################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI FseI HgaI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 1710 CGCG CG^CG AcyI 92 GGCGCC G[GA]^CG[CT]C AflII 1662 CTTAAG C^TTAAG AlwNI 1802 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AviII 14 TGCGCA TGC^GCA BanII 872 GGGCTC G[GA]GC[CT]^C BbeI 92 GGCGCC GGCGC^C BbsI 1217 GTCTTC GAAGAC(2/6) BclI 1317 TGATCA T^GATCA BfaI 1295 CTAG C^TAG BfrI 1662 CTTAAG C^TTAAG BglI 528 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1285 CTCCAG CTGGAG(16/14) BpuAI 1217 GTCTTC GAAGAC(2/6) BsaHI 92 GGCGCC G[GA]^CG[CT]C BsaI 1048 GAGACC GGTCTC(1/5) BsaWI 1058 ACCGGA [AT]^CCGG[AT] BseRI 1627 CTCCTC GAGGAG(10/8) BsgI 1469 GTGCAG GTGCAG(16/14) BsmAI 1048 GAGAC GTCTC(1/5) BsrBI 22 GAGCGG CCGCTC(-3/-3) BstUI 1710 CGCG CG^CG BstYI 300 GGATCT [GA]^GATC[CT] DraI 262 TTTAAA TTT^AAA DraIII 1594 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 649 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1426 GATATC GAT^ATC EcoT22I 1823 ATGCAT ATGCA^T FbaI 1317 TGATCA T^GATCA FspI 14 TGCGCA TGC^GCA Hin1I 92 GGCGCC G[GA]^CG[CT]C HincII 1561 GTTGAC GT[CT]^[GA]AC HindII 1561 GTTGAC GT[CT]^[GA]AC KasI 92 GGCGCC G^GCGCC MaeI 1295 CTAG C^TAG MflI 300 GGATCT [GA]^GATC[CT] MvnI 1710 CGCG CG^CG NarI 92 GGCGCC GG^CGCC NsiI 1823 ATGCAT ATGCA^T PstI 1966 CTGCAG CTGCA^G SfcI 1966 CTGCAG C^T[GA][CT]AG SfoI 92 GGCGCC GGC^GCC SmlI 1662 CTTAAG C^T[CT][GA]AG SspI 1225 AATATT AAT^ATT XhoII 300 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 619 GTAC GT^AC 1253 GTAC AlwI 117 GATCC GGATC(4/5) 300 GGATC Aor51HI 362 AGCGCT AGC^GCT 910 AGCGCT AvaI 79 CCCGAG C^[CT]CG[GA]G 869 CTCGGG BalI 1004 TGGCCA TGG^CCA 1411 TGGCCA BanI 92 GGCGCC G^G[CT][GA]CC 920 GGCACC BsaAI 462 CACGTA [CT]AC^GT[GA] 793 TACGTG BsoBI 79 CCCGAG C^[CT]CG[GA]G 869 CTCGGG BspHI 1277 TCATGA T^CATGA 1736 TCATGA DsaI 830 CCATGG C^C[GA][CT]GG 1418 CCATGG Eco47III 362 AGCGCT AGC^GCT 910 AGCGCT EcoT14I 830 CCATGG C^C[AT][AT]GG 1418 CCATGG MaeII 463 ACGT A^CGT 794 ACGT MluNI 1004 TGGCCA TGG^CCA 1411 TGGCCA MscI 1004 TGGCCA TGG^CCA 1411 TGGCCA MspA1I 802 CCGCTG C[AC]G^C[GT]G 1121 CCGCTG NcoI 830 CCATGG C^CATGG 1418 CCATGG RcaI 1277 TCATGA T^CATGA 1736 TCATGA RsaI 619 GTAC GT^AC 1253 GTAC StyI 830 CCATGG C^C[AT][AT]GG 1418 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AcsI AluI Alw44I ApaLI ApoI Asp700I AspHI AvaII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PpuMI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmnI