Restriction map for pF1KB9867
 (Linear) (Six-base) MAPSORT of: KIBB9867.seq  Check: 5030  from: 1  to: 1338



KIBB9867.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     167    1338 
   Size:        167    1171
 
AatII G_ACGT'C

Cuts at:      0    1289    1338 
   Size:       1289      49
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1338 
   Size:        832     506
 
AflIII A'CryG_T

Cuts at:      0     941    1338 
   Size:        941     397
 
AlwNI CAG_nnn'CTG

Cuts at:      0     250    1338 
   Size:        250    1088
 
BanII G_rGCy'C

Cuts at:      0    1189    1338 
   Size:       1189     149
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     820    1338 
   Size:        820     518
 
BbvCI CC'TCA_GC

Cuts at:      0     568    1338 
   Size:        568     770
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      79    1338 
   Size:         79    1259
 
BclI T'GATC_A

Cuts at:      0     347     428     827    1338 
   Size:        347      81     399     511

  Fragments arranged by size: 

                 511     399     347      81
 
BglII A'GATC_T

Cuts at:      0     207    1338 
   Size:        207    1131
 
BlpI GC'TnA_GC

Cuts at:      0     481    1338 
   Size:        481     857
 
BmgBI CAC'GTC

Cuts at:      0     942    1338 
   Size:        942     396
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      44     466    1338 
   Size:         44     422     872

  Fragments arranged by size: 

                 872     422      44
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     546    1315    1338 
   Size:        360     186     769      23

  Fragments arranged by size: 

                 769     360     186      23
 
Bpu10I CC'TnA_GC

Cuts at:      0     568    1338 
   Size:        568     770
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     535    1338 
   Size:        535     803
 
BsaHI Gr'CG_yC

Cuts at:      0    1286    1338 
   Size:       1286      52
 
BsaWI w'CCGG_w

Cuts at:      0     386     765    1338 
   Size:        386     379     573

  Fragments arranged by size: 

                 573     386     379
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     544     574    1231    1261    1338 
   Size:        544      30     657      30      77

  Fragments arranged by size: 

                 657     544      77      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1179    1225    1338 
   Size:       1179      46     113

  Fragments arranged by size: 

                1179     113      46
 
BseYI C'CCAG_C

Cuts at:      0    1251    1338 
   Size:       1251      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1338 
   Size:        496     842
 
BsiHKAI G_wGCw'C

Cuts at:      0     319    1338 
   Size:        319    1019
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     898    1069    1293    1338 
   Size:        898     171     224      45

  Fragments arranged by size: 

                 898     224     171      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     319    1189    1338 
   Size:        319     870     149

  Fragments arranged by size: 

                 870     319     149
 
BspHI T'CATG_A

Cuts at:      0     626    1122    1338 
   Size:        626     496     216

  Fragments arranged by size: 

                 626     496     216
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     167    1338 
   Size:        167    1171
 
BsrDI GCAATG_nn'

Cuts at:      0     680    1338 
   Size:        680     658
 
BssSI C'ACGA_G

Cuts at:      0    1130    1338 
   Size:       1130     208
 
BstEII G'GTnAC_C

Cuts at:      0     171    1338 
   Size:        171    1167
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     114     534    1236    1338 
   Size:        114     420     702     102

  Fragments arranged by size: 

                 702     420     114     102
 
BstYI r'GATC_y

Cuts at:      0     207     647    1301    1338 
   Size:        207     440     654      37

  Fragments arranged by size: 

                 654     440     207      37
 
BtgI C'CryG_G

Cuts at:      0      23    1338 
   Size:         23    1315
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1120    1209    1338 
   Size:       1120      89     129

  Fragments arranged by size: 

                1120     129      89
 
BtsI GCAGTG_nn'

Cuts at:      0      74     251     320    1338 
   Size:         74     177      69    1018

  Fragments arranged by size: 

                1018     177      74      69
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1285    1338 
   Size:       1285      53
 
EaeI y'GGCC_r

Cuts at:      0     218     620    1338 
   Size:        218     402     718

  Fragments arranged by size: 

                 718     402     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     546     832    1315    1338 
   Size:        360     186     286     483      23

  Fragments arranged by size: 

                 483     360     286     186      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     642     693    1204    1338 
   Size:        642      51     511     134

  Fragments arranged by size: 

                 642     511     134      51
 
EcoO109I rG'GnC_Cy

Cuts at:      0     278    1197    1338 
   Size:        278     919     141

  Fragments arranged by size: 

                 919     278     141
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     435     804    1338 
   Size:        435     369     534

  Fragments arranged by size: 

                 534     435     369
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1124    1156    1231    1263    1338 
   Size:       1124      32      75      32      75

  Fragments arranged by size: 

                1124      75      75      32      32
 
HindIII A'AGCT_T

Cuts at:      0     603    1338 
   Size:        603     735
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     953    1329    1338 
   Size:        177     776     376       9

  Fragments arranged by size: 

                 776     376     177       9
 
MscI TGG'CCA

Cuts at:      0     622    1338 
   Size:        622     716
 
MslI CAynn'nnrTG

Cuts at:      0     295     472     616     835    1338 
   Size:        295     177     144     219     503

  Fragments arranged by size: 

                 503     295     219     177     144
 
MspA1I CmG'CkG

Cuts at:      0     485    1219    1338 
   Size:        485     734     119

  Fragments arranged by size: 

                 734     485     119
 
NcoI C'CATG_G

Cuts at:      0      23    1338 
   Size:         23    1315
 
NdeI CA'TA_TG

Cuts at:      0     579    1338 
   Size:        579     759
 
NspI r_CATG'y

Cuts at:      0    1180    1338 
   Size:       1180     158
 
PpuMI rG'GwC_Cy

Cuts at:      0     278    1197    1338 
   Size:        278     919     141

  Fragments arranged by size: 

                 919     278     141
 
PvuII CAG'CTG

Cuts at:      0     485    1219    1338 
   Size:        485     734     119

  Fragments arranged by size: 

                 734     485     119
 
SfcI C'TryA_G

Cuts at:      0     651    1041    1338 
   Size:        651     390     297

  Fragments arranged by size: 

                 651     390     297
 
StyI C'CwwG_G

Cuts at:      0      23     167     194     410     689    1145    1235    1338 
   Size:         23     144      27     216     279     456      90     103

  Fragments arranged by size: 

                 456     279     216     144     103      90      27      23
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     285     823     947    1251    1338 
   Size:        285     538     124     304      87

  Fragments arranged by size: 

                 538     304     285     124      87
 
TatI w'GTAC_w

Cuts at:      0     453    1338 
   Size:        453     885
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     148     180    1338 
   Size:        148      32    1158

  Fragments arranged by size: 

                1158     148      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     447     819    1338 
   Size:        447     372     519

  Fragments arranged by size: 

                 519     447     372
 
ZraI GAC'GTC

Cuts at:      0    1287    1338 
   Size:       1287      51
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII    AlwNI    BanII     BbsI    BbvCI
    BciVI     BclI    BglII     BlpI    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BssSI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI  Eco57MI    EcoNI
 EcoO109I     FalI    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NspI    PpuMI    PvuII     SfcI     StyI    TaqII
     TatI     TstI  Tth111I     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BfrBI
     BglI Bme1580I     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BspEI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     XbaI     XcmI     XhoI     XmaI     XmnI
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