Restriction map for pF1KA0159
 (Linear) (Six-base) MAPSORT of: KIAA0159.seq  Check: 731  from: 1  to: 4203



KIAA0159.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     683    2430    4203 
   Size:        683    1747    1773

  Fragments arranged by size: 

                1773    1747     683
 
AatII G_ACGT'C

Cuts at:      0    1236    4203 
   Size:       1236    2967
 
AccI GT'mk_AC

Cuts at:      0    1462    1753    4203 
   Size:       1462     291    2450

  Fragments arranged by size: 

                2450    1462     291
 
Acc65I G'GTAC_C

Cuts at:      0    2001    3971    4203 
   Size:       2001    1970     232

  Fragments arranged by size: 

                2001    1970     232
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     514     866    1559    1629    3762    4203 
   Size:        493      21     352     693      70    2133     441

  Fragments arranged by size: 

                2133     693     493     441     352      70      21
 
AfeI AGC'GCT

Cuts at:      0    2311    4203 
   Size:       2311    1892
 
AflIII A'CryG_T

Cuts at:      0     927    1198    4203 
   Size:        927     271    3005

  Fragments arranged by size: 

                3005     927     271
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1780    3759    4203 
   Size:       1780    1979     444

  Fragments arranged by size: 

                1979    1780     444
 
AleI CACnn'nnGTG

Cuts at:      0    2386    4203 
   Size:       2386    1817
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2693    4203 
   Size:       2693    1510
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2764    2796    4203 
   Size:       2764      32    1407

  Fragments arranged by size: 

                2764    1407      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     741    1281    1408    1869    2013    2265    2691    2811 
   Size:        741     540     127     461     144     252     426     120

Cuts at:   2811    3030    3981    4203 
   Size:        219     951     222

  Fragments arranged by size: 

                 951     741     540     461     426     252     222     219
                 144     127     120
 
ApaI G_GGCC'C

Cuts at:      0     158    1386    2644    4203 
   Size:        158    1228    1258    1559

  Fragments arranged by size: 

                1559    1258    1228     158
 
ApoI r'AATT_y

Cuts at:      0     247     577    1972    2210    3850    4203 
   Size:        247     330    1395     238    1640     353

  Fragments arranged by size: 

                1640    1395     353     330     247     238
 
AseI AT'TA_AT

Cuts at:      0     632    4203 
   Size:        632    3571
 
AvaI C'yCGr_G

Cuts at:      0     656    1073    1607    1745    3421    3529    4203 
   Size:        656     417     534     138    1676     108     674

  Fragments arranged by size: 

                1676     674     656     534     417     138     108
 
BamHI G'GATC_C

Cuts at:      0    1809    2591    4203 
   Size:       1809     782    1612

  Fragments arranged by size: 

                1809    1612     782
 
BanI G'GyrC_C

Cuts at:      0     762    2001    2852    3971    4203 
   Size:        762    1239     851    1119     232

  Fragments arranged by size: 

                1239    1119     851     762     232
 
BanII G_rGCy'C

Cuts at:      0     150     158    1386    2644    2846    2917    3467    4025 
   Size:        150       8    1228    1258     202      71     550     558

Cuts at:   4025    4203 
   Size:        178

  Fragments arranged by size: 

                1258    1228     558     550     202     178     150      71
                   8
 
BbeI G_GCGC'C

Cuts at:      0    2856    4203 
   Size:       2856    1347
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1980    3163    4083    4148    4203 
   Size:       1980    1183     920      65      55

  Fragments arranged by size: 

                1980    1183     920      65      55
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     190     224    3847    3881    4203 
   Size:        190      34    3623      34     322

  Fragments arranged by size: 

                3623     322     190      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     276    1092    4203 
   Size:        276     816    3111

  Fragments arranged by size: 

                3111     816     276
 
BclI T'GATC_A

Cuts at:      0    3137    4203 
   Size:       3137    1066
 
BglII A'GATC_T

Cuts at:      0    4119    4203 
   Size:       4119      84
 
BlpI GC'TnA_GC

Cuts at:      0    3314    4203 
   Size:       3314     889
 
Bme1580I G_kGCm'C

Cuts at:      0     158    1386    2644    2661    4203 
   Size:        158    1228    1258      17    1542

  Fragments arranged by size: 

                1542    1258    1228     158      17
 
BmgBI CAC'GTC

Cuts at:      0    3814    4203 
   Size:       3814     389
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     492    3245    4203 
   Size:        492    2753     958

  Fragments arranged by size: 

                2753     958     492
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    2326    2713    4203 
   Size:        637    1689     387    1490

  Fragments arranged by size: 

                1689    1490     637     387
 
Bpu10I CC'TnA_GC

Cuts at:      0     149    1511    4203 
   Size:        149    1362    2692

  Fragments arranged by size: 

                2692    1362     149
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2224    3346    3928    4080    4203 
   Size:       2224    1122     582     152     123

  Fragments arranged by size: 

                2224    1122     582     152     123
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     388     869    1079    1351    1669    2914    3665    4203 
   Size:        388     481     210     272     318    1245     751     538

  Fragments arranged by size: 

                1245     751     538     481     388     318     272     210
            
 
BsaHI Gr'CG_yC

Cuts at:      0    1233    2853    4203 
   Size:       1233    1620    1350

  Fragments arranged by size: 

                1620    1350    1233
 
BsaWI w'CCGG_w

Cuts at:      0    3212    4203 
   Size:       3212     991
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     818     848    1873    1903    4203 
   Size:        818      30    1025      30    2300

  Fragments arranged by size: 

                2300    1025     818      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58    1441    1444    1513    1516    1678    1687    2947 
   Size:         58    1383       3      69       3     162       9    1260

Cuts at:   2947    2950    3272    3568    4203 
   Size:          3     322     296     635

  Fragments arranged by size: 

                1383    1260     635     322     296     162      69      58
                   9       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     933    1716    2258    2435    2788    2940    3161    3534 
   Size:        933     783     542     177     353     152     221     373

Cuts at:   3534    4055    4203 
   Size:        521     148

  Fragments arranged by size: 

                 933     783     542     521     373     353     221     177
                 152     148
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1978    2248    4203 
   Size:       1978     270    1955

  Fragments arranged by size: 

                1978    1955     270
 
BsiHKAI G_wGCw'C

Cuts at:      0     150    2846    2882    2917    3467    4203 
   Size:        150    2696      36      35     550     736

  Fragments arranged by size: 

                2696     736     550     150      36      35
 
BsmI GAATG_Cn'

Cuts at:      0     904    4203 
   Size:        904    3299
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     382    4203 
   Size:        382    3821
 
Bsp1286I G_dGCh'C

Cuts at:      0     150     158    1386    2644    2661    2846    2882    2917 
   Size:        150       8    1228    1258      17     185      36      35

Cuts at:   2917    3467    4025    4203 
   Size:        550     558     178

  Fragments arranged by size: 

                1258    1228     558     550     185     178     150      36
                  35      17       8
 
BspHI T'CATG_A

Cuts at:      0    3566    4203 
   Size:       3566     637
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     683    1029    2430    2783    3378    3913    4203 
   Size:        683     346    1401     353     595     535     290

  Fragments arranged by size: 

                1401     683     595     535     353     346     290
 
BsrBI CCG'CTC

Cuts at:      0    2274    3660    4203 
   Size:       2274    1386     543

  Fragments arranged by size: 

                2274    1386     543
 
BsrDI GCAATG_nn'

Cuts at:      0    2453    2753    4203 
   Size:       2453     300    1450

  Fragments arranged by size: 

                2453    1450     300
 
BsrFI r'CCGG_y

Cuts at:      0    2849    4203 
   Size:       2849    1354
 
BssSI C'ACGA_G

Cuts at:      0    2345    4203 
   Size:       2345    1858
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1408    1854    3131    4203 
   Size:       1408     446    1277    1072

  Fragments arranged by size: 

                1408    1277    1072     446
 
BstBI TT'CG_AA

Cuts at:      0     210    4203 
   Size:        210    3993
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3267    3888    4203 
   Size:       3267     621     315

  Fragments arranged by size: 

                3267     621     315
 
BstYI r'GATC_y

Cuts at:      0     219    1809    2591    2892    3240    4119    4198    4203 
   Size:        219    1590     782     301     348     879      79       5

  Fragments arranged by size: 

                1590     879     782     348     301     219      79       5
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1814    2519    3827    4203 
   Size:       1814     705    1308     376

  Fragments arranged by size: 

                1814    1308     705     376
 
BtgI C'CryG_G

Cuts at:      0     398     471    1019    1113    2927    2988    4203 
   Size:        398      73     548      94    1814      61    1215

  Fragments arranged by size: 

                1814    1215     548     398      94      73      61
 
BtsI GCAGTG_nn'

Cuts at:      0    1217    1345    1418    2258    2618    2837    4203 
   Size:       1217     128      73     840     360     219    1366

  Fragments arranged by size: 

                1366    1217     840     360     219     128      73
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     401     436    1934    1969    4203 
   Size:        401      35    1498      35    2234

  Fragments arranged by size: 

                2234    1498     401      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     854    4203 
   Size:        854    3349
 
EaeI y'GGCC_r

Cuts at:      0     401    1116    1283    3245    3443    3915    4203 
   Size:        401     715     167    1962     198     472     288

  Fragments arranged by size: 

                1962     715     472     401     288     198     167
 
EarI CTCTTCn'nnn_

Cuts at:      0     485     808    1165    2699    2704    3542    4203 
   Size:        485     323     357    1534       5     838     661

  Fragments arranged by size: 

                1534     838     661     485     357     323       5
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1039    4203 
   Size:       1039    3164
 
EcoICRI GAG'CTC

Cuts at:      0     148    2844    2915    3465    4203 
   Size:        148    2696      71     550     738

  Fragments arranged by size: 

                2696     738     550     148      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     514     637     866    1559    1629    2326    2713 
   Size:        493      21     123     229     693      70     697     387

Cuts at:   2713    3762    4203 
   Size:       1049     441

  Fragments arranged by size: 

                1049     697     693     493     441     387     229     123
                  70      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     154     155    1382    2010    2640    2641    2688    2727 
   Size:        154       1    1227     628     630       1      47      39

Cuts at:   2727    3306    4203 
   Size:        579     897

  Fragments arranged by size: 

                1227     897     630     628     579     154      47      39
                   1       1
 
EcoRI G'AATT_C

Cuts at:      0     247    1972    4203 
   Size:        247    1725    2231

  Fragments arranged by size: 

                2231    1725     247
 
EcoRV GAT'ATC

Cuts at:      0    3507    4203 
   Size:       3507     696
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2095    3471    4203 
   Size:       2095    1376     732

  Fragments arranged by size: 

                2095    1376     732
 
HaeII r_GCGC'y

Cuts at:      0    2313    2856    3940    4203 
   Size:       2313     543    1084     263

  Fragments arranged by size: 

                2313    1084     543     263
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111    1114    1146    1444    1476    1766    1798 
   Size:         79      32    1003      32     298      32     290      32

Cuts at:   1798    2139    2171    2703    2735    2877    2909    3270    3302 
   Size:        341      32     532      32     142      32     361      32

Cuts at:   3302    3745    3777    4203 
   Size:        443      32     426

  Fragments arranged by size: 

                1003     532     443     426     361     341     298     290
                 142      79      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1128    4203 
   Size:       1128    3075
 
HindIII A'AGCT_T

Cuts at:      0    1318    3862    4203 
   Size:       1318    2544     341

  Fragments arranged by size: 

                2544    1318     341
 
KasI G'GCGC_C

Cuts at:      0    2852    4203 
   Size:       2852    1351
 
KpnI G_GTAC'C

Cuts at:      0    2005    3975    4203 
   Size:       2005    1970     228

  Fragments arranged by size: 

                2005    1970     228
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1787    2993    4203 
   Size:        515    1272    1206    1210

  Fragments arranged by size: 

                1272    1210    1206     515
 
MscI TGG'CCA

Cuts at:      0     403    1118    1285    3247    3445    3917    4203 
   Size:        403     715     167    1962     198     472     286

  Fragments arranged by size: 

                1962     715     472     403     286     198     167
 
MslI CAynn'nnrTG

Cuts at:      0     341     736     977    1118    2386    3290    3565    4203 
   Size:        341     395     241     141    1268     904     275     638

  Fragments arranged by size: 

                1268     904     638     395     341     275     241     141
            
 
MspA1I CmG'CkG

Cuts at:      0    1278    2262    2811    3106    3334    3636    3696    4203 
   Size:       1278     984     549     295     228     302      60     507

  Fragments arranged by size: 

                1278     984     549     507     302     295     228      60
            
 
NaeI GCC'GGC

Cuts at:      0    2851    4203 
   Size:       2851    1352
 
NarI GG'CG_CC

Cuts at:      0    2853    4203 
   Size:       2853    1350
 
NcoI C'CATG_G

Cuts at:      0     398     471    1019    1113    2927    4203 
   Size:        398      73     548      94    1814    1276

  Fragments arranged by size: 

                1814    1276     548     398      94      73
 
NdeI CA'TA_TG

Cuts at:      0     707    4203 
   Size:        707    3496
 
NgoMIV G'CCGG_C

Cuts at:      0    2849    4203 
   Size:       2849    1354
 
NspI r_CATG'y

Cuts at:      0     957    4203 
   Size:        957    3246
 
PasI CC'CwG_GG

Cuts at:      0    1491    4203 
   Size:       1491    2712
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1095    1212    1281    3961    3981    4203 
   Size:       1095     117      69    2680      20     222

  Fragments arranged by size: 

                2680    1095     222     117      69      20
 
PfoI T'CCnGG_A

Cuts at:      0     296     380    1628    1891    2066    2864    3185    3302 
   Size:        296      84    1248     263     175     798     321     117

Cuts at:   3302    4203 
   Size:        901

  Fragments arranged by size: 

                1248     901     798     321     296     263     175     117
                  84
 
PpuMI rG'GwC_Cy

Cuts at:      0    2727    3306    4203 
   Size:       2727     579     897

  Fragments arranged by size: 

                2727     897     579
 
PshAI GACnn'nnGTC

Cuts at:      0     542    4203 
   Size:        542    3661
 
PsiI TTA'TAA

Cuts at:      0     701    4203 
   Size:        701    3502
 
PspOMI G'GGCC_C

Cuts at:      0     154    1382    2640    4203 
   Size:        154    1228    1258    1563

  Fragments arranged by size: 

                1563    1258    1228     154
 
PstI C_TGCA'G

Cuts at:      0     173     746    1040    1352    1401    1413    1472    1874 
   Size:        173     573     294     312      49      12      59     402

Cuts at:   1874    2807    3105    4203 
   Size:        933     298    1098

  Fragments arranged by size: 

                1098     933     573     402     312     298     294     173
                  59      49      12
 
PvuII CAG'CTG

Cuts at:      0    1278    2262    2811    3106    3696    4203 
   Size:       1278     984     549     295     590     507

  Fragments arranged by size: 

                1278     984     590     549     507     295
 
SacI G_AGCT'C

Cuts at:      0     150    2846    2917    3467    4203 
   Size:        150    2696      71     550     736

  Fragments arranged by size: 

                2696     736     550     150      71
 
SanDI GG'GwC_CC

Cuts at:      0    3306    4203 
   Size:       3306     897
 
SapI GCTCTTCn'nnn_

Cuts at:      0     808    1165    3542    4203 
   Size:        808     357    2377     661

  Fragments arranged by size: 

                2377     808     661     357
 
SfcI C'TryA_G

Cuts at:      0     169     189     719     742    1036    1348    1397    1409 
   Size:        169      20     530      23     294     312      49      12

Cuts at:   1409    1468    1480    1754    1870    1881    1949    2424    2803 
   Size:         59      12     274     116      11      68     475     379

Cuts at:   2803    3081    3101    3989    4203 
   Size:        278      20     888     214

  Fragments arranged by size: 

                 888     530     475     379     312     294     278     274
                 214     169     116      68      59      49      23      20
                  20      12      12      11
 
SfoI GGC'GCC

Cuts at:      0    2854    4203 
   Size:       2854    1349
 
SmaI CCC'GGG

Cuts at:      0    1747    4203 
   Size:       1747    2456
 
SmlI C'TyrA_G

Cuts at:      0    1607    2203    3361    3907    4095    4203 
   Size:       1607     596    1158     546     188     108

  Fragments arranged by size: 

                1607    1158     596     546     188     108
 
SpeI A'CTAG_T

Cuts at:      0    1839    4203 
   Size:       1839    2364
 
StuI AGG'CCT

Cuts at:      0    3077    3716    4203 
   Size:       3077     639     487

  Fragments arranged by size: 

                3077     639     487
 
StyI C'CwwG_G

Cuts at:      0     398     432     461     471    1019    1113    2132    2897 
   Size:        398      34      29      10     548      94    1019     765

Cuts at:   2897    2927    3120    3593    4203 
   Size:         30     193     473     610

  Fragments arranged by size: 

                1019     765     610     548     473     398     193      94
                  34      30      29      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2591    4069    4167    4203 
   Size:       2591    1478      98      36

  Fragments arranged by size: 

                2591    1478      98      36
 
TatI w'GTAC_w

Cuts at:      0     768    4203 
   Size:        768    3435
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2576    2608    3613    3645    4203 
   Size:       2576      32    1005      32     558

  Fragments arranged by size: 

                2576    1005     558      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    4009    4203 
   Size:       4009     194
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1026    1278    1430    2014    2585    3240    4203 
   Size:       1026     252     152     584     571     655     963

  Fragments arranged by size: 

                1026     963     655     584     571     252     152
 
XhoI C'TCGA_G

Cuts at:      0    1607    4203 
   Size:       1607    2596
 
XmaI C'CCGG_G

Cuts at:      0    1745    4203 
   Size:       1745    2458
 
XmnI GAAnn'nnTTC

Cuts at:      0    2481    3454    4203 
   Size:       2481     973     749

  Fragments arranged by size: 

                2481     973     749
 
ZraI GAC'GTC

Cuts at:      0    1234    4203 
   Size:       1234    2969
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AseI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI     BtsI
    CspCI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI    PshAI
     PsiI   PspOMI     PstI    PvuII     SacI    SanDI     SapI     SfcI
     SfoI     SmaI     SmlI     SpeI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BbvCI    BfrBI     BglI     BmtI     BplI    BsaAI    BsaBI    BsiEI
    BsiWI    BspEI    BsrGI   BssHII   BstEII  BstZ17I    BtgZI     ClaI
     DraI   DraIII     EagI    EcoNI     FseI     FspI    FspAI     HpaI
     MfeI     MluI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SphI     SrfI     SspI     SwaI     XbaI
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