Restriction map for pF1KA0267
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# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaI        BsiEI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MunI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SgrAI       SmaI        SmlI        SpeI        
SphI        Sse8387I    SspBI       SwaI        XbaI        
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   42 enzymes) #
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AccII          10  CGCG                 CG^CG
AccIII       1604  TCCGGA               T^CCGGA
ApaI          284  GGGCCC               GGGCC^C
Asp700I      1865  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1994  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           56  CCCGAG               C^[CT]CG[GA]G
BalI         1394  TGGCCA               TGG^CCA
BcgI          118  GCAGGGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1623  TGATCA               T^GATCA
BcnI          282  CCGGG                CC^[GC]GG
BsaAI        1095  TACGTA               [CT]AC^GT[GA]
BsaBI        1762  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1604  TCCGGA               [AT]^CCGG[AT]
BseAI        1604  TCCGGA               T^CCGGA
BsmI          356  GCATTC               GAATGC(1/-1)
BsoBI          56  CCCGAG               C^[CT]CG[GA]G
BspEI        1604  TCCGGA               T^CCGGA
BssSI         718  CACGAG               CACGAG(-5/-1)
BstBI         518  TTCGAA               TT^CGAA
BstUI          10  CGCG                 CG^CG
BstXI         819  CCAGCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI         1623  TGATCA               T^GATCA
MaeII        1096  ACGT                 A^CGT
MamI         1762  GATTCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1394  TGGCCA               TGG^CCA
MroI         1604  TCCGGA               T^CCGGA
MscI         1394  TGGCCA               TGG^CCA
MvnI           10  CGCG                 CG^CG
NciI          282  CCGGG                CC^[GC]GG
NcoI          155  CCATGG               C^CATGG
NdeI         1802  CATATG               CA^TATG
NspV          518  TTCGAA               TT^CGAA
PflFI        1994  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI         1117  GAGTC                GAGTC(4/5)
PshAI         832  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         824  CAGCTG               CAG^CTG
SfuI          518  TTCGAA               TT^CGAA
SnaBI        1095  TACGTA               TAC^GTA
SspI         1282  AATATT               AAT^ATT
StuI          990  AGGCCT               AGG^CCT
Tth111I      1994  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1865  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI           40  GGCGTC               G[GA]^CG[CT]C
              103  GGCGTC              
AfaI         1016  GTAC                 GT^AC
             1614  GTAC                
AlwNI         683  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
              695  CAGTGACTG           
BanII          59  GAGCCC               G[GA]GC[CT]^C
              284  GGGCCC              
BfaI          149  CTAG                 C^TAG
              538  CTAG                
BsaHI          40  GGCGTC               G[GA]^CG[CT]C
              103  GGCGTC              
BsgI          295  CTGCAC               GTGCAG(16/14)
              403  GTGCAG              
BspMI         218  ACCTGC               ACCTGC(4/8)
              487  GCAGGT              
BstYI         878  GGATCT               [GA]^GATC[CT]
              901  GGATCT              
DdeI          391  CTGAG                C^T[ACGT]AG
             1121  CTCAG               
EaeI         1394  TGGCCA               [CT]^GGCC[GA]
             1832  CGGCCA              
EarI          268  CTCTTC               CTCTTC(1/4)
             1271  GAAGAG              
HaeII         211  AGCGCC               [GA]GCGC^[CT]
             1843  AGCGCC              
HgaI           41  GCGTC                GACGC(5/10)
              104  GCGTC               
Hin1I          40  GGCGTC               G[GA]^CG[CT]C
              103  GGCGTC              
Ksp632I       268  CTCTTC               CTCTTC(1/4)
             1271  GAAGAG              
MaeI          149  CTAG                 C^TAG
              538  CTAG                
MflI          878  GGATCT               [GA]^GATC[CT]
              901  GGATCT              
MseI         1226  TTAA                 T^TAA
             1307  TTAA                
PflMI         975  CCAGTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1911  CCATGAACTGG         
RsaI         1016  GTAC                 GT^AC
             1614  GTAC                
SapI         1271  GAAGAGC              GCTCTTC(1/4)
              267  GCTCTTC             
Tru9I        1226  TTAA                 T^TAA
             1307  TTAA                
Van91I        975  CCAGTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1911  CCATGAACTGG         
XhoII         878  GGATCT               [GA]^GATC[CT]
              901  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BanI        BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       Cac8I       
CfoI        Cfr10I      Cfr13I      DpnII       DsaI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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