Restriction map for pF1KA0417
 (Linear) (Six-base) MAPSORT of: KIAA0417.seq  Check: 8739  from: 1  to: 2025



KIAA0417.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AatII G_ACGT'C

Cuts at:      0    1700    2025 
   Size:       1700     325
 
AccI GT'mk_AC

Cuts at:      0     182     962    2025 
   Size:        182     780    1063

  Fragments arranged by size: 

                1063     780     182
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562    2025 
   Size:        562    1463
 
AflII C'TTAA_G

Cuts at:      0    1003    2025 
   Size:       1003    1022
 
AleI CACnn'nnGTG

Cuts at:      0    1903    2025 
   Size:       1903     122
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1921    2025 
   Size:       1921     104
 
AlwNI CAG_nnn'CTG

Cuts at:      0     399     540    2025 
   Size:        399     141    1485

  Fragments arranged by size: 

                1485     399     141
 
ApaI G_GGCC'C

Cuts at:      0      35    2025 
   Size:         35    1990
 
ApaLI G'TGCA_C

Cuts at:      0    1689    2025 
   Size:       1689     336
 
AvaI C'yCGr_G

Cuts at:      0      35     319     679    1408    1846    2025 
   Size:         35     284     360     729     438     179

  Fragments arranged by size: 

                 729     438     360     284     179      35
 
BamHI G'GATC_C

Cuts at:      0     375    2025 
   Size:        375    1650
 
BanI G'GyrC_C

Cuts at:      0     606     955    1458    1549    1905    2025 
   Size:        606     349     503      91     356     120

  Fragments arranged by size: 

                 606     503     356     349     120      91
 
BanII G_rGCy'C

Cuts at:      0      35    2025 
   Size:         35    1990
 
BbeI G_GCGC'C

Cuts at:      0    1462    2025 
   Size:       1462     563
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     298    2025 
   Size:        298    1727
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     447    2025 
   Size:        447    1578
 
BfrBI ATG'CAT

Cuts at:      0     239    2025 
   Size:        239    1786
 
BglI GCCn_nnn'nGGC

Cuts at:      0     661    2025 
   Size:        661    1364
 
BlpI GC'TnA_GC

Cuts at:      0     769    2025 
   Size:        769    1256
 
Bme1580I G_kGCm'C

Cuts at:      0      35    1256    1693    1910    2025 
   Size:         35    1221     437     217     115

  Fragments arranged by size: 

                1221     437     217     115      35
 
BmgBI CAC'GTC

Cuts at:      0    1527    2025 
   Size:       1527     498
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     446    2025 
   Size:        446    1579
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     741    2025 
   Size:        741    1284
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     427     934    2025 
   Size:         53     374     507    1091

  Fragments arranged by size: 

                1091     507     374      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     836    2025 
   Size:        836    1189
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1528    2025 
   Size:       1528     497
 
BsaAI yAC'GTr

Cuts at:      0    1635    2025 
   Size:       1635     390
 
BsaBI GATnn'nnATC

Cuts at:      0    1513    2025 
   Size:       1513     512
 
BsaHI Gr'CG_yC

Cuts at:      0    1459    1697    2025 
   Size:       1459     238     328

  Fragments arranged by size: 

                1459     328     238
 
BsaWI w'CCGG_w

Cuts at:      0     983     990    2025 
   Size:        983       7    1035

  Fragments arranged by size: 

                1035     983       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     121     151    2025 
   Size:        121      30    1874

  Fragments arranged by size: 

                1874     121      30
 
BseYI C'CCAG_C

Cuts at:      0     820    1781    2025 
   Size:        820     961     244

  Fragments arranged by size: 

                 961     820     244
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1501    2025 
   Size:        412    1089     524

  Fragments arranged by size: 

                1089     524     412
 
BsiEI CG_ry'CG

Cuts at:      0    1597    2025 
   Size:       1597     428
 
BsiHKAI G_wGCw'C

Cuts at:      0    1268    1693    1820    2025 
   Size:       1268     425     127     205

  Fragments arranged by size: 

                1268     425     205     127
 
BsmI GAATG_Cn'

Cuts at:      0     241    2025 
   Size:        241    1784
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     150    2025 
   Size:        150    1875
 
Bsp1286I G_dGCh'C

Cuts at:      0      35    1256    1268    1693    1820    1910    2025 
   Size:         35    1221      12     425     127      90     115

  Fragments arranged by size: 

                1221     425     127     115      90      35      12
 
BspHI T'CATG_A

Cuts at:      0     641    2025 
   Size:        641    1384
 
BsrBI CCG'CTC

Cuts at:      0    1602    2025 
   Size:       1602     423
 
BsrDI GCAATG_nn'

Cuts at:      0     701    1290    2025 
   Size:        701     589     735

  Fragments arranged by size: 

                 735     701     589
 
BsrFI r'CCGG_y

Cuts at:      0      16     627    1592    2025 
   Size:         16     611     965     433

  Fragments arranged by size: 

                 965     611     433      16
 
BssSI C'ACGA_G

Cuts at:      0     166    2025 
   Size:        166    1859
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1272    1317    1479    2025 
   Size:       1272      45     162     546

  Fragments arranged by size: 

                1272     546     162      45
 
BstBI TT'CG_AA

Cuts at:      0    1064    1987    2025 
   Size:       1064     923      38

  Fragments arranged by size: 

                1064     923      38
 
BstEII G'GTnAC_C

Cuts at:      0     268     986    2025 
   Size:        268     718    1039

  Fragments arranged by size: 

                1039     718     268
 
BstYI r'GATC_y

Cuts at:      0     375     980    1039    1878    1922    2025 
   Size:        375     605      59     839      44     103

  Fragments arranged by size: 

                 839     605     375     103      59      44
 
Bsu36I CC'TnA_GG

Cuts at:      0     716    2025 
   Size:        716    1309
 
BtgI C'CryG_G

Cuts at:      0     449    1248    1574    2025 
   Size:        449     799     326     451

  Fragments arranged by size: 

                 799     451     449     326
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     270    2025 
   Size:        270    1755
 
BtsI GCAGTG_nn'

Cuts at:      0     400     810    1898    2025 
   Size:        400     410    1088     127

  Fragments arranged by size: 

                1088     410     400     127
 
ClaI AT'CG_AT

Cuts at:      0    1367    2025 
   Size:       1367     658
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1953    1988    2025 
   Size:       1953      35      37

  Fragments arranged by size: 

                1953      37      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1260    1685    2025 
   Size:       1260     425     340

  Fragments arranged by size: 

                1260     425     340
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     142     970    1576    2025 
   Size:        142     828     606     449

  Fragments arranged by size: 

                 828     606     449     142
 
EaeI y'GGCC_r

Cuts at:      0     176     629    1769    2025 
   Size:        176     453    1140     256

  Fragments arranged by size: 

                1140     453     256     176
 
EarI CTCTTCn'nnn_

Cuts at:      0    1984    2025 
   Size:       1984      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1043    1861    2025 
   Size:         19    1024     818     164

  Fragments arranged by size: 

                1024     818     164      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     427     562     934    2025 
   Size:         53     374     135     372    1091

  Fragments arranged by size: 

                1091     374     372     135      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32     441    1392    2025 
   Size:         32     409     951     633

  Fragments arranged by size: 

                 951     633     409      32
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1449    2025 
   Size:       1449     576
 
HaeII r_GCGC'y

Cuts at:      0     541    1462    2025 
   Size:        541     921     563

  Fragments arranged by size: 

                 921     563     541
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     584     616     960     992    2025 
   Size:        121      32     431      32     344      32    1033

  Fragments arranged by size: 

                1033     431     344     121      32      32      32
 
HincII GTy'rAC

Cuts at:      0     183    1140    2025 
   Size:        183     957     885

  Fragments arranged by size: 

                 957     885     183
 
KasI G'GCGC_C

Cuts at:      0    1458    2025 
   Size:       1458     567
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     322     847    2025 
   Size:        164     158     525    1178

  Fragments arranged by size: 

                1178     525     164     158
 
MslI CAynn'nnrTG

Cuts at:      0     244     604    1585    1903    2025 
   Size:        244     360     981     318     122

  Fragments arranged by size: 

                 981     360     318     244     122
 
MspA1I CmG'CkG

Cuts at:      0     800    1200    1250    1576    1785    2025 
   Size:        800     400      50     326     209     240

  Fragments arranged by size: 

                 800     400     326     240     209      50
 
NaeI GCC'GGC

Cuts at:      0      18     629    2025 
   Size:         18     611    1396

  Fragments arranged by size: 

                1396     611      18
 
NarI GG'CG_CC

Cuts at:      0    1459    2025 
   Size:       1459     566
 
NcoI C'CATG_G

Cuts at:      0     449    2025 
   Size:        449    1576
 
NgoMIV G'CCGG_C

Cuts at:      0      16     627    2025 
   Size:         16     611    1398

  Fragments arranged by size: 

                1398     611      16
 
NsiI A_TGCA'T

Cuts at:      0     241    2025 
   Size:        241    1784
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     372     395    1527    1731    2025 
   Size:        372      23    1132     204     294

  Fragments arranged by size: 

                1132     372     294     204      23
 
PfoI T'CCnGG_A

Cuts at:      0    1388    2025 
   Size:       1388     637
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     313     345    2025 
   Size:        313      32    1680

  Fragments arranged by size: 

                1680     313      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     441    1392    2025 
   Size:        441     951     633

  Fragments arranged by size: 

                 951     633     441
 
PspOMI G'GGCC_C

Cuts at:      0      31    2025 
   Size:         31    1994
 
PstI C_TGCA'G

Cuts at:      0     521    1131    1472    2025 
   Size:        521     610     341     553

  Fragments arranged by size: 

                 610     553     521     341
 
PvuII CAG'CTG

Cuts at:      0    1785    2025 
   Size:       1785     240
 
SacII CC_GC'GG

Cuts at:      0    1251    1577    2025 
   Size:       1251     326     448

  Fragments arranged by size: 

                1251     448     326
 
SalI G'TCGA_C

Cuts at:      0     181    2025 
   Size:        181    1844
 
ScaI AGT'ACT

Cuts at:      0     523    2025 
   Size:        523    1502
 
SfcI C'TryA_G

Cuts at:      0     213     517    1127    1468    1806    2025 
   Size:        213     304     610     341     338     219

  Fragments arranged by size: 

                 610     341     338     304     219     213
 
SfoI GGC'GCC

Cuts at:      0    1460    2025 
   Size:       1460     565
 
SmaI CCC'GGG

Cuts at:      0    1848    2025 
   Size:       1848     177
 
SmlI C'TyrA_G

Cuts at:      0    1003    1543    2025 
   Size:       1003     540     482

  Fragments arranged by size: 

                1003     540     482
 
SspI AAT'ATT

Cuts at:      0    1085    2025 
   Size:       1085     940
 
StuI AGG'CCT

Cuts at:      0     668    2025 
   Size:        668    1357
 
StyI C'CwwG_G

Cuts at:      0     224     449     707    2025 
   Size:        224     225     258    1318

  Fragments arranged by size: 

                1318     258     225     224
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1729    2025 
   Size:       1729     296
 
TatI w'GTAC_w

Cuts at:      0     521    2025 
   Size:        521    1504
 
Tth111I GACn'n_nGTC

Cuts at:      0    1825    2025 
   Size:       1825     200
 
XmaI C'CCGG_G

Cuts at:      0    1846    2025 
   Size:       1846     179
 
XmnI GAAnn'nnTTC

Cuts at:      0    1070    2025 
   Size:       1070     955
 
ZraI GAC'GTC

Cuts at:      0    1698    2025 
   Size:       1698     327
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BciVI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII
     KasI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI    PflMI     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII
    SacII     SalI     ScaI     SfcI     SfoI     SmaI     SmlI     SspI
     StuI     StyI    TaqII     TatI  Tth111I     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI
     BclI    BglII     BmtI     BsaI    BseRI    BsiWI    BspEI    BspMI
    BsrGI   BssHII    BstXI  BstZ17I     DraI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     NdeI     NheI     NotI     NruI     NspI
     PacI     PasI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SanDI     SapI     SbfI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SwaI
     TstI     XbaI     XcmI     XhoI
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