Restriction map for pF1KA0131
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

Acc65I      AclI        AflII       AhdI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BciVI       
BclI        BfrI        BlnI        BsaBI       BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrGI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HpaI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PmeI        PshBI       Psp1406I    RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII         790  GACGTC               GACGT^C
AccI         2113  GTCTAC               GT^[AC][GT]AC
AcsI         1459  AAATTC               [GA]^AATT[CT]
AflIII       2589  ACACGT               A^C[GA][CT]GT
AgeI         1995  ACCGGT               A^CCGGT
Alw44I        714  GTGCAC               G^TGCAC
AocI         2527  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       215  AGCGCT               AGC^GCT
ApaLI         714  GTGCAC               G^TGCAC
ApoI         1459  AAATTC               [GA]^AATT[CT]
BamHI         936  GGATCC               G^GATCC
BglII        1040  AGATCT               A^GATCT
BsmBI          52  GAGACG               CGTCTC(1/5)
BsrDI         402  CATTGC               GCAATG(2/0)
BssHII        724  GCGCGC               G^CGCGC
Bsu36I       2527  CCTGAGG              CC^T[ACGT]AGG
EagI          863  CGGCCG               C^GGCCG
EclXI         863  CGGCCG               C^GGCCG
Eco47III      215  AGCGCT               AGC^GCT
Eco52I        863  CGGCCG               C^GGCCG
Eco81I       2527  CCTGAGG              CC^T[ACGT]AGG
HincII        757  GTCAAC               GT[CT]^[GA]AC
HindII        757  GTCAAC               GT[CT]^[GA]AC
HindIII       264  AAGCTT               A^AGCTT
MseI         2636  TTAA                 T^TAA
MslI         1012  CACCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1268  GCCGGC               GCC^GGC
NcoI         2534  CCATGG               C^CATGG
NgoMIV       1268  GCCGGC               G^CCGGC
PflMI        1713  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1995  ACCGGT               A^CCGGT
PvuI         2073  CGATCG               CGAT^CG
SgrAI        1877  CGCCGGTG             C[GA]^CCGG[CT]G
Tru9I        2636  TTAA                 T^TAA
Tsp509I      1460  AATT                 ^AATT
Van91I       1713  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

BbeI          161  GGCGCC               GGCGC^C
              419  GGCGCC              
BbrPI        1846  CACGTG               CAC^GTG
             2590  CACGTG              
BbvCI        2792  CCTCAGC              CCTCAGC(-5/-2)
              169  GCTGAGG             
BcgI          966  CGAGGTGCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2067  GCAGCCCGATCG        
BfaI          752  CTAG                 C^TAG
             2815  CTAG                
BsaAI        1846  CACGTG               [CT]AC^GT[GA]
             2590  CACGTG              
BsiEI         863  CGGCCG               CG[GA][CT]^CG
             2073  CGATCG              
BsmI          537  GAATGC               GAATGC(1/-1)
             1580  GCATTC              
BssSI        1381  CACGAG               CACGAG(-5/-1)
             2326  CACGAG              
BstAPI        450  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1161  GCAGGCCCTGC         
DsaI          228  CCGTGG               C^C[GA][CT]GG
             2534  CCATGG              
KasI          161  GGCGCC               G^GCGCC
              419  GGCGCC              
MaeI          752  CTAG                 C^TAG
             2815  CTAG                
NarI          161  GGCGCC               GG^CGCC
              419  GGCGCC              
NspI          972  GCATGC               [GA]CATG^[CT]
             2369  GCATGC              
PaeR7I        964  CTCGAG               C^TCGAG
             2337  CTCGAG              
PmaCI        1846  CACGTG               CAC^GTG
             2590  CACGTG              
PmlI         1846  CACGTG               CAC^GTG
             2590  CACGTG              
PshAI          54  GACGCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2655  GACCTCTGTC          
SapI          432  GAAGAGC              GCTCTTC(1/4)
             1467  GAAGAGC             
SfoI          161  GGCGCC               GGC^GCC
              419  GGCGCC              
SmaI          191  CCCGGG               CCC^GGG
             2765  CCCGGG              
SphI          972  GCATGC               GCATG^C
             2369  GCATGC              
TfiI         1071  GATTC                G^A[AT]TC
             1207  GATTC               
XhoI          964  CTCGAG               C^TCGAG
             2337  CTCGAG              
XmaCI         191  CCCGGG               C^CCGGG
             2765  CCCGGG              
XmaI          191  CCCGGG               C^CCGGG
             2765  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspEI       BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MroI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        XhoII       
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