Restriction map for pF1KA0339
 (Linear) (Six-base) MAPSORT of: KIAA0339.seq  Check: 2441  from: 1  to: 5121



KIAA0339.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2255    3417    5121 
   Size:       2255    1162    1704

  Fragments arranged by size: 

                2255    1704    1162
 
AatII G_ACGT'C

Cuts at:      0    2808    5121 
   Size:       2808    2313
 
Acc65I G'GTAC_C

Cuts at:      0    4642    5121 
   Size:       4642     479
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     298     349     935    1507    2584    3206    3966 
   Size:        123     175      51     586     572    1077     622     760

Cuts at:   3966    5121 
   Size:       1155

  Fragments arranged by size: 

                1155    1077     760     622     586     572     175     123
                  51
 
AfeI AGC'GCT

Cuts at:      0    1210    1498    4837    5121 
   Size:       1210     288    3339     284

  Fragments arranged by size: 

                3339    1210     288     284
 
AflIII A'CryG_T

Cuts at:      0     818    4535    5121 
   Size:        818    3717     586

  Fragments arranged by size: 

                3717     818     586
 
AleI CACnn'nnGTG

Cuts at:      0     140     697    5121 
   Size:        140     557    4424

  Fragments arranged by size: 

                4424     557     140
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1495    3920    5121 
   Size:       1495    2425    1201

  Fragments arranged by size: 

                2425    1495    1201
 
AlwNI CAG_nnn'CTG

Cuts at:      0     631    1000    1318    3271    4059    5121 
   Size:        631     369     318    1953     788    1062

  Fragments arranged by size: 

                1953    1062     788     631     369     318
 
ApaI G_GGCC'C

Cuts at:      0    1346    1613    2661    3419    4457    5121 
   Size:       1346     267    1048     758    1038     664

  Fragments arranged by size: 

                1346    1048    1038     758     664     267
 
ApoI r'AATT_y

Cuts at:      0    2705    5121 
   Size:       2705    2416
 
AvaI C'yCGr_G

Cuts at:      0      36     434     618     824    1243    1685    2292    3599 
   Size:         36     398     184     206     419     442     607    1307

Cuts at:   3599    3664    3677    4015    4296    4457    5121 
   Size:         65      13     338     281     161     664

  Fragments arranged by size: 

                1307     664     607     442     419     398     338     281
                 206     184     161      65      36      13
 
AvrII C'CTAG_G

Cuts at:      0    3865    5121 
   Size:       3865    1256
 
BamHI G'GATC_C

Cuts at:      0      72    4727    5121 
   Size:         72    4655     394

  Fragments arranged by size: 

                4655     394      72
 
BanI G'GyrC_C

Cuts at:      0     439     688     718    1587    1653    2365    3279    3525 
   Size:        439     249      30     869      66     712     914     246

Cuts at:   3525    4159    4196    4642    5121 
   Size:        634      37     446     479

  Fragments arranged by size: 

                 914     869     712     634     479     446     439     249
                 246      66      37      30
 
BanII G_rGCy'C

Cuts at:      0    1185    1250    1346    1613    1994    2013    2273    2661 
   Size:       1185      65      96     267     381      19     260     388

Cuts at:   2661    3419    3542    4046    4457    5113    5121 
   Size:        758     123     504     411     656       8

  Fragments arranged by size: 

                1185     758     656     504     411     388     381     267
                 260     123      96      65      19       8
 
BbeI G_GCGC'C

Cuts at:      0     443    4163    4200    5121 
   Size:        443    3720      37     921

  Fragments arranged by size: 

                3720     921     443      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     179    2560    2771    3032    3830    5072    5121 
   Size:        179    2381     211     261     798    1242      49

  Fragments arranged by size: 

                2381    1242     798     261     211     179      49
 
BbvCI CC'TCA_GC

Cuts at:      0    1802    2106    2753    3225    4031    5121 
   Size:       1802     304     647     472     806    1090

  Fragments arranged by size: 

                1802    1090     806     647     472     304
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    4575    4609    5121 
   Size:       4575      34     512

  Fragments arranged by size: 

                4575     512      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2976    5121 
   Size:       2976    2145
 
BglI GCCn_nnn'nGGC

Cuts at:      0     973    1254    1291    3775    5121 
   Size:        973     281      37    2484    1346

  Fragments arranged by size: 

                2484    1346     973     281      37
 
BglII A'GATC_T

Cuts at:      0    1751    5121 
   Size:       1751    3370
 
BlpI GC'TnA_GC

Cuts at:      0    1205    3734    3877    4476    4630    5121 
   Size:       1205    2529     143     599     154     491

  Fragments arranged by size: 

                2529    1205     599     491     154     143
 
Bme1580I G_kGCm'C

Cuts at:      0     581     706    1346    1442    1613    2614    2661    3260 
   Size:        581     125     640      96     171    1001      47     599

Cuts at:   3260    3419    3965    4457    4543    5121 
   Size:        159     546     492      86     578

  Fragments arranged by size: 

                1001     640     599     581     578     546     492     171
                 159     125      96      86      47
 
BmgBI CAC'GTC

Cuts at:      0     821    4536    5121 
   Size:        821    3715     585

  Fragments arranged by size: 

                3715     821     585
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     223     593    2604    3247    3304    3374    4401    4537 
   Size:        223     370    2011     643      57      70    1027     136

Cuts at:   4537    5121 
   Size:        584

  Fragments arranged by size: 

                2011    1027     643     584     370     223     136      70
                  57
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1515    2934    3198    3468    3730    4143    5121 
   Size:       1515    1419     264     270     262     413     978

  Fragments arranged by size: 

                1515    1419     978     413     270     264     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     732     846    1199    1295    1757    2813    3326    3530 
   Size:        732     114     353      96     462    1056     513     204

Cuts at:   3530    3719    3913    4027    4582    5121 
   Size:        189     194     114     555     539

  Fragments arranged by size: 

                1056     732     555     539     513     462     353     204
                 194     189     114     114      96
 
Bpu10I CC'TnA_GC

Cuts at:      0    1802    2106    2753    3225    4031    5121 
   Size:       1802     304     647     472     806    1090

  Fragments arranged by size: 

                1802    1090     806     647     472     304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4672    5121 
   Size:       4672     449
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     313    1414    2417    5121 
   Size:        313    1101    1003    2704

  Fragments arranged by size: 

                2704    1101    1003     313
 
BsaAI yAC'GTr

Cuts at:      0    4337    4791    4842    5121 
   Size:       4337     454      51     279

  Fragments arranged by size: 

                4337     454     279      51
 
BsaBI GATnn'nnATC

Cuts at:      0    2963    4273    4780    4999    5121 
   Size:       2963    1310     507     219     122

  Fragments arranged by size: 

                2963    1310     507     219     122
 
BsaHI Gr'CG_yC

Cuts at:      0     440    1795    1855    2805    3337    4160    4197    5121 
   Size:        440    1355      60     950     532     823      37     924

  Fragments arranged by size: 

                1355     950     924     823     532     440      60      37
            
 
BsaWI w'CCGG_w

Cuts at:      0    4166    4694    5121 
   Size:       4166     528     427

  Fragments arranged by size: 

                4166     528     427
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     457     487    1608    1638    4108    4138    5121 
   Size:        457      30    1121      30    2470      30     983

  Fragments arranged by size: 

                2470    1121     983     457      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1014    1032    1050    1352    1546    1549    1829    1832 
   Size:       1014      18      18     302     194       3     280       3

Cuts at:   1832    1888    1937    2271    2274    2314    2506    2554    2907 
   Size:         56      49     334       3      40     192      48     353

Cuts at:   2907    2911    2947    3111    3120    3123    3126    3129    3144 
   Size:          4      36     164       9       3       3       3      15

Cuts at:   3144    3171    3217    3382    3682    3695    3742    4054    4090 
   Size:         27      46     165     300      13      47     312      36

Cuts at:   4090    4102    4129    4132    4135    5047    5121 
   Size:         12      27       3       3     912      74

  Fragments arranged by size: 

                1014     912     353     334     312     302     300     280
                 194     192     165     164      74      56      49      48
                  47      46      40      36      36      27      27      18
                  18      15      13      12       9       4       3       3
                   3       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     500     681    1098    1249    1345    1383    1410    1448 
   Size:        500     181     417     151      96      38      27      38

Cuts at:   1448    1632    3412    3579    3990    5121 
   Size:        184    1780     167     411    1131

  Fragments arranged by size: 

                1780    1131     500     417     411     184     181     167
                 151      96      38      38      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     644    1599    4864    4929    5121 
   Size:        644     955    3265      65     192

  Fragments arranged by size: 

                3265     955     644     192      65
 
BsiEI CG_ry'CG

Cuts at:      0     641    1767    5121 
   Size:        641    1126    3354

  Fragments arranged by size: 

                3354    1126     641
 
BsiHKAI G_wGCw'C

Cuts at:      0     422    1994    2384    3899    4466    5121 
   Size:        422    1572     390    1515     567     655

  Fragments arranged by size: 

                1572    1515     655     567     422     390
 
BsiWI C'GTAC_G

Cuts at:      0     388    4337    5121 
   Size:        388    3949     784

  Fragments arranged by size: 

                3949     784     388
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     628     827    1733    3344    5121 
   Size:        628     199     906    1611    1777

  Fragments arranged by size: 

                1777    1611     906     628     199
 
Bsp1286I G_dGCh'C

Cuts at:      0     422     581     706    1185    1250    1346    1442    1613 
   Size:        422     159     125     479      65      96      96     171

Cuts at:   1613    1994    2013    2273    2384    2614    2661    3260    3419 
   Size:        381      19     260     111     230      47     599     159

Cuts at:   3419    3542    3899    3965    4046    4457    4466    4543    5113 
   Size:        123     357      66      81     411       9      77     570

Cuts at:   5113    5121 
   Size:          8

  Fragments arranged by size: 

                 599     570     479     422     411     381     357     260
                 230     171     159     159     125     123     111      96
                  96      81      77      66      65      47      19       9
                   8
 
BspEI T'CCGG_A

Cuts at:      0    4166    4694    5121 
   Size:       4166     528     427

  Fragments arranged by size: 

                4166     528     427
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1244    1896    2255    3417    3635    4334    4676    5121 
   Size:       1244     652     359    1162     218     699     342     445

  Fragments arranged by size: 

                1244    1162     699     652     445     359     342     218
            
 
BsrBI CCG'CTC

Cuts at:      0     656    1400    1667    2606    3207    3612    4184    4344 
   Size:        656     744     267     939     601     405     572     160

Cuts at:   4344    5121 
   Size:        777

  Fragments arranged by size: 

                 939     777     744     656     601     572     405     267
                 160
 
BsrDI GCAATG_nn'

Cuts at:      0    4760    5121 
   Size:       4760     361
 
BsrFI r'CCGG_y

Cuts at:      0    1253    1274    4607    5121 
   Size:       1253      21    3333     514

  Fragments arranged by size: 

                3333    1253     514      21
 
BssSI C'ACGA_G

Cuts at:      0    2587    4732    5121 
   Size:       2587    2145     389

  Fragments arranged by size: 

                2587    2145     389
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     725    1000    3352    4059    5121 
   Size:        725     275    2352     707    1062

  Fragments arranged by size: 

                2352    1062     725     707     275
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     699    2259    3522    5025    5121 
   Size:        699    1560    1263    1503      96

  Fragments arranged by size: 

                1560    1503    1263     699      96
 
BstYI r'GATC_y

Cuts at:      0      72     371    1751    4727    5075    5121 
   Size:         72     299    1380    2976     348      46

  Fragments arranged by size: 

                2976    1380     348     299      72      46
 
BtgI C'CryG_G

Cuts at:      0     971    2369    3362    4751    5121 
   Size:        971    1398     993    1389     370

  Fragments arranged by size: 

                1398    1389     993     971     370
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     963    4163    5121 
   Size:        963    3200     958

  Fragments arranged by size: 

                3200     963     958
 
BtsI GCAGTG_nn'

Cuts at:      0     692     989     998    4260    4938    5121 
   Size:        692     297       9    3262     678     183

  Fragments arranged by size: 

                3262     692     678     297     183       9
 
ClaI AT'CG_AT

Cuts at:      0    4898    5121 
   Size:       4898     223
 
DraIII CAC_nnn'GTG

Cuts at:      0    4255    4910    5121 
   Size:       4255     655     211

  Fragments arranged by size: 

                4255     655     211
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3006    3760    4777    5121 
   Size:       3006     754    1017     344

  Fragments arranged by size: 

                3006    1017     754     344
 
EaeI y'GGCC_r

Cuts at:      0     638     780     974    2519    3208    3767    3911    4716 
   Size:        638     142     194    1545     689     559     144     805

Cuts at:   4716    4817    4922    5121 
   Size:        101     105     199

  Fragments arranged by size: 

                1545     805     689     638     559     199     194     144
                 142     105     101
 
EagI C'GGCC_G

Cuts at:      0     638    5121 
   Size:        638    4483
 
EarI CTCTTCn'nnn_

Cuts at:      0     356     427     663    1023    1059    1535    1737    2401 
   Size:        356      71     236     360      36     476     202     664

Cuts at:   2401    2831    3117    3168    3188    3194    3459    3719    3985 
   Size:        430     286      51      20       6     265     260     266

Cuts at:   3985    4079    4094    4106    5121 
   Size:         94      15      12    1015

  Fragments arranged by size: 

                1015     664     476     430     360     356     286     266
                 265     260     236     202      94      71      51      36
                  20      15      12       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      66    1002    1802    4051    4199    4638    5121 
   Size:         66     936     800    2249     148     439     483

  Fragments arranged by size: 

                2249     936     800     483     439     148      66
 
EcoICRI GAG'CTC

Cuts at:      0    1992    5121 
   Size:       1992    3129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     298     349     732     846     935    1199    1295 
   Size:        123     175      51     383     114      89     264      96

Cuts at:   1295    1507    1757    2584    2813    3206    3326    3530    3719 
   Size:        212     250     827     229     393     120     204     189

Cuts at:   3719    3913    3966    4027    4582    5121 
   Size:        194      53      61     555     539

  Fragments arranged by size: 

                 827     555     539     393     383     264     250     229
                 212     204     194     189     175     123     120     114
                  96      89      61      53      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1322    3718    5121 
   Size:       1322    2396    1403

  Fragments arranged by size: 

                2396    1403    1322
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     594    1342    1610    2126    2657    2658    3358 
   Size:         33     561     748     268     516     531       1     700

Cuts at:   3358    3416    3869    4002    4011    4454    5121 
   Size:         58     453     133       9     443     667

  Fragments arranged by size: 

                 748     700     667     561     531     516     453     443
                 268     133      58      33       9       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1527    5121 
   Size:       1527    3594
 
FspI TGC'GCA

Cuts at:      0      97    5121 
   Size:         97    5024
 
HaeII r_GCGC'y

Cuts at:      0     443    1134    1212    1500    3864    4163    4200    4608 
   Size:        443     691      78     288    2364     299      37     408

Cuts at:   4608    4637    4839    5121 
   Size:         29     202     282

  Fragments arranged by size: 

                2364     691     443     408     299     288     282     202
                  78      37      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     754     786    1785    1817    2046    2078    2721    2753 
   Size:        754      32     999      32     229      32     643      32

Cuts at:   2753    2887    2919    3073    3105    3628    3658    3660    3690 
   Size:        134      32     154      32     523      30       2      30

Cuts at:   3690    4108    4140    4594    4626    5067    5099    5121 
   Size:        418      32     454      32     441      32      22

  Fragments arranged by size: 

                 999     754     643     523     454     441     418     229
                 154     134      32      32      32      32      32      32
                  32      32      32      30      30      22       2
 
HincII GTy'rAC

Cuts at:      0     147    2060    5121 
   Size:        147    1913    3061

  Fragments arranged by size: 

                3061    1913     147
 
HindIII A'AGCT_T

Cuts at:      0     741    5121 
   Size:        741    4380
 
HpaI GTT'AAC

Cuts at:      0    2060    5121 
   Size:       2060    3061
 
KasI G'GCGC_C

Cuts at:      0     439    4159    4196    5121 
   Size:        439    3720      37     925

  Fragments arranged by size: 

                3720     925     439      37
 
KpnI G_GTAC'C

Cuts at:      0    4646    5121 
   Size:       4646     475
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1781    2990    5121 
   Size:       1781    1209    2131

  Fragments arranged by size: 

                2131    1781    1209
 
MscI TGG'CCA

Cuts at:      0     782    4924    5121 
   Size:        782    4142     197

  Fragments arranged by size: 

                4142     782     197
 
MslI CAynn'nnrTG

Cuts at:      0     140     697    1477    1967    2257    3520    3902    5023 
   Size:        140     557     780     490     290    1263     382    1121

Cuts at:   5023    5121 
   Size:         98

  Fragments arranged by size: 

                1263    1121     780     557     490     382     290     140
                  98
 
MspA1I CmG'CkG

Cuts at:      0     673    1204    2076    2110    3994    4071    4560    4620 
   Size:        673     531     872      34    1884      77     489      60

Cuts at:   4620    5121 
   Size:        501

  Fragments arranged by size: 

                1884     872     673     531     501     489      77      60
                  34
 
NaeI GCC'GGC

Cuts at:      0    1255    5121 
   Size:       1255    3866
 
NarI GG'CG_CC

Cuts at:      0     440    4160    4197    5121 
   Size:        440    3720      37     924

  Fragments arranged by size: 

                3720     924     440      37
 
NcoI C'CATG_G

Cuts at:      0    4751    5121 
   Size:       4751     370
 
NgoMIV G'CCGG_C

Cuts at:      0    1253    5121 
   Size:       1253    3868
 
NspI r_CATG'y

Cuts at:      0    4827    5121 
   Size:       4827     294
 
PasI CC'CwG_GG

Cuts at:      0    2318    2853    5121 
   Size:       2318     535    2268

  Fragments arranged by size: 

                2318    2268     535
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1318    3412    5121 
   Size:       1318    2094    1709

  Fragments arranged by size: 

                2094    1709    1318
 
PfoI T'CCnGG_A

Cuts at:      0    2396    3890    4004    5121 
   Size:       2396    1494     114    1117

  Fragments arranged by size: 

                2396    1494    1117     114
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1349    1381    5121 
   Size:       1349      32    3740

  Fragments arranged by size: 

                3740    1349      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    4002    5121 
   Size:       4002    1119
 
PspOMI G'GGCC_C

Cuts at:      0    1342    1609    2657    3415    4453    5121 
   Size:       1342     267    1048     758    1038     668

  Fragments arranged by size: 

                1342    1048    1038     758     668     267
 
PstI C_TGCA'G

Cuts at:      0     996    3357    4064    4355    5121 
   Size:        996    2361     707     291     766

  Fragments arranged by size: 

                2361     996     766     707     291
 
PvuII CAG'CTG

Cuts at:      0     673    1204    2076    2110    3994    4560    5121 
   Size:        673     531     872      34    1884     566     561

  Fragments arranged by size: 

                1884     872     673     566     561     531      34
 
RsrII CG'GwC_CG

Cuts at:      0    4610    5121 
   Size:       4610     511
 
SacI G_AGCT'C

Cuts at:      0    1994    5121 
   Size:       1994    3127
 
SapI GCTCTTCn'nnn_

Cuts at:      0     427     663    1737    2401    2831    3117    5121 
   Size:        427     236    1074     664     430     286    2004

  Fragments arranged by size: 

                2004    1074     664     430     427     286     236
 
SbfI CC_TGCA'GG

Cuts at:      0    3357    5121 
   Size:       3357    1764
 
SexAI A'CCwGG_T

Cuts at:      0    1317    5121 
   Size:       1317    3804
 
SfcI C'TryA_G

Cuts at:      0     992    1658    3353    4060    4351    5121 
   Size:        992     666    1695     707     291     770

  Fragments arranged by size: 

                1695     992     770     707     666     291
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3775    5121 
   Size:       3775    1346
 
SfoI GGC'GCC

Cuts at:      0     441    4161    4198    5121 
   Size:        441    3720      37     923

  Fragments arranged by size: 

                3720     923     441      37
 
SmaI CCC'GGG

Cuts at:      0    1687    3601    4459    5121 
   Size:       1687    1914     858     662

  Fragments arranged by size: 

                1914    1687     858     662
 
SmlI C'TyrA_G

Cuts at:      0    4687    5121 
   Size:       4687     434
 
SnaBI TAC'GTA

Cuts at:      0    4337    5121 
   Size:       4337     784
 
StuI AGG'CCT

Cuts at:      0     102    2140    2239    2733    3351    5121 
   Size:        102    2038      99     494     618    1770

  Fragments arranged by size: 

                2038    1770     618     494     102      99
 
StyI C'CwwG_G

Cuts at:      0      80     443     612     804    1560    1697    1985    2534 
   Size:         80     363     169     192     756     137     288     549

Cuts at:   2534    3865    4751    4964    5121 
   Size:       1331     886     213     157

  Fragments arranged by size: 

                1331     886     756     549     363     288     213     192
                 169     157     137      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1920    2015    2331    3495    5121 
   Size:       1920      95     316    1164    1626

  Fragments arranged by size: 

                1920    1626    1164     316      95
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     457     489    1608    1640    2854    2886    5121 
   Size:        457      32    1119      32    1214      32    2235

  Fragments arranged by size: 

                2235    1214    1119     457      32      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1685    3599    4457    5121 
   Size:       1685    1914     858     664

  Fragments arranged by size: 

                1914    1685     858     664
 
XmnI GAAnn'nnTTC

Cuts at:      0    1364    5121 
   Size:       1364    3757
 
ZraI GAC'GTC

Cuts at:      0    2806    5121 
   Size:       2806    2315
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI
    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII    RsrII     SacI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
    SnaBI     StuI     StyI    TaqII     TstI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII     AgeI     AhdI     AloI    ApaLI     AscI
     AseI    AsiSI     BaeI     BclI    BfrBI     BmtI     BsmI    BspHI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I    CspCI     DraI
    EcoRI    EcoRV     FseI    FspAI     MfeI     MluI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    SacII     SalI    SanDI     ScaI
     SgfI    SgrAI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI
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