Restriction map for pF1KA0369
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglII       BlnI        BseAI       BsgI        
BsiEI       BsiWI       BsmI        BspEI       BspHI       
BspLU11I    BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstYI       BstZ17I     Bsu36I      CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoT14I     EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MflI        MluI        
MroI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tth111I     XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI         1618  GTAGAC               GT^[AC][GT]AC
AcyI         1459  GACGCC               G[GA]^CG[CT]C
AflIII        704  ACACGT               A^C[GA][CT]GT
AgeI          236  ACCGGT               A^CCGGT
AlwI         1296  GATCC                GGATC(4/5)
BanI         1645  GGCACC               G^G[CT][GA]CC
BciVI        1687  GGATAC               GTATCC(6/5)
BclI         1794  TGATCA               T^GATCA
BglI          256  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          147  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      147  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1657  TACGTG               [CT]AC^GT[GA]
BsaBI        1891  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1459  GACGCC               G[GA]^CG[CT]C
BsaI         1412  GAGACC               GGTCTC(1/5)
BsmBI        1457  GAGACG               CGTCTC(1/5)
BsmFI         918  GTCCC                GGGAC(10/14)
BspDI         197  ATCGAT               AT^CGAT
BsrDI        1674  CATTGC               GCAATG(2/0)
BsrFI         236  ACCGGT               [GA]^CCGG[CT]
BstEII        561  GGTCACC              G^GT[ACGT]ACC
BstPI         561  GGTCACC              G^GT[ACGT]ACC
BstXI         668  CCATCAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         147  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        236  ACCGGT               [GA]^CCGG[CT]
ClaI          197  ATCGAT               AT^CGAT
EarI         1322  GAAGAG               CTCTTC(1/4)
EcoO65I       561  GGTCACC              G^GT[ACGT]ACC
FbaI         1794  TGATCA               T^GATCA
Hin1I        1459  GACGCC               G[GA]^CG[CT]C
Ksp632I      1322  GAAGAG               CTCTTC(1/4)
MamI         1891  GATGTAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI          769  GCATGT               [GA]CATG^[CT]
PinAI         236  ACCGGT               A^CCGGT
PleI          679  GACTC                GAGTC(4/5)
PpuMI         644  AGGTCCT              [GA]G^G[AT]CC[CT]
ScaI         1920  AGTACT               AGT^ACT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          74  CGCG                 CG^CG
              974  CGCG                
AcsI          297  GAATTT               [GA]^AATT[CT]
             1197  AAATTT              
ApoI          297  GAATTT               [GA]^AATT[CT]
             1197  AAATTT              
Asp700I       512  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1998  GAAGCATTTC          
AvaI          479  CTCGGG               C^[CT]CG[GA]G
              681  CTCGGG              
BalI          497  TGGCCA               TGG^CCA
             1607  TGGCCA              
BbsI         2170  GAAGAC               GAAGAC(2/6)
              489  GTCTTC              
BpuAI        2170  GAAGAC               GAAGAC(2/6)
              489  GTCTTC              
BsaWI         236  ACCGGT               [AT]^CCGG[AT]
              777  ACCGGA              
BsoBI         479  CTCGGG               C^[CT]CG[GA]G
              681  CTCGGG              
BsrBI        2137  CCGCTC               CCGCTC(-3/-3)
               40  GAGCGG              
BstUI          74  CGCG                 CG^CG
              974  CGCG                
EaeI          497  TGGCCA               [CT]^GGCC[GA]
             1607  TGGCCA              
EcoRV         655  GATATC               GAT^ATC
             1531  GATATC              
HaeII         106  AGCGCC               [GA]GCGC^[CT]
             1489  AGCGCC              
HapII         237  CCGG                 C^CGG
              778  CCGG                
HpaII         237  CCGG                 C^CGG
              778  CCGG                
MluNI         497  TGGCCA               TGG^CCA
             1607  TGGCCA              
MscI          497  TGGCCA               TGG^CCA
             1607  TGGCCA              
MslI         1564  CACCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1869  CATTACCATG          
MspA1I       1972  CAGCTG               C[AC]G^C[GT]G
             2036  CAGCTG              
MspI          237  CCGG                 C^CGG
              778  CCGG                
MvnI           74  CGCG                 CG^CG
              974  CGCG                
PflMI         363  CCAACTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1756  CCATTCCGTGG         
PvuII        1972  CAGCTG               CAG^CTG
             2036  CAGCTG              
SacI         1035  GAGCTC               GAGCT^C
             1864  GAGCTC              
Van91I        363  CCAACTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1756  CCATTCCGTGG         
XcmI         1874  CCATGATGCTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2113  CCAGGAATGTATTGG     
XmnI          512  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1998  GAAGCATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaII       BanII       BbvI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    Bsp1407I    BspMI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HgaI        HhaI        
HinP1I      HinfI       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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