Restriction map for
pF1KA0311
(Linear) (Six-base) MAPSORT of: KIAA0311.seq Check: 9966 from: 1 to: 6957
KIAA0311.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AatII G_ACGT'C
Cuts at: 0 3402 6957
Size: 3402 3555
AccI GT'mk_AC
Cuts at: 0 902 2486 2809 3561 6957
Size: 902 1584 323 752 3396
Fragments arranged by size:
3396 1584 902 752 323
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 968 2590 5312 6797 6957
Size: 581 387 1622 2722 1485 160
Fragments arranged by size:
2722 1622 1485 581 387 160
AflII C'TTAA_G
Cuts at: 0 1909 6957
Size: 1909 5048
AflIII A'CryG_T
Cuts at: 0 3556 4220 5562 6957
Size: 3556 664 1342 1395
Fragments arranged by size:
3556 1395 1342 664
AhdI GACnn_n'nnGTC
Cuts at: 0 2934 6957
Size: 2934 4023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 380 6424 6957
Size: 380 6044 533
Fragments arranged by size:
6044 533 380
AlwNI CAG_nnn'CTG
Cuts at: 0 45 184 871 2032 2289 5179 6710 6957
Size: 45 139 687 1161 257 2890 1531 247
Fragments arranged by size:
2890 1531 1161 687 257 247 139 45
ApaI G_GGCC'C
Cuts at: 0 2147 6957
Size: 2147 4810
ApoI r'AATT_y
Cuts at: 0 1414 1430 1638 2022 2368 4074 4234 5351
Size: 1414 16 208 384 346 1706 160 1117
Cuts at: 5351 5592 5851 6957
Size: 241 259 1106
Fragments arranged by size:
1706 1414 1117 1106 384 346 259 241
208 160 16
AseI AT'TA_AT
Cuts at: 0 3122 6957
Size: 3122 3835
AvaI C'yCGr_G
Cuts at: 0 6429 6957
Size: 6429 528
AvrII C'CTAG_G
Cuts at: 0 3153 6957
Size: 3153 3804
BamHI G'GATC_C
Cuts at: 0 48 4485 6957
Size: 48 4437 2472
Fragments arranged by size:
4437 2472 48
BanI G'GyrC_C
Cuts at: 0 452 1512 1782 6957
Size: 452 1060 270 5175
Fragments arranged by size:
5175 1060 452 270
BanII G_rGCy'C
Cuts at: 0 1579 2147 2655 2912 4933 5090 6957
Size: 1579 568 508 257 2021 157 1867
Fragments arranged by size:
2021 1867 1579 568 508 257 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 932 1319 5051 5366 6957
Size: 932 387 3732 315 1591
Fragments arranged by size:
3732 1591 932 387 315
BbvCI CC'TCA_GC
Cuts at: 0 2732 6665 6957
Size: 2732 3933 292
Fragments arranged by size:
3933 2732 292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 476 3382 3416 6226 6260 6957
Size: 442 34 2906 34 2810 34 697
Fragments arranged by size:
2906 2810 697 442 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2652 6957
Size: 2652 4305
BclI T'GATC_A
Cuts at: 0 345 3494 3714 6957
Size: 345 3149 220 3243
Fragments arranged by size:
3243 3149 345 220
BfrBI ATG'CAT
Cuts at: 0 5146 6951 6957
Size: 5146 1805 6
Fragments arranged by size:
5146 1805 6
BglII A'GATC_T
Cuts at: 0 4011 4821 4899 6957
Size: 4011 810 78 2058
Fragments arranged by size:
4011 2058 810 78
BlpI GC'TnA_GC
Cuts at: 0 1578 6957
Size: 1578 5379
Bme1580I G_kGCm'C
Cuts at: 0 2147 3188 6957
Size: 2147 1041 3769
Fragments arranged by size:
3769 2147 1041
BmgBI CAC'GTC
Cuts at: 0 1839 3559 6957
Size: 1839 1720 3398
Fragments arranged by size:
3398 1839 1720
BmrI ACTGGGnnnn_n'
Cuts at: 0 976 2013 6957
Size: 976 1037 4944
Fragments arranged by size:
4944 1037 976
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6504 6957
Size: 6504 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2476 3268 4700 4753 5253 5313 6957
Size: 2476 792 1432 53 500 60 1644
Fragments arranged by size:
2476 1644 1432 792 500 60 53
Bpu10I CC'TnA_GC
Cuts at: 0 797 2732 6665 6957
Size: 797 1935 3933 292
Fragments arranged by size:
3933 1935 797 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1681 2487 2488 2560 2773 6957
Size: 869 812 806 1 72 213 4184
Fragments arranged by size:
4184 869 812 806 213 72 1
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 248 5530 6957
Size: 214 34 5282 1427
Fragments arranged by size:
5282 1427 214 34
BsaAI yAC'GTr
Cuts at: 0 3205 6957
Size: 3205 3752
BsaBI GATnn'nnATC
Cuts at: 0 305 6957
Size: 305 6652
BsaHI Gr'CG_yC
Cuts at: 0 512 3399 6957
Size: 512 2887 3558
Fragments arranged by size:
3558 2887 512
BsaWI w'CCGG_w
Cuts at: 0 230 1779 6766 6957
Size: 230 1549 4987 191
Fragments arranged by size:
4987 1549 230 191
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 759 789 1855 1885 4199 4229 5251 5281
Size: 759 30 1066 30 2314 30 1022 30
Cuts at: 5281 6265 6295 6957
Size: 984 30 662
Fragments arranged by size:
2314 1066 1022 984 759 662 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 889 1008 1041 1815 1991 2185 2533
Size: 436 453 119 33 774 176 194 348
Cuts at: 2533 2989 3419 3802 4073 6512 6514 6957
Size: 456 430 383 271 2439 2 443
Fragments arranged by size:
2439 774 456 453 443 436 430 383
348 271 194 176 119 33 2
BseYI C'CCAG_C
Cuts at: 0 6544 6883 6957
Size: 6544 339 74
Fragments arranged by size:
6544 339 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1051 5349 6734 6957
Size: 460 591 4298 1385 223
Fragments arranged by size:
4298 1385 591 460 223
BsiHKAI G_wGCw'C
Cuts at: 0 1579 2502 2912 5090 6522 6957
Size: 1579 923 410 2178 1432 435
Fragments arranged by size:
2178 1579 1432 923 435 410
BsmI GAATG_Cn'
Cuts at: 0 491 670 1692 3474 4668 5148 6288 6463
Size: 491 179 1022 1782 1194 480 1140 175
Cuts at: 6463 6794 6957
Size: 331 163
Fragments arranged by size:
1782 1194 1140 1022 491 480 331 179
175 163
Bsp1286I G_dGCh'C
Cuts at: 0 1579 2147 2502 2655 2912 3188 4933 5090
Size: 1579 568 355 153 257 276 1745 157
Cuts at: 5090 6522 6957
Size: 1432 435
Fragments arranged by size:
1745 1579 1432 568 435 355 276 257
157 153
BspEI T'CCGG_A
Cuts at: 0 230 6766 6957
Size: 230 6536 191
Fragments arranged by size:
6536 230 191
BspHI T'CATG_A
Cuts at: 0 1059 4599 6351 6927 6957
Size: 1059 3540 1752 576 30
Fragments arranged by size:
3540 1752 1059 576 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3218 5270 6957
Size: 3218 2052 1687
Fragments arranged by size:
3218 2052 1687
BsrDI GCAATG_nn'
Cuts at: 0 131 1162 1227 2880 4821 5463 6406 6563
Size: 131 1031 65 1653 1941 642 943 157
Cuts at: 6563 6874 6957
Size: 311 83
Fragments arranged by size:
1941 1653 1031 943 642 311 157 131
83 65
BsrFI r'CCGG_y
Cuts at: 0 578 6296 6957
Size: 578 5718 661
Fragments arranged by size:
5718 661 578
BsrGI T'GTAC_A
Cuts at: 0 303 2795 3863 6957
Size: 303 2492 1068 3094
Fragments arranged by size:
3094 2492 1068 303
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 6957
Size: 504 6453
BstBI TT'CG_AA
Cuts at: 0 674 6957
Size: 674 6283
BstEII G'GTnAC_C
Cuts at: 0 6372 6957
Size: 6372 585
BstYI r'GATC_y
Cuts at: 0 48 1287 1300 3164 3808 4011 4485 4821
Size: 48 1239 13 1864 644 203 474 336
Cuts at: 4821 4899 5129 5661 6957
Size: 78 230 532 1296
Fragments arranged by size:
1864 1296 1239 644 532 474 336 230
203 78 48 13
BstZ17I GTA'TAC
Cuts at: 0 2810 6957
Size: 2810 4147
Bsu36I CC'TnA_GG
Cuts at: 0 31 6957
Size: 31 6926
BtgI C'CryG_G
Cuts at: 0 53 74 2393 3801 6957
Size: 53 21 2319 1408 3156
Fragments arranged by size:
3156 2319 1408 53 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5204 6957
Size: 5204 1753
BtsI GCAGTG_nn'
Cuts at: 0 443 864 1589 1749 1894 4089 4160 4764
Size: 443 421 725 160 145 2195 71 604
Cuts at: 4764 4869 5085 5505 5921 6957
Size: 105 216 420 416 1036
Fragments arranged by size:
2195 1036 725 604 443 421 420 416
216 160 145 105 71
DraI TTT'AAA
Cuts at: 0 4328 4749 6957
Size: 4328 421 2208
Fragments arranged by size:
4328 2208 421
DraIII CAC_nnn'GTG
Cuts at: 0 438 1383 6957
Size: 438 945 5574
Fragments arranged by size:
5574 945 438
EaeI y'GGCC_r
Cuts at: 0 77 5007 6957
Size: 77 4930 1950
Fragments arranged by size:
4930 1950 77
EarI CTCTTCn'nnn_
Cuts at: 0 113 557 941 1502 2121 2828 3987 4979
Size: 113 444 384 561 619 707 1159 992
Cuts at: 4979 5291 6383 6957
Size: 312 1092 574
Fragments arranged by size:
1159 1092 992 707 619 574 561 444
384 312 113
EcoICRI GAG'CTC
Cuts at: 0 1577 2910 5088 6957
Size: 1577 1333 2178 1869
Fragments arranged by size:
2178 1869 1577 1333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 968 2476 2590 3268 4700 4753 5253
Size: 581 387 1508 114 678 1432 53 500
Cuts at: 5253 5312 5313 6797 6957
Size: 59 1 1484 160
Fragments arranged by size:
1508 1484 1432 678 581 500 387 160
114 59 53 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 243 5415 6957
Size: 243 5172 1542
Fragments arranged by size:
5172 1542 243
EcoO109I rG'GnC_Cy
Cuts at: 0 42 2144 3771 4163 6957
Size: 42 2102 1627 392 2794
Fragments arranged by size:
2794 2102 1627 392 42
EcoRI G'AATT_C
Cuts at: 0 2022 2368 4074 5592 6957
Size: 2022 346 1706 1518 1365
Fragments arranged by size:
2022 1706 1518 1365 346
EcoRV GAT'ATC
Cuts at: 0 4968 6957
Size: 4968 1989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1292 4799 5016 6931 6957
Size: 1292 3507 217 1915 26
Fragments arranged by size:
3507 1915 1292 217 26
FspI TGC'GCA
Cuts at: 0 4047 6957
Size: 4047 2910
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 715 747 829 861 865 897
Size: 124 32 559 32 82 32 4 32
Cuts at: 897 1970 2002 2098 2130 2158 2190 2203 2235
Size: 1073 32 96 32 28 32 13 32
Cuts at: 2235 2580 2612 2767 2799 2920 2952 3149 3181
Size: 345 32 155 32 121 32 197 32
Cuts at: 3181 3199 3231 3447 3479 4504 4536 4727 4759
Size: 18 32 216 32 1025 32 191 32
Cuts at: 4759 5014 5046 5074 5106 5251 5283 5311 5343
Size: 255 32 28 32 145 32 28 32
Cuts at: 5343 5496 5528 6957
Size: 153 32 1429
Fragments arranged by size:
1429 1073 1025 559 345 255 216 197
191 155 153 145 124 121 96 82
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 28 28 28
18 13 4
HincII GTy'rAC
Cuts at: 0 5 414 4218 4799 6957
Size: 5 409 3804 581 2158
Fragments arranged by size:
3804 2158 581 409 5
HindIII A'AGCT_T
Cuts at: 0 551 1954 3062 3748 4261 6742 6957
Size: 551 1403 1108 686 513 2481 215
Fragments arranged by size:
2481 1403 1108 686 551 513 215
HpaI GTT'AAC
Cuts at: 0 5 4218 6957
Size: 5 4213 2739
Fragments arranged by size:
4213 2739 5
MfeI C'AATT_G
Cuts at: 0 1699 6957
Size: 1699 5258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1663 1919 2068 3750 4299 6263 6651 6957
Size: 1663 256 149 1682 549 1964 388 306
Fragments arranged by size:
1964 1682 1663 549 388 306 256 149
MscI TGG'CCA
Cuts at: 0 79 5009 6957
Size: 79 4930 1948
Fragments arranged by size:
4930 1948 79
MslI CAynn'nnrTG
Cuts at: 0 13 85 299 1061 3532 3937 4654 5371
Size: 13 72 214 762 2471 405 717 717
Cuts at: 5371 5606 6193 6356 6957
Size: 235 587 163 601
Fragments arranged by size:
2471 762 717 717 601 587 405 235
214 163 72 13
MspA1I CmG'CkG
Cuts at: 0 2639 3246 3492 5053 6309 6613 6957
Size: 2639 607 246 1561 1256 304 344
Fragments arranged by size:
2639 1561 1256 607 344 304 246
NaeI GCC'GGC
Cuts at: 0 580 6957
Size: 580 6377
NcoI C'CATG_G
Cuts at: 0 53 74 2393 3801 6957
Size: 53 21 2319 1408 3156
Fragments arranged by size:
3156 2319 1408 53 21
NdeI CA'TA_TG
Cuts at: 0 2329 5667 6957
Size: 2329 3338 1290
Fragments arranged by size:
3338 2329 1290
NgoMIV G'CCGG_C
Cuts at: 0 578 6957
Size: 578 6379
NsiI A_TGCA'T
Cuts at: 0 5148 6953 6957
Size: 5148 1805 4
Fragments arranged by size:
5148 1805 4
NspI r_CATG'y
Cuts at: 0 298 2580 3906 4052 4480 4659 6581 6957
Size: 298 2282 1326 146 428 179 1922 376
Fragments arranged by size:
2282 1922 1326 428 376 298 179 146
PfoI T'CCnGG_A
Cuts at: 0 3167 5132 6957
Size: 3167 1965 1825
Fragments arranged by size:
3167 1965 1825
PmlI CAC'GTG
Cuts at: 0 3205 6957
Size: 3205 3752
PpuMI rG'GwC_Cy
Cuts at: 0 42 3771 4163 6957
Size: 42 3729 392 2794
Fragments arranged by size:
3729 2794 392 42
PshAI GACnn'nnGTC
Cuts at: 0 689 6957
Size: 689 6268
PsiI TTA'TAA
Cuts at: 0 1136 6957
Size: 1136 5821
PspOMI G'GGCC_C
Cuts at: 0 2143 6957
Size: 2143 4814
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 296 328 3327 3359 6957
Size: 296 32 2999 32 3598
Fragments arranged by size:
3598 2999 296 32 32
PstI C_TGCA'G
Cuts at: 0 509 942 2615 6540 6957
Size: 509 433 1673 3925 417
Fragments arranged by size:
3925 1673 509 433 417
PvuII CAG'CTG
Cuts at: 0 2639 3246 3492 6309 6613 6957
Size: 2639 607 246 2817 304 344
Fragments arranged by size:
2817 2639 607 344 304 246
SacI G_AGCT'C
Cuts at: 0 1579 2912 5090 6957
Size: 1579 1333 2178 1867
Fragments arranged by size:
2178 1867 1579 1333
SanDI GG'GwC_CC
Cuts at: 0 4163 6957
Size: 4163 2794
SapI GCTCTTCn'nnn_
Cuts at: 0 941 5291 6957
Size: 941 4350 1666
Fragments arranged by size:
4350 1666 941
SexAI A'CCwGG_T
Cuts at: 0 3857 5971 6774 6957
Size: 3857 2114 803 183
Fragments arranged by size:
3857 2114 803 183
SfcI C'TryA_G
Cuts at: 0 505 687 771 938 2402 2611 4544 6536
Size: 505 182 84 167 1464 209 1933 1992
Cuts at: 6536 6957
Size: 421
Fragments arranged by size:
1992 1933 1464 505 421 209 182 167
84
SmlI C'TyrA_G
Cuts at: 0 884 1660 1909 2466 2503 2539 2788 6957
Size: 884 776 249 557 37 36 249 4169
Fragments arranged by size:
4169 884 776 557 249 249 37 36
SphI G_CATG'C
Cuts at: 0 4659 6957
Size: 4659 2298
SspI AAT'ATT
Cuts at: 0 1995 3828 5532 5694 6957
Size: 1995 1833 1704 162 1263
Fragments arranged by size:
1995 1833 1704 1263 162
StuI AGG'CCT
Cuts at: 0 1867 6472 6957
Size: 1867 4605 485
Fragments arranged by size:
4605 1867 485
StyI C'CwwG_G
Cuts at: 0 53 74 1613 2393 2726 3153 3801 4419
Size: 53 21 1539 780 333 427 648 618
Cuts at: 4419 6957
Size: 2538
Fragments arranged by size:
2538 1539 780 648 618 427 333 53
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 108 6957
Size: 87 21 6849
Fragments arranged by size:
6849 87 21
TatI w'GTAC_w
Cuts at: 0 303 2795 3863 6165 6957
Size: 303 2492 1068 2302 792
Fragments arranged by size:
2492 2302 1068 792 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 759 791 5610 5642 6665 6697 6957
Size: 759 32 4819 32 1023 32 260
Fragments arranged by size:
4819 1023 759 260 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1311 4293 6703 6957
Size: 1311 2982 2410 254
Fragments arranged by size:
2982 2410 1311 254
XmnI GAAnn'nnTTC
Cuts at: 0 2372 4779 4959 5702 5947 6914 6957
Size: 2372 2407 180 743 245 967 43
Fragments arranged by size:
2407 2372 967 743 245 180 43
ZraI GAC'GTC
Cuts at: 0 3400 6957
Size: 3400 3557
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AhdI AlfI AlwNI
ApaI ApoI AseI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NsiI NspI PfoI PmlI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuII SacI SanDI
SapI SexAI SfcI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AleI AloI ApaLI
AscI AsiSI BaeI BbeI BglI BmtI BsiEI BsiWI
BsmBI BsrBI BssHII BssSI BstXI ClaI CspCI DrdI
EagI EciI FseI FspAI HaeII KasI KpnI MluI
NarI NheI NotI NruI PacI PasI PciI PflMI
PmeI PpiI PspXI PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI Tth111I XbaI XhoI XmaI