(Circular) (Six-base) MAPSORT of: pFN21AB9440.seq Check: 9764 from: 1 to: 6685 pFN21AB9440 6685 bp With 182 enzymes: SgfI * May 17, 2016 16:10 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4495 278 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4028 1937 Size: 2091 4594 Acc65I G'GTAC_C Cuts at: 4006 4006 Size: 6685 AclI AA'CG_TT Cuts at: 1563 4812 5185 1563 Size: 3249 373 3063 Fragments arranged by size: 3249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3155 3713 3983 4748 6132 6400 1632 Size: 1523 558 270 765 1384 268 1917 Fragments arranged by size: 1917 1523 1384 765 558 270 268 AfeI AGC'GCT Cuts at: 2369 2917 2369 Size: 548 6137 AflII C'TTAA_G Cuts at: 820 1017 3667 820 Size: 197 2650 3838 Fragments arranged by size: 3838 2650 197 AflIII A'CryG_T Cuts at: 1203 5584 6561 1203 Size: 4381 977 1327 Fragments arranged by size: 4381 1327 977 AgeI A'CCGG_T Cuts at: 5516 5516 Size: 6685 AhdI GACnn_n'nnGTC Cuts at: 5414 5414 Size: 6685 AleI CACnn'nnGTG Cuts at: 1166 2798 1166 Size: 1632 5053 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2342 1184 Size: 1158 5527 AlwNI CAG_nnn'CTG Cuts at: 3812 4428 6000 3812 Size: 616 1572 4497 Fragments arranged by size: 4497 1572 616 ApaLI G'TGCA_C Cuts at: 2279 2893 3329 4742 5898 2279 Size: 614 436 1413 1156 3066 Fragments arranged by size: 3066 1413 1156 614 436 ApoI r'AATT_y Cuts at: 1493 2272 2690 3639 3994 4220 4372 6481 1493 Size: 779 418 949 355 226 152 2109 1697 Fragments arranged by size: 2109 1697 949 779 418 355 226 152 AseI AT'TA_AT Cuts at: 160 1032 5239 160 Size: 872 4207 1606 Fragments arranged by size: 4207 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 2084 2874 4010 1870 Size: 73 141 790 1136 4545 Fragments arranged by size: 4545 1136 790 141 73 BamHI G'GATC_C Cuts at: 4015 4382 4015 Size: 367 6318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 2925 4006 5461 618 Size: 325 217 627 310 828 1081 1455 1842 Fragments arranged by size: 1842 1455 1081 828 627 325 310 217 BanII G_rGCy'C Cuts at: 1391 2881 4004 1391 Size: 1490 1123 4072 Fragments arranged by size: 4072 1490 1123 BbeI G_GCGC'C Cuts at: 2101 2101 Size: 6685 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3215 928 Size: 971 1316 4398 Fragments arranged by size: 4398 1316 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4876 4910 1903 Size: 34 2939 34 3678 Fragments arranged by size: 3678 2939 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4577 5787 4577 Size: 1210 5475 BclI T'GATC_A Cuts at: 1548 3322 1548 Size: 1774 4911 BfrBI ATG'CAT Cuts at: 3830 3830 Size: 6685 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2539 5296 136 Size: 107 122 71 631 605 867 2757 1525 Fragments arranged by size: 2757 1525 867 631 605 122 107 71 BglII A'GATC_T Cuts at: 6680 6680 Size: 6685 BlpI GC'TnA_GC Cuts at: 4102 4102 Size: 6685 Bme1580I G_kGCm'C Cuts at: 1790 2283 2897 3333 4746 5902 1790 Size: 493 614 436 1413 1156 2573 Fragments arranged by size: 2573 1413 1156 614 493 436 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6685 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4416 5374 Size: 416 53 83 399 24 1958 1041 958 Cuts at: 5374 442 Size: 1753 Fragments arranged by size: 1958 1753 1041 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3275 5345 1239 Size: 2036 2070 2579 Fragments arranged by size: 2579 2070 2036 Bpu10I CC'TnA_GC Cuts at: 2295 3108 2295 Size: 813 5872 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4810 5675 5973 4810 Size: 865 298 5522 Fragments arranged by size: 5522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3047 5348 882 Size: 617 1548 2301 2219 Fragments arranged by size: 2301 2219 1548 617 BsaAI yAC'GTr Cuts at: 493 1204 2469 2800 4476 493 Size: 711 1265 331 1676 2702 Fragments arranged by size: 2702 1676 1265 711 331 BsaBI GATnn'nnATC Cuts at: 6679 6679 Size: 6685 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2098 4492 4874 5509 Size: 53 83 186 936 565 2394 382 635 Cuts at: 5509 275 Size: 1451 Fragments arranged by size: 2394 1451 936 635 565 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3063 5118 5516 5790 5937 6348 3063 Size: 2055 398 274 147 411 3400 Fragments arranged by size: 3400 2055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3664 3694 1178 Size: 30 165 30 2261 30 4169 Fragments arranged by size: 4169 2261 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3623 1186 Size: 2437 4248 BseYI C'CCAG_C Cuts at: 2577 2982 5888 6384 2577 Size: 405 2906 496 2878 Fragments arranged by size: 2906 2878 496 405 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3495 1163 Size: 573 1759 4353 Fragments arranged by size: 4353 1759 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4135 4896 5924 1540 Size: 275 186 2134 761 1028 2301 Fragments arranged by size: 2301 2134 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 2897 3333 4746 4831 5902 2283 Size: 614 436 1413 85 1071 3066 Fragments arranged by size: 3066 1413 1071 614 436 85 BsmI GAATG_Cn' Cuts at: 4196 4289 4196 Size: 93 6592 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2881 2897 3333 4004 4746 4831 Size: 399 493 598 16 436 671 742 85 Cuts at: 4831 5902 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 671 598 493 436 399 85 16 BspEI T'CCGG_A Cuts at: 6348 6348 Size: 6685 BspHI T'CATG_A Cuts at: 3282 3741 4574 6304 3282 Size: 459 833 1730 3663 Fragments arranged by size: 3663 1730 833 459 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 3384 3394 4040 844 Size: 1181 1359 10 646 3489 Fragments arranged by size: 3489 1359 1181 646 10 BsrBI CCG'CTC Cuts at: 1394 1823 2029 4572 1394 Size: 429 206 2543 3507 Fragments arranged by size: 3507 2543 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 2898 3211 3592 5180 5354 65 Size: 1183 1650 313 381 1588 174 1396 Fragments arranged by size: 1650 1588 1396 1183 381 313 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5329 5516 1700 Size: 110 3519 187 2869 Fragments arranged by size: 3519 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6685 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6685 BssSI C'ACGA_G Cuts at: 4745 5757 4745 Size: 1012 5673 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6685 BstBI TT'CG_AA Cuts at: 5489 5489 Size: 6685 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6685 BstYI r'GATC_y Cuts at: 1972 2305 4015 4382 4767 4784 6236 6322 6334 Size: 333 1710 367 385 17 1452 86 12 Cuts at: 6334 6680 1972 Size: 346 1977 Fragments arranged by size: 1977 1710 1452 385 367 346 333 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2835 3423 513 Size: 552 1770 588 3775 Fragments arranged by size: 3775 1770 588 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2224 487 Size: 683 56 998 4948 Fragments arranged by size: 4948 998 683 56 BtsI GCAGTG_nn' Cuts at: 3012 3223 3968 4201 4995 5015 3012 Size: 211 745 233 794 20 4682 Fragments arranged by size: 4682 794 745 233 211 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6685 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2949 2984 3408 3443 529 Size: 35 2385 35 424 35 3771 Fragments arranged by size: 3771 2385 424 35 35 35 DraI TTT'AAA Cuts at: 2269 3989 2269 Size: 1720 4965 DraIII CAC_nnn'GTG Cuts at: 3604 3604 Size: 6685 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5692 809 Size: 507 4376 1802 Fragments arranged by size: 4376 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3009 3416 3868 Size: 54 1425 50 275 18 1179 407 452 Cuts at: 3868 4132 5021 6650 8 Size: 264 889 1629 43 Fragments arranged by size: 1629 1425 1179 889 452 407 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4132 1537 Size: 275 2320 4090 Fragments arranged by size: 4090 2320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4615 1353 Size: 3262 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2026 5272 5646 5792 387 Size: 1064 138 437 3246 374 146 1280 Fragments arranged by size: 3246 1280 1064 437 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3155 3275 3713 3983 4748 5345 6132 Size: 393 1523 120 438 270 765 597 787 Cuts at: 6132 6400 1239 Size: 268 1524 Fragments arranged by size: 1524 1523 787 765 597 438 393 270 268 120 EcoNI CCTnn'n_nnAGG Cuts at: 2658 2658 Size: 6685 EcoO109I rG'GnC_Cy Cuts at: 2643 2850 3091 2643 Size: 207 241 6237 Fragments arranged by size: 6237 241 207 EcoRI G'AATT_C Cuts at: 3994 4372 3994 Size: 378 6307 EcoRV GAT'ATC Cuts at: 3433 3433 Size: 6685 FspI TGC'GCA Cuts at: 2021 5191 2021 Size: 3170 3515 HaeII r_GCGC'y Cuts at: 1678 2101 2371 2919 5832 1678 Size: 423 270 548 2913 2531 Fragments arranged by size: 2913 2531 548 423 270 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 3664 3696 3736 Size: 32 135 32 970 32 1090 32 40 Cuts at: 3736 3768 5326 5358 5400 5432 1373 Size: 32 1558 32 42 32 2626 Fragments arranged by size: 2626 1558 1090 970 135 42 40 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3568 4029 4275 669 Size: 988 281 1630 461 246 3079 Fragments arranged by size: 3079 1630 988 461 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6685 HpaI GTT'AAC Cuts at: 4275 4275 Size: 6685 KasI G'GCGC_C Cuts at: 2097 2097 Size: 6685 KpnI G_GTAC'C Cuts at: 4010 4010 Size: 6685 MfeI C'AATT_G Cuts at: 4284 4284 Size: 6685 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3161 5799 5983 1305 Size: 1856 2638 184 2007 Fragments arranged by size: 2638 2007 1856 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3011 3418 10 Size: 54 1425 343 1179 407 3277 Fragments arranged by size: 3277 1425 1179 407 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2762 2798 3216 3281 4643 5002 Size: 648 1047 549 36 418 65 1362 359 Cuts at: 5002 5161 518 Size: 159 2042 Fragments arranged by size: 2042 1362 1047 648 549 418 359 159 65 36 MspA1I CmG'CkG Cuts at: 1606 2809 3128 4101 4778 5926 6171 1606 Size: 1203 319 973 677 1148 245 2120 Fragments arranged by size: 2120 1203 1148 973 677 319 245 NarI GG'CG_CC Cuts at: 2098 2098 Size: 6685 NcoI C'CATG_G Cuts at: 513 1065 2835 3423 513 Size: 552 1770 588 3775 Fragments arranged by size: 3775 1770 588 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6685 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 4132 4132 Size: 6685 NsiI A_TGCA'T Cuts at: 3832 3832 Size: 6685 NspI r_CATG'y Cuts at: 1132 1229 2323 2615 2761 3180 4043 5588 1132 Size: 97 1094 292 146 419 863 1545 2229 Fragments arranged by size: 2229 1545 1094 863 419 292 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6685 PciI A'CATG_T Cuts at: 5584 5584 Size: 6685 PflMI CCAn_nnn'nTGG Cuts at: 1108 2526 3034 3604 1108 Size: 1418 508 570 4189 Fragments arranged by size: 4189 1418 570 508 PfoI T'CCnGG_A Cuts at: 2070 3149 2070 Size: 1079 5606 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2643 2850 3091 2643 Size: 207 241 6237 Fragments arranged by size: 6237 241 207 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6685 PsiI TTA'TAA Cuts at: 4255 4255 Size: 6685 PstI C_TGCA'G Cuts at: 830 3975 4037 830 Size: 3145 62 3478 Fragments arranged by size: 3478 3145 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 SalI G'TCGA_C Cuts at: 1936 4027 1936 Size: 2091 4594 SanDI GG'GwC_CC Cuts at: 2643 2643 Size: 6685 SbfI CC_TGCA'GG Cuts at: 4037 4037 Size: 6685 ScaI AGT'ACT Cuts at: 4933 6645 4933 Size: 1712 4973 SfcI C'TryA_G Cuts at: 826 1045 3971 4033 5168 5849 6040 826 Size: 219 2926 62 1135 681 191 1471 Fragments arranged by size: 2926 1471 1135 681 219 191 62 SfoI GGC'GCC Cuts at: 2099 2099 Size: 6685 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 4012 1872 Size: 2140 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3667 4789 5690 5952 820 Size: 197 926 1724 1122 901 262 1553 Fragments arranged by size: 1724 1553 1122 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4476 493 Size: 3983 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 4043 1132 Size: 2911 3774 SspI AAT'ATT Cuts at: 5 52 3232 4609 5 Size: 47 3180 1377 2081 Fragments arranged by size: 3180 2081 1377 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6685 StyI C'CwwG_G Cuts at: 513 1065 2835 3423 4124 513 Size: 552 1770 588 701 3074 Fragments arranged by size: 3074 1770 701 588 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4712 4729 4882 5067 5512 325 Size: 1255 3132 17 153 185 445 1498 Fragments arranged by size: 3132 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4931 6643 96 Size: 274 80 33 51 1443 2954 1712 138 Fragments arranged by size: 2954 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3175 3207 3330 3362 6645 6677 3175 Size: 32 123 32 3283 32 3183 Fragments arranged by size: 3283 3183 123 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6685 XbaI T'CTAG_A Cuts at: 4021 4021 Size: 6685 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6685 XmaI C'CCGG_G Cuts at: 1870 4010 1870 Size: 2140 4545 XmnI GAAnn'nnTTC Cuts at: 2551 3140 3522 4814 2551 Size: 589 382 1292 4422 Fragments arranged by size: 4422 1292 589 382 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4493 276 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI