Restriction map for pF1KA0092
 (Linear) (Six-base) MAPSORT of: KIAA0092.seq  Check: 5010  from: 1  to: 1422



KIAA0092.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1339    1422 
   Size:       1339      83
 
AflII C'TTAA_G

Cuts at:      0     217    1422 
   Size:        217    1205
 
AflIII A'CryG_T

Cuts at:      0     540    1422 
   Size:        540     882
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     629    1422 
   Size:        629     793
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      68     100    1422 
   Size:         68      32    1322

  Fragments arranged by size: 

                1322      68      32
 
ApoI r'AATT_y

Cuts at:      0     348    1030    1386    1422 
   Size:        348     682     356      36

  Fragments arranged by size: 

                 682     356     348      36
 
AseI AT'TA_AT

Cuts at:      0     989    1422 
   Size:        989     433
 
BclI T'GATC_A

Cuts at:      0     531    1050    1422 
   Size:        531     519     372

  Fragments arranged by size: 

                 531     519     372
 
BlpI GC'TnA_GC

Cuts at:      0      56    1422 
   Size:         56    1366
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1216    1352    1422 
   Size:       1216     136      70

  Fragments arranged by size: 

                1216     136      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     686    1422 
   Size:        686     736
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274    1422 
   Size:        274    1148
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1320    1422 
   Size:       1320     102
 
BsaHI Gr'CG_yC

Cuts at:      0      10    1422 
   Size:         10    1412
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     966    1009    1422 
   Size:        966      43     413

  Fragments arranged by size: 

                 966     413      43
 
BseYI C'CCAG_C

Cuts at:      0    1190    1422 
   Size:       1190     232
 
BsmI GAATG_Cn'

Cuts at:      0      90    1422 
   Size:         90    1332
 
BsrDI GCAATG_nn'

Cuts at:      0     894    1422 
   Size:        894     528
 
BssSI C'ACGA_G

Cuts at:      0     935    1422 
   Size:        935     487
 
BstBI TT'CG_AA

Cuts at:      0    1176    1422 
   Size:       1176     246
 
BstYI r'GATC_y

Cuts at:      0     567    1422 
   Size:        567     855
 
BtgI C'CryG_G

Cuts at:      0     827    1422 
   Size:        827     595
 
DraIII CAC_nnn'GTG

Cuts at:      0     940    1422 
   Size:        940     482
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1216    1339    1352    1422 
   Size:       1216     123      13      70

  Fragments arranged by size: 

                1216     123      70      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     609    1422 
   Size:        609     813
 
EcoRI G'AATT_C

Cuts at:      0     348    1386    1422 
   Size:        348    1038      36

  Fragments arranged by size: 

                1038     348      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501     985    1017    1035    1067    1335    1367 
   Size:        469      32     484      32      18      32     268      32

Cuts at:   1367    1422 
   Size:         55

  Fragments arranged by size: 

                 484     469     268      55      32      32      32      32
                  18
 
HindIII A'AGCT_T

Cuts at:      0     878    1422 
   Size:        878     544
 
MfeI C'AATT_G

Cuts at:      0     302     436    1422 
   Size:        302     134     986

  Fragments arranged by size: 

                 986     302     134
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     617    1004    1091    1422 
   Size:        617     387      87     331

  Fragments arranged by size: 

                 617     387     331      87
 
MspA1I CmG'CkG

Cuts at:      0     694    1194    1422 
   Size:        694     500     228

  Fragments arranged by size: 

                 694     500     228
 
NdeI CA'TA_TG

Cuts at:      0     105    1422 
   Size:        105    1317
 
NspI r_CATG'y

Cuts at:      0     450     469     544     681    1374    1422 
   Size:        450      19      75     137     693      48

  Fragments arranged by size: 

                 693     450     137      75      48      19
 
PciI A'CATG_T

Cuts at:      0     540    1422 
   Size:        540     882
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     828    1422 
   Size:        828     594
 
PfoI T'CCnGG_A

Cuts at:      0    1076    1422 
   Size:       1076     346
 
PshAI GACnn'nnGTC

Cuts at:      0     487    1422 
   Size:        487     935
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     824     856    1422 
   Size:        824      32     566

  Fragments arranged by size: 

                 824     566      32
 
PstI C_TGCA'G

Cuts at:      0     408    1422 
   Size:        408    1014
 
PvuII CAG'CTG

Cuts at:      0     694    1194    1422 
   Size:        694     500     228

  Fragments arranged by size: 

                 694     500     228
 
ScaI AGT'ACT

Cuts at:      0     320    1422 
   Size:        320    1102
 
SfcI C'TryA_G

Cuts at:      0     404    1422 
   Size:        404    1018
 
SmlI C'TyrA_G

Cuts at:      0     217     253    1422 
   Size:        217      36    1169

  Fragments arranged by size: 

                1169     217      36
 
SphI G_CATG'C

Cuts at:      0     469     681    1422 
   Size:        469     212     741

  Fragments arranged by size: 

                 741     469     212
 
TatI w'GTAC_w

Cuts at:      0     318    1422 
   Size:        318    1104
 
XbaI T'CTAG_A

Cuts at:      0     292     711    1422 
   Size:        292     419     711

  Fragments arranged by size: 

                 711     419     292
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI     AloI     ApoI     AseI     BclI
     BlpI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BseRI    BseYI
     BsmI    BsrDI    BssSI    BstBI    BstYI     BtgI   DraIII  Eco57MI
 EcoO109I    EcoRI    Hin4I  HindIII     MfeI     MmeI   MspA1I     NdeI
     NspI     PciI    PflMI     PfoI    PshAI     PsrI     PstI    PvuII
     ScaI     SfcI     SmlI     SphI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MluI     MscI     MslI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
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