Restriction map for pF1KA0041
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AgeI        AhdI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BclI        BcnI        BfrI        BlnI        BsaAI       
BsaBI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Cfr10I      ClaI        CpoI        DraII       DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoT22I     
FbaI        FseI        FspI        HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII       1312  TCCGGA               T^CCGGA
AcyI         1836  GGCGTC               G[GA]^CG[CT]C
AflIII       1966  ACGTGT               A^C[GA][CT]GT
Alw44I        662  GTGCAC               G^TGCAC
AlwNI         855  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          936  CCTCAGG              CC^T[ACGT]AGG
ApaLI         662  GTGCAC               G^TGCAC
AseI          529  ATTAAT               AT^TAAT
AsnI          529  ATTAAT               AT^TAAT
Asp700I       372  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1642  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         2089  GGTGCC               G^G[CT][GA]CC
BbvCI        1867  GCTGAGG              CCTCAGC(-5/-2)
BcgI          976  CGACGATTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1220  GTATCC               GTATCC(6/5)
BglI          592  GCCCATTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         345  AGATCT               A^GATCT
BlpI         1614  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1614  GCTGAGC              GC^T[ACGT]AGC
BsaHI        1836  GGCGTC               G[GA]^CG[CT]C
BseAI        1312  TCCGGA               T^CCGGA
BseRI          22  GAGGAG               GAGGAG(10/8)
BsgI         1886  GTGCAG               GTGCAG(16/14)
BspEI        1312  TCCGGA               T^CCGGA
BspHI         176  TCATGA               T^CATGA
BspMI        2064  GCAGGT               ACCTGC(4/8)
BstAPI       2149  GCAGCCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         379  TTCGAA               TT^CGAA
Bsu36I        936  CCTCAGG              CC^T[ACGT]AGG
CelII        1614  GCTGAGC              GC^T[ACGT]AGC
DraI         1389  TTTAAA               TTT^AAA
DraIII       1293  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        936  CCTCAGG              CC^T[ACGT]AGG
EcoRV         812  GATATC               GAT^ATC
HaeII         857  GGCGCT               [GA]GCGC^[CT]
HgaI         1837  GCGTC                GACGC(5/10)
Hin1I        1836  GGCGTC               G[GA]^CG[CT]C
HincII       1999  GTCAAC               GT[CT]^[GA]AC
HindII       1999  GTCAAC               GT[CT]^[GA]AC
MroI         1312  TCCGGA               T^CCGGA
NdeI         1841  CATATG               CA^TATG
NspV          379  TTCGAA               TT^CGAA
PflFI        1642  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         529  ATTAAT               AT^TAAT
PvuII        1236  CAGCTG               CAG^CTG
RcaI          176  TCATGA               T^CATGA
SacI         1701  GAGCTC               GAGCT^C
SfcI         1981  CTACAG               C^T[GA][CT]AG
SfuI          379  TTCGAA               TT^CGAA
SphI         1016  GCATGC               GCATG^C
SspI         1544  AATATT               AAT^ATT
Tth111I      1642  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          372  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AfaI          430  GTAC                 GT^AC
             1351  GTAC                
BanII        1701  GAGCTC               G[GA]GC[CT]^C
             1953  GGGCTC              
BfaI          209  CTAG                 C^TAG
             1153  CTAG                
BpmI          750  CTCCAG               CTGGAG(16/14)
             1021  CTCCAG              
BsaI          191  GAGACC               GGTCTC(1/5)
              996  GGTCTC              
BsaWI        1312  TCCGGA               [AT]^CCGG[AT]
             1322  ACCGGA              
BsmFI        1640  GGGAC                GGGAC(10/14)
             2025  GGGAC               
DsaI         1264  CCACGG               C^C[GA][CT]GG
             1733  CCACGG              
EarI         1715  GAAGAG               CTCTTC(1/4)
             2197  GAAGAG              
EcoRI        1316  GAATTC               G^AATTC
             1691  GAATTC              
EcoT14I      1329  CCTTGG               C^C[AT][AT]GG
             2016  CCAAGG              
HapII        1313  CCGG                 C^CGG
             1323  CCGG                
HindIII       109  AAGCTT               A^AGCTT
             1393  AAGCTT              
HpaII        1313  CCGG                 C^CGG
             1323  CCGG                
Ksp632I      1715  GAAGAG               CTCTTC(1/4)
             2197  GAAGAG              
MaeI          209  CTAG                 C^TAG
             1153  CTAG                
MslI          798  CATAGTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2136  CATAGCTGTG          
MspA1I       1207  CAGCGG               C[AC]G^C[GT]G
             1236  CAGCTG              
MspI         1313  CCGG                 C^CGG
             1323  CCGG                
NspI         1016  GCATGC               [GA]CATG^[CT]
             2100  ACATGC              
RsaI          430  GTAC                 GT^AC
             1351  GTAC                
StyI         1329  CCTTGG               C^C[AT][AT]GG
             2016  CCAAGG              
XcmI         1215  CCAGTGTATCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1274  CCAGCATCAACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AspHI       AvaII       BbsI        BbvI        BmyI        
BpuAI       BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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