Restriction map for pF1KA0094
 (Linear) (Six-base) MAPSORT of: KIAA0094.seq  Check: 3553  from: 1  to: 1158



KIAA0094.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     509    1158 
   Size:        419      90     649

  Fragments arranged by size: 

                 649     419      90
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     549    1158 
   Size:        549     609
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1096    1158 
   Size:       1096      62
 
ApaLI G'TGCA_C

Cuts at:      0    1130    1158 
   Size:       1130      28
 
AseI AT'TA_AT

Cuts at:      0     218    1158 
   Size:        218     940
 
BanII G_rGCy'C

Cuts at:      0      97    1158 
   Size:         97    1061
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     185    1158 
   Size:        185     973
 
BclI T'GATC_A

Cuts at:      0     327    1158 
   Size:        327     831
 
BglI GCCn_nnn'nGGC

Cuts at:      0     288    1158 
   Size:        288     870
 
Bme1580I G_kGCm'C

Cuts at:      0    1134    1158 
   Size:       1134      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43    1158 
   Size:         43    1115
 
BseYI C'CCAG_C

Cuts at:      0      81    1158 
   Size:         81    1077
 
BsiEI CG_ry'CG

Cuts at:      0       8    1158 
   Size:          8    1150
 
BsiHKAI G_wGCw'C

Cuts at:      0     735    1076    1134    1158 
   Size:        735     341      58      24

  Fragments arranged by size: 

                 735     341      58      24
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       7    1040    1158 
   Size:          7    1033     118

  Fragments arranged by size: 

                1033     118       7
 
Bsp1286I G_dGCh'C

Cuts at:      0      97     735    1076    1134    1158 
   Size:         97     638     341      58      24

  Fragments arranged by size: 

                 638     341      97      58      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     226    1158 
   Size:        226     932
 
BsrDI GCAATG_nn'

Cuts at:      0     681    1158 
   Size:        681     477
 
BsrFI r'CCGG_y

Cuts at:      0     460    1158 
   Size:        460     698
 
BsrGI T'GTAC_A

Cuts at:      0     507    1158 
   Size:        507     651
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     523    1158 
   Size:        523     635
 
BstBI TT'CG_AA

Cuts at:      0     864    1158 
   Size:        864     294
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     847    1028    1158 
   Size:        847     181     130

  Fragments arranged by size: 

                 847     181     130
 
Bsu36I CC'TnA_GG

Cuts at:      0     336    1158 
   Size:        336     822
 
BtgI C'CryG_G

Cuts at:      0       8     229     606    1158 
   Size:          8     221     377     552

  Fragments arranged by size: 

                 552     377     221       8
 
BtsI GCAGTG_nn'

Cuts at:      0      51     791    1158 
   Size:         51     740     367

  Fragments arranged by size: 

                 740     367      51
 
ClaI AT'CG_AT

Cuts at:      0     243    1158 
   Size:        243     915
 
DraI TTT'AAA

Cuts at:      0     123    1158 
   Size:        123    1035
 
EaeI y'GGCC_r

Cuts at:      0       5    1018    1158 
   Size:          5    1013     140

  Fragments arranged by size: 

                1013     140       5
 
EagI C'GGCC_G

Cuts at:      0       5    1158 
   Size:          5    1153
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1013    1158 
   Size:       1013     145
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     419     509    1158 
   Size:         43     376      90     649

  Fragments arranged by size: 

                 649     376      90      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     626     690    1158 
   Size:        626      64     468

  Fragments arranged by size: 

                 626     468      64
 
EcoRV GAT'ATC

Cuts at:      0     660    1158 
   Size:        660     498
 
HindIII A'AGCT_T

Cuts at:      0     892    1158 
   Size:        892     266
 
MfeI C'AATT_G

Cuts at:      0     974    1158 
   Size:        974     184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     693     924    1009    1158 
   Size:        693     231      85     149

  Fragments arranged by size: 

                 693     231     149      85
 
MscI TGG'CCA

Cuts at:      0    1020    1158 
   Size:       1020     138
 
MslI CAynn'nnrTG

Cuts at:      0     759    1158 
   Size:        759     399
 
NaeI GCC'GGC

Cuts at:      0     462    1158 
   Size:        462     696
 
NcoI C'CATG_G

Cuts at:      0     229     606    1158 
   Size:        229     377     552

  Fragments arranged by size: 

                 552     377     229
 
NdeI CA'TA_TG

Cuts at:      0     756    1158 
   Size:        756     402
 
NgoMIV G'CCGG_C

Cuts at:      0     460    1158 
   Size:        460     698
 
NspI r_CATG'y

Cuts at:      0     521     838    1158 
   Size:        521     317     320

  Fragments arranged by size: 

                 521     320     317
 
PfoI T'CCnGG_A

Cuts at:      0     196    1158 
   Size:        196     962
 
PpuMI rG'GwC_Cy

Cuts at:      0     690    1158 
   Size:        690     468
 
PsiI TTA'TAA

Cuts at:      0     560    1158 
   Size:        560     598
 
PstI C_TGCA'G

Cuts at:      0      43    1158 
   Size:         43    1115
 
SexAI A'CCwGG_T

Cuts at:      0     474    1158 
   Size:        474     684
 
SfcI C'TryA_G

Cuts at:      0      39    1158 
   Size:         39    1119
 
SphI G_CATG'C

Cuts at:      0     838    1158 
   Size:        838     320
 
StyI C'CwwG_G

Cuts at:      0     229     606    1158 
   Size:        229     377     552

  Fragments arranged by size: 

                 552     377     229
 
TatI w'GTAC_w

Cuts at:      0     507    1158 
   Size:        507     651
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    ApaLI     AseI    BanII     BbsI     BclI
     BglI Bme1580I     BpmI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstBI    BstXI   Bsu36I
     BtgI     BtsI     ClaI     DraI     EaeI     EagI     EciI  Eco57MI
 EcoO109I    EcoRV  HindIII     MfeI     MmeI     MscI     MslI     NaeI
     NcoI     NdeI   NgoMIV     NspI     PfoI    PpuMI     PsiI     PstI
    SexAI     SfcI     SphI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiWI     BsmI    BspEI    BspHI    BsrBI   BssHII
    BssSI   BstEII    BstYI  BstZ17I    BtgZI    CspCI   DraIII     DrdI
     EarI  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI   MspA1I
     NarI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com