Restriction map for pF1KA0231
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

Acc65I      AccIII      AflII       AflIII      AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BanI        BbeI        BbrPI       BcgI        
BciVI       BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
FseI        FspI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SwaI        Van91I      XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII          73  GACGTC               GACGT^C
AccI         1429  GTAGAC               GT^[AC][GT]AC
AccII         499  CGCG                 CG^CG
AclI         2381  AACGTT               AA^CGTT
AcyI           73  GACGTC               G[GA]^CG[CT]C
Aor51HI       899  AGCGCT               AGC^GCT
AspI         1300  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          445  CTCGAG               C^[CT]CG[GA]G
BalI          458  TGGCCA               TGG^CCA
BamHI        1652  GGATCC               G^GATCC
BbvCI        1011  GCTGAGG              CCTCAGC(-5/-2)
BcnI         1649  CCCGG                CC^[GC]GG
BglI          115  GCCGTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI          73  GACGTC               G[GA]^CG[CT]C
BsmAI        1375  GTCTC                GTCTC(1/5)
BsoBI         445  CTCGAG               C^[CT]CG[GA]G
BsrBI         262  CCGCTC               CCGCTC(-3/-3)
BstUI         499  CGCG                 CG^CG
BstXI         668  CCAGTGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         1464  TTTAAA               TTT^AAA
Eco47III      899  AGCGCT               AGC^GCT
EcoNI        1161  CCTATCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         275  GAATTC               G^AATTC
EcoT22I       736  ATGCAT               ATGCA^T
FauI          497  CCCGC                CCCGC(4/6)
HaeII         899  AGCGCT               [GA]GCGC^[CT]
HgaI          141  GACGC                GACGC(5/10)
Hin1I          73  GACGTC               G[GA]^CG[CT]C
HindIII      1237  AAGCTT               A^AGCTT
HpaI         1314  GTTAAC               GTT^AAC
MluNI         458  TGGCCA               TGG^CCA
MscI          458  TGGCCA               TGG^CCA
MvnI          499  CGCG                 CG^CG
NciI         1649  CCCGG                CC^[GC]GG
NsiI          736  ATGCAT               ATGCA^T
PaeR7I        445  CTCGAG               C^TCGAG
PflFI        1300  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          640  GAGTC                GAGTC(4/5)
Psp1406I     2381  AACGTT               AA^CGTT
SapI         1635  GAAGAGC              GCTCTTC(1/4)
SfiI          114  GGCCGTGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          734  GCATGC               GCATG^C
StuI          535  AGGCCT               AGG^CCT
Tth111I      1300  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1452  TCTAGA               T^CTAGA
XhoI          445  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI          575  CCTCAGG              CC^T[ACGT]AGG
             1842  CCTAAGG             
Asp700I       723  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2281  GAAACACTTC          
BanII         128  GAGCTC               G[GA]GC[CT]^C
             2260  GAGCTC              
BstYI        1652  GGATCC               [GA]^GATC[CT]
             2052  GGATCT              
Bsu36I        575  CCTCAGG              CC^T[ACGT]AGG
             1842  CCTAAGG             
CfoI          500  GCGC                 GCG^C
              900  GCGC                
DraII         938  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1621  AGGACCC             
DsaI           64  CCATGG               C^C[GA][CT]GG
              487  CCATGG              
Eco81I        575  CCTCAGG              CC^T[ACGT]AGG
             1842  CCTAAGG             
EcoO109I      938  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1621  AGGACCC             
HhaI          500  GCGC                 GCG^C
              900  GCGC                
HinP1I        500  GCGC                 G^CGC
              900  GCGC                
HincII       1314  GTTAAC               GT[CT]^[GA]AC
             1387  GTCAAC              
HindII       1314  GTTAAC               GT[CT]^[GA]AC
             1387  GTCAAC              
MflI         1652  GGATCC               [GA]^GATC[CT]
             2052  GGATCT              
MslI          735  CATGCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2227  CATGTGGGTG          
NcoI           64  CCATGG               C^CATGG
              487  CCATGG              
NlaIV        1622  GGACCC               GG[ACGT]^[ACGT]CC
             1652  GGATCC              
NspI          734  GCATGC               [GA]CATG^[CT]
              738  GCATGT              
PpuMI         938  AGGTCCT              [GA]G^G[AT]CC[CT]
             1621  AGGACCC             
SacI          128  GAGCTC               GAGCT^C
             2260  GAGCTC              
XhoII        1652  GGATCC               [GA]^GATC[CT]
             2052  GGATCT              
XmnI          723  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2281  GAAACACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BbsI        
BbvI        BclI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CelII       Cfr13I      DdeI        
DpnII       EaeI        EarI        EcoRII      EcoT14I     
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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