Restriction map for pF1KA0231
 (Linear) (Six-base) MAPSORT of: KIAA0231.seq  Check: 8703  from: 1  to: 2409



KIAA0231.orf

 With 182 enzymes: SgfI * 

                        November 13, 2006 11:20  ..

AatII G_ACGT'C

Cuts at:      0      77    2409 
   Size:         77    2332
 
AccI GT'mk_AC

Cuts at:      0    1430    2409 
   Size:       1430     979
 
AclI AA'CG_TT

Cuts at:      0    2382    2409 
   Size:       2382      27
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1045    1879    2409 
   Size:       1045     834     530

  Fragments arranged by size: 

                1045     834     530
 
AfeI AGC'GCT

Cuts at:      0     901    2409 
   Size:        901    1508
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2135    2194    2409 
   Size:       2135      59     215

  Fragments arranged by size: 

                2135     215      59
 
AlwNI CAG_nnn'CTG

Cuts at:      0     520    1695    2100    2409 
   Size:        520    1175     405     309

  Fragments arranged by size: 

                1175     520     405     309
 
ApoI r'AATT_y

Cuts at:      0     181     275    1461    1483    2214    2409 
   Size:        181      94    1186      22     731     195

  Fragments arranged by size: 

                1186     731     195     181      94      22
 
AvaI C'yCGr_G

Cuts at:      0     445    2409 
   Size:        445    1964
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     414     447    2409 
   Size:        414      33    1962

  Fragments arranged by size: 

                1962     414      33
 
BamHI G'GATC_C

Cuts at:      0    1652    2409 
   Size:       1652     757
 
BanII G_rGCy'C

Cuts at:      0     132    2264    2409 
   Size:        132    2132     145

  Fragments arranged by size: 

                2132     145     132
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      69     157    1285    2409 
   Size:         69      88    1128    1124

  Fragments arranged by size: 

                1128    1124      88      69
 
BbvCI CC'TCA_GC

Cuts at:      0    1012    2409 
   Size:       1012    1397
 
BclI T'GATC_A

Cuts at:      0      98    1119    1775    2409 
   Size:         98    1021     656     634

  Fragments arranged by size: 

                1021     656     634      98
 
BfrBI ATG'CAT

Cuts at:      0     738    2409 
   Size:        738    1671
 
BglI GCCn_nnn'nGGC

Cuts at:      0     121    2409 
   Size:        121    2288
 
BlpI GC'TnA_GC

Cuts at:      0      27     521    1333    2409 
   Size:         27     494     812    1076

  Fragments arranged by size: 

                1076     812     494      27
 
BmgBI CAC'GTC

Cuts at:      0    2393    2409 
   Size:       2393      16
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      33     422    2409 
   Size:         33     389    1987

  Fragments arranged by size: 

                1987     389      33
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1998    2409 
   Size:       1998     411
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1360    2278    2409 
   Size:        118    1242     918     131

  Fragments arranged by size: 

                1242     918     131     118
 
Bpu10I CC'TnA_GC

Cuts at:      0    1012    2409 
   Size:       1012    1397
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1378    1510    1519    2409 
   Size:       1378     132       9     890

  Fragments arranged by size: 

                1378     890     132       9
 
BsaHI Gr'CG_yC

Cuts at:      0      74    2409 
   Size:         74    2335
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2274    2304    2409 
   Size:       2274      30     105

  Fragments arranged by size: 

                2274     105      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1029    1471    2356    2409 
   Size:       1029     442     885      53

  Fragments arranged by size: 

                1029     885     442      53
 
BseYI C'CCAG_C

Cuts at:      0    1511    2409 
   Size:       1511     898
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     898    1424    1931    2409 
   Size:        898     526     507     478

  Fragments arranged by size: 

                 898     526     507     478
 
BsiHKAI G_wGCw'C

Cuts at:      0     132     377     812    2264    2409 
   Size:        132     245     435    1452     145

  Fragments arranged by size: 

                1452     435     245     145     132
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     377     812    2264    2409 
   Size:        132     245     435    1452     145

  Fragments arranged by size: 

                1452     435     245     145     132
 
BsrBI CCG'CTC

Cuts at:      0     264    2409 
   Size:        264    2145
 
BsrDI GCAATG_nn'

Cuts at:      0     613    1684    1931    2409 
   Size:        613    1071     247     478

  Fragments arranged by size: 

                1071     613     478     247
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     675    2409 
   Size:        675    1734
 
BstYI r'GATC_y

Cuts at:      0    1652    2052    2409 
   Size:       1652     400     357

  Fragments arranged by size: 

                1652     400     357
 
Bsu36I CC'TnA_GG

Cuts at:      0     576    1843    2409 
   Size:        576    1267     566

  Fragments arranged by size: 

                1267     576     566
 
BtgI C'CryG_G

Cuts at:      0      64     487    2409 
   Size:         64     423    1922

  Fragments arranged by size: 

                1922     423      64
 
BtsI GCAGTG_nn'

Cuts at:      0    2188    2409 
   Size:       2188     221
 
DraI TTT'AAA

Cuts at:      0    1466    2409 
   Size:       1466     943
 
EaeI y'GGCC_r

Cuts at:      0     113     122     458    2409 
   Size:        113       9     336    1951

  Fragments arranged by size: 

                1951     336     113       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     993    1630    1865    2366    2409 
   Size:        993     637     235     501      43

  Fragments arranged by size: 

                 993     637     501     235      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1465    2409 
   Size:       1465     944
 
EcoICRI GAG'CTC

Cuts at:      0     130    2262    2409 
   Size:        130    2132     147

  Fragments arranged by size: 

                2132     147     130
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1045    1360    1879    2278    2409 
   Size:        118     927     315     519     399     131

  Fragments arranged by size: 

                 927     519     399     315     131     118
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1165    2409 
   Size:       1165    1244
 
EcoO109I rG'GnC_Cy

Cuts at:      0     939    1622    2409 
   Size:        939     683     787

  Fragments arranged by size: 

                 939     787     683
 
EcoRI G'AATT_C

Cuts at:      0     275    2409 
   Size:        275    2134
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     680    1110    2409 
   Size:        680     430    1299

  Fragments arranged by size: 

                1299     680     430
 
HaeII r_GCGC'y

Cuts at:      0     903    2409 
   Size:        903    1506
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57    1740    1772    2409 
   Size:         25      32    1683      32     637

  Fragments arranged by size: 

                1683     637      32      32      25
 
HincII GTy'rAC

Cuts at:      0    1316    1389    2409 
   Size:       1316      73    1020

  Fragments arranged by size: 

                1316    1020      73
 
HindIII A'AGCT_T

Cuts at:      0    1237    2409 
   Size:       1237    1172
 
HpaI GTT'AAC

Cuts at:      0    1316    2409 
   Size:       1316    1093
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1571    2409 
   Size:       1571     838
 
MscI TGG'CCA

Cuts at:      0     460    2409 
   Size:        460    1949
 
MslI CAynn'nnrTG

Cuts at:      0     739    2231    2409 
   Size:        739    1492     178

  Fragments arranged by size: 

                1492     739     178
 
MspA1I CmG'CkG

Cuts at:      0     138     589     646    1383    1781    2409 
   Size:        138     451      57     737     398     628

  Fragments arranged by size: 

                 737     628     451     398     138      57
 
NcoI C'CATG_G

Cuts at:      0      64     487    2409 
   Size:         64     423    1922

  Fragments arranged by size: 

                1922     423      64
 
NsiI A_TGCA'T

Cuts at:      0     740    2409 
   Size:        740    1669
 
NspI r_CATG'y

Cuts at:      0     738     742    2409 
   Size:        738       4    1667

  Fragments arranged by size: 

                1667     738       4
 
PfoI T'CCnGG_A

Cuts at:      0     674    1648    2409 
   Size:        674     974     761

  Fragments arranged by size: 

                 974     761     674
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255    2409 
   Size:        223      32    2154

  Fragments arranged by size: 

                2154     223      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     939    1622    2409 
   Size:        939     683     787

  Fragments arranged by size: 

                 939     787     683
 
PspXI vC'TCGA_Gb

Cuts at:      0     445    2409 
   Size:        445    1964
 
PstI C_TGCA'G

Cuts at:      0     406    1374    2183    2409 
   Size:        406     968     809     226

  Fragments arranged by size: 

                 968     809     406     226
 
PvuII CAG'CTG

Cuts at:      0     138     589     646    1383    1781    2409 
   Size:        138     451      57     737     398     628

  Fragments arranged by size: 

                 737     628     451     398     138      57
 
SacI G_AGCT'C

Cuts at:      0     132    2264    2409 
   Size:        132    2132     145

  Fragments arranged by size: 

                2132     145     132
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1630    2409 
   Size:       1630     779
 
ScaI AGT'ACT

Cuts at:      0     304    1581    2116    2409 
   Size:        304    1277     535     293

  Fragments arranged by size: 

                1277     535     304     293
 
SfcI C'TryA_G

Cuts at:      0     402     993    1065    1370    2179    2409 
   Size:        402     591      72     305     809     230

  Fragments arranged by size: 

                 809     591     402     305     230      72
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     121    2409 
   Size:        121    2288
 
SmlI C'TyrA_G

Cuts at:      0     445    1393    1525    1534    2409 
   Size:        445     948     132       9     875

  Fragments arranged by size: 

                 948     875     445     132       9
 
SphI G_CATG'C

Cuts at:      0     738    2409 
   Size:        738    1671
 
SspI AAT'ATT

Cuts at:      0    1033    2001    2139    2409 
   Size:       1033     968     138     270

  Fragments arranged by size: 

                1033     968     270     138
 
StuI AGG'CCT

Cuts at:      0     537    2409 
   Size:        537    1872
 
StyI C'CwwG_G

Cuts at:      0      64     205     487     920    2409 
   Size:         64     141     282     433    1489

  Fragments arranged by size: 

                1489     433     282     141      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2221    2409 
   Size:       2221     188
 
TatI w'GTAC_w

Cuts at:      0     302    1579    1628    2114    2409 
   Size:        302    1277      49     486     295

  Fragments arranged by size: 

                1277     486     302     295      49
 
Tth111I GACn'n_nGTC

Cuts at:      0    1303    2409 
   Size:       1303    1106
 
XbaI T'CTAG_A

Cuts at:      0    1452    2409 
   Size:       1452     957
 
XhoI C'TCGA_G

Cuts at:      0     445    2409 
   Size:        445    1964
 
XmnI GAAnn'nnTTC

Cuts at:      0     727    2285    2409 
   Size:        727    1558     124

  Fragments arranged by size: 

                1558     727     124
 
ZraI GAC'GTC

Cuts at:      0      75    2409 
   Size:         75    2334
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI     AfeI     AlfI    AlwNI     ApoI
     AvaI     BaeI    BamHI    BanII     BbsI    BbvCI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrBI
    BsrDI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI
   MspA1I     NcoI     NsiI     NspI     PfoI     PpiI    PpuMI    PspXI
     PstI    PvuII     SacI     SapI     ScaI     SfcI     SfiI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I    AflII   AflIII     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BanI     BbeI
     BcgI    BciVI    BglII Bme1580I     BmtI     BsaI    BsaAI    BsaBI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI    EcoRV     FseI
     FspI    FspAI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     TstI     XcmI
     XmaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com