Restriction map for
pF1KA0164
(Linear) (Six-base) MAPSORT of: KIAA0164.seq Check: 1991 from: 1 to: 2760
KIAA0164.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 1573 2760
Size: 1573 1187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 1134 1563 1655 1787 1937 2246 2760
Size: 527 607 429 92 132 150 309 514
Fragments arranged by size:
607 527 514 429 309 150 132 92
ApoI r'AATT_y
Cuts at: 0 601 1449 1750 2326 2760
Size: 601 848 301 576 434
Fragments arranged by size:
848 601 576 434 301
AvaI C'yCGr_G
Cuts at: 0 2288 2760
Size: 2288 472
BamHI G'GATC_C
Cuts at: 0 2505 2760
Size: 2505 255
BanI G'GyrC_C
Cuts at: 0 1653 2760
Size: 1653 1107
BanII G_rGCy'C
Cuts at: 0 1945 2140 2760
Size: 1945 195 620
Fragments arranged by size:
1945 620 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 1448 2760
Size: 1448 1312
BglII A'GATC_T
Cuts at: 0 76 382 400 2760
Size: 76 306 18 2360
Fragments arranged by size:
2360 306 76 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 1007 2276 2760
Size: 1007 1269 484
Fragments arranged by size:
1269 1007 484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1640 2760
Size: 1640 1120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2124 2760
Size: 2124 636
Bpu10I CC'TnA_GC
Cuts at: 0 1490 2760
Size: 1490 1270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1291 2760
Size: 1291 1469
BsaI GGTCTCn'nnnn_
Cuts at: 0 116 140 416 485 2478 2701 2760
Size: 116 24 276 69 1993 223 59
Fragments arranged by size:
1993 276 223 116 69 59 24
BsaBI GATnn'nnATC
Cuts at: 0 69 75 2253 2760
Size: 69 6 2178 507
Fragments arranged by size:
2178 507 69 6
BsaWI w'CCGG_w
Cuts at: 0 625 1283 2760
Size: 625 658 1477
Fragments arranged by size:
1477 658 625
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 263 266 1222 1309 2441 2760
Size: 263 3 956 87 1132 319
Fragments arranged by size:
1132 956 319 263 87 3
BseYI C'CCAG_C
Cuts at: 0 2437 2760
Size: 2437 323
BsmI GAATG_Cn'
Cuts at: 0 780 930 2760
Size: 780 150 1830
Fragments arranged by size:
1830 780 150
BsmBI CGTCTCn'nnnn_
Cuts at: 0 195 2760
Size: 195 2565
Bsp1286I G_dGCh'C
Cuts at: 0 1945 2140 2760
Size: 1945 195 620
Fragments arranged by size:
1945 620 195
BspHI T'CATG_A
Cuts at: 0 2664 2760
Size: 2664 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1485 1692 2760
Size: 1485 207 1068
Fragments arranged by size:
1485 1068 207
BsrBI CCG'CTC
Cuts at: 0 1890 2760
Size: 1890 870
BsrDI GCAATG_nn'
Cuts at: 0 1880 2760
Size: 1880 880
BsrFI r'CCGG_y
Cuts at: 0 389 2760
Size: 389 2371
BssSI C'ACGA_G
Cuts at: 0 1562 2384 2760
Size: 1562 822 376
Fragments arranged by size:
1562 822 376
BstBI TT'CG_AA
Cuts at: 0 2400 2760
Size: 2400 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2683 2760
Size: 2683 77
BstYI r'GATC_y
Cuts at: 0 76 352 370 382 400 838 2505 2760
Size: 76 276 18 12 18 438 1667 255
Fragments arranged by size:
1667 438 276 255 76 18 18 12
Bsu36I CC'TnA_GG
Cuts at: 0 1984 2760
Size: 1984 776
BtgI C'CryG_G
Cuts at: 0 2711 2760
Size: 2711 49
ClaI AT'CG_AT
Cuts at: 0 596 2760
Size: 596 2164
DraI TTT'AAA
Cuts at: 0 2025 2331 2760
Size: 2025 306 429
Fragments arranged by size:
2025 429 306
DraIII CAC_nnn'GTG
Cuts at: 0 1827 2760
Size: 1827 933
EarI CTCTTCn'nnn_
Cuts at: 0 432 518 1022 2006 2265 2412 2489 2583
Size: 432 86 504 984 259 147 77 94
Cuts at: 2583 2699 2760
Size: 116 61
Fragments arranged by size:
984 504 432 259 147 116 94 86
77 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 1134 1563 1655 1787 1937 2124 2246
Size: 527 607 429 92 132 150 187 122
Cuts at: 2246 2760
Size: 514
Fragments arranged by size:
607 527 514 429 187 150 132 122
92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 317 2760
Size: 317 2443
EcoRI G'AATT_C
Cuts at: 0 1750 2760
Size: 1750 1010
EcoRV GAT'ATC
Cuts at: 0 262 2760
Size: 262 2498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1593 2553 2556 2760
Size: 1593 960 3 204
Fragments arranged by size:
1593 960 204 3
HaeII r_GCGC'y
Cuts at: 0 2625 2760
Size: 2625 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 1078 1110 1147 1179 2760
Size: 1015 32 31 32 37 32 1581
Fragments arranged by size:
1581 1015 37 32 32 32 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 2760
Size: 810 1950
MslI CAynn'nnrTG
Cuts at: 0 269 2760
Size: 269 2491
MspA1I CmG'CkG
Cuts at: 0 1870 2760
Size: 1870 890
NcoI C'CATG_G
Cuts at: 0 2711 2760
Size: 2711 49
NruI TCG'CGA
Cuts at: 0 176 2760
Size: 176 2584
PfoI T'CCnGG_A
Cuts at: 0 334 2760
Size: 334 2426
PshAI GACnn'nnGTC
Cuts at: 0 477 2760
Size: 477 2283
PsiI TTA'TAA
Cuts at: 0 1376 2760
Size: 1376 1384
PvuII CAG'CTG
Cuts at: 0 1870 2760
Size: 1870 890
SapI GCTCTTCn'nnn_
Cuts at: 0 2412 2760
Size: 2412 348
ScaI AGT'ACT
Cuts at: 0 1929 2536 2760
Size: 1929 607 224
Fragments arranged by size:
1929 607 224
SmlI C'TyrA_G
Cuts at: 0 1306 2288 2760
Size: 1306 982 472
Fragments arranged by size:
1306 982 472
StuI AGG'CCT
Cuts at: 0 1410 2760
Size: 1410 1350
StyI C'CwwG_G
Cuts at: 0 1832 2132 2676 2711 2760
Size: 1832 300 544 35 49
Fragments arranged by size:
1832 544 300 49 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2502 2760
Size: 2502 258
TatI w'GTAC_w
Cuts at: 0 745 1310 1717 1927 2534 2760
Size: 745 565 407 210 607 226
Fragments arranged by size:
745 607 565 407 226 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1385 1417 1813 1845 2760
Size: 1385 32 396 32 915
Fragments arranged by size:
1385 915 396 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 320 2760
Size: 320 2440
XbaI T'CTAG_A
Cuts at: 0 16 43 85 160 1033 1140 2760
Size: 16 27 42 75 873 107 1620
Fragments arranged by size:
1620 873 107 75 42 27 16
XhoI C'TCGA_G
Cuts at: 0 2288 2760
Size: 2288 472
XmnI GAAnn'nnTTC
Cuts at: 0 126 2536 2760
Size: 126 2410 224
Fragments arranged by size:
2410 224 126
Enzymes that do cut:
AccI AcuI ApoI AvaI BamHI BanI BanII BbsI
BglII BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaWI BseRI BseYI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI Bsu36I
BtgI ClaI DraI DraIII EarI Eco57MI EcoNI EcoRI
EcoRV FalI HaeII Hin4I MmeI MslI MspA1I NcoI
NruI PfoI PshAI PsiI PvuII SapI ScaI SmlI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BsaAI
BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI BspEI BsrGI
BssHII BstAPI BstEII BstZ17I BtgZI BtsI CspCI DrdI
EaeI EagI EciI EcoICRI EcoO109I FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XcmI XmaI ZraI