Restriction map for pF1KA0095
 (Linear) (Six-base) MAPSORT of: KIAA0095.seq  Check: 2456  from: 1  to: 2457



KIAA0095.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     608    2457 
   Size:        608    1849
 
AatII G_ACGT'C

Cuts at:      0    1196    2457 
   Size:       1196    1261
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      13     325     745    1267    1516    2008    2457 
   Size:         13     312     420     522     249     492     449

  Fragments arranged by size: 

                 522     492     449     420     312     249      13
 
AfeI AGC'GCT

Cuts at:      0    2146    2457 
   Size:       2146     311
 
AflIII A'CryG_T

Cuts at:      0     665     695    1691    2457 
   Size:        665      30     996     766

  Fragments arranged by size: 

                 996     766     665      30
 
AleI CACnn'nnGTG

Cuts at:      0     811    2457 
   Size:        811    1646
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2047    2079    2457 
   Size:       2047      32     378

  Fragments arranged by size: 

                2047     378      32
 
ApaI G_GGCC'C

Cuts at:      0    1134    2457 
   Size:       1134    1323
 
ApoI r'AATT_y

Cuts at:      0     428     563     828    2047    2205    2457 
   Size:        428     135     265    1219     158     252

  Fragments arranged by size: 

                1219     428     265     252     158     135
 
AvaI C'yCGr_G

Cuts at:      0     190     197    2457 
   Size:        190       7    2260

  Fragments arranged by size: 

                2260     190       7
 
BanI G'GyrC_C

Cuts at:      0     855    1279    2157    2457 
   Size:        855     424     878     300

  Fragments arranged by size: 

                 878     855     424     300
 
BanII G_rGCy'C

Cuts at:      0      26    1023    1134    1942    2028    2457 
   Size:         26     997     111     808      86     429

  Fragments arranged by size: 

                 997     808     429     111      86      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     464    1343    2457 
   Size:        464     879    1114

  Fragments arranged by size: 

                1114     879     464
 
BbvCI CC'TCA_GC

Cuts at:      0     374    1537    2457 
   Size:        374    1163     920

  Fragments arranged by size: 

                1163     920     374
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2415    2449    2457 
   Size:       2415      34       8

  Fragments arranged by size: 

                2415      34       8
 
BclI T'GATC_A

Cuts at:      0    2084    2457 
   Size:       2084     373
 
BlpI GC'TnA_GC

Cuts at:      0    1529    1936    2457 
   Size:       1529     407     521

  Fragments arranged by size: 

                1529     521     407
 
Bme1580I G_kGCm'C

Cuts at:      0    1134    1965    2162    2457 
   Size:       1134     831     197     295

  Fragments arranged by size: 

                1134     831     295     197
 
BmgBI CAC'GTC

Cuts at:      0     152     696    2457 
   Size:        152     544    1761

  Fragments arranged by size: 

                1761     544     152
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      84    2457 
   Size:         84    2373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1627    1805    1936    2457 
   Size:       1627     178     131     521

  Fragments arranged by size: 

                1627     521     178     131
 
Bpu10I CC'TnA_GC

Cuts at:      0     374    1537    2457 
   Size:        374    1163     920

  Fragments arranged by size: 

                1163     920     374
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     461     805    2012    2272    2457 
   Size:        289     172     344    1207     260     185

  Fragments arranged by size: 

                1207     344     289     260     185     172
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     200     980    2457 
   Size:        200     780    1477

  Fragments arranged by size: 

                1477     780     200
 
BsaHI Gr'CG_yC

Cuts at:      0     178     461    1193    2457 
   Size:        178     283     732    1264

  Fragments arranged by size: 

                1264     732     283     178
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     382     412    2457 
   Size:        382      30    2045

  Fragments arranged by size: 

                2045     382      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     178     391    1525    1582    2457 
   Size:        178     213    1134      57     875

  Fragments arranged by size: 

                1134     875     213     178      57
 
BseYI C'CCAG_C

Cuts at:      0     224     842     846    1022    1032    1092    2321    2457 
   Size:        224     618       4     176      10      60    1229     136

  Fragments arranged by size: 

                1229     618     224     176     136      60      10       4
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     418    2455    2457 
   Size:        266     152    2037       2

  Fragments arranged by size: 

                2037     266     152       2
 
BsiHKAI G_wGCw'C

Cuts at:      0      26    1530    2457 
   Size:         26    1504     927

  Fragments arranged by size: 

                1504     927      26
 
BsmI GAATG_Cn'

Cuts at:      0    1905    2457 
   Size:       1905     552
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     154    2457 
   Size:        154    2303
 
Bsp1286I G_dGCh'C

Cuts at:      0      26    1023    1134    1530    1942    1965    2028    2162 
   Size:         26     997     111     396     412      23      63     134

Cuts at:   2162    2457 
   Size:        295

  Fragments arranged by size: 

                 997     412     396     295     134     111      63      26
                  23
 
BspHI T'CATG_A

Cuts at:      0    2450    2457 
   Size:       2450       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     608     998    2457 
   Size:        608     390    1459

  Fragments arranged by size: 

                1459     608     390
 
BsrBI CCG'CTC

Cuts at:      0    1449    2457 
   Size:       1449    1008
 
BsrDI GCAATG_nn'

Cuts at:      0    2006    2457 
   Size:       2006     451
 
BsrFI r'CCGG_y

Cuts at:      0     708    2457 
   Size:        708    1749
 
BsrGI T'GTAC_A

Cuts at:      0    1601    2457 
   Size:       1601     856
 
BssSI C'ACGA_G

Cuts at:      0    1543    2457 
   Size:       1543     914
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     441     718    1426    2457 
   Size:        441     277     708    1031

  Fragments arranged by size: 

                1031     708     441     277
 
BstBI TT'CG_AA

Cuts at:      0     879    2457 
   Size:        879    1578
 
BstEII G'GTnAC_C

Cuts at:      0    1552    2457 
   Size:       1552     905
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1549    2457 
   Size:       1549     908
 
BstYI r'GATC_y

Cuts at:      0     104    1149    2457 
   Size:        104    1045    1308

  Fragments arranged by size: 

                1308    1045     104
 
Bsu36I CC'TnA_GG

Cuts at:      0    1135    1660    2457 
   Size:       1135     525     797

  Fragments arranged by size: 

                1135     797     525
 
BtgI C'CryG_G

Cuts at:      0     674    1622    2457 
   Size:        674     948     835

  Fragments arranged by size: 

                 948     835     674
 
BtsI GCAGTG_nn'

Cuts at:      0     439    2457 
   Size:        439    2018
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     240     275    2457 
   Size:        240      35    2182

  Fragments arranged by size: 

                2182     240      35
 
DraI TTT'AAA

Cuts at:      0    1044    1508    2457 
   Size:       1044     464     949

  Fragments arranged by size: 

                1044     949     464
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     518    2457 
   Size:        518    1939
 
EarI CTCTTCn'nnn_

Cuts at:      0     338     349    1862    2177    2457 
   Size:        338      11    1513     315     280

  Fragments arranged by size: 

                1513     338     315     280      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1234    2457 
   Size:       1234    1223
 
EcoICRI GAG'CTC

Cuts at:      0      24    2457 
   Size:         24    2433
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      13     325     745    1267    1516    1627    1805    1936 
   Size:         13     312     420     522     249     111     178     131

Cuts at:   1936    2008    2457 
   Size:         72     449

  Fragments arranged by size: 

                 522     449     420     312     249     178     131     111
                  72      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2167    2457 
   Size:       2167     290
 
EcoO109I rG'GnC_Cy

Cuts at:      0     359     511    1130    1131    1638    2318    2445    2457 
   Size:        359     152     619       1     507     680     127      12

  Fragments arranged by size: 

                 680     619     507     359     152     127      12       1
            
 
EcoRI G'AATT_C

Cuts at:      0     428    2457 
   Size:        428    2029
 
EcoRV GAT'ATC

Cuts at:      0    2136    2457 
   Size:       2136     321
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     378     481    2263    2457 
   Size:        378     103    1782     194

  Fragments arranged by size: 

                1782     378     194     103
 
FspI TGC'GCA

Cuts at:      0     751    2457 
   Size:        751    1706
 
FspAI rTGC'GCAy

Cuts at:      0     751    2457 
   Size:        751    1706
 
HaeII r_GCGC'y

Cuts at:      0     126     961    2148    2457 
   Size:        126     835    1187     309

  Fragments arranged by size: 

                1187     835     309     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1178    1210    2329    2361    2457 
   Size:       1178      32    1119      32      96

  Fragments arranged by size: 

                1178    1119      96      32      32
 
HincII GTy'rAC

Cuts at:      0     704    2457 
   Size:        704    1753
 
MfeI C'AATT_G

Cuts at:      0     871    2457 
   Size:        871    1586
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     686    1313    1997    2457 
   Size:        686     627     684     460

  Fragments arranged by size: 

                 686     684     627     460
 
MslI CAynn'nnrTG

Cuts at:      0     544     811    2457 
   Size:        544     267    1646

  Fragments arranged by size: 

                1646     544     267
 
MspA1I CmG'CkG

Cuts at:      0     846    1032    2457 
   Size:        846     186    1425

  Fragments arranged by size: 

                1425     846     186
 
NcoI C'CATG_G

Cuts at:      0     674    2457 
   Size:        674    1783
 
NspI r_CATG'y

Cuts at:      0     669     978    1695    2457 
   Size:        669     309     717     762

  Fragments arranged by size: 

                 762     717     669     309
 
PciI A'CATG_T

Cuts at:      0     665    1691    2457 
   Size:        665    1026     766

  Fragments arranged by size: 

                1026     766     665
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     231    2457 
   Size:        231    2226
 
PfoI T'CCnGG_A

Cuts at:      0     349    1055    2457 
   Size:        349     706    1402

  Fragments arranged by size: 

                1402     706     349
 
PpuMI rG'GwC_Cy

Cuts at:      0     359     511    2445    2457 
   Size:        359     152    1934      12

  Fragments arranged by size: 

                1934     359     152      12
 
PsiI TTA'TAA

Cuts at:      0     797    2457 
   Size:        797    1660
 
PspOMI G'GGCC_C

Cuts at:      0    1130    2457 
   Size:       1130    1327
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      80     112    2457 
   Size:         80      32    2345

  Fragments arranged by size: 

                2345      80      32
 
PstI C_TGCA'G

Cuts at:      0     605    2457 
   Size:        605    1852
 
PvuII CAG'CTG

Cuts at:      0     846    1032    2457 
   Size:        846     186    1425

  Fragments arranged by size: 

                1425     846     186
 
SacI G_AGCT'C

Cuts at:      0      26    2457 
   Size:         26    2431
 
SanDI GG'GwC_CC

Cuts at:      0     511    2457 
   Size:        511    1946
 
ScaI AGT'ACT

Cuts at:      0    1648    2457 
   Size:       1648     809
 
SexAI A'CCwGG_T

Cuts at:      0     611    1048    2457 
   Size:        611     437    1409

  Fragments arranged by size: 

                1409     611     437
 
SfcI C'TryA_G

Cuts at:      0     601     922    2457 
   Size:        601     321    1535

  Fragments arranged by size: 

                1535     601     321
 
SmlI C'TyrA_G

Cuts at:      0     268     476     784    2027    2287    2457 
   Size:        268     208     308    1243     260     170

  Fragments arranged by size: 

                1243     308     268     260     208     170
 
SpeI A'CTAG_T

Cuts at:      0    1804    2457 
   Size:       1804     653
 
SphI G_CATG'C

Cuts at:      0     978    2457 
   Size:        978    1479
 
StuI AGG'CCT

Cuts at:      0     772    2457 
   Size:        772    1685
 
StyI C'CwwG_G

Cuts at:      0     674     773    1630    2457 
   Size:        674      99     857     827

  Fragments arranged by size: 

                 857     827     674      99
 
TatI w'GTAC_w

Cuts at:      0    1061    1169    1601    1646    2457 
   Size:       1061     108     432      45     811

  Fragments arranged by size: 

                1061     811     432     108      45
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1225    1257    2457 
   Size:       1225      32    1200

  Fragments arranged by size: 

                1225    1200      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2427    2457 
   Size:       2427      30
 
XmnI GAAnn'nnTTC

Cuts at:      0    2243    2457 
   Size:       2243     214
 
ZraI GAC'GTC

Cuts at:      0    1194    2457 
   Size:       1194    1263
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AleI     AloI     ApaI
     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BcgI     BclI
     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
    CspCI     DraI     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     MfeI     MmeI     MslI   MspA1I     NcoI     NspI     PciI
    PflMI     PfoI    PpuMI     PsiI   PspOMI     PsrI     PstI    PvuII
     SacI    SanDI     ScaI    SexAI     SfcI     SmlI     SpeI     SphI
     StuI     StyI     TatI     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI    AlwNI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BciVI    BfrBI     BglI    BglII     BmrI     BmtI    BsaAI    BsaBI
    BsaWI    BsiEI    BsiWI    BspEI   BssHII  BstZ17I    BtgZI     ClaI
   DraIII     EaeI     EagI     FseI  HindIII     HpaI     KasI     KpnI
     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
    PspXI     PvuI    RsrII    SacII     SalI     SapI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI
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