Restriction map for
pF1KA0116
(Linear) (Six-base) MAPSORT of: KIAA0116.seq Check: 783 from: 1 to: 873
KIAA0116.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AccI GT'mk_AC
Cuts at: 0 356 873
Size: 356 517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 873
Size: 631 242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 873
Size: 811 62
ApaI G_GGCC'C
Cuts at: 0 845 873
Size: 845 28
ApoI r'AATT_y
Cuts at: 0 274 438 873
Size: 274 164 435
Fragments arranged by size:
435 274 164
AvaI C'yCGr_G
Cuts at: 0 383 873
Size: 383 490
BanI G'GyrC_C
Cuts at: 0 304 873
Size: 304 569
BanII G_rGCy'C
Cuts at: 0 845 873
Size: 845 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 65 873
Size: 65 808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 294 328 873
Size: 294 34 545
Fragments arranged by size:
545 294 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 485 873
Size: 485 388
BlpI GC'TnA_GC
Cuts at: 0 16 873
Size: 16 857
Bme1580I G_kGCm'C
Cuts at: 0 845 873
Size: 845 28
BmgBI CAC'GTC
Cuts at: 0 710 873
Size: 710 163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 678 873
Size: 678 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 873
Size: 232 641
BsaHI Gr'CG_yC
Cuts at: 0 4 50 202 873
Size: 4 46 152 671
Fragments arranged by size:
671 152 46 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 873
Size: 19 30 824
Fragments arranged by size:
824 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 302 667 873
Size: 302 365 206
Fragments arranged by size:
365 302 206
BseYI C'CCAG_C
Cuts at: 0 156 395 873
Size: 156 239 478
Fragments arranged by size:
478 239 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 873
Size: 805 68
BsiHKAI G_wGCw'C
Cuts at: 0 392 873
Size: 392 481
Bsp1286I G_dGCh'C
Cuts at: 0 392 845 873
Size: 392 453 28
Fragments arranged by size:
453 392 28
BsrDI GCAATG_nn'
Cuts at: 0 458 873
Size: 458 415
BsrGI T'GTAC_A
Cuts at: 0 35 873
Size: 35 838
BstEII G'GTnAC_C
Cuts at: 0 687 873
Size: 687 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 873
Size: 678 195
BtgI C'CryG_G
Cuts at: 0 78 873
Size: 78 795
BtsI GCAGTG_nn'
Cuts at: 0 357 390 873
Size: 357 33 483
Fragments arranged by size:
483 357 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 117 152 873
Size: 117 35 721
Fragments arranged by size:
721 117 35
DraIII CAC_nnn'GTG
Cuts at: 0 608 873
Size: 608 265
EaeI y'GGCC_r
Cuts at: 0 75 668 873
Size: 75 593 205
Fragments arranged by size:
593 205 75
EarI CTCTTCn'nnn_
Cuts at: 0 484 515 650 873
Size: 484 31 135 223
Fragments arranged by size:
484 223 135 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 132 873
Size: 40 92 741
Fragments arranged by size:
741 92 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 631 873
Size: 232 399 242
Fragments arranged by size:
399 242 232
EcoO109I rG'GnC_Cy
Cuts at: 0 841 873
Size: 841 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 606 638 873
Size: 109 32 465 32 235
Fragments arranged by size:
465 235 109 32 32
HincII GTy'rAC
Cuts at: 0 249 357 873
Size: 249 108 516
Fragments arranged by size:
516 249 108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 840 873
Size: 152 688 33
Fragments arranged by size:
688 152 33
MscI TGG'CCA
Cuts at: 0 670 873
Size: 670 203
MslI CAynn'nnrTG
Cuts at: 0 175 873
Size: 175 698
NspI r_CATG'y
Cuts at: 0 631 802 873
Size: 631 171 71
Fragments arranged by size:
631 171 71
PfoI T'CCnGG_A
Cuts at: 0 53 873
Size: 53 820
PspOMI G'GGCC_C
Cuts at: 0 841 873
Size: 841 32
SalI G'TCGA_C
Cuts at: 0 355 873
Size: 355 518
SpeI A'CTAG_T
Cuts at: 0 133 873
Size: 133 740
SphI G_CATG'C
Cuts at: 0 802 873
Size: 802 71
StuI AGG'CCT
Cuts at: 0 658 873
Size: 658 215
StyI C'CwwG_G
Cuts at: 0 300 496 873
Size: 300 196 377
Fragments arranged by size:
377 300 196
TatI w'GTAC_w
Cuts at: 0 35 873
Size: 35 838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 873
Size: 153 720
Enzymes that do cut:
AccI AcuI AlfI ApaI ApoI AvaI BanI BanII
BbsI BcgI BciVI BlpI Bme1580I BmgBI BplI BpmI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BsrGI BstEII BstXI BtgI BtsI CspCI DraIII EaeI
EarI EciI Eco57MI EcoO109I Hin4I HincII MmeI MscI
MslI NspI PfoI PspOMI SalI SpeI SphI StuI
StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglI
BglII BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstYI BstZ17I
Bsu36I BtgZI ClaI DraI DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI
ZraI