Restriction map for pF1KA0417
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflIII      
AgeI        AhdI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BalI        BbrPI       BbvCI       BcgI        
BclI        BfaI        BglII       BlnI        BsaI        
BseAI       BseRI       BsiWI       Bsp1407I    BspEI       
BspLU11I    BspMI       BsrGI       BssHII      Bst1107I    
BstXI       BstZ17I     CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRI       EcoRV       FbaI        FseI        FspI        
HgaI        HindIII     HpaI        KpnI        MaeI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NdeI        NheI        NotI        NruI        
NspI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SapI        SbfI        
SexAI       SfiI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        TfiI        
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII        1696  GACGTC               GACGT^C
AflII        1003  CTTAAG               C^TTAAG
Alw44I       1689  GTGCAC               G^TGCAC
AocI          715  CCTGAGG              CC^T[ACGT]AGG
ApaI           31  GGGCCC               GGGCC^C
ApaLI        1689  GTGCAC               G^TGCAC
Asp700I      1066  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1822  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         375  GGATCC               G^GATCC
BanII          31  GGGCCC               G[GA]GC[CT]^C
BbeI         1458  GGCGCC               GGCGC^C
BbsI          291  GAAGAC               GAAGAC(2/6)
BciVI         453  GGATAC               GTATCC(6/5)
BfrI         1003  CTTAAG               C^TTAAG
BglI          655  GCCTACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          768  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      768  GCTGAGC              GC^T[ACGT]AGC
BpuAI         291  GAAGAC               GAAGAC(2/6)
BsaAI        1633  TACGTG               [CT]AC^GT[GA]
BsaBI        1509  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1594  CGGTCG               CG[GA][CT]^CG
BsmBI         144  CGTCTC               CGTCTC(1/5)
BsmI          235  GAATGC               GAATGC(1/-1)
BspDI        1366  ATCGAT               AT^CGAT
BspHI         641  TCATGA               T^CATGA
BsrBI        1600  CCGCTC               CCGCTC(-3/-3)
BssSI         166  CTCGTG               CACGAG(-5/-1)
Bsu36I        715  CCTGAGG              CC^T[ACGT]AGG
CelII         768  GCTGAGC              GC^T[ACGT]AGC
ClaI         1366  ATCGAT               AT^CGAT
EarI         1989  GAAGAG               CTCTTC(1/4)
Eco81I        715  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       237  ATGCAT               ATGCA^T
KasI         1458  GGCGCC               G^GCGCC
Ksp632I      1989  GAAGAG               CTCTTC(1/4)
MamI         1509  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         1458  GGCGCC               GG^CGCC
NcoI          449  CCATGG               C^CATGG
NsiI          237  ATGCAT               ATGCA^T
PflFI        1822  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII        1783  CAGCTG               CAG^CTG
RcaI          641  TCATGA               T^CATGA
SalI          181  GTCGAC               G^TCGAC
ScaI          521  AGTACT               AGT^ACT
SfoI         1458  GGCGCC               GGC^GCC
SmaI         1846  CCCGGG               CCC^GGG
SspI         1083  AATATT               AAT^ATT
StuI          666  AGGCCT               AGG^CCT
Tth111I      1822  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1846  CCCGGG               C^CCGGG
XmaI         1846  CCCGGG               C^CCGGG
XmnI         1066  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          181  GTCGAC               GT^[AC][GT]AC
              961  GTAGAC              
AccII        1249  CGCG                 CG^CG
             1575  CGCG                
AcyI         1458  GGCGCC               G[GA]^CG[CT]C
             1696  GACGTC              
AlwNI         394  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              535  CAGGCGCTG           
BsaHI        1458  GGCGCC               G[GA]^CG[CT]C
             1696  GACGTC              
BsaWI         983  TCCGGT               [AT]^CCGG[AT]
              990  ACCGGA              
BsgI          427  CTGCAC               GTGCAG(16/14)
             1480  GTGCAG              
BsrDI         702  CATTGC               GCAATG(2/0)
             1283  GCAATG              
BstBI        1063  TTCGAA               TT^CGAA
             1986  TTCGAA              
BstEII        268  GGTGACC              G^GT[ACGT]ACC
              986  GGTTACC             
BstPI         268  GGTGACC              G^GT[ACGT]ACC
              986  GGTTACC             
BstUI        1249  CGCG                 CG^CG
             1575  CGCG                
DraIII       1255  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1680  CACTCGGTG           
EcoO65I       268  GGTGACC              G^GT[ACGT]ACC
              986  GGTTACC             
HaeII         537  GGCGCT               [GA]GCGC^[CT]
             1458  GGCGCC              
Hin1I        1458  GGCGCC               G[GA]^CG[CT]C
             1696  GACGTC              
HincII        181  GTCGAC               GT[CT]^[GA]AC
             1138  GTTGAC              
HindII        181  GTCGAC               GT[CT]^[GA]AC
             1138  GTTGAC              
KspI         1248  CCGCGG               CCGC^GG
             1574  CCGCGG              
MvnI         1249  CGCG                 CG^CG
             1575  CGCG                
NaeI           16  GCCGGC               GCC^GGC
              627  GCCGGC              
NgoMIV         16  GCCGGC               G^CCGGC
              627  GCCGGC              
NspV         1063  TTCGAA               TT^CGAA
             1986  TTCGAA              
PpuMI         440  GGGACCT              [GA]G^G[AT]CC[CT]
             1391  GGGACCT             
SacII        1248  CCGCGG               CCGC^GG
             1574  CCGCGG              
SfuI         1063  TTCGAA               TT^CGAA
             1986  TTCGAA              
SmlI         1003  CTTAAG               C^T[CT][GA]AG
             1543  CTCAAG              
Tsp509I      1109  AATT                 ^AATT
             2020  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaI        AvaII       BanI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XhoII       
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