Restriction map for pF1KA0092
 (Circular) (Six-base) MAPSORT of: pF1KA0092.seq  Check: 109  from: 1  to: 4535



pF1KA0092  4535 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:18  ..

AccI GT'mk_AC

Cuts at:   1573    1573 
   Size:       4535
 
Acc65I G'GTAC_C

Cuts at:   1551    1551 
   Size:       4535
 
AclI AA'CG_TT

Cuts at:   4375    4375 
   Size:       4535
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1447    2360    2792    3539    3807    1447 
   Size:        913     432     747     268    2175

  Fragments arranged by size: 

                2175     913     747     432     268
 
AflII C'TTAA_G

Cuts at:    325     325 
   Size:       4535
 
AflIII A'CryG_T

Cuts at:    648    1887    2991    3968     648 
   Size:       1239    1104     977    1215

  Fragments arranged by size: 

                1239    1215    1104     977
 
AgeI A'CCGG_T

Cuts at:   2923    2923 
   Size:       4535
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    737     737 
   Size:       4535
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    176     208     176 
   Size:         32    4503
 
AlwNI CAG_nnn'CTG

Cuts at:   1840    3407    1840 
   Size:       1567    2968
 
ApaLI G'TGCA_C

Cuts at:   3305    3305 
   Size:       4535
 
ApoI r'AATT_y

Cuts at:    456    1138    1494    1539    3888    4144     456 
   Size:        682     356      45    2349     256     847

  Fragments arranged by size: 

                2349     847     682     356     256      45
 
AseI AT'TA_AT

Cuts at:     20    1097      20 
   Size:       1077    3458
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4535
 
AvaI C'yCGr_G

Cuts at:   1555    1555 
   Size:       4535
 
BamHI G'GATC_C

Cuts at:   1560    1560 
   Size:       4535
 
BanI G'GyrC_C

Cuts at:   1551    1965    2213    2248    1551 
   Size:        414     248      35    3838

  Fragments arranged by size: 

                3838     414     248      35
 
BanII G_rGCy'C

Cuts at:   1549    2579    1549 
   Size:       1030    3505
 
BbeI G_GCGC'C

Cuts at:   1969    2217    1969 
   Size:        248    4287
 
BciVI GTATCCnnnnn_n'

Cuts at:   2433    3194    4535    2433 
   Size:        761    1341    2433

  Fragments arranged by size: 

                2433    1341     761
 
BclI T'GATC_A

Cuts at:    639    1158    2055     639 
   Size:        519     897    3119

  Fragments arranged by size: 

                3119     897     519
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       4535
 
BglII A'GATC_T

Cuts at:   2050    4087    2050 
   Size:       2037    2498
 
BlpI GC'TnA_GC

Cuts at:    164    1647     164 
   Size:       1483    3052
 
Bme1580I G_kGCm'C

Cuts at:   2160    2253    3309    2160 
   Size:         93    1056    3386

  Fragments arranged by size: 

                3386    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1747    1828    2163    4307    1747 
   Size:         81     335    2144    1975

  Fragments arranged by size: 

                2144    1975     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2902    2902 
   Size:       4535
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1324    1460    1324 
   Size:        136    4399
 
Bpu10I CC'TnA_GC

Cuts at:    794     794 
   Size:       4535
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    382    1711    2590    3082    3380     382 
   Size:       1329     879     492     298    1537

  Fragments arranged by size: 

                1537    1329     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1428    4239    1428 
   Size:       2811    1724
 
BsaAI yAC'GTr

Cuts at:   1888    1888 
   Size:       4535
 
BsaBI GATnn'nnATC

Cuts at:      6    2049    4086       6 
   Size:       2043    2037     455

  Fragments arranged by size: 

                2043    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    118    1966    2214    2916    4412     118 
   Size:       1848     248     702    1496     241

  Fragments arranged by size: 

                1848    1496     702     248     241
 
BsaWI w'CCGG_w

Cuts at:   1724    2245    2923    3197    3344    3755    1724 
   Size:        521     678     274     147     411    2504

  Fragments arranged by size: 

                2504     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1074    1117    1074 
   Size:         43    4492
 
BseYI C'CCAG_C

Cuts at:   1298    1960    2718    3295    3791    1298 
   Size:        662     758     577     496    2042

  Fragments arranged by size: 

                2042     758     662     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    2077    2123    3331     104 
   Size:       1973      46    1208    1308

  Fragments arranged by size: 

                1973    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1549    2327    2517    3309    1549 
   Size:        778     190     792    2775

  Fragments arranged by size: 

                2775     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    198     198 
   Size:       4535
 
Bsp1286I G_dGCh'C

Cuts at:   1549    2160    2253    2327    2517    2579    3309    1549 
   Size:        611      93      74     190      62     730    2775

  Fragments arranged by size: 

                2775     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3755    3755 
   Size:       4535
 
BspHI T'CATG_A

Cuts at:   3711    3711 
   Size:       4535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1585    1799    2101    2482    1585 
   Size:        214     302     381    3638

  Fragments arranged by size: 

                3638     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2827    2881    4365    4530      50 
   Size:       2777      54    1484     165      55

  Fragments arranged by size: 

                2777    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1002    2447    1002 
   Size:       1445    3090
 
BsrFI r'CCGG_y

Cuts at:   2533    2714    2923    2533 
   Size:        181     209    4145

  Fragments arranged by size: 

                4145     209     181
 
BssSI C'ACGA_G

Cuts at:   1043    2806    3164    1043 
   Size:       1763     358    2414

  Fragments arranged by size: 

                2414    1763     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1912    1912 
   Size:       4535
 
BstBI TT'CG_AA

Cuts at:   1284    2896    1284 
   Size:       1612    2923
 
BstYI r'GATC_y

Cuts at:      1     675    1560    2027    2050    2385    2631    3643    3729 
   Size:        674     885     467      23     335     246    1012      86

Cuts at:   3729    3741    4087       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     885     674     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     935     107 
   Size:        828    3707
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2485    2666    2829    2485 
   Size:        181     163    4191

  Fragments arranged by size: 

                4191     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1778    1778 
   Size:       4535
 
DraI TTT'AAA

Cuts at:   1534    1534 
   Size:       4535
 
DraIII CAC_nnn'GTG

Cuts at:   1048    1048 
   Size:       4535
 
DrdI GACnn_nn'nnGTC

Cuts at:   2241    3099    2241 
   Size:        858    3677
 
EaeI y'GGCC_r

Cuts at:   2120    2294    2685    2712    4057    2120 
   Size:        174     391      27    1345    2598

  Fragments arranged by size: 

                2598    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2120    2120 
   Size:       4535
 
EarI CTCTTCn'nnn_

Cuts at:   2558    2768    2558 
   Size:        210    4325
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2743    3053    3199    4345    2743 
   Size:        310     146    1146    2933

  Fragments arranged by size: 

                2933    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1547    1547 
   Size:       4535
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1324    1447    1460    2360    2792    3539    3807    1324 
   Size:        123      13     900     432     747     268    2052

  Fragments arranged by size: 

                2052     900     747     432     268     123      13
 
EcoO109I rG'GnC_Cy

Cuts at:    717    1674     717 
   Size:        957    3578
 
EcoRI G'AATT_C

Cuts at:    456    1494    1539     456 
   Size:       1038      45    3452

  Fragments arranged by size: 

                3452    1038      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2554    2554 
   Size:       4535
 
FspI TGC'GCA

Cuts at:   2316    2316 
   Size:       4535
 
HaeII r_GCGC'y

Cuts at:   1969    2217    3239    4218    1969 
   Size:        248    1022     979    2286

  Fragments arranged by size: 

                2286    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    577     609    1093    1125    1143    1175    1443    1475    2377 
   Size:         32     484      32      18      32     268      32     902

Cuts at:   2377    2407    2409    2439     577 
   Size:         30       2      30    2673

  Fragments arranged by size: 

                2673     902     484     268      32      32      32      32
                  30      30      18       2
 
HincII GTy'rAC

Cuts at:   1574    1574 
   Size:       4535
 
HindIII A'AGCT_T

Cuts at:    986     986 
   Size:       4535
 
KasI G'GCGC_C

Cuts at:   1965    2213    1965 
   Size:        248    4287
 
KpnI G_GTAC'C

Cuts at:   1555    1555 
   Size:       4535
 
MfeI C'AATT_G

Cuts at:    410     544     410 
   Size:        134    4401
 
MluI A'CGCG_T

Cuts at:   3968    3968 
   Size:       4535
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    725    1112    1199    3206    3390     725 
   Size:        387      87    2007     184    1870

  Fragments arranged by size: 

                2007    1870     387     184      87
 
MscI TGG'CCA

Cuts at:   2296    2296 
   Size:       4535
 
MslI CAynn'nnrTG

Cuts at:   2524    2651    2524 
   Size:        127    4408
 
MspA1I CmG'CkG

Cuts at:    802    1302    1646    1960    2320    3333    3578     802 
   Size:        500     344     314     360    1013     245    1759

  Fragments arranged by size: 

                1759    1013     500     360     344     314     245
 
NaeI GCC'GGC

Cuts at:   2716    2716 
   Size:       4535
 
NarI GG'CG_CC

Cuts at:   1966    2214    1966 
   Size:        248    4287
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4535
 
NdeI CA'TA_TG

Cuts at:    213     213 
   Size:       4535
 
NgoMIV G'CCGG_C

Cuts at:   2714    2714 
   Size:       4535
 
NspI r_CATG'y

Cuts at:    558     577     652     789    1482    1588    2088    2995     558 
   Size:         19      75     137     693     106     500     907    2098

  Fragments arranged by size: 

                2098     907     693     500     137     106      75      19
            
 
PciI A'CATG_T

Cuts at:    648    2991     648 
   Size:       2343    2192
 
PflMI CCAn_nnn'nTGG

Cuts at:    936     936 
   Size:       4535
 
PfoI T'CCnGG_A

Cuts at:   1184    1184 
   Size:       4535
 
PmeI GTTT'AAAC

Cuts at:   1534    1534 
   Size:       4535
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3674    3706    3674 
   Size:         32    4503
 
PshAI GACnn'nnGTC

Cuts at:    595     595 
   Size:       4535
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    932     964     932 
   Size:         32    4503
 
PstI C_TGCA'G

Cuts at:    516    1582    2267     516 
   Size:       1066     685    2784

  Fragments arranged by size: 

                2784    1066     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4535
 
PvuII CAG'CTG

Cuts at:    802    1302    1960    2320     802 
   Size:        500     658     360    3017

  Fragments arranged by size: 

                3017     658     500     360
 
RsrII CG'GwC_CG

Cuts at:   2730    2730 
   Size:       4535
 
SacI G_AGCT'C

Cuts at:   1549    1549 
   Size:       4535
 
SalI G'TCGA_C

Cuts at:   1572    1572 
   Size:       4535
 
SapI GCTCTTCn'nnn_

Cuts at:   2558    2768    2558 
   Size:        210    4325
 
SbfI CC_TGCA'GG

Cuts at:   1582    1582 
   Size:       4535
 
ScaI AGT'ACT

Cuts at:    428    4052     428 
   Size:       3624     911
 
SfcI C'TryA_G

Cuts at:     33     512    1578    2263    3256    3447      33 
   Size:        479    1066     685     993     191    1121

  Fragments arranged by size: 

                1121    1066     993     685     479     191
 
SfoI GGC'GCC

Cuts at:   1967    2215    1967 
   Size:        248    4287
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4535
 
SmaI CCC'GGG

Cuts at:   1557    1557 
   Size:       4535
 
SmlI C'TyrA_G

Cuts at:    325     361    1690    2605    3097    3359     325 
   Size:         36    1329     915     492     262    1501

  Fragments arranged by size: 

                1501    1329     915     492     262      36
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4535
 
SphI G_CATG'C

Cuts at:    577     789    1588    2088     577 
   Size:        212     799     500    3024

  Fragments arranged by size: 

                3024     799     500     212
 
StyI C'CwwG_G

Cuts at:    107    1669    2023     107 
   Size:       1562     354    2619

  Fragments arranged by size: 

                2619    1562     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2118    2251    2710    2919    2118 
   Size:        133     459     209    3734

  Fragments arranged by size: 

                3734     459     209     133
 
TatI w'GTAC_w

Cuts at:    426    4050     426 
   Size:       3624     911
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4052    4084    4052 
   Size:         32    4503
 
Tth111I GACn'n_nGTC

Cuts at:   2332    2332 
   Size:       4535
 
XbaI T'CTAG_A

Cuts at:    400     819    1566     400 
   Size:        419     747    3369

  Fragments arranged by size: 

                3369     747     419
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2030    2030 
   Size:       4535
 
XmaI C'CCGG_G

Cuts at:   1555    1555 
   Size:       4535
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     ApaI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BsaXI
     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI    EcoNI    EcoRV     FseI    FspAI     HpaI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI    PpuMI
     PsiI   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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