Restriction map for pF1KA0363
 (Circular) (Six-base) MAPSORT of: pF1KA0363.seq  Check: 1700  from: 1  to: 7520



pF1KA0363  7520 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:19  ..

AccI GT'mk_AC

Cuts at:   4558    4558 
   Size:       7520
 
Acc65I G'GTAC_C

Cuts at:   3526    4536    3526 
   Size:       1010    6510
 
AclI AA'CG_TT

Cuts at:   7360    7360 
   Size:       7520
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    575    1739    1859    1979    2099    2219    2339    2459    2579 
   Size:       1164     120     120     120     120     120     120     120

Cuts at:   2579    3100    3278    5345    5777    6130    6792     575 
   Size:        521     178    2067     432     353     662    1303

  Fragments arranged by size: 

                2067    1303    1164     662     521     432     353     178
                 120     120     120     120     120     120     120
 
AfeI AGC'GCT

Cuts at:    242     242 
   Size:       7520
 
AflIII A'CryG_T

Cuts at:   4872    6672    6953    4872 
   Size:       1800     281    5439

  Fragments arranged by size: 

                5439    1800     281
 
AhdI GACnn_n'nnGTC

Cuts at:    897     897 
   Size:       7520
 
AleI CACnn'nnGTG

Cuts at:   1702    1822    1942    2062    2182    2302    2422    2542    3008 
   Size:        120     120     120     120     120     120     120     466

Cuts at:   3008    3487    4210    1702 
   Size:        479     723    5012

  Fragments arranged by size: 

                5012     723     479     466     120     120     120     120
                 120     120     120
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3457    3457 
   Size:       7520
 
AlwNI CAG_nnn'CTG

Cuts at:    141     337     433     921    2707    2764    2854    3326    3699 
   Size:        196      96     488    1786      57      90     472     373

Cuts at:   3699    4825    6263     141 
   Size:       1126    1438    1398

  Fragments arranged by size: 

                1786    1438    1398    1126     488     472     373     196
                  96      90      57
 
ApaI G_GGCC'C

Cuts at:    407    3508    3830    3915     407 
   Size:       3101     322      85    4012

  Fragments arranged by size: 

                4012    3101     322      85
 
ApaLI G'TGCA_C

Cuts at:   4255    6358    4255 
   Size:       2103    5417
 
ApoI r'AATT_y

Cuts at:   4524    6873    7129    4524 
   Size:       2349     256    4915

  Fragments arranged by size: 

                4915    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7520
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7520
 
AvaI C'yCGr_G

Cuts at:    286     477    3865    3908    3921    4331    4540     286 
   Size:        191    3388      43      13     410     209    3266

  Fragments arranged by size: 

                3388    3266     410     209     191      43      13
 
BamHI G'GATC_C

Cuts at:    849    4545     849 
   Size:       3696    3824
 
BanI G'GyrC_C

Cuts at:   3433    3526    4284    4536    4950    5198    5233    3433 
   Size:         93     758     252     414     248      35    5720

  Fragments arranged by size: 

                5720     758     414     252     248      93      35
 
BanII G_rGCy'C

Cuts at:    179     407     484     647    1055    3193    3239    3508    3563 
   Size:        228      77     163     408    2138      46     269      55

Cuts at:   3563    3620    3830    3838    3857    3915    3922    4195    4298 
   Size:         57     210       8      19      58       7     273     103

Cuts at:   4298    4464    4534    5564     179 
   Size:        166      70    1030    2135

  Fragments arranged by size: 

                2138    2135    1030     408     273     269     228     210
                 166     163     103      77      70      58      57      55
                  46      19       8       7
 
BbeI G_GCGC'C

Cuts at:   4954    5202    4954 
   Size:        248    7272
 
BbsI GAAGACnn'nnnn_

Cuts at:    122    1099    3284    3602    3614    3905    4176     122 
   Size:        977    2185     318      12     291     271    3466

  Fragments arranged by size: 

                3466    2185     977     318     291     271      12
 
BbvCI CC'TCA_GC

Cuts at:    535    1403    3249    3745    4299     535 
   Size:        868    1846     496     554    3756

  Fragments arranged by size: 

                3756    1846     868     554     496
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     943     964     977     998      90 
   Size:         34     819      21      13      21    6612

  Fragments arranged by size: 

                6612     819      34      21      21      13
 
BciVI GTATCCnnnnn_n'

Cuts at:   5418    6474    7520    5418 
   Size:       1056    1046    5418

  Fragments arranged by size: 

                5418    1056    1046
 
BclI T'GATC_A

Cuts at:   5040    5040 
   Size:       7520
 
BglI GCCn_nnn'nGGC

Cuts at:    701    1165    2719    3218    3265    3842    4084    4459     701 
   Size:        464    1554     499      47     577     242     375    3762

  Fragments arranged by size: 

                3762    1554     577     499     464     375     242      47
            
 
BglII A'GATC_T

Cuts at:   1430    1662    1902    2022    2142    2262    2382    2502    3791 
   Size:        232     240     120     120     120     120     120    1289

Cuts at:   3791    5035    7072    1430 
   Size:       1244    2037    1878

  Fragments arranged by size: 

                2037    1878    1289    1244     240     232     120     120
                 120     120     120
 
BlpI GC'TnA_GC

Cuts at:    434    2723    3020    4632     434 
   Size:       2289     297    1612    3322

  Fragments arranged by size: 

                3322    2289    1612     297
 
Bme1580I G_kGCm'C

Cuts at:    266     407    2694    3508    3830    3915    4259    4289    5145 
   Size:        141    2287     814     322      85     344      30     856

Cuts at:   5145    5238    6362     266 
   Size:         93    1124    1424

  Fragments arranged by size: 

                2287    1424    1124     856     814     344     322     141
                  93      85      30
 
BmgBI CAC'GTC

Cuts at:    203     899    3350     203 
   Size:        696    2451    4373

  Fragments arranged by size: 

                4373    2451     696
 
BmrI ACTGGGnnnn_n'

Cuts at:   3394    3416    3716    4732    4813    5148    7292    3394 
   Size:         22     300    1016      81     335    2144    3622

  Fragments arranged by size: 

                3622    2144    1016     335     300      81      22
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2946    3739    3918    5887    2946 
   Size:        793     179    1969    4579

  Fragments arranged by size: 

                4579    1969     793     179
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    193    1060    1294    3070    3577    3913    4363     193 
   Size:        867     234    1776     507     336     450    3350

  Fragments arranged by size: 

                3350    1776     867     507     450     336     234
 
Bpu10I CC'TnA_GC

Cuts at:    244     535    1136    1403    2921    3187    3249    3745    4299 
   Size:        291     601     267    1518     266      62     496     554

Cuts at:   4299     244 
   Size:       3465

  Fragments arranged by size: 

                3465    1518     601     554     496     291     267     266
                  62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3833    4696    5575    6289    6587    3833 
   Size:        863     879     714     298    4766

  Fragments arranged by size: 

                4766     879     863     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3037    4030    7224    3037 
   Size:        993    3194    3333

  Fragments arranged by size: 

                3333    3194     993
 
BsaAI yAC'GTr

Cuts at:   4873    4873 
   Size:       7520
 
BsaBI GATnn'nnATC

Cuts at:      6    4135    5034    7071       6 
   Size:       4129     899    2037     455

  Fragments arranged by size: 

                4129    2037     899     455
 
BsaHI Gr'CG_yC

Cuts at:    416    4951    5199    5901    7397     416 
   Size:       4535     248     702    1496     539

  Fragments arranged by size: 

                4535    1496     702     539     248
 
BsaWI w'CCGG_w

Cuts at:   4709    5230    5908    6319    6466    6740    4709 
   Size:        521     678     411     147     274    5489

  Fragments arranged by size: 

                5489     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    138     168     667     697     994    1024     138 
   Size:         30     499      30     297      30    6634

  Fragments arranged by size: 

                6634     499     297      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    103     146     211     675     850     901     981    1114    1117 
   Size:         43      65     464     175      51      80     133       3

Cuts at:   1117    1163    1502    2782    3628    3733    3806    3977    4048 
   Size:         46     339    1280     846     105      73     171      71

Cuts at:   4048    4378    4381    4384     103 
   Size:        330       3       3    3239

  Fragments arranged by size: 

                3239    1280     846     464     339     330     175     171
                 133     105      80      73      71      65      51      46
                  43       3       3       3
 
BseYI C'CCAG_C

Cuts at:    509     578     627     874    1345    1709    1829    1949    2069 
   Size:         69      49     247     471     364     120     120     120

Cuts at:   2069    2189    2309    2429    2549    2854    2938    3192    3365 
   Size:        120     120     120     120     305      84     254     173

Cuts at:   3365    3478    3535    3564    3837    4017    4098    4194    4247 
   Size:        113      57      29     273     180      81      96      53

Cuts at:   4247    4945    5703    6368    6776     509 
   Size:        698     758     665     408    1253

  Fragments arranged by size: 

                1253     758     698     665     471     408     364     305
                 273     254     247     180     173     120     120     120
                 120     120     120     120     113      96      84      81
                  69      57      53      49      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    607    3338    4018     607 
   Size:       2731     680    4109

  Fragments arranged by size: 

                4109    2731     680
 
BsiEI CG_ry'CG

Cuts at:    104     497    5062    5108    6338     104 
   Size:        393    4565      46    1230    1286

  Fragments arranged by size: 

                4565    1286    1230     393      46
 
BsiHKAI G_wGCw'C

Cuts at:   4259    4534    5312    5502    6362    4259 
   Size:        275     778     190     860    5417

  Fragments arranged by size: 

                5417     860     778     275     190
 
BsmI GAATG_Cn'

Cuts at:   2781    3930    2781 
   Size:       1149    6371
 
BsmBI CGTCTCn'nnnn_

Cuts at:    193     193 
   Size:       7520
 
Bsp1286I G_dGCh'C

Cuts at:    179     266     407     484     647    1055    2694    3193    3239 
   Size:         87     141      77     163     408    1639     499      46

Cuts at:   3239    3508    3563    3620    3830    3838    3857    3915    3922 
   Size:        269      55      57     210       8      19      58       7

Cuts at:   3922    4195    4259    4289    4298    4464    4534    5145    5238 
   Size:        273      64      30       9     166      70     611      93

Cuts at:   5238    5312    5502    5564    6362     179 
   Size:         74     190      62     798    1337

  Fragments arranged by size: 

                1639    1337     798     611     499     408     273     269
                 210     190     166     163     141      93      87      77
                  74      70      64      62      58      57      55      46
                  30      19       9       8       7
 
BspHI T'CATG_A

Cuts at:   5952    5952 
   Size:       7520
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1284    4570    4784    5086    5467    1284 
   Size:       3286     214     302     381    3337

  Fragments arranged by size: 

                3337    3286     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1109    5812    5866    7350    7515      50 
   Size:       1059    4703      54    1484     165      55

  Fragments arranged by size: 

                4703    1484    1059     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1403    4094    5432    1403 
   Size:       2691    1338    3491

  Fragments arranged by size: 

                3491    2691    1338
 
BsrFI r'CCGG_y

Cuts at:   2925    5518    5699    2925 
   Size:       2593     181    4746

  Fragments arranged by size: 

                4746    2593     181
 
BsrGI T'GTAC_A

Cuts at:   1040    1040 
   Size:       7520
 
BssSI C'ACGA_G

Cuts at:   5791    6499    5791 
   Size:        708    6812
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2689    3795    4897    2689 
   Size:       1106    1102    5312

  Fragments arranged by size: 

                5312    1106    1102
 
BstBI TT'CG_AA

Cuts at:   5881    5881 
   Size:       7520
 
BstXI CCAn_nnnn'nTGG

Cuts at:    259    3780     259 
   Size:       3521    3999
 
BstYI r'GATC_y

Cuts at:      1     849    1430    1662    1902    2022    2142    2262    2382 
   Size:        848     581     232     240     120     120     120     120

Cuts at:   2382    2502    3791    4545    5012    5035    5370    5616    5922 
   Size:        120    1289     754     467      23     335     246     306

Cuts at:   5922    5934    6020    7072       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1289    1052     848     754     581     467     449     335
                 306     246     240     232     120     120     120     120
                 120      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    119     573    2631    2745    2765    3164     119 
   Size:        454    2058     114      20     399    4475

  Fragments arranged by size: 

                4475    2058     454     399     114      20
 
BtgI C'CryG_G

Cuts at:    146     408     602    1160    1391    2717    3488    3764    3942 
   Size:        262     194     558     231    1326     771     276     178

Cuts at:   3942    4511     146 
   Size:        569    3155

  Fragments arranged by size: 

                3155    1326     771     569     558     276     262     231
                 194     178
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4026    4152    5470    5651    5814    4026 
   Size:        126    1318     181     163    5732

  Fragments arranged by size: 

                5732    1318     181     163     126
 
BtsI GCAGTG_nn'

Cuts at:    803    1265    1451    3402    4006    4035    4071    4763     803 
   Size:        462     186    1951     604      29      36     692    3560

  Fragments arranged by size: 

                3560    1951     692     604     462     186      36      29
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1667    1702    1787    1822    1907    1942    2027    2062    2147 
   Size:         35      85      35      85      35      85      35      85

Cuts at:   2147    2182    2267    2302    2387    2422    2507    2542    1667 
   Size:         35      85      35      85      35      85      35    6645

  Fragments arranged by size: 

                6645      85      85      85      85      85      85      85
                  35      35      35      35      35      35      35      35
            
 
DraI TTT'AAA

Cuts at:   4519    4519 
   Size:       7520
 
DraIII CAC_nnn'GTG

Cuts at:   1499    1499 
   Size:       7520
 
DrdI GACnn_nn'nnGTC

Cuts at:   5226    6570    5226 
   Size:       1344    6176
 
EaeI y'GGCC_r

Cuts at:    164     494     728    2654    2711    3257    5105    5279    5670 
   Size:        330     234    1926      57     546    1848     174     391

Cuts at:   5670    5697    7042     164 
   Size:         27    1345     642

  Fragments arranged by size: 

                1926    1848    1345     642     546     391     330     234
                 174      57      27
 
EagI C'GGCC_G

Cuts at:    494    5105     494 
   Size:       4611    2909
 
EarI CTCTTCn'nnn_

Cuts at:    522     827    1232    1715    1835    1955    2075    2195    2315 
   Size:        305     405     483     120     120     120     120     120

Cuts at:   2315    2435    2555    4349    4355    4370    5543    5753     522 
   Size:        120     120    1794       6      15    1173     210    2289

  Fragments arranged by size: 

                2289    1794    1173     483     405     305     210     120
                 120     120     120     120     120     120      15       6
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    862    5728    6470    6616    7330     862 
   Size:       4866     742     146     714    1052

  Fragments arranged by size: 

                4866    1052     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   4532    4532 
   Size:       7520
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    193     575    1060    1294    1739    1859    1979    2099    2219 
   Size:        382     485     234     445     120     120     120     120

Cuts at:   2219    2339    2459    2579    3070    3100    3278    3577    3913 
   Size:        120     120     120     491      30     178     299     336

Cuts at:   3913    4363    5345    5777    6130    6792     193 
   Size:        450     982     432     353     662     921

  Fragments arranged by size: 

                 982     921     662     491     485     450     445     432
                 382     353     336     299     234     178     120     120
                 120     120     120     120     120      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:    118    3466    3580     118 
   Size:       3348     114    4058

  Fragments arranged by size: 

                4058    3348     114
 
EcoO109I rG'GnC_Cy

Cuts at:    123     210     404     582     635     867    1193    1242    1605 
   Size:         87     194     178      53     232     326      49     363

Cuts at:   1605    1725    1845    1965    2085    2205    2325    2445    2565 
   Size:        120     120     120     120     120     120     120     120

Cuts at:   2565    2623    2749    2907    3135    3497    3505    3585    3827 
   Size:         58     126     158     228     362       8      80     242

Cuts at:   3827    3912    4014    4239    4328    4659     123 
   Size:         85     102     225      89     331    2984

  Fragments arranged by size: 

                2984     363     362     331     326     242     232     228
                 225     194     178     158     126     120     120     120
                 120     120     120     120     120     102      89      87
                  85      80      58      53      49       8
 
EcoRI G'AATT_C

Cuts at:   4524    4524 
   Size:       7520
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1261    4119    5539    1261 
   Size:       2858    1420    3242

  Fragments arranged by size: 

                3242    2858    1420
 
FspI TGC'GCA

Cuts at:   5301    5301 
   Size:       7520
 
HaeII r_GCGC'y

Cuts at:    244    4954    5202    6432    7203     244 
   Size:       4710     248    1230     771     561

  Fragments arranged by size: 

                4710    1230     771     561     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    112     144     556     588     634     666     667     699     883 
   Size:         32     412      32      46      32       1      32     184

Cuts at:    883     915     964     988     994     996    1020    1026    1534 
   Size:         32      49      24       6       2      24       6     508

Cuts at:   1534    1566    1626    1654    1658    1686    1746    1778    1866 
   Size:         32      60      28       4      28      60      32      88

Cuts at:   1866    1894    1898    1926    1986    2014    2018    2046    2106 
   Size:         28       4      28      60      28       4      28      60

Cuts at:   2106    2134    2138    2166    2226    2254    2258    2286    2346 
   Size:         28       4      28      60      28       4      28      60

Cuts at:   2346    2374    2378    2406    2466    2494    2498    2526    2586 
   Size:         28       4      28      60      28       4      28      60

Cuts at:   2586    2618    4228    4260    5362    5392    5394    5424     112 
   Size:         32    1610      32    1102      30       2      30    2208

  Fragments arranged by size: 

                2208    1610    1102     508     412     184      88      60
                  60      60      60      60      60      60      60      49
                  46      32      32      32      32      32      32      32
                  32      32      30      30      28      28      28      28
                  28      28      28      28      28      28      28      28
                  28      28      24      24       6       6       4       4
                   4       4       4       4       4       2       2       1
            
 
HincII GTy'rAC

Cuts at:   4491    4559    4491 
   Size:         68    7452
 
KasI G'GCGC_C

Cuts at:   4950    5198    4950 
   Size:        248    7272
 
KpnI G_GTAC'C

Cuts at:   3530    4540    3530 
   Size:       1010    6510
 
MluI A'CGCG_T

Cuts at:   6953    6953 
   Size:       7520
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1492    2847    6279    6463    1492 
   Size:       1355    3432     184    2549

  Fragments arranged by size: 

                3432    2549    1355     184
 
MscI TGG'CCA

Cuts at:    730    2656    2713    3259    5281     730 
   Size:       1926      57     546    2022    2969

  Fragments arranged by size: 

                2969    2022    1926     546      57
 
MslI CAynn'nnrTG

Cuts at:   1396    1702    1822    1942    2062    2182    2302    2422    2542 
   Size:        306     120     120     120     120     120     120     120

Cuts at:   2542    3008    3487    4210    5509    5636    1396 
   Size:        466     479     723    1299     127    3280

  Fragments arranged by size: 

                3280    1299     723     479     466     306     127     120
                 120     120     120     120     120     120
 
MspA1I CmG'CkG

Cuts at:    509    1046    1372    2845    3253    3670    3841    4270    4631 
   Size:        537     326    1473     408     417     171     429     361

Cuts at:   4631    4945    5305    6089    6334     509 
   Size:        314     360     784     245    1695

  Fragments arranged by size: 

                1695    1473     784     537     429     417     408     361
                 360     326     314     245     171
 
NaeI GCC'GGC

Cuts at:   2927    5701    2927 
   Size:       2774    4746
 
NarI GG'CG_CC

Cuts at:   4951    5199    4951 
   Size:        248    7272
 
NcoI C'CATG_G

Cuts at:    602    1391    2717    3764    4511     602 
   Size:        789    1326    1047     747    3611

  Fragments arranged by size: 

                3611    1326    1047     789     747
 
NgoMIV G'CCGG_C

Cuts at:   2925    5699    2925 
   Size:       2774    4746
 
NspI r_CATG'y

Cuts at:   2987    4573    5073    6676    2987 
   Size:       1586     500    1603    3831

  Fragments arranged by size: 

                3831    1603    1586     500
 
PasI CC'CwG_GG

Cuts at:   2695    3501    3915    2695 
   Size:        806     414    6300

  Fragments arranged by size: 

                6300     806     414
 
PciI A'CATG_T

Cuts at:   6672    6672 
   Size:       7520
 
PflMI CCAn_nnn'nTGG

Cuts at:   1345    1345 
   Size:       7520
 
PfoI T'CCnGG_A

Cuts at:    968    1076    2795     968 
   Size:        108    1719    5693

  Fragments arranged by size: 

                5693    1719     108
 
PmeI GTTT'AAAC

Cuts at:   4519    4519 
   Size:       7520
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5966    5998    5966 
   Size:         32    7488
 
PpuMI rG'GwC_Cy

Cuts at:    635     867    1605    3135    3585    4239     635 
   Size:        232     738    1530     450     654    3916

  Fragments arranged by size: 

                3916    1530     738     654     450     232
 
PshAI GACnn'nnGTC

Cuts at:   3108    3108 
   Size:       7520
 
PspOMI G'GGCC_C

Cuts at:    403    3504    3826    3911     403 
   Size:       3101     322      85    4012

  Fragments arranged by size: 

                4012    3101     322      85
 
PstI C_TGCA'G

Cuts at:    675     917     987    1133    1295    1458    1575    1695    1815 
   Size:        242      70     146     162     163     117     120     120

Cuts at:   1815    1935    2055    2175    2295    2415    2535    2808    2850 
   Size:        120     120     120     120     120     120     273      42

Cuts at:   2850    3449    4567    5252     675 
   Size:        599    1118     685    2943

  Fragments arranged by size: 

                2943    1118     685     599     273     242     163     162
                 146     120     120     120     120     120     120     120
                 120     117      70      42
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7520
 
PvuII CAG'CTG

Cuts at:    509    2845    3253    3841    4270    4945    5305     509 
   Size:       2336     408     588     429     675     360    2724

  Fragments arranged by size: 

                2724    2336     675     588     429     408     360
 
RsrII CG'GwC_CG

Cuts at:   5715    5715 
   Size:       7520
 
SacI G_AGCT'C

Cuts at:   4534    4534 
   Size:       7520
 
SalI G'TCGA_C

Cuts at:   4557    4557 
   Size:       7520
 
SanDI GG'GwC_CC

Cuts at:    635     635 
   Size:       7520
 
SapI GCTCTTCn'nnn_

Cuts at:   5543    5753    5543 
   Size:        210    7310
 
SbfI CC_TGCA'GG

Cuts at:   4567    4567 
   Size:       7520
 
ScaI AGT'ACT

Cuts at:   7037    7037 
   Size:       7520
 
SexAI A'CCwGG_T

Cuts at:   2816    2816 
   Size:       7520
 
SfcI C'TryA_G

Cuts at:     33     671     913     983    1129    1291    1454    1565    1571 
   Size:        638     242      70     146     162     163     111       6

Cuts at:   1571    1691    1811    1931    2051    2171    2291    2411    2531 
   Size:        120     120     120     120     120     120     120     120

Cuts at:   2531    2789    2804    2846    3445    4563    5248    6216    6407 
   Size:        258      15      42     599    1118     685     968     191

Cuts at:   6407      33 
   Size:       1146

  Fragments arranged by size: 

                1146    1118     968     685     638     599     258     242
                 191     163     162     146     120     120     120     120
                 120     120     120     120     111      70      42      15
                   6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    701    3265     701 
   Size:       2564    4956
 
SfoI GGC'GCC

Cuts at:   4952    5200    4952 
   Size:        248    7272
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7520
 
SmaI CCC'GGG

Cuts at:   4333    4542    4333 
   Size:        209    7311
 
SmlI C'TyrA_G

Cuts at:   3848    4675    5590    6304    6566    3848 
   Size:        827     915     714     262    4802

  Fragments arranged by size: 

                4802     915     827     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       7520
 
SphI G_CATG'C

Cuts at:   4573    5073    4573 
   Size:        500    7020
 
StuI AGG'CCT

Cuts at:   1307    2743    2970    3077    3212    1307 
   Size:       1436     227     107     135    5615

  Fragments arranged by size: 

                5615    1436     227     135     107
 
StyI C'CwwG_G

Cuts at:    602    1121    1391    2717    2810    2863    3429    3467    3752 
   Size:        519     270    1326      93      53     566      38     285

Cuts at:   3752    3764    4304    4448    4511    4654    5008     602 
   Size:         12     540     144      63     143     354    3114

  Fragments arranged by size: 

                3114    1326     566     540     519     354     285     270
                 144     143      93      63      53      38      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    237     641    4338    5103    5236    5695    5904     237 
   Size:        404    3697     765     133     459     209    1853

  Fragments arranged by size: 

                3697    1853     765     459     404     209     133
 
TatI w'GTAC_w

Cuts at:   1040    3381    7035    1040 
   Size:       2341    3654    1525

  Fragments arranged by size: 

                3654    2341    1525
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    138     170    1485    1517    2619    2651    7037    7069     138 
   Size:         32    1315      32    1102      32    4386      32     589

  Fragments arranged by size: 

                4386    1315    1102     589      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1084    1149    2818    4486    5317    1084 
   Size:         65    1669    1668     831    3287

  Fragments arranged by size: 

                3287    1669    1668     831      65
 
XbaI T'CTAG_A

Cuts at:   4551    4551 
   Size:       7520
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2898    3486    3696    3838    5015    2898 
   Size:        588     210     142    1177    5403

  Fragments arranged by size: 

                5403    1177     588     210     142
 
XmaI C'CCGG_G

Cuts at:   4331    4540    4331 
   Size:        209    7311
 
XmnI GAAnn'nnTTC

Cuts at:   1463    1463 
   Size:       7520
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AloI     AscI    AvrII     BaeI
    BfrBI     BmtI    BsiWI    BspEI   BssHII   BstEII  BstZ17I     ClaI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmlI     PsiI    PspXI     PsrI
    SacII    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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