Restriction map for pF1KA0369
 (Linear) (Six-base) MAPSORT of: KIAA0369.seq  Check: 2843  from: 1  to: 2187



KIAA0369.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1708    1803    2187 
   Size:       1708      95     384

  Fragments arranged by size: 

                1708     384      95
 
AccI GT'mk_AC

Cuts at:      0    1619    2187 
   Size:       1619     568
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     718    1606    2187 
   Size:        503     215     888     581

  Fragments arranged by size: 

                 888     581     503     215
 
AflIII A'CryG_T

Cuts at:      0     704    2187 
   Size:        704    1483
 
AgeI A'CCGG_T

Cuts at:      0     236    2187 
   Size:        236    1951
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1684    1984    2038    2187 
   Size:       1684     300      54     149

  Fragments arranged by size: 

                1684     300     149      54
 
ApoI r'AATT_y

Cuts at:      0     297    1197    2187 
   Size:        297     900     990

  Fragments arranged by size: 

                 990     900     297
 
AvaI C'yCGr_G

Cuts at:      0     479     681    2187 
   Size:        479     202    1506

  Fragments arranged by size: 

                1506     479     202
 
BanI G'GyrC_C

Cuts at:      0    1645    2187 
   Size:       1645     542
 
BanII G_rGCy'C

Cuts at:      0      97     341     407     895    1039    1868    2187 
   Size:         97     244      66     488     144     829     319

  Fragments arranged by size: 

                 829     488     319     244     144      97      66
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     482    2177    2187 
   Size:        482    1695      10

  Fragments arranged by size: 

                1695     482      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1681    2187 
   Size:       1681     506
 
BclI T'GATC_A

Cuts at:      0    1794    2187 
   Size:       1794     393
 
BglI GCCn_nnn'nGGC

Cuts at:      0     262    2187 
   Size:        262    1925
 
BlpI GC'TnA_GC

Cuts at:      0     148    2187 
   Size:        148    2039
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1845    2187 
   Size:       1845     342
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1055    2187 
   Size:       1055    1132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     439    1133    1649    2060    2187 
   Size:         46     393     694     516     411     127

  Fragments arranged by size: 

                 694     516     411     393     127      46
 
Bpu10I CC'TnA_GC

Cuts at:      0     978    2187 
   Size:        978    1209
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325    1681    1945    2187 
   Size:        325    1356     264     242

  Fragments arranged by size: 

                1356     325     264     242
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1406    2187 
   Size:       1406     781
 
BsaAI yAC'GTr

Cuts at:      0    1659    2187 
   Size:       1659     528
 
BsaBI GATnn'nnATC

Cuts at:      0    1895    2187 
   Size:       1895     292
 
BsaHI Gr'CG_yC

Cuts at:      0    1460    2187 
   Size:       1460     727
 
BsaWI w'CCGG_w

Cuts at:      0     236     777    2187 
   Size:        236     541    1410

  Fragments arranged by size: 

                1410     541     236
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1106    1136    2033    2063    2187 
   Size:       1106      30     897      30     124

  Fragments arranged by size: 

                1106     897     124      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1044    1047    1372    2187 
   Size:       1044       3     325     815

  Fragments arranged by size: 

                1044     815     325       3
 
BseYI C'CCAG_C

Cuts at:      0    1590    2187 
   Size:       1590     597
 
BsiHKAI G_wGCw'C

Cuts at:      0      32     883    1039    1292    1565    1868    2187 
   Size:         32     851     156     253     273     303     319

  Fragments arranged by size: 

                 851     319     303     273     253     156      32
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1451    2187 
   Size:       1451     736
 
Bsp1286I G_dGCh'C

Cuts at:      0      32      97     341     407     883     895    1039    1292 
   Size:         32      65     244      66     476      12     144     253

Cuts at:   1292    1565    1868    2187 
   Size:        273     303     319

  Fragments arranged by size: 

                 476     319     303     273     253     244     144      66
                  65      32      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     633    1511    1556    1708    1803    2147    2187 
   Size:        633     878      45     152      95     344      40

  Fragments arranged by size: 

                 878     633     344     152      95      45      40
 
BsrBI CCG'CTC

Cuts at:      0      42    2139    2187 
   Size:         42    2097      48

  Fragments arranged by size: 

                2097      48      42
 
BsrDI GCAATG_nn'

Cuts at:      0    1673    2187 
   Size:       1673     514
 
BsrFI r'CCGG_y

Cuts at:      0     236    2187 
   Size:        236    1951
 
BsrGI T'GTAC_A

Cuts at:      0     701    1475    1631    2187 
   Size:        701     774     156     556

  Fragments arranged by size: 

                 774     701     556     156
 
BstEII G'GTnAC_C

Cuts at:      0     561    2187 
   Size:        561    1626
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     675    2187 
   Size:        675    1512
 
BtgI C'CryG_G

Cuts at:      0     589    1761    2064    2187 
   Size:        589    1172     303     123

  Fragments arranged by size: 

                1172     589     303     123
 
BtsI GCAGTG_nn'

Cuts at:      0     111     491     500    2187 
   Size:        111     380       9    1687

  Fragments arranged by size: 

                1687     380     111       9
 
ClaI AT'CG_AT

Cuts at:      0     198    2187 
   Size:        198    1989
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1640    1675    2123    2158    2187 
   Size:       1640      35     448      35      29

  Fragments arranged by size: 

                1640     448      35      35      29
 
EaeI y'GGCC_r

Cuts at:      0     497    1607    2187 
   Size:        497    1110     580

  Fragments arranged by size: 

                1110     580     497
 
EarI CTCTTCn'nnn_

Cuts at:      0    1317    2187 
   Size:       1317     870
 
EcoICRI GAG'CTC

Cuts at:      0    1037    1866    2187 
   Size:       1037     829     321

  Fragments arranged by size: 

                1037     829     321
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     439     503     718    1133    1606    1649    2060 
   Size:         46     393      64     215     415     473      43     411

Cuts at:   2060    2187 
   Size:        127

  Fragments arranged by size: 

                 473     415     411     393     215     127      64      46
                  43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     645    2014    2187 
   Size:        255     390    1369     173

  Fragments arranged by size: 

                1369     390     255     173
 
EcoRV GAT'ATC

Cuts at:      0     657    1533    2187 
   Size:        657     876     654

  Fragments arranged by size: 

                 876     657     654
 
HaeII r_GCGC'y

Cuts at:      0     110    1493    2187 
   Size:        110    1383     694

  Fragments arranged by size: 

                1383     694     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     264     296    1157    1189    1679    1711    2033    2065 
   Size:        264      32     861      32     490      32     322      32

Cuts at:   2065    2123    2155    2187 
   Size:         58      32      32

  Fragments arranged by size: 

                 861     490     322     264      58      32      32      32
                  32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     689    1106    1155    2187 
   Size:        266     423     417      49    1032

  Fragments arranged by size: 

                1032     423     417     266      49
 
MscI TGG'CCA

Cuts at:      0     499    1609    2187 
   Size:        499    1110     578

  Fragments arranged by size: 

                1110     578     499
 
MslI CAynn'nnrTG

Cuts at:      0    1568    1873    2187 
   Size:       1568     305     314

  Fragments arranged by size: 

                1568     314     305
 
MspA1I CmG'CkG

Cuts at:      0    1974    2038    2187 
   Size:       1974      64     149

  Fragments arranged by size: 

                1974     149      64
 
NspI r_CATG'y

Cuts at:      0     773    2187 
   Size:        773    1414
 
PasI CC'CwG_GG

Cuts at:      0     306    1934    2187 
   Size:        306    1628     253

  Fragments arranged by size: 

                1628     306     253
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     369    1762    2187 
   Size:        369    1393     425

  Fragments arranged by size: 

                1393     425     369
 
PpuMI rG'GwC_Cy

Cuts at:      0     645    2187 
   Size:        645    1542
 
PstI C_TGCA'G

Cuts at:      0     118     143    1087    2187 
   Size:        118      25     944    1100

  Fragments arranged by size: 

                1100     944     118      25
 
PvuII CAG'CTG

Cuts at:      0    1974    2038    2187 
   Size:       1974      64     149

  Fragments arranged by size: 

                1974     149      64
 
SacI G_AGCT'C

Cuts at:      0    1039    1868    2187 
   Size:       1039     829     319

  Fragments arranged by size: 

                1039     829     319
 
ScaI AGT'ACT

Cuts at:      0    1922    2187 
   Size:       1922     265
 
SfcI C'TryA_G

Cuts at:      0     114     139    1083    2187 
   Size:        114      25     944    1104

  Fragments arranged by size: 

                1104     944     114      25
 
SmlI C'TyrA_G

Cuts at:      0     340    1696    1924    2187 
   Size:        340    1356     228     263

  Fragments arranged by size: 

                1356     340     263     228
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1017    1582    2187 
   Size:       1017     565     605

  Fragments arranged by size: 

                1017     605     565
 
TatI w'GTAC_w

Cuts at:      0     422     701    1475    1631    1920    2187 
   Size:        422     279     774     156     289     267

  Fragments arranged by size: 

                 774     422     289     279     267     156
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1106    1138    2187 
   Size:       1106      32    1049

  Fragments arranged by size: 

                1106    1049      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1881    2120    2187 
   Size:       1881     239      67

  Fragments arranged by size: 

                1881     239      67
 
XmnI GAAnn'nnTTC

Cuts at:      0     516    2002    2187 
   Size:        516    1486     185

  Fragments arranged by size: 

                1486     516     185
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI    AlwNI     ApoI     AvaI
     BanI    BanII     BbsI    BciVI     BclI     BglI     BlpI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BstEII    BstXI     BtgI     BtsI
     ClaI    CspCI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRV
    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I     NspI     PasI
    PflMI    PpuMI     PstI    PvuII     SacI     ScaI     SfcI     SmlI
    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BfrBI    BglII Bme1580I    BmgBI
     BmtI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI   BssHII
    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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