  Fragments arranged by size: 

                1488     611     539     182
 
BanI G'GyrC_C

Cuts at:      0     363     984    1757    2790    2820 
   Size:        363     621     773    1033      30

  Fragments arranged by size: 

                1033     773     621     363      30
 
BanII G_rGCy'C

Cuts at:      0     637     672     758     912    1033    1145    1224    1796 
   Size:        637      35      86     154     121     112      79     572

Cuts at:   1796    1804    2088    2820 
   Size:          8     284     732

  Fragments arranged by size: 

                 732     637     572     284     154     121     112      86
                  79      35       8
 
BbeI G_GCGC'C

Cuts at:      0     367    1761    2820 
   Size:        367    1394    1059

  Fragments arranged by size: 

                1394    1059     367
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1235    2820 
   Size:       1235    1585
 
BbvCI CC'TCA_GC

Cuts at:      0     662    1479    2820 
   Size:        662     817    1341

  Fragments arranged by size: 

                1341     817     662
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2509    2543    2820 
   Size:       2509      34     277

  Fragments arranged by size: 

                2509     277      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1191    1846    2450    2500    2524    2820 
   Size:       1191     655     604      50      24     296

  Fragments arranged by size: 

                1191     655     604     296      50      24
 
BlpI GC'TnA_GC

Cuts at:      0      58    1132    2820 
   Size:         58    1074    1688

  Fragments arranged by size: 

                1688    1074      58
 
Bme1580I G_kGCm'C

Cuts at:      0     672     912     989    1033    1224    2088    2159    2820 
   Size:        672     240      77      44     191     864      71     661

  Fragments arranged by size: 

                 864     672     661     240     191      77      71      44
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     424    1847    2352    2820 
   Size:        424    1423     505     468

  Fragments arranged by size: 

                1423     505     468     424
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     184     236     603     623    2050    2095    2218 
   Size:         49     135      52     367      20    1427      45     123

Cuts at:   2218    2294    2743    2820 
   Size:         76     449      77

  Fragments arranged by size: 

                1427     449     367     135     123      77      76      52
                  49      45      20
 
Bpu10I CC'TnA_GC

Cuts at:      0     662     949    1127    1479    2820 
   Size:        662     287     178     352    1341

  Fragments arranged by size: 

                1341     662     352     287     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1057    1417    1780    2820 
   Size:       1057     360     363    1040

  Fragments arranged by size: 

                1057    1040     363     360
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1264    1761    2820 
   Size:       1264     497    1059

  Fragments arranged by size: 

                1264    1059     497
 
BsaAI yAC'GTr

Cuts at:      0      18     882    2820 
   Size:         18     864    1938

  Fragments arranged by size: 

                1938     864      18
 
BsaBI GATnn'nnATC

Cuts at:      0    1741    2820 
   Size:       1741    1079
 
BsaHI Gr'CG_yC

Cuts at:      0     290     364     484    1758    2453    2820 
   Size:        290      74     120    1274     695     367

  Fragments arranged by size: 

                1274     695     367     290     120      74
 
BsaWI w'CCGG_w

Cuts at:      0     308    2820 
   Size:        308    2512
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     687     717    1075    1105    1333    1363    2032    2062 
   Size:        687      30     358      30     228      30     669      30

Cuts at:   2062    2692    2722    2820 
   Size:        630      30      98

  Fragments arranged by size: 

                 687     669     630     358     228      98      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     238    1039    1096    1153    1282    1323    1353 
   Size:        148      90     801      57      57     129      41      30

Cuts at:   1353    1467    1515    1518    1527    1530    1533    2457    2695 
   Size:        114      48       3       9       3       3     924     238

Cuts at:   2695    2820 
   Size:        125

  Fragments arranged by size: 

                 924     801     238     148     129     125     114      90
                  57      57      48      41      30       9       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     143     359     905    1003    1181    1651    1896    2081 
   Size:        143     216     546      98     178     470     245     185

Cuts at:   2081    2564    2786    2820 
   Size:        483     222      34

  Fragments arranged by size: 

                 546     483     470     245     222     216     185     178
                 143      98      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     904    2041    2820 
   Size:        262     642    1137     779

  Fragments arranged by size: 

                1137     779     642     262
 
BsiHKAI G_wGCw'C

Cuts at:      0     127    2820 
   Size:        127    2693
 
BsmI GAATG_Cn'

Cuts at:      0    1105    1452    2820 
   Size:       1105     347    1368

  Fragments arranged by size: 

                1368    1105     347
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2658    2820 
   Size:       2658     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     127     637     672     758     912     989    1033    1145 
   Size:        127     510      35      86     154      77      44     112

Cuts at:   1145    1224    1796    1804    2088    2159    2820 
   Size:         79     572       8     284      71     661

  Fragments arranged by size: 

                 661     572     510     284     154     127     112      86
                  79      77      71      44      35       8
 
BspEI T'CCGG_A

Cuts at:      0     308    2820 
   Size:        308    2512
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     407     476    2013    2331    2820 
   Size:        407      69    1537     318     489

  Fragments arranged by size: 

                1537     489     407     318      69
 
BsrBI CCG'CTC

Cuts at:      0     107    1583    2045    2820 
   Size:        107    1476     462     775

  Fragments arranged by size: 

                1476     775     462     107
 
BsrDI GCAATG_nn'

Cuts at:      0     536     579     785    2820 
   Size:        536      43     206    2035

  Fragments arranged by size: 

                2035     536     206      43
 
BsrFI r'CCGG_y

Cuts at:      0     424    1190    1200    2820 
   Size:        424     766      10    1620

  Fragments arranged by size: 

                1620     766     424      10
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     162    1786    2820 
   Size:        162    1624    1034

  Fragments arranged by size: 

                1624    1034     162
 
BstYI r'GATC_y

Cuts at:      0    2756    2820 
   Size:       2756      64
 
Bsu36I CC'TnA_GG

Cuts at:      0    1161    2301    2820 
   Size:       1161    1140     519

  Fragments arranged by size: 

                1161    1140     519
 
BtgI C'CryG_G

Cuts at:      0     593    1063    1145    1605    1785    2006    2711    2820 
   Size:        593     470      82     460     180     221     705     109

  Fragments arranged by size: 

                 705     593     470     460     221     180     109      82
            
 
BtsI GCAGTG_nn'

Cuts at:      0     594    2477    2820 
   Size:        594    1883     343

  Fragments arranged by size: 

                1883     594     343
 
ClaI AT'CG_AT

Cuts at:      0     926    2820 
   Size:        926    1894
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1545    1580    2820 
   Size:       1545      35    1240

  Fragments arranged by size: 

                1545    1240      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     613    2820 
   Size:        613    2207
 
EaeI y'GGCC_r

Cuts at:      0     422     647    1297    2432    2516    2820 
   Size:        422     225     650    1135      84     304

  Fragments arranged by size: 

                1135     650     422     304     225      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     215    1212    1259    1536    1551    2820 
   Size:        215     997      47     277      15    1269

  Fragments arranged by size: 

                1269     997     277     215      47      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     184     236     400     603     623    1229    1594 
   Size:         49     135      52     164     203      20     606     365

Cuts at:   1594    2050    2095    2218    2294    2743    2820 
   Size:        456      45     123      76     449      77

  Fragments arranged by size: 

                 606     456     449     365     203     164     135     123
                  77      76      52      49      45      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     382    1264    2820 
   Size:        382     882    1556

  Fragments arranged by size: 

                1556     882     382
 
EcoO109I rG'GnC_Cy

Cuts at:      0     147     531     909    1029    1030    2085    2354    2820 
   Size:        147     384     378     120       1    1055     269     466

  Fragments arranged by size: 

                1055     466     384     378     269     147     120       1
            
 
EcoRI G'AATT_C

Cuts at:      0     793    2820 
   Size:        793    2027
 
FseI GG_CCGG'CC

Cuts at:      0     428    2820 
   Size:        428    2392
 
HaeII r_GCGC'y

Cuts at:      0     367    1761    1970    2820 
   Size:        367    1394     209     850

  Fragments arranged by size: 

                1394     850     367     209
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     645     677     687     719     847     879    1144    1176 
   Size:        645      32      10      32     128      32     265      32

Cuts at:   1176    1303    1333    1335    1365    1650    1682    2692    2724 
   Size:        127      30       2      30     285      32    1010      32

Cuts at:   2724    2820 
   Size:         96

  Fragments arranged by size: 

                1010     645     285     265     128     127      96      32
                  32      32      32      32      32      30      30      10
                   2
 
HincII GTy'rAC

Cuts at:      0    2238    2820 
   Size:       2238     582
 
KasI G'GCGC_C

Cuts at:      0     363    1757    2820 
   Size:        363    1394    1063

  Fragments arranged by size: 

                1394    1063     363
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     218     759    1904    2481    2820 
   Size:         53     165     541    1145     577     339

  Fragments arranged by size: 

                1145     577     541     339     165      53
 
MscI TGG'CCA

Cuts at:      0     649    2820 
   Size:        649    2171
 
MslI CAynn'nnrTG

Cuts at:      0    2581    2820 
   Size:       2581     239
 
MspA1I CmG'CkG

Cuts at:      0     643     666    1065    1218    1607    1764    2008    2314 
   Size:        643      23     399     153     389     157     244     306

Cuts at:   2314    2491    2820 
   Size:        177     329

  Fragments arranged by size: 

                 643     399     389     329     306     244     177     157
                 153      23
 
NaeI GCC'GGC

Cuts at:      0     426    1202    2820 
   Size:        426     776    1618

  Fragments arranged by size: 

                1618     776     426
 
NarI GG'CG_CC

Cuts at:      0     364    1758    2820 
   Size:        364    1394    1062

  Fragments arranged by size: 

                1394    1062     364
 
NcoI C'CATG_G

Cuts at:      0    2711    2820 
   Size:       2711     109
 
NdeI CA'TA_TG

Cuts at:      0    1302    2820 
   Size:       1302    1518
 
NgoMIV G'CCGG_C

Cuts at:      0     424    1200    2820 
   Size:        424     776    1620

  Fragments arranged by size: 

                1620     776     424
 
NspI r_CATG'y

Cuts at:      0    1964    2820 
   Size:       1964     856
 
PasI CC'CwG_GG

Cuts at:      0     630    2820 
   Size:        630    2190
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2100    2760    2820 
   Size:       2100     660      60

  Fragments arranged by size: 

                2100     660      60
 
PfoI T'CCnGG_A

Cuts at:      0       5    1982    2696    2759    2820 
   Size:          5    1977     714      63      61

  Fragments arranged by size: 

                1977     714      63      61       5
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1462    1494    2820 
   Size:       1462      32    1326

  Fragments arranged by size: 

                1462    1326      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     147    2820 
   Size:        147    2673
 
PspOMI G'GGCC_C

Cuts at:      0     668     908    1029    1220    2084    2820 
   Size:        668     240     121     191     864     736

  Fragments arranged by size: 

                 864     736     668     240     191     121
 
PstI C_TGCA'G

Cuts at:      0    1217    2820 
   Size:       1217    1603
 
PvuII CAG'CTG

Cuts at:      0     643    1764    2820 
   Size:        643    1121    1056

  Fragments arranged by size: 

                1121    1056     643
 
RsrII CG'GwC_CG

Cuts at:      0    1686    2820 
   Size:       1686    1134
 
SacII CC_GC'GG

Cuts at:      0    1066    1608    2009    2820 
   Size:       1066     542     401     811

  Fragments arranged by size: 

                1066     811     542     401
 
SanDI GG'GwC_CC

Cuts at:      0     147    2820 
   Size:        147    2673
 
ScaI AGT'ACT

Cuts at:      0     778    2820 
   Size:        778    2042
 
SfcI C'TryA_G

Cuts at:      0    1213    2820 
   Size:       1213    1607
 
SfoI GGC'GCC

Cuts at:      0     365    1759    2820 
   Size:        365    1394    1061

  Fragments arranged by size: 

                1394    1061     365
 
SmaI CCC'GGG

Cuts at:      0    1672    2283    2820 
   Size:       1672     611     537

  Fragments arranged by size: 

                1672     611     537
 
SmlI C'TyrA_G

Cuts at:      0    1072    1432    1795    2806    2820 
   Size:       1072     360     363    1011      14

  Fragments arranged by size: 

                1072    1011     363     360      14
 
StyI C'CwwG_G

Cuts at:      0     389     980    1013    1616    2162    2384    2504    2711 
   Size:        389     591      33     603     546     222     120     207

Cuts at:   2711    2820 
   Size:        109

  Fragments arranged by size: 

                 603     591     546     389     222     207     120     109
                  33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1225    1674    2397    2778    2820 
   Size:       1225     449     723     381      42

  Fragments arranged by size: 

                1225     723     449     381      42
 
TatI w'GTAC_w

Cuts at:      0     776    2820 
   Size:        776    2044
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     313     345    2032    2064    2820 
   Size:        313      32    1687      32     756

  Fragments arranged by size: 

                1687     756     313      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2511    2718    2761    2820 
   Size:       2511     207      43      59

  Fragments arranged by size: 

                2511     207      59      43
 
XmaI C'CCGG_G

Cuts at:      0    1670    2281    2820 
   Size:       1670     611     539

  Fragments arranged by size: 

                1670     611     539
 
XmnI GAAnn'nnTTC

Cuts at:      0    2776    2820 
   Size:       2776      44
 

 Enzymes that do cut:

     AarI     AcuI     AhdI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BstXI    BstYI
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DrdI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FseI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI     PpiI    PpuMI
   PspOMI     PstI    PvuII    RsrII    SacII    SanDI     ScaI     SfcI
     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI
     BplI    BsiEI    BsiWI    BspHI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I    BtgZI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoRV     FalI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI     SacI
     SalI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     ZraI
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