Restriction map for pF1KA0203
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AspI        AvaI        
AvrII       BanI        BbeI        BbrPI       BbvCI       
BcnI        BlnI        BsaAI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsoBI       
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BssHII      BstAPI      BstEII      BstPI       BstUI       
Cfr10I      ClaI        CpoI        DraII       DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO109I    EcoO65I     EcoRI       EcoRV       
FauI        FseI        HaeII       HapII       HgaI        
Hin1I       HpaII       KasI        KpnI        KspI        
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    StuI        
Tth111I     XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AflII          82  CTTAAG               C^TTAAG
Alw44I       4238  GTGCAC               G^TGCAC
AocI         1788  CCTCAGG              CC^T[ACGT]AGG
ApaLI        4238  GTGCAC               G^TGCAC
AseI         3073  ATTAAT               AT^TAAT
AsnI         3073  ATTAAT               AT^TAAT
AvaII        1116  GGACC                G^G[AT]CC
AviII        4175  TGCGCA               TGC^GCA
BamHI        2031  GGATCC               G^GATCC
BcgI         3951  GCAAAATGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1386  TGATCA               T^GATCA
BfrI           82  CTTAAG               C^TTAAG
BglI         4690  GCCAAAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1899  AGATCT               A^GATCT
BsaI         3259  GAGACC               GGTCTC(1/5)
Bst1107I     2432  GTATAC               GTA^TAC
BstBI        3959  TTCGAA               TT^CGAA
BstZ17I      2432  GTATAC               GTA^TAC
Bsu36I       1788  CCTCAGG              CC^T[ACGT]AGG
CfoI         4176  GCGC                 GCG^C
DraIII        124  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1788  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2703  CCTTGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1386  TGATCA               T^GATCA
FspI         4175  TGCGCA               TGC^GCA
HhaI         4176  GCGC                 GCG^C
HinP1I       4176  GCGC                 G^CGC
MaeII        2095  ACGT                 A^CGT
NheI         1129  GCTAGC               G^CTAGC
NspV         3959  TTCGAA               TT^CGAA
PflMI         126  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4436  GACTGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3073  ATTAAT               AT^TAAT
SacI         4124  GAGCTC               GAGCT^C
SapI         3919  GAAGAGC              GCTCTTC(1/4)
ScaI          850  AGTACT               AGT^ACT
SexAI         125  ACCAGGT              A^CC[AT]GGT
SfuI         3959  TTCGAA               TT^CGAA
SphI           87  GCATGC               GCATG^C
SspI         4342  AATATT               AAT^ATT
SwaI         3744  ATTTAAAT             ATTT^AAAT
Van91I        126  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflIII        789  ACATGT               A^C[GA][CT]GT
             1344  ACATGT              
BalI          461  TGGCCA               TGG^CCA
              596  TGGCCA              
BanII        3245  GAGCCC               G[GA]GC[CT]^C
             4124  GAGCTC              
BciVI         198  GGATAC               GTATCC(6/5)
             2334  GGATAC              
BlpI         1490  GCTTAGC              GC^T[ACGT]AGC
             3362  GCTTAGC             
BpmI         2072  CTCCAG               CTGGAG(16/14)
              469  CTGGAG              
Bpu1102I     1490  GCTTAGC              GC^T[ACGT]AGC
             3362  GCTTAGC             
BsmBI        2237  CGTCTC               CGTCTC(1/5)
              697  GAGACG              
BspLU11I      789  ACATGT               A^CATGT
             1344  ACATGT              
BspMI         252  ACCTGC               ACCTGC(4/8)
             4605  ACCTGC              
BsrDI        4394  CATTGC               GCAATG(2/0)
             4309  GCAATG              
BssSI        2861  CACGAG               CACGAG(-5/-1)
             3165  CACGAG              
BstXI         710  CCACTGAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              776  CCAAGTCAGTGG        
CelII        1490  GCTTAGC              GC^T[ACGT]AGC
             3362  GCTTAGC             
Cfr13I       1116  GGACC                G^G[ACGT]CC
             1673  GGCCC               
EcoT22I       153  ATGCAT               ATGCA^T
             1214  ATGCAT              
HincII         22  GTTAAC               GT[CT]^[GA]AC
              762  GTTAAC              
HindII         22  GTTAAC               GT[CT]^[GA]AC
              762  GTTAAC              
HpaI           22  GTTAAC               GTT^AAC
              762  GTTAAC              
MfeI         3389  CAATTG               C^AATTG
             4399  CAATTG              
MluNI         461  TGGCCA               TGG^CCA
              596  TGGCCA              
MscI          461  TGGCCA               TGG^CCA
              596  TGGCCA              
MslI         1825  CACCAGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2507  CATTAAAATG          
MspA1I       4271  CCGCTG               C[AC]G^C[GT]G
             4369  CAGCGG              
MunI         3389  CAATTG               C^AATTG
             4399  CAATTG              
NdeI         4202  CATATG               CA^TATG
             4348  CATATG              
NsiI          153  ATGCAT               ATGCA^T
             1214  ATGCAT              
Sau96I       1116  GGACC                G^G[ACGT]CC
             1673  GGCCC               
XbaI         3906  TCTAGA               T^CTAGA
             4639  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
BbsI        BbvI        BfaI        BmyI        BpuAI       
BsaBI       BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
Bsp1407I    BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       DdeI        DpnII       
DraI        EaeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HindIII     HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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