Restriction map for pF1KA0159
##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        AviII       AvrII       
BbrPI       BbvCI       BfrI        BglI        BlnI        
BsaAI       BsaBI       BseAI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstEII      BstPI       BstUI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
EagI        EclXI       Eco52I      EcoNI       EcoO65I     
EcoT22I     FseI        FspI        HpaI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NheI        NotI        NruI        NsiI        
PacI        PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SgrAI       
SnaBI       SphI        Sse8387I    SspBI       SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1232  GACGTC               GACGT^C
Aor51HI      2309  AGCGCT               AGC^GCT
AseI          631  ATTAAT               AT^TAAT
AsnI          631  ATTAAT               AT^TAAT
AspI         4006  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         2852  GGCGCC               GGCGC^C
BclI         3137  TGATCA               T^GATCA
BglII        4119  AGATCT               A^GATCT
BlpI         3313  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3313  GCTCAGC              GC^T[ACGT]AGC
BsaWI        3212  TCCGGT               [AT]^CCGG[AT]
BsmBI         376  CGTCTC               CGTCTC(1/5)
BsmI          904  GCATTC               GAATGC(1/-1)
BspHI        3566  TCATGA               T^CATGA
BsrFI        2849  GCCGGC               [GA]^CCGG[CT]
BssSI        2345  CACGAG               CACGAG(-5/-1)
BstBI         209  TTCGAA               TT^CGAA
CelII        3313  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       2849  GCCGGC               [GA]^CCGG[CT]
DrdI          848  GACAAAAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2309  AGCGCT               AGC^GCT
EcoRV        3505  GATATC               GAT^ATC
FbaI         3137  TGATCA               T^GATCA
HgaI         1553  GACGC                GACGC(5/10)
HincII       1126  GTCAAC               GT[CT]^[GA]AC
HindII       1126  GTCAAC               GT[CT]^[GA]AC
KasI         2852  GGCGCC               G^GCGCC
NaeI         2849  GCCGGC               GCC^GGC
NarI         2852  GGCGCC               GG^CGCC
NdeI          706  CATATG               CA^TATG
NgoMIV       2849  GCCGGC               G^CCGGC
NspI          953  GCATGT               [GA]CATG^[CT]
NspV          209  TTCGAA               TT^CGAA
PaeR7I       1607  CTCGAG               C^TCGAG
PflFI        4006  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         538  GACATCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         631  ATTAAT               AT^TAAT
RcaI         3566  TCATGA               T^CATGA
SfoI         2852  GGCGCC               GGC^GCC
SfuI          209  TTCGAA               TT^CGAA
SmaI         1745  CCCGGG               CCC^GGG
SpeI         1839  ACTAGT               A^CTAGT
Tth111I      4006  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1607  CTCGAG               C^TCGAG
XmaCI        1745  CCCGGG               C^CCGGG
XmaI         1745  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I       2001  GGTACC               G^GTACC
             3971  GGTACC              
AccI         1461  GTCTAC               GT^[AC][GT]AC
             1752  GTCTAC              
AcyI         1232  GACGTC               G[GA]^CG[CT]C
             2852  GGCGCC              
AflIII        927  ACGTGT               A^C[GA][CT]GT
             1198  ACACGT              
AhdI         1775  GACAGACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3754  GACAAACTGTC         
Asp700I      2477  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3450  GAACTTCTTC          
Asp718I      2001  GGTACC               G^GTACC
             3971  GGTACC              
AspEI        1775  GACAGACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3754  GACAAACTGTC         
BamHI        1809  GGATCC               G^GATCC
             2591  GGATCC              
BcgI          201  GCATCACTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3858  GCAGAAGCTTCG        
BciVI        1098  GGATAC               GTATCC(6/5)
              265  GTATCC              
BsaHI        1232  GACGTC               G[GA]^CG[CT]C
             2852  GGCGCC              
BsgI         1957  GTGCAG               GTGCAG(16/14)
             2227  GTGCAG              
BsrBI        2272  GAGCGG               CCGCTC(-3/-3)
             3658  GAGCGG              
BsrDI        2454  CATTGC               GCAATG(2/0)
             2746  GCAATG              
BstXI        3260  CCAATCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3881  CCAGAGGTTTGG        
Eam1105I     1775  GACAGACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3754  GACAAACTGTC         
EcoRI         247  GAATTC               G^AATTC
             1972  GAATTC              
Hin1I        1232  GACGTC               G[GA]^CG[CT]C
             2852  GGCGCC              
HindIII      1318  AAGCTT               A^AGCTT
             3862  AAGCTT              
KpnI         2001  GGTACC               GGTAC^C
             3971  GGTACC              
PpuMI        2726  AGGACCC              [GA]G^G[AT]CC[CT]
             3305  GGGACCC             
StuI         3075  AGGCCT               AGG^CCT
             3714  AGGCCT              
XmnI         2477  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3450  GAACTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com