Restriction map for pF1KA0092
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BglI        
BglII       BlnI        BmyI        BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HhaI        HinP1I      HincII      HindII      
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MluI        MluNI       
MroI        MscI        MslI        MspI        MvnI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
TspRI       Tth111I     XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcyI            9  GGCGTC               G[GA]^CG[CT]C
AfaI          319  GTAC                 GT^AC
AflII         217  CTTAAG               C^TTAAG
AflIII        540  ACATGT               A^C[GA][CT]GT
AlwI          567  GGATC                GGATC(4/5)
AseI          988  ATTAAT               AT^TAAT
AsnI          988  ATTAAT               AT^TAAT
AvaII        1295  GGACC                G^G[AT]CC
BfrI          217  CTTAAG               C^TTAAG
BlpI           55  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       55  GCTGAGC              GC^T[ACGT]AGC
BsaHI           9  GGCGTC               G[GA]^CG[CT]C
BsaI         1326  GAGACC               GGTCTC(1/5)
BsaJI         827  CCGTGG               C^C[ACGT][ACGT]GG
BsiYI         822  CCATGCCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          822  CCATGCCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI        1294  GGGAC                GGGAC(10/14)
BsmI           90  GCATTC               GAATGC(1/-1)
BspLU11I      540  ACATGT               A^CATGT
BsrDI         887  GCAATG               GCAATG(2/0)
BssSI         935  CACGAG               CACGAG(-5/-1)
BstBI        1175  TTCGAA               TT^CGAA
BstYI         567  GGATCT               [GA]^GATC[CT]
CelII          55  GCTGAGC              GC^T[ACGT]AGC
DraII         608  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        935  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          827  CCGTGG               C^C[GA][CT]GG
EcoO109I      608  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HaeIII        609  GGCC                 GG^CC
Hin1I           9  GGCGTC               G[GA]^CG[CT]C
HindIII       878  AAGCTT               A^AGCTT
HphI         1053  TCACC                GGTGA(8/7)
MaeIII        964  GTAAC                ^GT[ACGT]AC
MflI          567  GGATCT               [GA]^GATC[CT]
NdeI          104  CATATG               CA^TATG
NlaIV        1294  GGGACC               GG[ACGT]^[ACGT]CC
NspV         1175  TTCGAA               TT^CGAA
PflMI         822  CCATGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         483  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         988  ATTAAT               AT^TAAT
PstI          404  CTGCAG               CTGCA^G
RsaI          319  GTAC                 GT^AC
ScaI          318  AGTACT               AGT^ACT
SfaNI         855  GCATC                GCATC(5/9)
SfcI          404  CTGCAG               C^T[GA][CT]AG
SfuI         1175  TTCGAA               TT^CGAA
Van91I        822  CCATGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         567  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BclI          531  TGATCA               T^GATCA
             1050  TGATCA              
BpmI         1195  CTGGAG               CTGGAG(16/14)
             1331  CTGGAG              
BseRI         975  CTCCTC               GAGGAG(10/8)
              994  GAGGAG              
BsmAI        1326  GAGAC                GTCTC(1/5)
               16  GTCTC               
BsrI          321  ACTGG                ACTGG(1/-1)
              706  ACTGG               
BssKI        1077  CCAGG                ^CC[ACGT]GG
             1330  CCTGG               
BstNI        1077  CCAGG                CC^[AT]GG
             1330  CCTGG               
Cfr13I        609  GGCCC                G^G[ACGT]CC
             1295  GGACC               
EcoRI         348  GAATTC               G^AATTC
             1386  GAATTC              
EcoRII       1077  CCAGG                ^CC[AT]GG
             1330  CCTGG               
FbaI          531  TGATCA               T^GATCA
             1050  TGATCA              
MfeI          302  CAATTG               C^AATTG
              436  CAATTG              
MspA1I        692  CAGCTG               C[AC]G^C[GT]G
             1192  CAGCTG              
MunI          302  CAATTG               C^AATTG
              436  CAATTG              
MvaI         1077  CCAGG                CC^[AT]GG
             1330  CCTGG               
PvuII         692  CAGCTG               CAG^CTG
             1192  CAGCTG              
Sau96I        609  GGCCC                G^G[ACGT]CC
             1295  GGACC               
ScrFI        1077  CCAGG                CC^[ACGT]GG
             1330  CCTGG               
SmlI          217  CTTAAG               C^T[CT][GA]AG
              253  CTTGAG              
SphI          465  GCATGC               GCATG^C
              677  GCATGC              
XbaI          292  TCTAGA               T^CTAGA
              711  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BfaI        BstF5I      Cac8I       DdeI        DpnII       
Fnu4HI      FokI        HgaI        HinfI       ItaI        
MaeI        MboI        MboII       MnlI        MseI        
MwoI        NdeII       NlaIII      NspI        PleI        
Sau3AI      TaqI        TfiI        Tru9I       TseI        
Tsp509I     TthHB8I     
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