Restriction map for
pF1KA0311
(Circular) (Six-base) MAPSORT of: pF1KA0311.seq Check: 4140 from: 1 to: 10070
pF1KA0311 10070 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 3510 3510
Size: 10070
AccI GT'mk_AC
Cuts at: 1010 2594 2917 3669 7108 1010
Size: 1584 323 752 3439 3972
Fragments arranged by size:
3972 3439 1584 752 323
Acc65I G'GTAC_C
Cuts at: 7086 7086
Size: 10070
AclI AA'CG_TT
Cuts at: 9910 9910
Size: 10070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1076 2698 5420 6905 7895 8327 9074 9342
Size: 387 1622 2722 1485 990 432 747 268
Cuts at: 9342 689
Size: 1417
Fragments arranged by size:
2722 1622 1485 1417 990 747 432 387
268
AflII C'TTAA_G
Cuts at: 2017 2017
Size: 10070
AflIII A'CryG_T
Cuts at: 3664 4328 5670 7422 8526 9503 3664
Size: 664 1342 1752 1104 977 4231
Fragments arranged by size:
4231 1752 1342 1104 977 664
AgeI A'CCGG_T
Cuts at: 8458 8458
Size: 10070
AhdI GACnn_n'nnGTC
Cuts at: 3042 3042
Size: 10070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 488 6532 488
Size: 6044 4026
AlwNI CAG_nnn'CTG
Cuts at: 153 292 979 2140 2397 5287 6818 7375 8942
Size: 139 687 1161 257 2890 1531 557 1567
Cuts at: 8942 153
Size: 1281
Fragments arranged by size:
2890 1567 1531 1281 1161 687 557 257
139
ApaI G_GGCC'C
Cuts at: 2255 2255
Size: 10070
ApaLI G'TGCA_C
Cuts at: 8840 8840
Size: 10070
ApoI r'AATT_y
Cuts at: 1522 1538 1746 2130 2476 4182 4342 5459 5700
Size: 16 208 384 346 1706 160 1117 241
Cuts at: 5700 5959 7074 9423 9679 1522
Size: 259 1115 2349 256 1913
Fragments arranged by size:
2349 1913 1706 1117 1115 384 346 259
256 241 208 160 16
AseI AT'TA_AT
Cuts at: 20 3230 20
Size: 3210 6860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10070
AvaI C'yCGr_G
Cuts at: 6537 7090 6537
Size: 553 9517
AvrII C'CTAG_G
Cuts at: 3261 3261
Size: 10070
BamHI G'GATC_C
Cuts at: 156 4593 7095 156
Size: 4437 2502 3131
Fragments arranged by size:
4437 3131 2502
BanI G'GyrC_C
Cuts at: 560 1620 1890 7086 7500 7748 7783 560
Size: 1060 270 5196 414 248 35 2847
Fragments arranged by size:
5196 2847 1060 414 270 248 35
BanII G_rGCy'C
Cuts at: 1687 2255 2763 3020 5041 5198 7084 8114 1687
Size: 568 508 257 2021 157 1886 1030 3643
Fragments arranged by size:
3643 2021 1886 1030 568 508 257 157
BbeI G_GCGC'C
Cuts at: 7504 7752 7504
Size: 248 9822
BbsI GAAGACnn'nnnn_
Cuts at: 1040 1427 5159 5474 1040
Size: 387 3732 315 5636
Fragments arranged by size:
5636 3732 387 315
BbvCI CC'TCA_GC
Cuts at: 2840 6773 2840
Size: 3933 6137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 550 584 3490 3524 6334 6368 550
Size: 34 2906 34 2810 34 4252
Fragments arranged by size:
4252 2906 2810 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2760 7968 8729 10070 2760
Size: 5208 761 1341 2760
Fragments arranged by size:
5208 2760 1341 761
BclI T'GATC_A
Cuts at: 453 3602 3822 7590 453
Size: 3149 220 3768 2933
Fragments arranged by size:
3768 3149 2933 220
BfrBI ATG'CAT
Cuts at: 5254 7059 5254
Size: 1805 8265
BglII A'GATC_T
Cuts at: 4119 4929 5007 7585 9622 4119
Size: 810 78 2578 2037 4567
Fragments arranged by size:
4567 2578 2037 810 78
BlpI GC'TnA_GC
Cuts at: 1686 7182 1686
Size: 5496 4574
Bme1580I G_kGCm'C
Cuts at: 2255 3296 7695 7788 8844 2255
Size: 1041 4399 93 1056 3481
Fragments arranged by size:
4399 3481 1056 1041 93
BmgBI CAC'GTC
Cuts at: 1947 3667 1947
Size: 1720 8350
BmrI ACTGGGnnnn_n'
Cuts at: 1084 2121 7282 7363 7698 9842 1084
Size: 1037 5161 81 335 2144 1312
Fragments arranged by size:
5161 2144 1312 1037 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6612 8437 6612
Size: 1825 8245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2584 3376 4808 4861 5361 5421 2584
Size: 792 1432 53 500 60 7233
Fragments arranged by size:
7233 1432 792 500 60 53
Bpu10I CC'TnA_GC
Cuts at: 905 2840 6773 905
Size: 1935 3933 4202
Fragments arranged by size:
4202 3933 1935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1789 2595 2596 2668 2881 7246 8125 8617
Size: 812 806 1 72 213 4365 879 492
Cuts at: 8617 8915 977
Size: 298 2132
Fragments arranged by size:
4365 2132 879 812 806 492 298 213
72 1
BsaI GGTCTCn'nnnn_
Cuts at: 322 356 5638 9774 322
Size: 34 5282 4136 618
Fragments arranged by size:
5282 4136 618 34
BsaAI yAC'GTr
Cuts at: 3313 7423 3313
Size: 4110 5960
BsaBI GATnn'nnATC
Cuts at: 6 413 7584 9621 6
Size: 407 7171 2037 455
Fragments arranged by size:
7171 2037 455 407
BsaHI Gr'CG_yC
Cuts at: 620 3507 7501 7749 8451 9947 620
Size: 2887 3994 248 702 1496 743
Fragments arranged by size:
3994 2887 1496 743 702 248
BsaWI w'CCGG_w
Cuts at: 338 1887 6874 7259 7780 8458 8732 8879 9290
Size: 1549 4987 385 521 678 274 147 411
Cuts at: 9290 338
Size: 1118
Fragments arranged by size:
4987 1549 1118 678 521 411 385 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 867 897 1963 1993 4307 4337 5359 5389 6373
Size: 30 1066 30 2314 30 1022 30 984
Cuts at: 6373 6403 867
Size: 30 4534
Fragments arranged by size:
4534 2314 1066 1022 984 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 997 1116 1149 1923 2099 2293 2641 3097
Size: 453 119 33 774 176 194 348 456
Cuts at: 3097 3527 3910 4181 6620 6622 544
Size: 430 383 271 2439 2 3992
Fragments arranged by size:
3992 2439 774 456 453 430 383 348
271 194 176 119 33 2
BseYI C'CCAG_C
Cuts at: 6652 6991 7495 8253 8830 9326 6652
Size: 339 504 758 577 496 7396
Fragments arranged by size:
7396 758 577 504 496 339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1159 5457 6842 568
Size: 591 4298 1385 3796
Fragments arranged by size:
4298 3796 1385 591
BsiEI CG_ry'CG
Cuts at: 104 7612 7658 8866 104
Size: 7508 46 1208 1308
Fragments arranged by size:
7508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1687 2610 3020 5198 6630 7084 7862 8052 8844
Size: 923 410 2178 1432 454 778 190 792
Cuts at: 8844 1687
Size: 2913
Fragments arranged by size:
2913 2178 1432 923 792 778 454 410
190
BsmI GAATG_Cn'
Cuts at: 599 778 1800 3582 4776 5256 6396 6571 6902
Size: 179 1022 1782 1194 480 1140 175 331
Cuts at: 6902 599
Size: 3767
Fragments arranged by size:
3767 1782 1194 1140 1022 480 331 179
175
Bsp1286I G_dGCh'C
Cuts at: 1687 2255 2610 2763 3020 3296 5041 5198 6630
Size: 568 355 153 257 276 1745 157 1432
Cuts at: 6630 7084 7695 7788 7862 8052 8114 8844 1687
Size: 454 611 93 74 190 62 730 2913
Fragments arranged by size:
2913 1745 1432 730 611 568 454 355
276 257 190 157 153 93 74 62
BspEI T'CCGG_A
Cuts at: 338 6874 9290 338
Size: 6536 2416 1118
Fragments arranged by size:
6536 2416 1118
BspHI T'CATG_A
Cuts at: 1167 4707 6459 7035 9246 1167
Size: 3540 1752 576 2211 1991
Fragments arranged by size:
3540 2211 1991 1752 576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3326 5378 7120 7334 7636 8017 3326
Size: 2052 1742 214 302 381 5379
Fragments arranged by size:
5379 2052 1742 381 302 214
BsrBI CCG'CTC
Cuts at: 50 8362 8416 9900 10065 50
Size: 8312 54 1484 165 55
Fragments arranged by size:
8312 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 239 1270 1335 2988 4929 5571 6514 6671 6982
Size: 1031 65 1653 1941 642 943 157 311
Cuts at: 6982 7982 239
Size: 1000 2327
Fragments arranged by size:
2327 1941 1653 1031 1000 943 642 311
157 65
BsrFI r'CCGG_y
Cuts at: 686 6404 8068 8249 8458 686
Size: 5718 1664 181 209 2298
Fragments arranged by size:
5718 2298 1664 209 181
BsrGI T'GTAC_A
Cuts at: 411 2903 3971 411
Size: 2492 1068 6510
Fragments arranged by size:
6510 2492 1068
BssSI C'ACGA_G
Cuts at: 8341 8699 8341
Size: 358 9712
BstAPI GCAn_nnn'nTGC
Cuts at: 612 7447 612
Size: 6835 3235
BstBI TT'CG_AA
Cuts at: 782 8431 782
Size: 7649 2421
BstEII G'GTnAC_C
Cuts at: 6480 6480
Size: 10070
BstYI r'GATC_y
Cuts at: 1 156 1395 1408 3272 3916 4119 4593 4929
Size: 155 1239 13 1864 644 203 474 336
Cuts at: 4929 5007 5237 5769 7095 7562 7585 7920 8166
Size: 78 230 532 1326 467 23 335 246
Cuts at: 8166 9178 9264 9276 9622 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1864 1326 1239 1012 644 532 474 467
449 346 336 335 246 230 203 155
86 78 23 13 12
BstZ17I GTA'TAC
Cuts at: 2918 2918
Size: 10070
Bsu36I CC'TnA_GG
Cuts at: 139 139
Size: 10070
BtgI C'CryG_G
Cuts at: 161 182 2501 3909 161
Size: 21 2319 1408 6322
Fragments arranged by size:
6322 2319 1408 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5312 8020 8201 8364 5312
Size: 2708 181 163 7018
Fragments arranged by size:
7018 2708 181 163
BtsI GCAGTG_nn'
Cuts at: 551 972 1697 1857 2002 4197 4268 4872 4977
Size: 421 725 160 145 2195 71 604 105
Cuts at: 4977 5193 5613 6029 7313 551
Size: 216 420 416 1284 3308
Fragments arranged by size:
3308 2195 1284 725 604 421 420 416
216 160 145 105 71
DraI TTT'AAA
Cuts at: 4436 4857 7069 4436
Size: 421 2212 7437
Fragments arranged by size:
7437 2212 421
DraIII CAC_nnn'GTG
Cuts at: 546 1491 546
Size: 945 9125
DrdI GACnn_nn'nnGTC
Cuts at: 7776 8634 7776
Size: 858 9212
EaeI y'GGCC_r
Cuts at: 185 5115 7655 7829 8220 8247 9592 185
Size: 4930 2540 174 391 27 1345 663
Fragments arranged by size:
4930 2540 1345 663 391 174 27
EagI C'GGCC_G
Cuts at: 7655 7655
Size: 10070
EarI CTCTTCn'nnn_
Cuts at: 221 665 1049 1610 2229 2936 4095 5087 5399
Size: 444 384 561 619 707 1159 992 312
Cuts at: 5399 6491 8093 8303 221
Size: 1092 1602 210 1988
Fragments arranged by size:
1988 1602 1159 1092 992 707 619 561
444 384 312 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8278 8588 8734 9880 8278
Size: 310 146 1146 8468
Fragments arranged by size:
8468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1685 3018 5196 7082 1685
Size: 1333 2178 1886 4673
Fragments arranged by size:
4673 2178 1886 1333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1076 2584 2698 3376 4808 4861 5361 5420
Size: 387 1508 114 678 1432 53 500 59
Cuts at: 5420 5421 6905 7895 8327 9074 9342 689
Size: 1 1484 990 432 747 268 1417
Fragments arranged by size:
1508 1484 1432 1417 990 747 678 500
432 387 268 114 59 53 1
EcoNI CCTnn'n_nnAGG
Cuts at: 351 5523 351
Size: 5172 4898
EcoO109I rG'GnC_Cy
Cuts at: 150 2252 3879 4271 7209 150
Size: 2102 1627 392 2938 3011
Fragments arranged by size:
3011 2938 2102 1627 392
EcoRI G'AATT_C
Cuts at: 2130 2476 4182 5700 7074 2130
Size: 346 1706 1518 1374 5126
Fragments arranged by size:
5126 1706 1518 1374 346
EcoRV GAT'ATC
Cuts at: 5076 5076
Size: 10070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1400 4907 5124 7039 8089 1400
Size: 3507 217 1915 1050 3381
Fragments arranged by size:
3507 3381 1915 1050 217
FspI TGC'GCA
Cuts at: 4155 7851 4155
Size: 3696 6374
HaeII r_GCGC'y
Cuts at: 7504 7752 8774 9753 7504
Size: 248 1022 979 7821
Fragments arranged by size:
7821 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 823 855 937 969 973 1005 2078
Size: 32 559 32 82 32 4 32 1073
Cuts at: 2078 2110 2206 2238 2266 2298 2311 2343 2688
Size: 32 96 32 28 32 13 32 345
Cuts at: 2688 2720 2875 2907 3028 3060 3257 3289 3307
Size: 32 155 32 121 32 197 32 18
Cuts at: 3307 3339 3555 3587 4612 4644 4835 4867 5122
Size: 32 216 32 1025 32 191 32 255
Cuts at: 5122 5154 5182 5214 5359 5391 5419 5451 5604
Size: 32 28 32 145 32 28 32 153
Cuts at: 5604 5636 7912 7942 7944 7974 232
Size: 32 2276 30 2 30 2328
Fragments arranged by size:
2328 2276 1073 1025 559 345 255 216
197 191 155 153 145 121 96 82
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 28
28 28 18 13 4 2
HincII GTy'rAC
Cuts at: 113 522 4326 4907 7109 113
Size: 409 3804 581 2202 3074
Fragments arranged by size:
3804 3074 2202 581 409
HindIII A'AGCT_T
Cuts at: 659 2062 3170 3856 4369 6850 659
Size: 1403 1108 686 513 2481 3879
Fragments arranged by size:
3879 2481 1403 1108 686 513
HpaI GTT'AAC
Cuts at: 113 4326 113
Size: 4213 5857
KasI G'GCGC_C
Cuts at: 7500 7748 7500
Size: 248 9822
KpnI G_GTAC'C
Cuts at: 7090 7090
Size: 10070
MfeI C'AATT_G
Cuts at: 1807 1807
Size: 10070
MluI A'CGCG_T
Cuts at: 9503 9503
Size: 10070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1771 2027 2176 3858 4407 6371 6759 8741 8925
Size: 256 149 1682 549 1964 388 1982 184
Cuts at: 8925 1771
Size: 2916
Fragments arranged by size:
2916 1982 1964 1682 549 388 256 184
149
MscI TGG'CCA
Cuts at: 187 5117 7831 187
Size: 4930 2714 2426
Fragments arranged by size:
4930 2714 2426
MslI CAynn'nnrTG
Cuts at: 121 193 407 1169 3640 4045 4762 5479 5714
Size: 72 214 762 2471 405 717 717 235
Cuts at: 5714 6301 6464 8059 8186 121
Size: 587 163 1595 127 2005
Fragments arranged by size:
2471 2005 1595 762 717 717 587 405
235 214 163 127 72
MspA1I CmG'CkG
Cuts at: 2747 3354 3600 5161 6417 6721 7181 7495 7855
Size: 607 246 1561 1256 304 460 314 360
Cuts at: 7855 8868 9113 2747
Size: 1013 245 3704
Fragments arranged by size:
3704 1561 1256 1013 607 460 360 314
304 246 245
NaeI GCC'GGC
Cuts at: 688 8251 688
Size: 7563 2507
NarI GG'CG_CC
Cuts at: 7501 7749 7501
Size: 248 9822
NcoI C'CATG_G
Cuts at: 161 182 2501 3909 161
Size: 21 2319 1408 6322
Fragments arranged by size:
6322 2319 1408 21
NdeI CA'TA_TG
Cuts at: 2437 5775 2437
Size: 3338 6732
NgoMIV G'CCGG_C
Cuts at: 686 8249 686
Size: 7563 2507
NsiI A_TGCA'T
Cuts at: 5256 7061 5256
Size: 1805 8265
NspI r_CATG'y
Cuts at: 406 2688 4014 4160 4588 4767 6689 7123 7623
Size: 2282 1326 146 428 179 1922 434 500
Cuts at: 7623 8530 406
Size: 907 1946
Fragments arranged by size:
2282 1946 1922 1326 907 500 434 428
179 146
PciI A'CATG_T
Cuts at: 8526 8526
Size: 10070
PfoI T'CCnGG_A
Cuts at: 3275 5240 3275
Size: 1965 8105
PmeI GTTT'AAAC
Cuts at: 7069 7069
Size: 10070
PmlI CAC'GTG
Cuts at: 3313 3313
Size: 10070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9209 9241 9209
Size: 32 10038
PpuMI rG'GwC_Cy
Cuts at: 150 3879 4271 150
Size: 3729 392 5949
Fragments arranged by size:
5949 3729 392
PshAI GACnn'nnGTC
Cuts at: 797 797
Size: 10070
PsiI TTA'TAA
Cuts at: 1244 1244
Size: 10070
PspOMI G'GGCC_C
Cuts at: 2251 2251
Size: 10070
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 404 436 3435 3467 404
Size: 32 2999 32 7007
Fragments arranged by size:
7007 2999 32 32
PstI C_TGCA'G
Cuts at: 617 1050 2723 6648 7117 7802 617
Size: 433 1673 3925 469 685 2885
Fragments arranged by size:
3925 2885 1673 685 469 433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10070
PvuII CAG'CTG
Cuts at: 2747 3354 3600 6417 6721 7495 7855 2747
Size: 607 246 2817 304 774 360 4962
Fragments arranged by size:
4962 2817 774 607 360 304 246
RsrII CG'GwC_CG
Cuts at: 8265 8265
Size: 10070
SacI G_AGCT'C
Cuts at: 1687 3020 5198 7084 1687
Size: 1333 2178 1886 4673
Fragments arranged by size:
4673 2178 1886 1333
SalI G'TCGA_C
Cuts at: 7107 7107
Size: 10070
SanDI GG'GwC_CC
Cuts at: 4271 4271
Size: 10070
SapI GCTCTTCn'nnn_
Cuts at: 1049 5399 8093 8303 1049
Size: 4350 2694 210 2816
Fragments arranged by size:
4350 2816 2694 210
SbfI CC_TGCA'GG
Cuts at: 7117 7117
Size: 10070
ScaI AGT'ACT
Cuts at: 9587 9587
Size: 10070
SexAI A'CCwGG_T
Cuts at: 3965 6079 6882 3965
Size: 2114 803 7153
Fragments arranged by size:
7153 2114 803
SfcI C'TryA_G
Cuts at: 33 613 795 879 1046 2510 2719 4652 6644
Size: 580 182 84 167 1464 209 1933 1992
Cuts at: 6644 7113 7798 8791 8982 33
Size: 469 685 993 191 1121
Fragments arranged by size:
1992 1933 1464 1121 993 685 580 469
209 191 182 167 84
SfoI GGC'GCC
Cuts at: 7502 7750 7502
Size: 248 9822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10070
SmaI CCC'GGG
Cuts at: 7092 7092
Size: 10070
SmlI C'TyrA_G
Cuts at: 992 1768 2017 2574 2611 2647 2896 7225 8140
Size: 776 249 557 37 36 249 4329 915
Cuts at: 8140 8632 8894 992
Size: 492 262 2168
Fragments arranged by size:
4329 2168 915 776 557 492 262 249
249 37 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10070
SphI G_CATG'C
Cuts at: 4767 7123 7623 4767
Size: 2356 500 7214
Fragments arranged by size:
7214 2356 500
SspI AAT'ATT
Cuts at: 2103 3936 5640 5802 2103
Size: 1833 1704 162 6371
Fragments arranged by size:
6371 1833 1704 162
StuI AGG'CCT
Cuts at: 1975 6580 1975
Size: 4605 5465
StyI C'CwwG_G
Cuts at: 161 182 1721 2501 2834 3261 3909 4527 7204
Size: 21 1539 780 333 427 648 618 2677
Cuts at: 7204 7558 161
Size: 354 2673
Fragments arranged by size:
2677 2673 1539 780 648 618 427 354
333 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 195 216 7653 7786 8245 8454 195
Size: 21 7437 133 459 209 1811
Fragments arranged by size:
7437 1811 459 209 133 21
TatI w'GTAC_w
Cuts at: 411 2903 3971 6273 9585 411
Size: 2492 1068 2302 3312 896
Fragments arranged by size:
3312 2492 2302 1068 896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 867 899 5718 5750 6773 6805 9587 9619 867
Size: 32 4819 32 1023 32 2782 32 1318
Fragments arranged by size:
4819 2782 1318 1023 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7867 7867
Size: 10070
XbaI T'CTAG_A
Cuts at: 7101 7101
Size: 10070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1419 4401 6811 7565 1419
Size: 2982 2410 754 3924
Fragments arranged by size:
3924 2982 2410 754
XmaI C'CCGG_G
Cuts at: 7090 7090
Size: 10070
XmnI GAAnn'nnTTC
Cuts at: 2480 4887 5067 5810 6055 7022 2480
Size: 2407 180 743 245 967 5528
Fragments arranged by size:
5528 2407 967 743 245 180
ZraI GAC'GTC
Cuts at: 3508 3508
Size: 10070
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AleI AloI AscI BaeI BglI BmtI
BsiWI BsmBI BssHII BstXI ClaI CspCI FseI FspAI
NheI NotI NruI PacI PasI PflMI PspXI SacII
SfiI SgrAI SnaBI SrfI SwaI XhoI