Restriction map for pF1KA0247
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BseAI       BsgI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tsp509I     
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AlwI           84  GATCC                GGATC(4/5)
AlwNI         740  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          613  GGGCCC               GGGCC^C
Asp700I       438  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          27  GAGCAC               G[AT]GC[AT]^C
AspI          481  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          731  CTCGGG               C^[CT]CG[GA]G
BcgI          200  GCAGTGTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          170  CCCGG                CC^[GC]GG
BfaI           79  CTAG                 C^TAG
BsaWI         135  ACCGGA               [AT]^CCGG[AT]
BsiHKAI        27  GAGCAC               G[AT]GC[AT]^C
BsoBI         731  CTCGGG               C^[CT]CG[GA]G
BspLU11I      233  ACATGT               A^CATGT
BsrFI         784  GCCGGC               [GA]^CCGG[CT]
BssSI         403  CTCGTG               CACGAG(-5/-1)
BstAPI        642  GCAACAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        501  GGATG                GGATG(2/0)
CfoI          388  GCGC                 GCG^C
Cfr10I        784  GCCGGC               [GA]^CCGG[CT]
DpnII          84  GATC                 ^GATC
DraIII        559  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          782  TGGCCG               [CT]^GGCC[GA]
EarI          771  CTCTTC               CTCTTC(1/4)
FokI          501  GGATG                GGATG(9/13)
HaeII         387  GGCGCT               [GA]GCGC^[CT]
HhaI          388  GCGC                 GCG^C
HinP1I        388  GCGC                 G^CGC
Ksp632I       771  CTCTTC               CTCTTC(1/4)
MaeI           79  CTAG                 C^TAG
MboI           84  GATC                 ^GATC
MspA1I        646  CAGCTG               C[AC]G^C[GT]G
NaeI          784  GCCGGC               GCC^GGC
NciI          170  CCCGG                CC^[GC]GG
NdeII          84  GATC                 ^GATC
NgoMIV        784  GCCGGC               G^CCGGC
NspI          233  ACATGT               [GA]CATG^[CT]
PflFI         481  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         142  CCAGAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          506  GAGTC                GAGTC(4/5)
PpuMI         344  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         646  CAGCTG               CAG^CTG
Sau3AI         84  GATC                 ^GATC
SexAI         482  ACCAGGT              A^CC[AT]GGT
SfaNI         756  GCATC                GCATC(5/9)
TaqI          209  TCGA                 T^CGA
TfiI          826  GATTC                G^A[AT]TC
Tth111I       481  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       209  TCGA                 T^CGA
Van91I        142  CCAGAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          438  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI           76  CCGC                 CCGC(-3/-1)
              798  CCGC                
AfaI          586  GTAC                 GT^AC
              876  GTAC                
AflIII        233  ACATGT               A^C[GA][CT]GT
              262  ACGTGT              
BanII         613  GGGCCC               G[GA]GC[CT]^C
              726  GGGCTC              
BsaI          182  GAGACC               GGTCTC(1/5)
              486  GGTCTC              
BsmBI         307  CGTCTC               CGTCTC(1/5)
              104  GAGACG              
BspMI         570  ACCTGC               ACCTGC(4/8)
              676  GCAGGT              
BstXI         377  CCAGCTCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              491  CCATCATGGTGG        
HinfI         506  GAGTC                G^A[ACGT]TC
              826  GATTC               
MaeII         262  ACGT                 A^CGT
              865  ACGT                
MboII         685  GAAGA                GAAGA(8/7)
              772  TCTTC               
MslI           30  CACCTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              492  CATCATGGTG          
NcoI            6  CCATGG               C^CATGG
              290  CCATGG              
RsaI          586  GTAC                 GT^AC
              876  GTAC                
XcmI          330  CCACACATCACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              465  CCAGGGGGTATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AluI        AvaII       BbvI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DraII       DsaI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HpaII       ItaI        MaeIII      MnlI        MspI        
MvaI        MwoI        NlaIII      NlaIV       PstI        
Sau96I      ScrFI       SfcI        StyI        TseI        
Tsp45I      TspRI       
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