Restriction map for pF1KA0116
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstYI       BstZ17I     Bsu36I      CfoI        
Cfr10I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HhaI        HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       Sse8387I    SspI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          355  GTCGAC               GT^[AC][GT]AC
ApaI          841  GGGCCC               GGGCC^C
AspHI         388  GAGCAC               G[AT]GC[AT]^C
AvaI          383  CTCGGG               C^[CT]CG[GA]G
BalI          668  TGGCCA               TGG^CCA
BanI          304  GGCACC               G^G[CT][GA]CC
BanII         841  GGGCCC               G[GA]GC[CT]^C
BbsI           58  GAAGAC               GAAGAC(2/6)
BcgI          305  GCACCGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         491  GGATAC               GTATCC(6/5)
BfaI          134  CTAG                 C^TAG
BlpI           15  GCTGAGC              GC^T[ACGT]AGC
BpmI          211  CTGGAG               CTGGAG(16/14)
Bpu1102I       15  GCTGAGC              GC^T[ACGT]AGC
BpuAI          58  GAAGAC               GAAGAC(2/6)
BsgI          820  CTGCAC               GTGCAG(16/14)
BsiHKAI       388  GAGCAC               G[AT]GC[AT]^C
BsoBI         383  CTCGGG               C^[CT]CG[GA]G
Bsp1407I       35  TGTACA               T^GTACA
BsrDI         459  CATTGC               GCAATG(2/0)
BsrGI          35  TGTACA               T^GTACA
BstEII        687  GGTGACC              G^GT[ACGT]ACC
BstPI         687  GGTGACC              G^GT[ACGT]ACC
BstXI         671  CCAGCTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII          15  GCTGAGC              GC^T[ACGT]AGC
DpnII         312  GATC                 ^GATC
DraII         840  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DraIII        603  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           78  CCGTGG               C^C[GA][CT]GG
EcoO109I      840  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       687  GGTGACC              G^GT[ACGT]ACC
HinfI         863  GATTC                G^A[ACGT]TC
MaeI          134  CTAG                 C^TAG
MaeII         709  ACGT                 A^CGT
MboI          312  GATC                 ^GATC
MluNI         668  TGGCCA               TGG^CCA
MscI          668  TGGCCA               TGG^CCA
MslI          171  CATCTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         312  GATC                 ^GATC
SalI          355  GTCGAC               G^TCGAC
Sau3AI        312  GATC                 ^GATC
SpeI          133  ACTAGT               A^CTAGT
SphI          798  GCATGC               GCATG^C
SspBI          35  TGTACA               T^GTACA
StuI          656  AGGCCT               AGG^CCT
TfiI          863  GATTC                G^A[AT]TC
XcmI          146  CCAGGGTCAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI          143  CCGC                 CCGC(-3/-1)
               25  GCGG                
AcsI          274  GAATTT               [GA]^AATT[CT]
              438  AAATTT              
AfaI           36  GTAC                 GT^AC
              793  GTAC                
ApoI          274  GAATTT               [GA]^AATT[CT]
              438  AAATTT              
AvaII         140  GGTCC                G^G[AT]CC
              741  GGACC               
BmyI          388  GAGCAC               G[AGT]GC[ACT]^C
              841  GGGCCC              
BseRI         287  GAGGAG               GAGGAG(10/8)
              652  GAGGAG              
BsmAI         769  GAGAC                GTCTC(1/5)
              849  GAGAC               
BsmFI         199  GGGAC                GGGAC(10/14)
              589  GTCCC               
Bsp1286I      388  GAGCAC               G[AGT]GC[ACT]^C
              841  GGGCCC              
BsrI          772  ACTGG                ACTGG(1/-1)
              260  CCAGT               
DdeI           16  CTGAG                C^T[ACGT]AG
              574  CTAAG               
EaeI           75  TGGCCG               [CT]^GGCC[GA]
              668  TGGCCA              
EcoT14I       300  CCTTGG               C^C[AT][AT]GG
              496  CCAAGG              
HincII        247  GTTGAC               GT[CT]^[GA]AC
              355  GTCGAC              
HindII        247  GTTGAC               GT[CT]^[GA]AC
              355  GTCGAC              
HphI          292  GGTGA                GGTGA(8/7)
              687  GGTGA               
MseI          338  TTAA                 T^TAA
              361  TTAA                
MwoI          463  GCTGTAAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              611  GCAAGATTGGC         
NspI          627  GCATGT               [GA]CATG^[CT]
              798  GCATGC              
RsaI           36  GTAC                 GT^AC
              793  GTAC                
StyI          300  CCTTGG               C^C[AT][AT]GG
              496  CCAAGG              
Tru9I         338  TTAA                 T^TAA
              361  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AcyI        AluI        BbvI        BsaHI       BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HgaI        Hin1I       ItaI        
Ksp632I     MaeIII      MboII       MnlI        MvaI        
NlaIII      NlaIV       Sau96I      ScrFI       SfaNI       
TaqI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     
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