Restriction map for
pF1KA0116
(Circular) (Six-base) MAPSORT of: pF1KA0116.seq Check: 8456 from: 1 to: 3986
pF1KA0116 3986 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 464 1024 464
Size: 560 3426
Acc65I G'GTAC_C
Cuts at: 1002 1002
Size: 3986
AclI AA'CG_TT
Cuts at: 3826 3826
Size: 3986
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1811 2243 2596 3258 739
Size: 1072 432 353 662 1467
Fragments arranged by size:
1467 1072 662 432 353
AflIII A'CryG_T
Cuts at: 1338 3138 3419 1338
Size: 1800 281 1905
Fragments arranged by size:
1905 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 919 919
Size: 3986
AlwNI CAG_nnn'CTG
Cuts at: 1291 2729 1291
Size: 1438 2548
ApaI G_GGCC'C
Cuts at: 953 953
Size: 3986
ApaLI G'TGCA_C
Cuts at: 2824 2824
Size: 3986
ApoI r'AATT_y
Cuts at: 382 546 990 3339 3595 382
Size: 164 444 2349 256 773
Fragments arranged by size:
2349 773 444 256 164
AseI AT'TA_AT
Cuts at: 20 20
Size: 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
AvaI C'yCGr_G
Cuts at: 491 1006 491
Size: 515 3471
BamHI G'GATC_C
Cuts at: 1011 1011
Size: 3986
BanI G'GyrC_C
Cuts at: 412 1002 1416 1664 1699 412
Size: 590 414 248 35 2699
Fragments arranged by size:
2699 590 414 248 35
BanII G_rGCy'C
Cuts at: 953 1000 2030 953
Size: 47 1030 2909
Fragments arranged by size:
2909 1030 47
BbeI G_GCGC'C
Cuts at: 1420 1668 1420
Size: 248 3738
BbsI GAAGACnn'nnnn_
Cuts at: 173 173
Size: 3986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 402 436 402
Size: 34 3952
BciVI GTATCCnnnnn_n'
Cuts at: 593 1884 2940 3986 593
Size: 1291 1056 1046 593
Fragments arranged by size:
1291 1056 1046 593
BclI T'GATC_A
Cuts at: 1506 1506
Size: 3986
BglII A'GATC_T
Cuts at: 1501 3538 1501
Size: 2037 1949
BlpI GC'TnA_GC
Cuts at: 124 1098 124
Size: 974 3012
Bme1580I G_kGCm'C
Cuts at: 953 1611 1704 2828 953
Size: 658 93 1124 2111
Fragments arranged by size:
2111 1124 658 93
BmgBI CAC'GTC
Cuts at: 818 818
Size: 3986
BmrI ACTGGGnnnn_n'
Cuts at: 1198 1279 1614 3758 1198
Size: 81 335 2144 1426
Fragments arranged by size:
2144 1426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 786 2353 786
Size: 1567 2419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 340
Size: 3986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 2041 2755 3053 1162
Size: 879 714 298 2095
Fragments arranged by size:
2095 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3690 3690
Size: 3986
BsaAI yAC'GTr
Cuts at: 1339 1339
Size: 3986
BsaBI GATnn'nnATC
Cuts at: 6 1500 3537 6
Size: 1494 2037 455
Fragments arranged by size:
2037 1494 455
BsaHI Gr'CG_yC
Cuts at: 112 158 310 1417 1665 2367 3863 112
Size: 46 152 1107 248 702 1496 235
Fragments arranged by size:
1496 1107 702 248 235 152 46
BsaWI w'CCGG_w
Cuts at: 1175 1696 2374 2785 2932 3206 1175
Size: 521 678 411 147 274 1955
Fragments arranged by size:
1955 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 3956
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 410 775 410
Size: 365 3621
BseYI C'CCAG_C
Cuts at: 264 503 1411 2169 2834 3242 264
Size: 239 908 758 665 408 1008
Fragments arranged by size:
1008 908 758 665 408 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 913
Size: 3986
BsiEI CG_ry'CG
Cuts at: 104 1528 1574 2804 104
Size: 1424 46 1230 1286
Fragments arranged by size:
1424 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 500 1000 1778 1968 2828 500
Size: 500 778 190 860 1658
Fragments arranged by size:
1658 860 778 500 190
Bsp1286I G_dGCh'C
Cuts at: 500 953 1000 1611 1704 1778 1968 2030 2828
Size: 453 47 611 93 74 190 62 798
Cuts at: 2828 500
Size: 1658
Fragments arranged by size:
1658 798 611 453 190 93 74 62
47
BspHI T'CATG_A
Cuts at: 2418 2418
Size: 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1036 1250 1552 1933 1036
Size: 214 302 381 3089
Fragments arranged by size:
3089 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2278 2332 3816 3981 50
Size: 2228 54 1484 165 55
Fragments arranged by size:
2228 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 566 1898 566
Size: 1332 2654
BsrFI r'CCGG_y
Cuts at: 1984 2165 1984
Size: 181 3805
BsrGI T'GTAC_A
Cuts at: 143 143
Size: 3986
BssSI C'ACGA_G
Cuts at: 2257 2965 2257
Size: 708 3278
BstAPI GCAn_nnn'nTGC
Cuts at: 1363 1363
Size: 3986
BstBI TT'CG_AA
Cuts at: 2347 2347
Size: 3986
BstEII G'GTnAC_C
Cuts at: 795 795
Size: 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 786 786
Size: 3986
BstYI r'GATC_y
Cuts at: 1 1011 1478 1501 1836 2082 2388 2400 2486
Size: 1010 467 23 335 246 306 12 86
Cuts at: 2486 3538 1
Size: 1052 449
Fragments arranged by size:
1052 1010 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 186 107
Size: 79 3907
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1936 2117 2280 1936
Size: 181 163 3642
Fragments arranged by size:
3642 181 163
BtsI GCAGTG_nn'
Cuts at: 465 498 1229 465
Size: 33 731 3222
Fragments arranged by size:
3222 731 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 225 260 225
Size: 35 3951
DraI TTT'AAA
Cuts at: 985 985
Size: 3986
DraIII CAC_nnn'GTG
Cuts at: 716 716
Size: 3986
DrdI GACnn_nn'nnGTC
Cuts at: 1692 3036 1692
Size: 1344 2642
EaeI y'GGCC_r
Cuts at: 183 776 1571 1745 2136 2163 3508 183
Size: 593 795 174 391 27 1345 661
Fragments arranged by size:
1345 795 661 593 391 174 27
EagI C'GGCC_G
Cuts at: 1571 1571
Size: 3986
EarI CTCTTCn'nnn_
Cuts at: 592 623 758 2009 2219 592
Size: 31 135 1251 210 2359
Fragments arranged by size:
2359 1251 210 135 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 240 2194 2936 3082 3796 148
Size: 92 1954 742 146 714 338
Fragments arranged by size:
1954 742 714 338 146 92
EcoICRI GAG'CTC
Cuts at: 998 998
Size: 3986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 739 1811 2243 2596 3258 340
Size: 399 1072 432 353 662 1068
Fragments arranged by size:
1072 1068 662 432 399 353
EcoO109I rG'GnC_Cy
Cuts at: 949 1125 949
Size: 176 3810
EcoRI G'AATT_C
Cuts at: 990 990
Size: 3986
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3986
FspI TGC'GCA
Cuts at: 1767 1767
Size: 3986
HaeII r_GCGC'y
Cuts at: 1420 1668 2898 3669 1420
Size: 248 1230 771 1737
Fragments arranged by size:
1737 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 714 746 1828 1858 1860 1890 217
Size: 32 465 32 1082 30 2 30 2313
Fragments arranged by size:
2313 1082 465 32 32 30 30 2
HincII GTy'rAC
Cuts at: 357 465 1025 357
Size: 108 560 3318
Fragments arranged by size:
3318 560 108
KasI G'GCGC_C
Cuts at: 1416 1664 1416
Size: 248 3738
KpnI G_GTAC'C
Cuts at: 1006 1006
Size: 3986
MluI A'CGCG_T
Cuts at: 3419 3419
Size: 3986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 260 948 2745 2929 260
Size: 688 1797 184 1317
Fragments arranged by size:
1797 1317 688 184
MscI TGG'CCA
Cuts at: 778 1747 778
Size: 969 3017
MslI CAynn'nnrTG
Cuts at: 283 1975 2102 283
Size: 1692 127 2167
Fragments arranged by size:
2167 1692 127
MspA1I CmG'CkG
Cuts at: 1097 1411 1771 2555 2800 1097
Size: 314 360 784 245 2283
Fragments arranged by size:
2283 784 360 314 245
NaeI GCC'GGC
Cuts at: 2167 2167
Size: 3986
NarI GG'CG_CC
Cuts at: 1417 1665 1417
Size: 248 3738
NcoI C'CATG_G
Cuts at: 107 107
Size: 3986
NgoMIV G'CCGG_C
Cuts at: 2165 2165
Size: 3986
NspI r_CATG'y
Cuts at: 739 910 1039 1539 3142 739
Size: 171 129 500 1603 1583
Fragments arranged by size:
1603 1583 500 171 129
PciI A'CATG_T
Cuts at: 3138 3138
Size: 3986
PfoI T'CCnGG_A
Cuts at: 161 161
Size: 3986
PmeI GTTT'AAAC
Cuts at: 985 985
Size: 3986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2432 2464 2432
Size: 32 3954
PspOMI G'GGCC_C
Cuts at: 949 949
Size: 3986
PstI C_TGCA'G
Cuts at: 1033 1718 1033
Size: 685 3301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3986
PvuII CAG'CTG
Cuts at: 1411 1771 1411
Size: 360 3626
RsrII CG'GwC_CG
Cuts at: 2181 2181
Size: 3986
SacI G_AGCT'C
Cuts at: 1000 1000
Size: 3986
SalI G'TCGA_C
Cuts at: 463 1023 463
Size: 560 3426
SapI GCTCTTCn'nnn_
Cuts at: 2009 2219 2009
Size: 210 3776
SbfI CC_TGCA'GG
Cuts at: 1033 1033
Size: 3986
ScaI AGT'ACT
Cuts at: 3503 3503
Size: 3986
SfcI C'TryA_G
Cuts at: 33 1029 1714 2682 2873 33
Size: 996 685 968 191 1146
Fragments arranged by size:
1146 996 968 685 191
SfoI GGC'GCC
Cuts at: 1418 1666 1418
Size: 248 3738
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3986
SmaI CCC'GGG
Cuts at: 1008 1008
Size: 3986
SmlI C'TyrA_G
Cuts at: 1141 2056 2770 3032 1141
Size: 915 714 262 2095
Fragments arranged by size:
2095 915 714 262
SpeI A'CTAG_T
Cuts at: 67 241 67
Size: 174 3812
SphI G_CATG'C
Cuts at: 910 1039 1539 910
Size: 129 500 3357
Fragments arranged by size:
3357 500 129
StuI AGG'CCT
Cuts at: 766 766
Size: 3986
StyI C'CwwG_G
Cuts at: 107 408 604 1120 1474 107
Size: 301 196 516 354 2619
Fragments arranged by size:
2619 516 354 301 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1569 1702 2161 2370 1569
Size: 133 459 209 3185
Fragments arranged by size:
3185 459 209 133
TatI w'GTAC_w
Cuts at: 143 3501 143
Size: 3358 628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 3954
Tth111I GACn'n_nGTC
Cuts at: 1783 1783
Size: 3986
XbaI T'CTAG_A
Cuts at: 1017 1017
Size: 3986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 1481 261
Size: 1220 2766
XmaI C'CCGG_G
Cuts at: 1006 1006
Size: 3986
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsmBI BspEI BssHII BstZ17I Bsu36I ClaI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI