Restriction map for pF1KA0001
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        TaqI        Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflII         652  CTTAAG               C^TTAAG
AlwI          231  GATCC                GGATC(4/5)
Asp700I       828  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         295  GTGCTC               G[AT]GC[AT]^C
BalI          575  TGGCCA               TGG^CCA
BanII         337  GGGCTC               G[GA]GC[CT]^C
BbsI           94  GTCTTC               GAAGAC(2/6)
BfrI          652  CTTAAG               C^TTAAG
BpmI           26  CTCCAG               CTGGAG(16/14)
BpuAI          94  GTCTTC               GAAGAC(2/6)
BsaAI         145  TACGTG               [CT]AC^GT[GA]
BsaI          285  GGTCTC               GGTCTC(1/5)
BseRI         448  CTCCTC               GAGGAG(10/8)
BsgI          279  GTGCAG               GTGCAG(16/14)
BsiHKAI       295  GTGCTC               G[AT]GC[AT]^C
BsiYI         578  CCATCTTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          578  CCATCTTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         286  GTCTC                GTCTC(1/5)
BspLU11I      311  ACATGT               A^CATGT
BsrDI          99  CATTGC               GCAATG(2/0)
BssKI         257  CCTGG                ^CC[ACGT]GG
BstNI         257  CCTGG                CC^[AT]GG
BstYI         230  AGATCC               [GA]^GATC[CT]
Cac8I         896  GCCAGC               GC[ACGT]^[ACGT]GC
EaeI          575  TGGCCA               [CT]^GGCC[GA]
EarI          298  CTCTTC               CTCTTC(1/4)
EcoRII        257  CCTGG                ^CC[AT]GG
HincII        307  GTCAAC               GT[CT]^[GA]AC
HindII        307  GTCAAC               GT[CT]^[GA]AC
Ksp632I       298  CTCTTC               CTCTTC(1/4)
MflI          230  AGATCC               [GA]^GATC[CT]
MluNI         575  TGGCCA               TGG^CCA
MscI          575  TGGCCA               TGG^CCA
MvaI          257  CCTGG                CC^[AT]GG
NspI          311  ACATGT               [GA]CATG^[CT]
PflMI         578  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI          297  GCTCTTC              GCTCTTC(1/4)
ScrFI         257  CCTGG                CC^[ACGT]GG
SspI          466  AATATT               AAT^ATT
StuI          246  AGGCCT               AGG^CCT
Tsp45I        239  GTGAC                ^GT[GC]AC
Van91I        578  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         230  AGATCC               [GA]^GATC[CT]
XmnI          828  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI          696  CCGC                 CCGC(-3/-1)
              819  GCGG                
AcsI          666  GAATTC               [GA]^AATT[CT]
              832  GAATTC              
AflIII        269  ACGTGT               A^C[GA][CT]GT
              311  ACATGT              
ApoI          666  GAATTC               [GA]^AATT[CT]
              832  GAATTC              
AvaII         253  GGTCC                G^G[AT]CC
              870  GGACC               
BclI            2  TGATCA               T^GATCA
               56  TGATCA              
BfaI          692  CTAG                 C^TAG
              958  CTAG                
Cfr13I        253  GGTCC                G^G[ACGT]CC
              870  GGACC               
EcoRI         666  GAATTC               G^AATTC
              832  GAATTC              
EcoT14I       234  CCTTGG               C^C[AT][AT]GG
              249  CCTTGG              
FbaI            2  TGATCA               T^GATCA
               56  TGATCA              
HaeIII        247  GGCC                 GG^CC
              576  GGCC                
HphI          238  GGTGA                GGTGA(8/7)
              476  TCACC               
MaeI          692  CTAG                 C^TAG
              958  CTAG                
MslI           72  CATTCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              567  CATCTTCGTG          
MspA1I        262  CAGCTG               C[AC]G^C[GT]G
              795  CAGCTG              
NlaIII        312  CATG                 CATG^
              444  CATG                
NlaIV         253  GGTCCC               GG[ACGT]^[ACGT]CC
              870  GGACCC              
PleI          241  GACTC                GAGTC(4/5)
              774  GAGTC               
PvuII         262  CAGCTG               CAG^CTG
              795  CAGCTG              
Sau96I        253  GGTCC                G^G[ACGT]CC
              870  GGACC               
SmlI          178  CTCAAG               C^T[CT][GA]AG
              652  CTTAAG              
StyI          234  CCTTGG               C^C[AT][AT]GG
              249  CCTTGG              
TspRI         401  CAGTG                CA[GC]TG[ACGT][ACGT]^
              425  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AfaI        AluI        BbvI        BmyI        BsaJI       
BsmFI       Bsp1286I    BsrI        BstF5I      DdeI        
DpnII       Fnu4HI      FokI        HinfI       ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        NdeII       RsaI        Sau3AI      SfaNI       
TfiI        Tru9I       TseI        Tsp509I     
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