Restriction map for pF1KA0247
 (Linear) (Six-base) MAPSORT of: KIAA0247.seq  Check: 6165  from: 1  to: 909



KIAA0247.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     579     667     909 
   Size:        579      88     242

  Fragments arranged by size: 

                 579     242      88
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     457     909 
   Size:        245     212     452

  Fragments arranged by size: 

                 452     245     212
 
AflIII A'CryG_T

Cuts at:      0     233     262     909 
   Size:        233      29     647

  Fragments arranged by size: 

                 647     233      29
 
AleI CACnn'nnGTG

Cuts at:      0      34     909 
   Size:         34     875
 
AlwNI CAG_nnn'CTG

Cuts at:      0     745     909 
   Size:        745     164
 
ApaI G_GGCC'C

Cuts at:      0     617     909 
   Size:        617     292
 
AvaI C'yCGr_G

Cuts at:      0     731     909 
   Size:        731     178
 
BanII G_rGCy'C

Cuts at:      0     617     730     909 
   Size:        617     113     179

  Fragments arranged by size: 

                 617     179     113
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     189     223     909 
   Size:        189      34     686

  Fragments arranged by size: 

                 686     189      34
 
Bme1580I G_kGCm'C

Cuts at:      0     127     617     909 
   Size:        127     490     292

  Fragments arranged by size: 

                 490     292     127
 
BmgBI CAC'GTC

Cuts at:      0     263     866     909 
   Size:        263     603      43

  Fragments arranged by size: 

                 603     263      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     611     909 
   Size:        611     298
 
Bpu10I CC'TnA_GC

Cuts at:      0     789     909 
   Size:        789     120
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     176     492     909 
   Size:        176     316     417

  Fragments arranged by size: 

                 417     316     176
 
BsaWI w'CCGG_w

Cuts at:      0     135     909 
   Size:        135     774
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     472     497     502     527     909 
   Size:        472      25       5      25     382

  Fragments arranged by size: 

                 472     382      25      25       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     388     391     547     639     660     886     909 
   Size:        388       3     156      92      21     226      23

  Fragments arranged by size: 

                 388     226     156      92      23      21       3
 
BsiHKAI G_wGCw'C

Cuts at:      0      31     909 
   Size:         31     878
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      98     313     909 
   Size:         98     215     596

  Fragments arranged by size: 

                 596     215      98
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     127     617     730     909 
   Size:         31      96     490     113     179

  Fragments arranged by size: 

                 490     179     113      96      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     579     667     909 
   Size:        579      88     242

  Fragments arranged by size: 

                 579     242      88
 
BsrFI r'CCGG_y

Cuts at:      0     784     909 
   Size:        784     125
 
BssSI C'ACGA_G

Cuts at:      0     403     909 
   Size:        403     506
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     648     909 
   Size:        648     261
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     384     498     909 
   Size:        384     114     411

  Fragments arranged by size: 

                 411     384     114
 
BtgI C'CryG_G

Cuts at:      0       6      44      53     290     383     909 
   Size:          6      38       9     237      93     526

  Fragments arranged by size: 

                 526     237      93      38       9       6
 
BtsI GCAGTG_nn'

Cuts at:      0     207     909 
   Size:        207     702
 
DraIII CAC_nnn'GTG

Cuts at:      0     564     909 
   Size:        564     345
 
EaeI y'GGCC_r

Cuts at:      0     782     909 
   Size:        782     127
 
EarI CTCTTCn'nnn_

Cuts at:      0     777     909 
   Size:        777     132
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     457     909 
   Size:        245     212     452

  Fragments arranged by size: 

                 452     245     212
 
EcoO109I rG'GnC_Cy

Cuts at:      0     345     662     693     909 
   Size:        345     317      31     216

  Fragments arranged by size: 

                 345     317     216      31
 
HaeII r_GCGC'y

Cuts at:      0     391     909 
   Size:        391     518
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     472     504     741     773     909 
   Size:        472      32     237      32     136

  Fragments arranged by size: 

                 472     237     136      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      34     496     909 
   Size:         34     462     413

  Fragments arranged by size: 

                 462     413      34
 
MspA1I CmG'CkG

Cuts at:      0     648     909 
   Size:        648     261
 
NaeI GCC'GGC

Cuts at:      0     786     909 
   Size:        786     123
 
NcoI C'CATG_G

Cuts at:      0       6     290     909 
   Size:          6     284     619

  Fragments arranged by size: 

                 619     284       6
 
NgoMIV G'CCGG_C

Cuts at:      0     784     909 
   Size:        784     125
 
NspI r_CATG'y

Cuts at:      0     237     909 
   Size:        237     672
 
PciI A'CATG_T

Cuts at:      0     233     909 
   Size:        233     676
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     148     909 
   Size:        148     761
 
PpuMI rG'GwC_Cy

Cuts at:      0     345     909 
   Size:        345     564
 
PspOMI G'GGCC_C

Cuts at:      0     613     909 
   Size:        613     296
 
PstI C_TGCA'G

Cuts at:      0     181     428     678     825     909 
   Size:        181     247     250     147      84

  Fragments arranged by size: 

                 250     247     181     147      84
 
PvuII CAG'CTG

Cuts at:      0     648     909 
   Size:        648     261
 
SanDI GG'GwC_CC

Cuts at:      0     345     909 
   Size:        345     564
 
SexAI A'CCwGG_T

Cuts at:      0     482     909 
   Size:        482     427
 
SfcI C'TryA_G

Cuts at:      0     177     359     424     674     821     909 
   Size:        177     182      65     250     147      88

  Fragments arranged by size: 

                 250     182     177     147      88      65
 
StyI C'CwwG_G

Cuts at:      0       6     290     634     657     909 
   Size:          6     284     344      23     252

  Fragments arranged by size: 

                 344     284     252      23       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     343     768     909 
   Size:        343     425     141

  Fragments arranged by size: 

                 425     343     141
 
TatI w'GTAC_w

Cuts at:      0     585     875     909 
   Size:        585     290      34

  Fragments arranged by size: 

                 585     290      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      50      82     329     361     909 
   Size:         50      32     247      32     548

  Fragments arranged by size: 

                 548     247      50      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     484     909 
   Size:        484     425
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     337     472     909 
   Size:        337     135     437

  Fragments arranged by size: 

                 437     337     135
 
XmnI GAAnn'nnTTC

Cuts at:      0     442     909 
   Size:        442     467
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApaI     AvaI    BanII
     BcgI Bme1580I    BmgBI     BmrI   Bpu10I     BsaI    BsaWI    BsaXI
    BseRI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI    BssSI   BstAPI
    BstXI     BtgI     BtsI   DraIII     EaeI     EarI  Eco57MI EcoO109I
    HaeII    Hin4I     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PciI    PflMI    PpuMI   PspOMI     PstI    PvuII    SanDI    SexAI
     SfcI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmtI     BplI     BpmI    BpuEI
    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     XbaI     XhoI     XmaI     ZraI
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