(Circular) (Six-base) MAPSORT of: pFN21AE3898.seq Check: 7082 from: 1 to: 5632 pFN21AE3898 5632 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2501 3442 278 Size: 53 83 186 936 965 941 2468 Fragments arranged by size: 2468 965 941 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2466 2975 1937 Size: 529 509 4594 Fragments arranged by size: 4594 529 509 Acc65I G'GTAC_C Cuts at: 2526 2953 2526 Size: 427 5205 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3695 5079 5347 1632 Size: 2063 1384 268 1917 Fragments arranged by size: 2063 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AlwNI CAG_nnn'CTG Cuts at: 2092 3375 4947 2092 Size: 1283 1572 2777 Fragments arranged by size: 2777 1572 1283 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2941 3167 3319 5428 1493 Size: 1448 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 BamHI G'GATC_C Cuts at: 2779 2962 3329 2779 Size: 183 367 5082 Fragments arranged by size: 5082 367 183 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2526 2953 4408 618 Size: 325 217 627 227 512 427 1455 1842 Fragments arranged by size: 1842 1455 627 512 427 325 227 217 BanII G_rGCy'C Cuts at: 1391 2733 2951 1391 Size: 1342 218 4072 Fragments arranged by size: 4072 1342 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2162 2346 928 Size: 971 263 184 4214 Fragments arranged by size: 4214 971 263 184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 2427 2559 1548 Size: 879 132 4621 Fragments arranged by size: 4621 879 132 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 2375 3049 2375 Size: 674 4958 Bme1580I G_kGCm'C Cuts at: 1790 2775 3693 4849 1790 Size: 985 918 1156 2573 Fragments arranged by size: 2573 1156 985 918 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2464 3363 4321 Size: 416 53 83 399 24 1047 899 958 Cuts at: 4321 442 Size: 1753 Fragments arranged by size: 1753 1047 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2124 2422 2573 4292 1239 Size: 885 298 151 1719 2579 Fragments arranged by size: 2579 1719 885 298 151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2335 3423 493 Size: 711 1131 1088 2702 Fragments arranged by size: 2702 1131 1088 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2498 2829 3439 3821 Size: 53 83 186 936 965 331 610 382 Cuts at: 3821 4456 275 Size: 635 1451 Fragments arranged by size: 1451 965 936 635 610 382 331 186 83 53 BsaWI w'CCGG_w Cuts at: 2119 2523 2598 4065 4463 4737 4884 5295 2119 Size: 404 75 1467 398 274 147 411 2456 Fragments arranged by size: 2456 1467 411 404 398 274 147 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2720 2750 1178 Size: 30 165 30 1317 30 4060 Fragments arranged by size: 4060 1317 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2750 1186 Size: 838 726 4068 Fragments arranged by size: 4068 838 726 BseYI C'CCAG_C Cuts at: 2842 4835 5331 2842 Size: 1993 496 3143 Fragments arranged by size: 3143 1993 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5059 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2520 3082 3843 4871 1540 Size: 275 186 519 562 761 1028 2301 Fragments arranged by size: 2301 1028 761 562 519 275 186 BsiHKAI G_wGCw'C Cuts at: 2733 3693 3778 4849 2733 Size: 960 85 1071 3516 Fragments arranged by size: 3516 1071 960 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2733 2775 2951 3693 3778 4849 1391 Size: 399 943 42 176 742 85 1071 2174 Fragments arranged by size: 2174 1071 943 742 399 176 85 42 BspEI T'CCGG_A Cuts at: 2119 2598 5295 2119 Size: 479 2697 2456 Fragments arranged by size: 2697 2456 479 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 844 Size: 2143 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2108 2785 3519 1394 Size: 429 285 677 734 3507 Fragments arranged by size: 3507 734 677 429 285 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstXI CCAn_nnnn'nTGG Cuts at: 2311 2311 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2779 2962 3329 3714 3731 5183 5269 5281 Size: 807 183 367 385 17 1452 86 12 Cuts at: 5281 5627 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 807 385 367 346 183 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2233 513 Size: 552 939 229 3912 Fragments arranged by size: 3912 939 552 229 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 2562 3148 3942 3962 2562 Size: 586 794 20 4232 Fragments arranged by size: 4232 794 586 20 ClaI AT'CG_AT Cuts at: 1554 2280 2297 2547 1554 Size: 726 17 250 4639 Fragments arranged by size: 4639 726 250 17 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2750 4639 809 Size: 507 1434 1889 1802 Fragments arranged by size: 1889 1802 1434 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 3079 3968 Size: 54 1425 50 275 18 702 547 889 Cuts at: 3968 5597 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 702 547 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2274 2907 3562 1353 Size: 921 633 655 3423 Fragments arranged by size: 3423 921 655 633 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2731 2731 Size: 5632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2422 2573 3695 4292 5079 5347 Size: 393 492 298 151 1122 597 787 268 Cuts at: 5347 1239 Size: 1524 Fragments arranged by size: 1524 1122 787 597 492 393 298 268 151 EcoNI CCTnn'n_nnAGG Cuts at: 2243 2243 Size: 5632 EcoO109I rG'GnC_Cy Cuts at: 2408 2752 2408 Size: 344 5288 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 2046 2223 4779 1678 Size: 368 177 2556 2531 Fragments arranged by size: 2556 2531 368 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2544 2576 2692 2724 4273 Size: 32 135 32 972 32 116 32 1549 Cuts at: 4273 4305 4347 4379 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1549 972 135 116 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2467 2976 3222 669 Size: 988 281 529 509 246 3079 Fragments arranged by size: 3079 988 529 509 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2530 2957 2530 Size: 427 5205 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 4746 4930 1305 Size: 1176 2265 184 2007 Fragments arranged by size: 2265 2007 1176 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 10 Size: 54 1425 343 702 3108 Fragments arranged by size: 3108 1425 702 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 2645 3590 3949 4108 518 Size: 648 1381 98 945 359 159 2042 Fragments arranged by size: 2042 1381 945 648 359 159 98 MspA1I CmG'CkG Cuts at: 1606 2089 3048 3725 4873 5118 1606 Size: 483 959 677 1148 245 2120 Fragments arranged by size: 2120 1148 959 677 483 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2233 513 Size: 552 939 229 3912 Fragments arranged by size: 3912 939 552 229 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2746 1108 Size: 1638 3994 PfoI T'CCnGG_A Cuts at: 2589 2589 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2408 2752 2408 Size: 344 5288 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SacI G_AGCT'C Cuts at: 2733 2733 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2465 2974 1936 Size: 529 509 4594 Fragments arranged by size: 4594 529 509 SanDI GG'GwC_CC Cuts at: 2408 2408 Size: 5632 SapI GCTCTTCn'nnn_ Cuts at: 2274 2274 Size: 5632 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2130 2857 2980 4115 4796 4987 826 Size: 219 1085 727 123 1135 681 191 1471 Fragments arranged by size: 1471 1135 1085 727 681 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3736 4637 4899 820 Size: 197 926 1793 901 262 1553 Fragments arranged by size: 1793 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2233 3071 513 Size: 552 939 229 838 3074 Fragments arranged by size: 3074 939 838 552 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2506 3659 3676 3829 4014 4459 325 Size: 1255 926 1153 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1153 926 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3878 5590 96 Size: 274 80 33 51 1443 1901 1712 138 Fragments arranged by size: 1901 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2182 2214 2720 2752 5592 5624 2182 Size: 32 506 32 2840 32 2190 Fragments arranged by size: 2840 2190 506 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2272 2968 2272 Size: 696 4936 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2231 2644 2231 Size: 413 5219 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2499 3440 276 Size: 53 83 186 936 965 941 2468 Fragments arranged by size: 2468 965 941 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI