Restriction map for pF1KA0131
 (Linear) (Six-base) MAPSORT of: KIAA0131.seq  Check: 1065  from: 1  to: 2838



KIAA0131.orf

 With 182 enzymes: SgfI * 

                        September 4, 2008 14:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     834    2415    2838 
   Size:        834    1581     423

  Fragments arranged by size: 

                1581     834     423
 
AatII G_ACGT'C

Cuts at:      0     794    2838 
   Size:        794    2044
 
AccI GT'mk_AC

Cuts at:      0    2114    2838 
   Size:       2114     724
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1168    1387    1762    2838 
   Size:       1168     219     375    1076

  Fragments arranged by size: 

                1168    1076     375     219
 
AfeI AGC'GCT

Cuts at:      0     217    2838 
   Size:        217    2621
 
AflIII A'CryG_T

Cuts at:      0    2589    2838 
   Size:       2589     249
 
AgeI A'CCGG_T

Cuts at:      0    1995    2838 
   Size:       1995     843
 
AlwNI CAG_nnn'CTG

Cuts at:      0     144     393     456     903    1167    2292    2838 
   Size:        144     249      63     447     264    1125     546

  Fragments arranged by size: 

                1125     546     447     264     249     144      63
 
ApaI G_GGCC'C

Cuts at:      0     380     635    1985    2711    2773    2781    2838 
   Size:        380     255    1350     726      62       8      57

  Fragments arranged by size: 

                1350     726     380     255      62      57       8
 
ApaLI G'TGCA_C

Cuts at:      0     714    2838 
   Size:        714    2124
 
ApoI r'AATT_y

Cuts at:      0    1459    2838 
   Size:       1459    1379
 
AvaI C'yCGr_G

Cuts at:      0     191     964    2337    2473    2765    2838 
   Size:        191     773    1373     136     292      73

  Fragments arranged by size: 

                1373     773     292     191     136      73
 
BamHI G'GATC_C

Cuts at:      0     936    2838 
   Size:        936    1902
 
BanI G'GyrC_C

Cuts at:      0     161     419     616    1636    1998    2130    2838 
   Size:        161     258     197    1020     362     132     708

  Fragments arranged by size: 

                1020     708     362     258     197     161     132
 
BanII G_rGCy'C

Cuts at:      0     380     470     491     635     649    1086    1546    1769 
   Size:        380      90      21     144      14     437     460     223

Cuts at:   1769    1985    2645    2711    2773    2781    2838 
   Size:        216     660      66      62       8      57

  Fragments arranged by size: 

                 660     460     437     380     223     216     144      90
                  66      62      57      21      14       8
 
BbeI G_GCGC'C

Cuts at:      0     165     423    2838 
   Size:        165     258    2415

  Fragments arranged by size: 

                2415     258     165
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     511     975    2073    2838 
   Size:        511     464    1098     765

  Fragments arranged by size: 

                1098     765     511     464
 
BbvCI CC'TCA_GC

Cuts at:      0     170    2793    2838 
   Size:        170    2623      45

  Fragments arranged by size: 

                2623     170      45
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     955     989    2056    2090    2838 
   Size:        955      34    1067      34     748

  Fragments arranged by size: 

                1067     955     748      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     233     625    2007    2744    2838 
   Size:        233     392    1382     737      94

  Fragments arranged by size: 

                1382     737     392     233      94
 
BglII A'GATC_T

Cuts at:      0    1040    2838 
   Size:       1040    1798
 
BlpI GC'TnA_GC

Cuts at:      0      82     742    2293    2838 
   Size:         82     660    1551     545

  Fragments arranged by size: 

                1551     660     545      82
 
Bme1580I G_kGCm'C

Cuts at:      0     380     635     718    1562    1641    1985    2003    2711 
   Size:        380     255      83     844      79     344      18     708

Cuts at:   2711    2773    2781    2838 
   Size:         62       8      57

  Fragments arranged by size: 

                 844     708     380     344     255      83      79      62
                  57      18       8
 
BmgBI CAC'GTC

Cuts at:      0     414    2838 
   Size:        414    2424
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1437    1488    1923    2693    2838 
   Size:       1437      51     435     770     145

  Fragments arranged by size: 

                1437     770     435     145      51
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     237     498    2352    2838 
   Size:        237     261    1854     486

  Fragments arranged by size: 

                1854     486     261     237
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     207     466     475     493     926    1192    1315 
   Size:        124      83     259       9      18     433     266     123

Cuts at:   1315    1783    1840    2188    2254    2838 
   Size:        468      57     348      66     584

  Fragments arranged by size: 

                 584     468     433     348     266     259     124     123
                  83      66      57      18       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     170    1545    2793    2838 
   Size:        170    1375    1248      45

  Fragments arranged by size: 

                1375    1248     170      45
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637     694    1225    2838 
   Size:        637      57     531    1613

  Fragments arranged by size: 

                1613     637     531      57
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     486    1297    1656    2838 
   Size:        486     811     359    1182

  Fragments arranged by size: 

                1182     811     486     359
 
BsaAI yAC'GTr

Cuts at:      0    1848    2592    2838 
   Size:       1848     744     246

  Fragments arranged by size: 

                1848     744     246
 
BsaHI Gr'CG_yC

Cuts at:      0     162     420     791    2159    2838 
   Size:        162     258     371    1368     679

  Fragments arranged by size: 

                1368     679     371     258     162
 
BsaWI w'CCGG_w

Cuts at:      0     269     851    1754    1995    2838 
   Size:        269     582     903     241     843

  Fragments arranged by size: 

                 903     843     582     269     241
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2679    2709    2838 
   Size:       2679      30     129

  Fragments arranged by size: 

                2679     129      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     589    1144    1435    2838 
   Size:        589     555     291    1403

  Fragments arranged by size: 

                1403     589     555     291
 
BseYI C'CCAG_C

Cuts at:      0     305     386    1486    1640    2005    2162    2571    2731 
   Size:        305      81    1100     154     365     157     409     160

Cuts at:   2731    2781    2838 
   Size:         50      57

  Fragments arranged by size: 

                1100     409     365     305     160     157     154      81
                  57      50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     916    1689    2071    2086    2308    2838 
   Size:        916     773     382      15     222     530

  Fragments arranged by size: 

                 916     773     530     382     222      15
 
BsiEI CG_ry'CG

Cuts at:      0     866    2076    2838 
   Size:        866    1210     762

  Fragments arranged by size: 

                1210     866     762
 
BsiHKAI G_wGCw'C

Cuts at:      0     260     470     491     649     704     718     851    1546 
   Size:        260     210      21     158      55      14     133     695

Cuts at:   1546    1847    2363    2645    2838 
   Size:        301     516     282     193

  Fragments arranged by size: 

                 695     516     301     282     260     210     193     158
                 133      55      21      14
 
BsmI GAATG_Cn'

Cuts at:      0     543    1580    2838 
   Size:        543    1037    1258

  Fragments arranged by size: 

                1258    1037     543
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      46    2838 
   Size:         46    2792
 
Bsp1286I G_dGCh'C

Cuts at:      0     260     380     470     491     635     649     704     718 
   Size:        260     120      90      21     144      14      55      14

Cuts at:    718     851    1086    1546    1562    1641    1769    1847    1985 
   Size:        133     235     460      16      79     128      78     138

Cuts at:   1985    2003    2363    2645    2711    2773    2781    2838 
   Size:         18     360     282      66      62       8      57

  Fragments arranged by size: 

                 460     360     282     260     235     144     138     133
                 128     120      90      79      78      66      62      57
                  55      21      18      16      14      14       8
 
BspEI T'CCGG_A

Cuts at:      0     269     851    1754    2838 
   Size:        269     582     903    1084

  Fragments arranged by size: 

                1084     903     582     269
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     746     791     834    2415    2838 
   Size:        746      45      43    1581     423

  Fragments arranged by size: 

                1581     746     423      45      43
 
BsrBI CCG'CTC

Cuts at:      0       7      30     567    2838 
   Size:          7      23     537    2271

  Fragments arranged by size: 

                2271     537      23       7
 
BsrDI GCAATG_nn'

Cuts at:      0     401    2838 
   Size:        401    2437
 
BsrFI r'CCGG_y

Cuts at:      0    1268    1878    1995    2504    2838 
   Size:       1268     610     117     509     334

  Fragments arranged by size: 

                1268     610     509     334     117
 
BssHII G'CGCG_C

Cuts at:      0     724    2838 
   Size:        724    2114
 
BssSI C'ACGA_G

Cuts at:      0    1381    2326    2838 
   Size:       1381     945     512

  Fragments arranged by size: 

                1381     945     512
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     456    1167    2838 
   Size:        456     711    1671

  Fragments arranged by size: 

                1671     711     456
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     696    2151    2674    2838 
   Size:        696    1455     523     164

  Fragments arranged by size: 

                1455     696     523     164
 
BstYI r'GATC_y

Cuts at:      0     441     936    1040    1658    2838 
   Size:        441     495     104     618    1180

  Fragments arranged by size: 

                1180     618     495     441     104
 
Bsu36I CC'TnA_GG

Cuts at:      0    2528    2838 
   Size:       2528     310
 
BtgI C'CryG_G

Cuts at:      0     228    2534    2838 
   Size:        228    2306     304

  Fragments arranged by size: 

                2306     304     228
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1952    2838 
   Size:       1952     886
 
BtsI GCAGTG_nn'

Cuts at:      0     300    1156    1706    2838 
   Size:        300     856     550    1132

  Fragments arranged by size: 

                1132     856     550     300
 
EaeI y'GGCC_r

Cuts at:      0       2     209     371     497     863    1290    1730    1967 
   Size:          2     207     162     126     366     427     440     237

Cuts at:   1967    2506    2838 
   Size:        539     332

  Fragments arranged by size: 

                 539     440     427     366     332     237     207     162
                 126       2
 
EagI C'GGCC_G

Cuts at:      0     863    2838 
   Size:        863    1975
 
EarI CTCTTCn'nnn_

Cuts at:      0     427    1462    1780    1909    2838 
   Size:        427    1035     318     129     929

  Fragments arranged by size: 

                1035     929     427     318     129
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2130    2197    2653    2838 
   Size:       2130      67     456     185

  Fragments arranged by size: 

                2130     456     185      67
 
EcoICRI GAG'CTC

Cuts at:      0     468     489     647    1544    2643    2838 
   Size:        468      21     158     897    1099     195

  Fragments arranged by size: 

                1099     897     468     195     158      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     207     466     475     493     926    1168    1192 
   Size:        124      83     259       9      18     433     242      24

Cuts at:   1192    1315    1387    1762    1783    1840    2188    2254    2838 
   Size:        123      72     375      21      57     348      66     584

  Fragments arranged by size: 

                 584     433     375     348     259     242     124     123
                  83      72      66      57      24      21      18       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     361     655     841    1171    2838 
   Size:        361     294     186     330    1667

  Fragments arranged by size: 

                1667     361     330     294     186
 
EcoO109I rG'GnC_Cy

Cuts at:      0     195     377     631     632     900     930    1164    1398 
   Size:        195     182     254       1     268      30     234     234

Cuts at:   1398    1686    2013    2333    2376    2478    2539    2670    2708 
   Size:        288     327     320      43     102      61     131      38

Cuts at:   2708    2724    2769    2770    2777    2778    2811    2838 
   Size:         16      45       1       7       1      33      27

  Fragments arranged by size: 

                 327     320     288     268     254     234     234     195
                 182     131     102      61      45      43      38      33
                  30      27      16       7       1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1767    2661    2838 
   Size:       1767     894     177

  Fragments arranged by size: 

                1767     894     177
 
HaeII r_GCGC'y

Cuts at:      0     165     219     393     423    2026    2838 
   Size:        165      54     174      30    1603     812

  Fragments arranged by size: 

                1603     812     174     165      54      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     426     458    1340    1372    1708    1740    2838 
   Size:        426      32     882      32     336      32    1098

  Fragments arranged by size: 

                1098     882     426     336      32      32      32
 
HincII GTy'rAC

Cuts at:      0     759    2838 
   Size:        759    2079
 
HindIII A'AGCT_T

Cuts at:      0     264    2838 
   Size:        264    2574
 
KasI G'GCGC_C

Cuts at:      0     161     419    2838 
   Size:        161     258    2419

  Fragments arranged by size: 

                2419     258     161
 
MslI CAynn'nnrTG

Cuts at:      0    1016    2838 
   Size:       1016    1822
 
MspA1I CmG'CkG

Cuts at:      0      81      93     456     613     868     996    1831    2005 
   Size:         81      12     363     157     255     128     835     174

Cuts at:   2005    2046    2147    2166    2838 
   Size:         41     101      19     672

  Fragments arranged by size: 

                 835     672     363     255     174     157     128     101
                  81      41      19      12
 
NaeI GCC'GGC

Cuts at:      0    1270    2838 
   Size:       1270    1568
 
NarI GG'CG_CC

Cuts at:      0     162     420    2838 
   Size:        162     258    2418

  Fragments arranged by size: 

                2418     258     162
 
NcoI C'CATG_G

Cuts at:      0    2534    2838 
   Size:       2534     304
 
NgoMIV G'CCGG_C

Cuts at:      0    1268    2838 
   Size:       1268    1570
 
NspI r_CATG'y

Cuts at:      0     976    2373    2838 
   Size:        976    1397     465

  Fragments arranged by size: 

                1397     976     465
 
PasI CC'CwG_GG

Cuts at:      0     837    2773    2808    2838 
   Size:        837    1936      35      30

  Fragments arranged by size: 

                1936     837      35      30
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1719    2838 
   Size:       1719    1119
 
PfoI T'CCnGG_A

Cuts at:      0     470     554    1331    2838 
   Size:        470      84     777    1507

  Fragments arranged by size: 

                1507     777     470      84
 
PmlI CAC'GTG

Cuts at:      0    1848    2592    2838 
   Size:       1848     744     246

  Fragments arranged by size: 

                1848     744     246
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2611    2643    2838 
   Size:       2611      32     195

  Fragments arranged by size: 

                2611     195      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1686    2013    2539    2838 
   Size:       1686     327     526     299

  Fragments arranged by size: 

                1686     526     327     299
 
PshAI GACnn'nnGTC

Cuts at:      0      58    2659    2838 
   Size:         58    2601     179

  Fragments arranged by size: 

                2601     179      58
 
PspOMI G'GGCC_C

Cuts at:      0     376     631    1981    2707    2769    2777    2838 
   Size:        376     255    1350     726      62       8      61

  Fragments arranged by size: 

                1350     726     376     255      62      61       8
 
PspXI vC'TCGA_Gb

Cuts at:      0    2337    2838 
   Size:       2337     501
 
PstI C_TGCA'G

Cuts at:      0      41     113     140     323     461    1163    1373    1394 
   Size:         41      72      27     183     138     702     210      21

Cuts at:   1394    1610    2030    2447    2454    2838 
   Size:        216     420     417       7     384

  Fragments arranged by size: 

                 702     420     417     384     216     210     183     138
                  72      41      27      21       7
 
PvuI CG_AT'CG

Cuts at:      0    2076    2838 
   Size:       2076     762
 
PvuII CAG'CTG

Cuts at:      0      81      93     456    2046    2147    2166    2838 
   Size:         81      12     363    1590     101      19     672

  Fragments arranged by size: 

                1590     672     363     101      81      19      12
 
SacI G_AGCT'C

Cuts at:      0     470     491     649    1546    2645    2838 
   Size:        470      21     158     897    1099     193

  Fragments arranged by size: 

                1099     897     470     193     158      21
 
SanDI GG'GwC_CC

Cuts at:      0    2539    2838 
   Size:       2539     299
 
SapI GCTCTTCn'nnn_

Cuts at:      0     427    1462    2838 
   Size:        427    1035    1376

  Fragments arranged by size: 

                1376    1035     427
 
SfcI C'TryA_G

Cuts at:      0      37     109     136     319     457    1159    1369    1390 
   Size:         37      72      27     183     138     702     210      21

Cuts at:   1390    1606    2026    2443    2450    2838 
   Size:        216     420     417       7     388

  Fragments arranged by size: 

                 702     420     417     388     216     210     183     138
                  72      37      27      21       7
 
SfoI GGC'GCC

Cuts at:      0     163     421    2838 
   Size:        163     258    2417

  Fragments arranged by size: 

                2417     258     163
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1878    2838 
   Size:       1878     960
 
SmaI CCC'GGG

Cuts at:      0     193    2767    2838 
   Size:        193    2574      71

  Fragments arranged by size: 

                2574     193      71
 
SmlI C'TyrA_G

Cuts at:      0     652     709     964    1240    2337    2838 
   Size:        652      57     255     276    1097     501

  Fragments arranged by size: 

                1097     652     501     276     255      57
 
SphI G_CATG'C

Cuts at:      0     976    2373    2838 
   Size:        976    1397     465

  Fragments arranged by size: 

                1397     976     465
 
StyI C'CwwG_G

Cuts at:      0     956    2534    2673    2838 
   Size:        956    1578     139     165

  Fragments arranged by size: 

                1578     956     165     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     895    1124    1321    2838 
   Size:        895     229     197    1517

  Fragments arranged by size: 

                1517     895     229     197
 
TatI w'GTAC_w

Cuts at:      0    1445    1931    2838 
   Size:       1445     486     907

  Fragments arranged by size: 

                1445     907     486
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1018    1510    2739    2838 
   Size:       1018     492    1229      99

  Fragments arranged by size: 

                1229    1018     492      99
 
XhoI C'TCGA_G

Cuts at:      0     964    2337    2838 
   Size:        964    1373     501

  Fragments arranged by size: 

                1373     964     501
 
XmaI C'CCGG_G

Cuts at:      0     191    2765    2838 
   Size:        191    2574      73

  Fragments arranged by size: 

                2574     191      73
 
ZraI GAC'GTC

Cuts at:      0     792    2838 
   Size:        792    2046
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII  HindIII     KasI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI
    PvuII     SacI    SanDI     SapI     SfcI     SfoI    SgrAI     SmaI
     SmlI     SphI     StyI    TaqII     TatI     XcmI     XhoI     XmaI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AhdI     AleI     AlfI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI     BmtI
    BsaBI    BsiWI    BspHI    BsrGI    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV     FseI     FspI
    FspAI     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PsiI
     PsrI    RsrII    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SgfI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XmnI
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